高产胞外多糖的空间诱变植物乳杆菌抗大鼠脂质过氧化功效研究
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摘要
目的研究神舟十号和十一号飞船两次搭载后筛选得到的高产胞外多糖(extracellular polysaccharide,EPS)空间诱变植物乳杆菌SS18-119的抗脂质过氧化功效。方法 48只SPF级大鼠随机分为高脂血症模型组和SS18-119菌株低、中、高剂量组,各组均饲喂高脂饲料以建立模型。模型组灌胃生理盐水,低、中、高剂量分别按照10~7、10~8、10~9 CFU/mL SS18-119灌胃8周。实验结束后,取大鼠肝脏组织进行病理学实验,测定大鼠血清和肝脏脂质含量、肝脏抗氧化相关指标。结果造模第28天,各组大鼠血脂含量显著升高,表明高脂血症模型建立成功。与模型组相比,高剂量组血清ALT活性显著降低(P<0.05),肝脏总胆固醇(total cholesterol,TC)含量显著降低46.6%(P<0.05),甘油三酯(triglyceride,TG)含量显著降低38.1%(P<0.01),低密度脂蛋白-C(low density lipoprotein,LDL-C)含量显著降低41.3%(P<0.05)。与模型组相比,高剂量组血清丙二醛(malondialdehyde,MDA)含量显著降低45.6%(P<0.01),超氧化物歧化酶(superoxide dismutase,SOD)活力显著提升6.9%(P<0.01),谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力显著提升2.6%(P<0.05),总抗氧化能力(total antioxidant capacity,T-AOC)显著提升5.6%(P<0.01)。油红O染色显示SS18-119菌株能够明显抑制脂肪肝,HE染色显示试验组肝脏病变程度明显减轻。结论空间诱变植物乳杆菌SS18-119高剂量组具有保护大鼠肝脏、抑制脂肪肝形成及较强的抗脂质过氧化功效。
Objective To determine the anti-lipid peroxidation effect of SS18-119,a space-mutated lactobacillus plantarum carried twice by Shenzhou 10 and 11 spacecraft,with high yield of extracellular polysaccharides and excellent in vitro antioxidant activity,in vivo with hyperlipemia(HLP)rat model.Methods Rats were randomly divided into model group,low,medium and high test substance groups of space-mutated lactobacillus plantarum SS18-119.The model group was given gastric physiological saline,and the low,medium and high test substance groups were given gastric gavage 107,108,and109 CFU/mL space mutagenesis plant lactobacillus SS18-119 strains.All groups were fed high-fat diet to establish hyperlipidemia model for 8 weeks.Serum,liver lipids and liver antioxidant related indexes were measured.Results There was a significant difference in blood lipid content in each group on 28 d compared with that on 0 d,indicating that the model of hyperlipidemia was successfully established.At the 8 th week,high test substance groups serum alanine aminotransferase activity was significantly lower than that of the model group(P<0.05).Compared with model group,liver total cholesterol in high test substance group was significantly reduced by 46.6%(P<0.05),and triglycerides were significantly reduced by 38.1%(P<0.01),low density lipoprotein-C content decreased significantly by41.3%(P<0.05).Compared with model group,serum MDA content in high test substance group decreased significantly by 45.6%(P<0.01),superoxide dismutase activity significantly increased by 6.9%(P<0.01),glutathione peroxidase activity significantly increased by 2.6%(P<0.05),the total antioxidant capacity significantly increased by 5.6%(P<0.01).Oil red O staining result showed that the high dose of test substance had the best inhibition of fatty liver,the HE staining result showed that the degree of liver lesions was significantly reduced in experimal groups.Conclusion The high dose of test substance from space mutagenesis lactobacillus plantarum SS18-119 has the ability of protecting liver,inhibiting fatty liver formation and anti-lipid peroxidation in hyperlipidemic rats.
Objective To determine the anti-lipid peroxidation effect of SS18-119,a space-mutated lactobacillus plantarum carried twice by Shenzhou 10 and 11 spacecraft,with high yield of extracellular polysaccharides and excellent in vitro antioxidant activity,in vivo with hyperlipemia(HLP)rat model.Methods Rats were randomly divided into model group,low,medium and high test substance groups of space-mutated lactobacillus plantarum SS18-119.The model group was given gastric physiological saline,and the low,medium and high test substance groups were given gastric gavage 107,108,and109 CFU/mL space mutagenesis plant lactobacillus SS18-119 strains.All groups were fed high-fat diet to establish hyperlipidemia model for 8 weeks.Serum,liver lipids and liver antioxidant related indexes were measured.Results There was a significant difference in blood lipid content in each group on 28 d compared with that on 0 d,indicating that the model of hyperlipidemia was successfully established.At the 8 th week,high test substance groups serum alanine aminotransferase activity was significantly lower than that of the model group(P<0.05).Compared with model group,liver total cholesterol in high test substance group was significantly reduced by 46.6%(P<0.05),and triglycerides were significantly reduced by 38.1%(P<0.01),low density lipoprotein-C content decreased significantly by41.3%(P<0.05).Compared with model group,serum MDA content in high test substance group decreased significantly by 45.6%(P<0.01),superoxide dismutase activity significantly increased by 6.9%(P<0.01),glutathione peroxidase activity significantly increased by 2.6%(P<0.05),the total antioxidant capacity significantly increased by 5.6%(P<0.01).Oil red O staining result showed that the high dose of test substance had the best inhibition of fatty liver,the HE staining result showed that the degree of liver lesions was significantly reduced in experimal groups.Conclusion The high dose of test substance from space mutagenesis lactobacillus plantarum SS18-119 has the ability of protecting liver,inhibiting fatty liver formation and anti-lipid peroxidation in hyperlipidemic rats.
引文
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[20]Hefnawy HT,Ramadan MF.Protective effects of Lactuca sativa ethanolic extract on carbon tetrachloride induced oxidative damage in rats[J].Asian Pac J Trop Dis,2013,3(4):277-285.
[21]Ramadan MF.Quercetin increases antioxidant activity of soy lecithin in a triolein model system[J].LWT-Food Sci Technol,2008,41(4):581-587.
[22]Xu RH,Shang N,Li PL,et al.In vitro and in vivo antioxidant activity of exopolysaccharide fractions from Bifidobacterium animalis RH[J].Anaerobe,2011,17(5):217-262.
[23]Zhang L,Liu C,Li D,et al.Antioxidant activity of an exopolysaccharide isolated from Lactobacillus plantarum C88[J].Biological Macromolecules,2013,54(1):270-275.
[24]Pan DD,Mei XM.Antioxidant activity of an exopolysaccharide purified from Lactococcus lactis subsp.lactis12[J].Carbohydrate Polymers,2010,80(3):908-914.
[2]Forstermann U,Xia N,Li H,et al.Roles of vascular oxidative stress and nitric oxide in the pathogenesis of atherosclerosis[J].Circulation Research,2017,120(4):713-735.
[3]刘佳,袁明,贾茗雯,等.模拟失重下氧化应激对EA.hy926细胞影响的转录组学研究[J].航天医学与医学工程,2018,31(6):583-589.Liu J,Yuan M,Jia MW,et al.Transcriptomics study on effects of oxidative stress on EA.hy926cells under simulated microgravity[J].Space Medicine&Medical Engineering,2018,31(6):583-589.
[4]Valko M,Leibfritz D,Moncol J,et al.Free radicals and antioxidants in normal physiological functions and human disease[J].Int J Biochem Cell Biol,2007,39(1):44-84.
[5]Caleja C,Barros L,AntonioA L,et al.A comparative study between natural and synthetic antioxidants:Evaluation of their performance after incorporation into biscuits[J].Food Chemistry,2016,216(1):342-346.
[6]Nakajima H,Suzuki Y,Kaizu H,et al.Cholesterol lowering activity of ropy fermented Milk[J].Food Science,1992,57(6):1327-1329.
[7]Kanmani P,Kumar RS,Yuvaraj N,et a1.Production and purification of a novel exopolysaccharide from lactic acid bacterium Streptococcus phocae PI80and its functional characteristics activity in vitro[J].Bioresource Technology,2011,102(7):4827-4833.
[8]Haroun BM,Refaat BM,Elmenoufy HA,et al.Structure analysis and antitumor activity of the exopolysaccharide from probiotic Lactobacillus plantarum NRRL B-4496in vitro and in vivo[J].Journal of Applied Science Research,2013,9(1):425-434.
[9]Guo SD,Mao WJ,Han J,et al.Structural characteristics and antioxidant activities of the extracellular polysaccharides produced by marine bacterium Edwardsiella tarda[J].Bioresource Technology,2010,101(12):4729-4732.
[10]Chen Y,Mao WJ,Yang YP,et al.Structure and antioxidant activity of an extracellular polysaccharide from coralassociated fungus,Aspergillus versicolor LCJ-5-4[J].Carbohydrate Polymers,2012,87(1):218-226.
[11]Fu L,Wang Y,Wang J,et al.Evaluation of the antioxidant activity of extracellular polysaccharides from Morchella esculenta[J].Food&Function,2013,4(6):871-879.
[12]刘慧.现代食品微生物学实验技术(第二版)[M].北京:中国轻工业出版社,2017:296-298.Liu H.Modern Food Microbiology Experimental Technology(Second Edition)[M].Beijing:China Light Industry Press,2017:296-298.
[13]刘慧,王世平,冉冉,等.藏灵菇源酸奶复合菌发酵剂对大鼠脂质过氧化的影响[J].中国食品学报,2010,10(3):27-32.Liu H,Wang SP,Ran R,et al.Effects of complex bacteria leaven from tibetan kefir on lipid peroxidation of rat[J].Chinese Journal of Food Science,2010,10(3):27-32.
[14]Li M,Shu XB,Xu HC,et al.Integrative analysis of metabolome and gut microbiota in diet-induced hyperlipidemic rats treated with berberine compounds[J].Journal of Translational Medicine,2016(14):1-13.
[15]雷婕.油红O和苏丹Ⅲ染色剂对脂肪样病变肝组织脂质染色效果的比较[J].医学信息,2015,(49):45-46.Lei J,Comparison of lipid staining effects of oil red O and SudanⅢstain on fatty liver tissue[J].Medical Information,2015,(49):45-46.
[16]聂彦芬,董牧群,刘慧,等.藏灵菇源副干酪乳杆菌KL1对蛋鸡脂质过氧化的研究[J].食品科学,2017,38(7):219-223.Nie YF,Dong MQ,Liu H,et a1.Study on lipid peroxidation in laying hens by tibetan mushroom source lactobacillus paracasei KL1[J].Food Science,2017,38(7):219-223.
[17]Pereira DI,Gibson GR.Effects of consumption of probiotics and prebiotics on serum lipid level in humans[J].Crit Rev Biochem Mol Biol,2008(29):259-281.
[18]Grotto D,Maria LS,Farina M,et a1.Importance of the lipid peroxidation biomarkers and methodological aspects for malondialdehyde quantification[J].Química Nova,2009,32(1):169-174.
[19]Nielsen F,Mikkelsen BB,Nielsen JB,et al.Plasma malondialdehyde as biomarker for oxidative stress:reference interval and effect of life-style factors[J].Clinic Chem,1997,43(7):1204-1214.
[20]Hefnawy HT,Ramadan MF.Protective effects of Lactuca sativa ethanolic extract on carbon tetrachloride induced oxidative damage in rats[J].Asian Pac J Trop Dis,2013,3(4):277-285.
[21]Ramadan MF.Quercetin increases antioxidant activity of soy lecithin in a triolein model system[J].LWT-Food Sci Technol,2008,41(4):581-587.
[22]Xu RH,Shang N,Li PL,et al.In vitro and in vivo antioxidant activity of exopolysaccharide fractions from Bifidobacterium animalis RH[J].Anaerobe,2011,17(5):217-262.
[23]Zhang L,Liu C,Li D,et al.Antioxidant activity of an exopolysaccharide isolated from Lactobacillus plantarum C88[J].Biological Macromolecules,2013,54(1):270-275.
[24]Pan DD,Mei XM.Antioxidant activity of an exopolysaccharide purified from Lactococcus lactis subsp.lactis12[J].Carbohydrate Polymers,2010,80(3):908-914.