贝那普利联合自体脾细胞治疗对肾小球肾炎大鼠免疫指标、肾功能和肾小球硬化的影响
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摘要
目的分析贝那普利联合自体脾细胞治疗对肾小球肾炎(GN)大鼠免疫指标、肾功能和肾小球硬化的影响。方法取30只清洁级SD大鼠随机分为对照组(给予生理盐水进行灌胃)、单药组(给予贝那普利水溶液进行灌胃)与联合组(贝那普利水溶液灌胃+输注自体脾细胞),三组各10只。比较三组大鼠疗后4周血肌酐、血尿素氮和CD4、CD8阳性细胞百分比的变化情况及肾小球硬化评分的差异。结果治疗4周后,联合组血肌酐和血尿素氮含量较单药组均显著下降(P <0. 05),且与对照组比较,差异无统计学意义(P> 0. 05)。单药组、联合组CD4和CD8阳性细胞百分率较对照组均显著升高,同时联合组较单药组显著升高(P <0. 05)。治疗后4周,联合组肾小球硬化评分较单药组显著下降,而单药组评分较对照组显著升高(P <0. 05)。结论贝那普利联合自体脾细胞治疗可有效改善GN大鼠肾功能,提高免疫功能,可有效阻滞肾小管损伤,减轻肾小球硬化,因此具有良好的临床应用前景。
Objective To analyze the effects of benazepril combined with autologous spleen cells on immune indexes,renal function and glomerulosclerosis in rats with glomerulonephritis( GN). Methods Thirty clean SD rats were randomly divided into control group( given normal saline for gastric lavage),single drug group( given benazepril solution for gastric lavage) and combined group( benazepril solution for gastric lavage + autologous splenocyte infusion),with 10 cases in each group. The changes of serum creatinine,blood urea nitrogen,percentage of CD4 and CD8 positive cells and the difference of glomerulosclerosis score in the three groups were compared 4 weeks after treatment. Results The serum creatinine and urea nitrogen levels in the combined group were significantly lower than those in the single drug group( P < 0. 05),and there was no significant difference compared with the control group( P > 0. 05). The percentage of CD4 and CD8 positive cells in single drug group and combination group was significantly higher than that in control group,and the percentage of CD4 and CD8 positive cells in combination group was significantly higher than that in single drug group( P < 0. 05). Four weeks after treatment,the glomerulosclerosis score of the combined group was significantly lower than that of the single drug group,while the score of the single drug group was significantly higher than that of the control group( P < 0. 05). Conclusion Benazepril combined with autologous spleen cells can effectively improve the renal function and immune function of GN rats,effectively block renal tubular injury and reduce glomerulosclerosis,so it has a good clinical application prospect.
Objective To analyze the effects of benazepril combined with autologous spleen cells on immune indexes,renal function and glomerulosclerosis in rats with glomerulonephritis( GN). Methods Thirty clean SD rats were randomly divided into control group( given normal saline for gastric lavage),single drug group( given benazepril solution for gastric lavage) and combined group( benazepril solution for gastric lavage + autologous splenocyte infusion),with 10 cases in each group. The changes of serum creatinine,blood urea nitrogen,percentage of CD4 and CD8 positive cells and the difference of glomerulosclerosis score in the three groups were compared 4 weeks after treatment. Results The serum creatinine and urea nitrogen levels in the combined group were significantly lower than those in the single drug group( P < 0. 05),and there was no significant difference compared with the control group( P > 0. 05). The percentage of CD4 and CD8 positive cells in single drug group and combination group was significantly higher than that in control group,and the percentage of CD4 and CD8 positive cells in combination group was significantly higher than that in single drug group( P < 0. 05). Four weeks after treatment,the glomerulosclerosis score of the combined group was significantly lower than that of the single drug group,while the score of the single drug group was significantly higher than that of the control group( P < 0. 05). Conclusion Benazepril combined with autologous spleen cells can effectively improve the renal function and immune function of GN rats,effectively block renal tubular injury and reduce glomerulosclerosis,so it has a good clinical application prospect.
引文
[1]Liu YH,Tsai YS,Lin SC,et al.Quantitative PPARγexpression affects the balance between tolerance and immunity[J].Sci Rep,2016,25(6):26646.
[2]曾彩虹.梅奥诊所/肾脏病理学会关于肾小球肾炎病理分类、诊断及报告共识[J].肾脏病与透析肾移植杂志,2016,25(3):261-268.
[3]周培清,温勇,曹智平,等.根除幽门螺杆菌对原发性肾小球肾炎患者蛋白尿的影响[J].临床和实验医学杂志,2016,15(18):1824-1827.
[4]Regna NL,Vieson MD,Luo XM,et al.Specific HDAC6 inhibition by ACY-738 reduces SLE pathogenesis in NZB/W mice[J].Clin Immunol,2016,1(162):58-73.
[5]Ryan J,Ma FY,Han Y,et al.Myeloid cell-mediated renal injury in rapidly progressive glomerulonephritis depends upon spleen tyrosine kinase[J].J Pathol,2016,238(1):10-20.
[6]陈敏广,叶晓华,梁海燕,等.原发性Ig A肾病患儿伴发急性肾损伤19例临床分析[J].中华儿科杂志,2016,54(8):610-613.
[7]唐余燕.CD4T细胞在Ig A肾病发病机制中的研究进展[J].国际泌尿系统杂志,2018,38(6):1050-1054.
[8]李宁,林源.贝那普利对系膜增生性肾小球肾炎大鼠血清和肾脏中MMP-9/TIMP-1的影响[J].临床和实验医学杂志,2018,17(19):2041-2043.
[9]朱春玲,胡春芳,王代宏,等.脉络宁注射液联合贝那普利治疗慢性肾小球肾炎的临床观察[J].中国药房,2016,27(20):2801-2803.
[10]吴玉员,柯喜梅,何昱,等.前列地尔联合贝那普利在糖尿病患者中的应用及对血糖波动的影响研究[J].中国生化药物杂志,2017,37(6):167-169.
[11]王念鸿,宫京梅,李英姿,等.贝那普利对早期糖尿病肾脏疾病足细胞损伤保护作用的实验研究[J].中国糖尿病杂志,2017,25(10):924-928.
[12]赵丽娜,胡浩琛,蒋杰,等.固定剂量氨氯地平联合贝那普利对比单药治疗高血压有效性和安全性的Meta分析[J].中国医药导报,2018,15(36):99-104.
[13]王学敏,王婧,赵讯,等.阿托伐他汀钙片联合贝那普利片治疗高血压肾病的临床研究[J].中国临床药理学杂志,2018,34(11):1306-1308.
[14]刘永建,齐洪娜,李佳,等.贝那普利联合曲美他嗪对老年冠心病并发慢性心力衰竭患者的炎性细胞因子及心功能的影响[J].广西医学,2016,38(5):644-647.
[15]陈建祥,徐宏,张敏.肾炎四味片对慢性肾小球肾炎血清IL-2、IL-6和T淋巴细胞亚群水平的影响[J].中国生化药物杂志,2016,41(1):129-131.
[2]曾彩虹.梅奥诊所/肾脏病理学会关于肾小球肾炎病理分类、诊断及报告共识[J].肾脏病与透析肾移植杂志,2016,25(3):261-268.
[3]周培清,温勇,曹智平,等.根除幽门螺杆菌对原发性肾小球肾炎患者蛋白尿的影响[J].临床和实验医学杂志,2016,15(18):1824-1827.
[4]Regna NL,Vieson MD,Luo XM,et al.Specific HDAC6 inhibition by ACY-738 reduces SLE pathogenesis in NZB/W mice[J].Clin Immunol,2016,1(162):58-73.
[5]Ryan J,Ma FY,Han Y,et al.Myeloid cell-mediated renal injury in rapidly progressive glomerulonephritis depends upon spleen tyrosine kinase[J].J Pathol,2016,238(1):10-20.
[6]陈敏广,叶晓华,梁海燕,等.原发性Ig A肾病患儿伴发急性肾损伤19例临床分析[J].中华儿科杂志,2016,54(8):610-613.
[7]唐余燕.CD4T细胞在Ig A肾病发病机制中的研究进展[J].国际泌尿系统杂志,2018,38(6):1050-1054.
[8]李宁,林源.贝那普利对系膜增生性肾小球肾炎大鼠血清和肾脏中MMP-9/TIMP-1的影响[J].临床和实验医学杂志,2018,17(19):2041-2043.
[9]朱春玲,胡春芳,王代宏,等.脉络宁注射液联合贝那普利治疗慢性肾小球肾炎的临床观察[J].中国药房,2016,27(20):2801-2803.
[10]吴玉员,柯喜梅,何昱,等.前列地尔联合贝那普利在糖尿病患者中的应用及对血糖波动的影响研究[J].中国生化药物杂志,2017,37(6):167-169.
[11]王念鸿,宫京梅,李英姿,等.贝那普利对早期糖尿病肾脏疾病足细胞损伤保护作用的实验研究[J].中国糖尿病杂志,2017,25(10):924-928.
[12]赵丽娜,胡浩琛,蒋杰,等.固定剂量氨氯地平联合贝那普利对比单药治疗高血压有效性和安全性的Meta分析[J].中国医药导报,2018,15(36):99-104.
[13]王学敏,王婧,赵讯,等.阿托伐他汀钙片联合贝那普利片治疗高血压肾病的临床研究[J].中国临床药理学杂志,2018,34(11):1306-1308.
[14]刘永建,齐洪娜,李佳,等.贝那普利联合曲美他嗪对老年冠心病并发慢性心力衰竭患者的炎性细胞因子及心功能的影响[J].广西医学,2016,38(5):644-647.
[15]陈建祥,徐宏,张敏.肾炎四味片对慢性肾小球肾炎血清IL-2、IL-6和T淋巴细胞亚群水平的影响[J].中国生化药物杂志,2016,41(1):129-131.