液相悬浮芯片技术在黑龙江省沙门菌血清分型中的应用
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摘要
目的探索液相悬浮芯片技术对黑龙江省食品风险监测的沙门菌分离株的血清分型效果及适用性。方法采用玻片凝集试验对分离的451株沙门菌进行分型,采用液相悬浮芯片技术——沙门菌血清型快速分型试剂盒(SSA)对其中256株沙门菌O抗原、H抗原、AT抗原基因进行分型鉴定。结果 249株分离株可以用SSA完整分型;液相悬浮芯片技术与传统方法血清分型的符合率超过96%。分离鉴定的44种血清型中有7种涉及到的O抗原或H抗原不在SSA检测范围内,其余37种能用SSA成功分型;利用SSA检测15株血清玻片凝集法不能分型的沙门菌,10株可成功分型。结论液相芯片技术能对大部分黑龙江省食品源沙门菌进行血清分型,与传统血清分型方法的一致性较高,且比传统方法具有高通量、高速度、准确性高的特点,可考虑常规用于沙门菌血清分型,特别是在应对食源性沙门菌感染暴发及食品风险监测时优先选用该方法进行血清型别的快速判定。
Objective To understand the effect and applicability of liquid suspension array Salmonella serotyping assay(SSA) in serotyping of Salmonella isolates from food risk surveillance in Heilongjiang province. Methods The traditional slide agglutination method was used for the serotyping of 451 Salmonella isolates, SSA kit was used to detect Salmonella O antigen, H antigen and AT antigen genes of 256 strains of these Salmonella isolates. Results A total of 249 Salmonella isolates could be completely serotyped by SSA kit. The consistent rate between SSA and slide agglutination method was above96%. Seven of 44 serotypes with O or H antigens were not detected by SSA kit and other 37 serotypes could be successfully serotyped by SSA kit. Fifteen Salmonella isolates could not be serotyped by slide agglutination method, but 10 of them could be successfully serotyped by SSA kit. Conclusion Salmonella serotyping assay kit can be used for serotyping of most foodborne Salmonella in Heilongjiang. It has high consistency with traditional serotyping method and has the characteristics of high throughput, high speed and high accuracy. SSA kit could be considered as routine serotyping of Salmonella especially in the response to outbreaks of food-borne Salmonella infection and food risk surveillance, it is preferred for rapid serotyping.
Objective To understand the effect and applicability of liquid suspension array Salmonella serotyping assay(SSA) in serotyping of Salmonella isolates from food risk surveillance in Heilongjiang province. Methods The traditional slide agglutination method was used for the serotyping of 451 Salmonella isolates, SSA kit was used to detect Salmonella O antigen, H antigen and AT antigen genes of 256 strains of these Salmonella isolates. Results A total of 249 Salmonella isolates could be completely serotyped by SSA kit. The consistent rate between SSA and slide agglutination method was above96%. Seven of 44 serotypes with O or H antigens were not detected by SSA kit and other 37 serotypes could be successfully serotyped by SSA kit. Fifteen Salmonella isolates could not be serotyped by slide agglutination method, but 10 of them could be successfully serotyped by SSA kit. Conclusion Salmonella serotyping assay kit can be used for serotyping of most foodborne Salmonella in Heilongjiang. It has high consistency with traditional serotyping method and has the characteristics of high throughput, high speed and high accuracy. SSA kit could be considered as routine serotyping of Salmonella especially in the response to outbreaks of food-borne Salmonella infection and food risk surveillance, it is preferred for rapid serotyping.
引文
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[2]张河战,辜清吾.沙门氏菌的分类、命名及中国沙门氏菌菌型分布[J].微生物学免疫学进展,2002,30(2):74-76.DOI:10.3969/j.issn.1005-5673.2002.02.018.Zhang HZ,Gu QW.Classification,nomenclature and distribution of Salmonella in China[J].Prog Microbiol Immunol,2002,30(2):74-76.DOI:10.3969/j.issn.1005-5673.2002.02.018.
[3]Herrera-León S,Ramiro R,Arroyo M,et al.Blind comparison of traditional serotyping with three multiplex PCRs for the identification of Salmonella serotypes[J].Res Microbiol,2007,158(2):122-127.DOI:10.1016/j.resmic.2006.09.009.
[4]Mashouf RY,Alikhani MY,Azadi S.Detection of Salmonella species isolated from clinical specimens by serotyping and polymerase chain reaction[J].Saudi Med J,2017,28(11):1751-1753.
[5]柯碧霞,何冬梅,谭海玲,等.基于微球液态阵列分子技术的沙门菌血清分型研究[J].中华流行病学杂志,2016,37(8):1137-1141.DOI:10.3760/cma.j.issn.0254-6450.2016.08.017.Ke BX,He DM,Tan HL,et al.Study on Salmonella serotyping by use of microsphere-based liquid array method[J].Chin JEpidemiol,2016,37(8):1137-1141.DOI:10.3760/cma.j.issn.0254-6450.2016.08.017.
[6]孙璐,王娟,黄秀梅,等.山东地区肉鸡屠宰环节沙门菌流行病学调查与毒力分析[J].中国家禽,2015,37(7):67-70.DOI:10.16372/j.issn.1004-6364.2015.07.017.Sun L,Wang J,Huang XM,et al.Epidemiological investigation and virulence analysis of Salmonella in broiler slaughtering links in Shandong province China poultry[J].Chin Poult,2015,37(7):67-70.DOI:10.16372/j.issn.1004-6364.2015.07.017.
[7]赵建梅,王娟,王君玮,等.山东地区生猪和家禽屠宰环节沙门菌血清型分布[J].中国食品卫生杂志,2015,27(5):503-508.DOI:10.13590/j.cjfh.2015.05.004.Zhao JM,Wang J,Wang JW,et al.Research on serotype distribution of Salmonella isolated from pig and poultry slaughter processes in Shandong province[J].Chin J Food Hyg,2015,27(5):503-508.DOI:10.13590/j.cjfh.2015.05.004.
[8]曾彪,王超,薛一凡,等.一起跨地区肠炎沙门氏菌食物中毒事件的流行病学调查与溯源[J].现代预防医学,2016,43(19):3479-3482.Zeng B,Wang C,Xue YF,et al.Investigation and tracing on a multi-district outbreak of food poisoning event caused by Salmonella Enteritidis[J].Mod Prev Med,2016,43(19):3479-3482.
[9]罗海波,何来英,叶伟杰,等.2004-2013年中国大陆食物中毒情况分析[J].中国食品卫生杂志,2015,27(1):45-49.DOI:10.13590/j.cjfh.2015.01.012.Luo HB,He LY,Ye WJ,et al.Analysis of the food poisoning in China from 2004 to 2013[J].Chin J Food Hyg,2015,27(1):45-49.DOI:10.13590/j.cjfh.2015.01.012.