摘要
目的开发闰年霉素的分离纯化工艺。方法采用二元液相分离系统,以反相填料为载体,对填料的类型和洗脱条件进行优化,得到适宜的操作条件。结果反相硅胶C18为最佳层析介质,洗脱剂为50%乙腈-酸水,上样量为10mg/m L填料。在此实验条件下所得产品纯度≥98.5%,纯化收率≥70%。结论此种闰年霉素的纯化方法简单易行,收率稳定,适于产业化应用。
Objective To develop the separation and purification processes of lipiarmycin. Methods Using a binary liquid chromatography system, the separation and purification processes of lipiarmycin were studied by optimizing the different types of chromatography fillers and elution conditions to obtain a suitable operating conditions. Results The results showed that the type of reverse phase silica gel C18 was selected as the best chromatography filler, 50% acetonitrile-H_2O was used as the eluent, and the amount of sample was 10 mg/mL filter. The purity of the final product was more than 98.5% and the yield of lipiarmycin was above 70% under the optimal conditions. Conclusion The method is simple and available for the commercial process.
引文
[1]Parenti F,Pagani H,Beretta G,et al.Lipiarmycin,anewantibiotic from actinoplanes.I.Description of the producer strain and fermentation studies[J].J Antibiot,1975,28(4):247-252.
[2]Adlman P J,Marks A M.Entrepreneu rial finance:Finance for small business[M].NewJersey:Prentice Hall,2002:456.
[3]Y-K·舒,C-K·王,Y-H·邱,等.18元环大环化合物及其类似物,CN:201210032395.4[P].
[4]Coronelli C,White R J,Lancini G C,et al.Lipiarmycin,anewantibiotic from actinoplanes.II.Isolation,chemical,biological and biochemical characterization[J].J Antibiot(Tokyo),1975,28(4):253-259.
[5]张长生,李苏梅,牛四文,等.六种台勾霉素类化合物及其制备方法和在制备抗菌药物中的应用,CN:201110104051[P].
[6]王欣荣,褚以文,翟龙飞,等.含有利福霉素和闰年霉素的组合物及其应用,CN:201711439795.8[P].