表面增强拉曼光谱法分析软饮料中的阿斯巴甜
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摘要
应用超声技术辅助预处理,以纳米银为活性基底,在溶液pH 5.0、纳米银与阿斯巴甜(APM)混合比例1∶1、混合温度30℃、加热时间6 min的条件下,利用表面增强拉曼光谱(SERS)结合化学计量法对纯品及软饮料中APM进行定量检测。结果表明:APM在0.5～100 mg/L范围内线性关系较好,其中水标准溶液的相关系数为0.993 3,检出限(LOD)为0.41 mg/L,苏打水、雪碧、可乐、芬达等标准溶液的相关系数为0.974 7～0.984 8,加标平均回收率为88.4%～121%,相对标准偏差小于7%。运用SERS检测软饮料中APM具有分析速度快、无损、环境污染小等优点,适用于食品中阿斯巴甜的定量分析,为食品添加剂的进一步表征和检测建立了依据,显示了SERS技术在化学物质检测领域具有巨大潜力。
A surface enhanced Raman spectroscopy(SERS) combined with chemometrics algorithms was established for the quantitative detection of aspartame(APM) in soft drinks based on the application of ultrasonography.The sample was detected using nano-silver as active substrate,with a nano-silver to APM ratio of 1 ∶1 at pH 5.0,a mixing temperature of 30 ℃ and a heating time of 6 min.A quantitative analysis mode was set up by using the monadic linear regression method to analyse SERS spectra.Results showed that the calibration curves were linear in the range of 0.5-100 mg/L.The correlation coefficient was 0.993 3 and the detection limit(LOD)was 0.41 mg/L for APM standard.The correlation coefficients of standard solutions in Soda water,Sprite,Coke and Fanta ranged from 0.974 7 to 0.984 8.Furthermore,the average recoveries ranged from 88.4% to 121% with relative standard deviations less than 7%.Detection of APM in soft drinks by SERS has the advantages of fast analysis,nondestructive and low environmental pollution,which indicates that SERS technology is suitable for quantitative analysis of APM in food and has great potential of applying in the determination of chemical substances.
A surface enhanced Raman spectroscopy(SERS) combined with chemometrics algorithms was established for the quantitative detection of aspartame(APM) in soft drinks based on the application of ultrasonography.The sample was detected using nano-silver as active substrate,with a nano-silver to APM ratio of 1 ∶1 at pH 5.0,a mixing temperature of 30 ℃ and a heating time of 6 min.A quantitative analysis mode was set up by using the monadic linear regression method to analyse SERS spectra.Results showed that the calibration curves were linear in the range of 0.5-100 mg/L.The correlation coefficient was 0.993 3 and the detection limit(LOD)was 0.41 mg/L for APM standard.The correlation coefficients of standard solutions in Soda water,Sprite,Coke and Fanta ranged from 0.974 7 to 0.984 8.Furthermore,the average recoveries ranged from 88.4% to 121% with relative standard deviations less than 7%.Detection of APM in soft drinks by SERS has the advantages of fast analysis,nondestructive and low environmental pollution,which indicates that SERS technology is suitable for quantitative analysis of APM in food and has great potential of applying in the determination of chemical substances.
引文
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[7] Huang Y H,Yu Q,Lin Q,Huang H X,Xie Y,Liang M.J.Instrum.Anal.(黄永辉,余清,林钦,黄红霞,谢勇,梁敏.分析测试学报),2011,30(8):877-882.
[8] Radulescu M C,Bucur B,Bucur M P,Radu G L.Sensors,2014,14(1):1028-1038.
[9] Deroco P B,Medeiros R A,Rocha-Filho R C,Fatibello-Filho O.Anal.Method,2015,7(5):2135.
[10] Liu D S.Application of Second-order Calibration Algorithms Coupled with Three-dimensional Fluorescence in Food Science.Changsha:Hunan University(刘迪思.食品分析中三维荧光二阶校正法的应用研究.长沙:湖南大学),2007.
[11] Tiu B D B,Pernites R B,Tiu S B,Advincula R C.Colloids Surf.A,2016,495:149-158.
[12] Zhang J,Yang H B,Xie J,Lu D,Chen L Y,Liu Y P,Sun C J.Mod.Prev.Med.(张静,杨鸿斌,谢娟,卢丹,陈璐莹,刘亚攀,孙成均.现代预防医学),2014,41(20):3768-3770.
[13] Ma J,Zhan M.RSC Adv.,2014,4(40):21060-21071.
[14] Peica N.J.Raman Spectrosc.,2009,40(12):2144-2154.
[15] Li C Y,Lai K Q,Zhang Y Y,Pei L,Huang Y Q.Acta Chim.Sin.(李春颖,赖克强,张源园,裴鹭,黄轶群.化学学报),2013,71:221-226.
[16] Giovannozzi A M,Rolle F,Sega M,Abete M C,Marchis D,Rossi A M.Food Chem.,2014,159:250-256.
[17] Wang L,Zhang A X,Chen X,Zheng X H,He G H.Food Res.Dev.(王乐,张爱霞,陈晞,郑新华,何桂华.食品研究与开发),2018,39(7):126-130.
[18] Ai Y,Liang P,Wu Y X,Dong Q M,Li J B,Bai Y,Ni D.Food Chem.,2018,241:427-433.