分子病理学诊断技术在食管癌患者诊断中的应用
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摘要
目的:探讨分子病理学诊断技术在食管癌患者中的诊断效果及与预后的相关性研究。方法:选取2016年5月-2017年5月本院食道癌患者64例,按照随机数字表法分为对照组与研究组,每组各32例。对照组采用常规传统的病理学诊断,研究组采用FISH检测法。对两组患者活检组织中病理结果、病例等级、检测的准确度、FISH的检测结果进行分析检测。结果:研究组患者病情程度显著高于对照组(P<0.05),检测结果表明食道癌与3号、17号染色体着丝粒异常拷贝有关(P<0.05)。结论:对于食管癌患者进行诊断时,采取更为敏感的FISH检测,显著提高检测的准确度,3号与17号染色体着丝点可以作为诊断食道癌的探针,分子病理学诊断技术可以进一步推广应用。
Objective:To investigate the effect of molecular pathology in the diagnosis of esophageal cancer and its correlation with prognosis.Method:Sixty-four patients with esophageal cancer in our hospital from May2016 to May 2017 were selected and divided into control group and research group according to random number table method,32 cases in each group.Conventional pathological diagnosis was used in the control group and FISH was used in the study group.The pathological results,case grade,accuracy of detection and FISH detection results in biopsy tissues of the two groups were analyzed and detected.Result:The degree of illness in the study group was significantly higher than that in the control group(P<0.05).FISH results showed that esophageal cancer could be associated with abnormal centromere copies of chromosomes 3 and 17(P<0.05).Conclusion:For the diagnosis of esophageal cancer patients,more sensitive FISH detection can significantly improve the accuracy of detection.Chromosome 3 and 17 centromeres can be used as probes for the diagnosis of esophageal cancer.Molecular pathological diagnosis technology can be further promoted and applied.
Objective:To investigate the effect of molecular pathology in the diagnosis of esophageal cancer and its correlation with prognosis.Method:Sixty-four patients with esophageal cancer in our hospital from May2016 to May 2017 were selected and divided into control group and research group according to random number table method,32 cases in each group.Conventional pathological diagnosis was used in the control group and FISH was used in the study group.The pathological results,case grade,accuracy of detection and FISH detection results in biopsy tissues of the two groups were analyzed and detected.Result:The degree of illness in the study group was significantly higher than that in the control group(P<0.05).FISH results showed that esophageal cancer could be associated with abnormal centromere copies of chromosomes 3 and 17(P<0.05).Conclusion:For the diagnosis of esophageal cancer patients,more sensitive FISH detection can significantly improve the accuracy of detection.Chromosome 3 and 17 centromeres can be used as probes for the diagnosis of esophageal cancer.Molecular pathological diagnosis technology can be further promoted and applied.
引文
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[16]李涛,赖春凤,郑敏莉,等.MTHFR基因多态性与客家人群食管癌易感性的关系[J].广东医学,2018,54(7):45-47.
[17]张冬云,陆蒂青,刘安丽,等.人食管鳞状细胞癌组织TIM-3表达临床意义[J].中华肿瘤防治杂志,2016,54(s2):35-36.
[18]刘建化,金浩生,田红霞,等.HBsAg阳性肝癌患者预后及病理因素与ALK基因表达的相关性研究[J].中国病理生理杂志,2016,32(5):841-846.
[19]程云杰,焦文鹏,王军,等.食管小细胞癌肿瘤标志物与淋巴结转移和预后的相关性分析[J].中华肿瘤防治杂志,2016,23(21):1426-1430.
[20]孙威,孙志强,汪建林,等.食管癌放疗肿瘤病理缓解程度及血管内皮生长因子变化与预后的相关性分析[J].中华放射医学与防护杂志,2018,38(7):517-523.
[2]Shi M,Feng J,Sun X,et al.Expression of lncRNA RP11-259P1.1 in esophageal squamous cell cancer and its correlation with clinical prognosis[J].Chinese Journal of Cancer Biotherapy,2017,73(3):32-33.
[3]李亭亭,潘德锋,朱红.血浆微小核糖核酸-1和微小核糖核酸-146b表达水平与急性ST段抬高型心肌梗死诊断及预后的相关性研究[J].中国循环杂志,2017,32(11)23-29.
[4]孙江涛,原翔,宋开放.ERCC1表达与晚期NSCLC吸烟患者预后的相关性研究[J].安徽医科大学学报,2017,52(7):1069-1072.
[5]刘宇宁,王小兵,王腾,等.巨噬细胞抑制因子-1与早期非小细胞肺癌诊断及预后相关性[J].中国肺癌杂志,2016,19(4):207-215.
[6]Abudoushalamu A,Madinyet N,Zhang H P,et al.The gene phenotype,protein expression of TP53 gene in Uygur’s esophageal carcinoma and its correlation with prognosis[J].China Medical Herald,2018,65(7):45-46.
[7]阎晓初,杨景.中华医学会病理学分会第二十一次学术会议曁第五届中国病理年会在昆明市召开[J].中华病理学杂志,2016,34(2):96.
[8]王福栋,宁振,王阿曼,等.胰腺导管内乳头状黏液肿瘤的分子病理学研究进展[J].中国普通外科杂志,2017,26(3):359-366.
[9]Xiao L U,Feng X S,Gao S G,et al.Expression of STAT3 and Nanog Gene in Esophageal Cancer and Its Correlation with the Clinicopathological Characteristics[J].Journal of Henan University of Science&Technology,2016,7(54):46-49.
[10]吴旻,陆艳荣,张瑾熔.AGGF1和HIF-1α及VEGF表达与食管鳞癌放疗预后相关性研究[J].中华肿瘤防治杂志,2016,23(17):1159-1164.
[11]李纯,刘静,关正元,等.TRAP1在食管癌中的表达及对预后的影响[J].临床与实验病理学杂志,2017,33(2):135-139.
[12]Yang Y,Wei S Z,DU Y X,et al.Expression of p53,Ki-67and PCNA in esophageal squamous cell carcinoma and correlation with radiotherapy effect and prognosis[J].Journal of Southeast University,2017,53(86)97-99.
[13]杜渐.胆管细胞癌的分子病理学研究进展[J].中国普通外科杂志,2017,26(2):241-245.
[14]郑唯强.乳腺癌分子病理诊断的机遇与挑战[J].临床与实验病理学杂志,2017,33(5):473-476.
[15]Inage T,Nakajima T,Fujiwara T,et al.Pathological diagnosis of pulmonary large cell neuroendocrine carcinoma by endobronchial ultrasound-guided transbronchial needle aspiration[J].Thoracic Cancer,2017,15(5 Suppl):131-133.
[16]李涛,赖春凤,郑敏莉,等.MTHFR基因多态性与客家人群食管癌易感性的关系[J].广东医学,2018,54(7):45-47.
[17]张冬云,陆蒂青,刘安丽,等.人食管鳞状细胞癌组织TIM-3表达临床意义[J].中华肿瘤防治杂志,2016,54(s2):35-36.
[18]刘建化,金浩生,田红霞,等.HBsAg阳性肝癌患者预后及病理因素与ALK基因表达的相关性研究[J].中国病理生理杂志,2016,32(5):841-846.
[19]程云杰,焦文鹏,王军,等.食管小细胞癌肿瘤标志物与淋巴结转移和预后的相关性分析[J].中华肿瘤防治杂志,2016,23(21):1426-1430.
[20]孙威,孙志强,汪建林,等.食管癌放疗肿瘤病理缓解程度及血管内皮生长因子变化与预后的相关性分析[J].中华放射医学与防护杂志,2018,38(7):517-523.