马铃薯晚疫病抗性基因分子标记检测及抗性评价
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摘要
马铃薯是重庆优势特色作物之一,但其安全生产受到晚疫病的严重威胁。马铃薯生产中种植抗病品种是防控马铃薯晚疫病最为经济有效和环境友好的途径。为了了解来自国内外不同种质的晚疫病抗性基因组成以及确定在重庆市具有晚疫病抗性的马铃薯品种(系),本研究以218份来自国内外的品种(系)为材料,进行了6个晚疫病抗性(R)基因分子标记检测,同时进行了田间晚疫病抗性评价及室内接种鉴定和筛选。研究结果显示,6个R基因的分子标记在供试材料中均有分布,但分子标记的组成不尽相同,主要分为4大类。第Ⅰ类含有具有广谱抗性基因的RB,晚疫病抗性评价表现为中抗以上;第Ⅲ类缺失R2 family基因标记,绝大部分表现为晚疫病敏感型;第Ⅱ类和第Ⅳ类中分别主要含有3个R基因(R2 Family+R3a+R3b)标记类型和4个R基因(R1+R2 Family+R3a+R3b)标记类型,这两类材料中表现出一定比例的晚疫病抗性水平,但第Ⅳ类中出现抗性表现的比例高于第Ⅱ类。结果说明含有RB基因标记贡献了较高的晚疫病抗性,缺失R2 famlily基因标记的材料可能不利于晚疫病抗性,利用这些基因标记辅助筛选有助于提高重庆地区晚疫病抗性育种效率。本研究评价了218份马铃薯材料6个重要R基因组成,并筛选出重庆地区表现抗性的多个材料,为新品种(系)的推广应用以及抗病育种选育提供了科学依据,同时为发掘新的抗病基因提供了遗传资源。
Potato is an important crop in mountainous areas of China,such as Chongqing. Late blight(LB)caused by Phytophthora infestans has been a major threat to sustainable potato production in China. Growing disease-resistant varieties is the most economical,effective and environmentally friendly way to control LB in potato production. In order to understand the composition of resistance(R)genes to late blight from different germplasms and to obtain potato varieties(lines)with resistance to LB in Chongqing,screening of R genes using molecular marker linked to LB and evaluation of LB resistance upon natural and artificial inoculations were carried out in 218 potato varieties(lines)from different regions. The results showed that six R genes were detected in the tested genotypes and the composition of the molecular markers could mainly be divided into four types,including Type Ⅰ,which was characterized by the presence of a broad-spectrum resistance gene RB;TypeⅢ,which was characterized by the absence of R2 family markers,most of which were susceptible to late blight;Type Ⅱ and Ⅳ,which were characterized by three R genes(R2 Family+R3 a+R3 b)and four R genes(R1+R2 Family+R3 a+R3 b)marker types,respectively,which showed a certain proportion of LB resistance in these two types. The proportion of resistance in type Ⅳ was higher than that in type Ⅱ. These results suggested that genotypes containing RB gene markers positively contributed to LB resistance. Screening of these gene markers will help to improve the breeding ef?ciency of LB resistance in Chongqing. By analyzing R genes compositions of and LB resistance tests in 218 potato genotypes,this study provided scienti?c basis for the future popularization and application of new varieties(lines)and resistance breeding in Chongqing,and also provided genetic resources for the discovery of new R genes.
Potato is an important crop in mountainous areas of China,such as Chongqing. Late blight(LB)caused by Phytophthora infestans has been a major threat to sustainable potato production in China. Growing disease-resistant varieties is the most economical,effective and environmentally friendly way to control LB in potato production. In order to understand the composition of resistance(R)genes to late blight from different germplasms and to obtain potato varieties(lines)with resistance to LB in Chongqing,screening of R genes using molecular marker linked to LB and evaluation of LB resistance upon natural and artificial inoculations were carried out in 218 potato varieties(lines)from different regions. The results showed that six R genes were detected in the tested genotypes and the composition of the molecular markers could mainly be divided into four types,including Type Ⅰ,which was characterized by the presence of a broad-spectrum resistance gene RB;TypeⅢ,which was characterized by the absence of R2 family markers,most of which were susceptible to late blight;Type Ⅱ and Ⅳ,which were characterized by three R genes(R2 Family+R3 a+R3 b)and four R genes(R1+R2 Family+R3 a+R3 b)marker types,respectively,which showed a certain proportion of LB resistance in these two types. The proportion of resistance in type Ⅳ was higher than that in type Ⅱ. These results suggested that genotypes containing RB gene markers positively contributed to LB resistance. Screening of these gene markers will help to improve the breeding ef?ciency of LB resistance in Chongqing. By analyzing R genes compositions of and LB resistance tests in 218 potato genotypes,this study provided scienti?c basis for the future popularization and application of new varieties(lines)and resistance breeding in Chongqing,and also provided genetic resources for the discovery of new R genes.
引文
[1]谢从华·马铃薯产业的现状与发展·华中农业大学学报:社会科学版,2012, 97(1):1-4Xie C H. The present situation and development of potato industry. Journal of Huazhong Agricultural University:Social Sciences Edition, 2012, 97(1):1-4
[2]金黎平,屈冬玉,谢开云,卞春松,段绍光·我国马铃薯种质资源和育种技术研究进展·种子,2003(5):98-100Jin L P, Qu D Y, Xie K Y, Bian C S, Duan S G. Advances ofpotato germplasm and breeding technology in China. Seed,2003(5):98-100
[3] Tian Y E, Yin J L, Sun J P, Li H P, Ma Y F, Wang Q H, Quan J L, Shan W X. Population genetic analysis of Phytophthora infestans in northwestern China. Plant Pathology, 2016, 65:17-25
[4] Li Y, van der Lee T, Zhu J H, Jin G H, Lan C Z, Zhu S X,Zhang R F, Liu B W, Zhao Z J, Kessel G, Huang S W, Jacobsen E. Population structure of Phytophthora infestans in Chinageographic clusters and presence of the EU genotype Blue 13.Plant Pathology, 2013,62(4):932-942
[5]黄振霖,欧建龙.重庆市马铃薯产业发展与主食化建议.南方农业,2017, 11(28):94-95Huang Z L, Ou J L. Potato industry development and suggestions on staple food in Chongqing. South China Agriculture,2017, 11(28):94-95
[6] Bradshaw J E, Ramsay G. Utilization of the commonwealth potato collection in potato breeding. Euphytica, 2005,146:9-19
[7] Ballvora A, Ercolano M R, Weiss J, Meksem K, Bormann C A, Oberhagemann P, Salamini F, Gebhardt C. The R1 gene for potato resistance to late blight(Phytophthora infestans)belongs to the leucine zipper/NBS/LRR class of plant resistance genes.The Plant Journal, 2002, 30:361-371
[8] Huang S, van der Vossen E A G, Kuang H, Vleeshouwers V G A, Zhang N, Borm T J A, van Eck H J, Baker B, Jacobsen E, Visser R G F. Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato-Plant Journal,2005,42:251-261
[9] Lokossou A A, Park T, van Arkel G, Arens M, Ruyter-Spira C,Morales J, Whisson S C, Birch P R J, Visser R G F, Jacobsen E, van der Vossen E A G-Exploiting knowledge of R/Avr genes to rapidly clone a new LZ-NBS-LRR family of late blight resistance genes from potato linkage groupⅣ. Molecular PlantMicrobe Interactions, 2009,22:630-641
[10] Li G, Huang S, Guo X,Li Y, Yang Y, Guo Z, Kuang H,Rietman H, Bergervoet M, Vleeshouwers V G A A, van der Vossen E,Qu D,Visser R G F,Jacobsen E,Vossen J H. Cloning and characterization of R3b; members of the R3 superfamily of late blight resistance genes show sequence and functional divergence. Molecular Plant-Microbe Interactions, 2011,24(10):1132-1142
[11] Jo K R, Arens M, Kim T Y, Jongsma M A, Visser R G F,Jacobsen E, Vossen J H. Mapping of the S.demissum late blight resistance gene R8 to a new locus on chromosome IX.Theoretical and Applied Genetics, 2011,123(8):1331-1340
[12] Li J, Lindqvist-Kreuze H Tian Z, Liu J, Song B, Landeo J,Portal L, Gastelo M, Frisancho J, Sanchez L, Meijer D, Xie C, Bonierbale M. Conditional QTL underlying resistance to late blight in a diploid potato population. Theoretical and Applied Genetics, 2012, 124:1339-1350
[13] Jiang R, Li J, Tian Z, Du J, Armstrong M, Baker K, Lim T Y, Vossen J, He H, Portal L, Zhou J, Bonierbale M, Hein I,Lindqvist-Kreuze H, Xie C. Potato late blight field resistance from QTL dPI09c is conferred by the NB-LRR.gene R8. Journal of Experimental Botany, 2018,69(7):1545-1555
[14] Song J, Bradeen J M, Naess S K, Raasch J A, Wielgus S M,Haberlach G T,Liu J, Kuang H, Austin-Phillips S, BuellC R, Helgeson J P, Jiang J. Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight. Proceedings of the National Academy of Sciences,USA, 2003, 100:9128-9133
[15]Colton L M,Groza H I,Wielgus S M,Jiang J. Marker-assisted selection for the broad-spectrum potato late blight resistance conferred by gene RB derived from a wild potato species. Crop Science,2006, 46(2):589-594
[16]Tiwari J K,Siddappa S,Singh B P,Kaushik S K,Chakrabarti S K, Bhardwaj V, Chandel P Molecular markers for late blight resistance breeding of potato:an update. Plant Breeding, 2013,132(3):237-245
[17]白磊,郭华春·马铃薯多抗亲本的分子标记辅助筛查·分子植物育种,2017, 14(1):200-212Bai L, Guo H C. The molecular markers assisted screening of multiple resistance potato parents. Molecular Plant Breeding,2017, 14(1):200-212
[18]徐建飞,金黎平·马铃薯遗传育种研究:现状与展望·中国农业科学,2017, 50(6):990-1015Xu J F, Jin L P. Advances and perspectives in research of potato genetics and breeding. Scientia Agricultura Sinica, 2017,50(6):990-1015
[19] Kim H J, Lee H R, Jo K R, Mortazavian S M M, Huigen D J, Evenhuis B, Kessel G, Visser R G F, Jacobsen E, Vossen J H. Broad spectrum late blight resistance in potato differential set plants MaR8 and MaR9 is conferred by multiple stacked R genes. Theoretical and Applied Genetics, 2012, 124:923-935
[20]李文娟,FORBES Gregory A,谢开云·马铃薯晚疫病发病程度田间观察记录标准的探讨·中国马铃薯,2012(4):238-246Li W J, Forbes G A, Xie K Y. Observations on the standardization of field assessment of potato late blight severity. China Potato Journal, 2012(4):238-246
[21] Du J, Verzaux E, Chaparro-Garcia A, Bijsterbosch G, Keizer L C P, Zhou J, Liebrand T W H, Xie C, Govers F, Robatzek S,van der Vossen E A G, Jacobsen E, Visser R G F, Kamoun S,Vleeshouwers V G. Elicitin recognition confers enhanced resistance to Phytophthora infestans in potato. Nature Plants, 2015, 1(4):15034
[22]王金萍,刘永伟,孙果忠,王海波·抗茎腐病分子标记在159份玉米自交系中的验证及实用性评价·植物遗传资源学报,2017, 18(4):754-762Wang J P, Liu Y W, Sun G Z, Wang H B Evaluation and validation of molecular markers associated with stalk rot resistance in 159maize inbred lines.Journal of Plant Genetic Resources, 2017. 18(4):754-762
[23]向小姣,张建,郑天清,徐建龙·应用分子标记技术改良京作1号的稻瘟病扰抗性·植物遗传资源学报,2016, 17(4):773-780Xiang X J, Zhang J, Zheng T Q, Xu J L. Improving blast resistance of Jinggengl using molecular marker technique. Journal of Plant Genetic Resources, 2016, 17(4):773-780
[24] Zhu S, Li Y, Vossen J H, Visser R G, Jacobsen E. Functional stacking of three resistance genes against Phytophthora infestans in potato. Transgenic Research, 2012,21:89-99
[25] Haesaert G, Vossen J H, Custers R, De Loose M, Haverkort A, Heremans B, Hutten R, Kessel G, Landschoot S,Droogenbroeck B, Visser R G F, Gheysen G-Transformation of the potato variety Desiree with single or multiple resistance genes increases resistance to late blight under field conditions.Crop Protection, 2015,77:163-175
[26] Vleeshouwers V G,Rietman H,Krenek P,Champouret N,Young C, Oh S K, Wang M, Bouwmeester K, Vosman B,Visser R G F, Jacobsen E, Govers F, Kamoun S, van der Vossen E. Effector genomics accelerates discovery and functional profiling of potato disease resistance and phytophthora infestans avirulence genes, PLoS One, 2008, 3(8):e2875
[27] Jupe F,Witek K,Verweij W,Sliwka J,Pritchard L,Etherington G J, Maclean D, Cock P J, Leggett R M, Bryan G J, Cardle L, Hein I, Jones J D. Resistance gene enrichment sequencing(RenSeq)enables reannotation of the NB-LRR gene family from sequenced plant genomes and rapid mapping of resistance loci in segregating populations. The Plant Journal,2013,76:530-544
[28] Witek K, Jupe F, Witek A I, Baker D, Clark M D, Jones J D.Accelerated cloning of a potato late blight-resistance gene using RenSeq and SMRT sequencing. Nature Biotechnology, 2016,34(6):656-660
[29] Steuemagel B, Periyannan S K, Hema ndez-Pinzo n I, Witek K,Rouse M N, Yu G, Hatta A, Ayliffe M, Bariana H, Jones J D G,Lagudah E S, Wulff B B H·Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture. Nature Biotechnology, 2016,34:652-655
[30] Chen X, Lewandowska D, Armstrong M R, Baker K, Lim T Y,Bayer M,Harrower B.McLean K,Jupe F,Witek K,Lees A K,Jones J D, Bryan G J, Hein I. Identification and rapid mappingof a gene conferring broad-spectrum late blight resistance in the diploid potato species solanum verrucosum through DNA capture technologies. Theoretical and Applied Genetics, 2018,10:1-11
[31]毕朝位·重庆地区致病疫霉phytophthora infestans(Mont.)de Bary生物学特性研究·重庆:西南农业大学,2002Bi C W. Biological Characteristics of Phtophthorn infostsms(Mont.)de Bary in Chongqing. Chongqing:Southwest Agricultural University, 2002
[32]黄振霖,张国臣,杨水英,李建华,青玲,欧建龙,赵雨佳,孙现超,严秋艳·重庆马铃薯晚疫病菌群体遗传结构研究·西南大学学报:自然科学版,2010, 32(6):17-22Huang Z L, Zhang G C, Yang S Y, Li J H, Qing L, Ou J L, Zhao Y J, Sun X C, Yan Q Y. Population genetic structure of late blight(Phytophthora infestans)of potato in Chongqing. Journal of Southwest University:Natural Science Edition, 2010, 32(6):17-22
[33]石纹豪,黄振霖,杨水英,洪枫,李振轮,欧建龙,赵雨佳·重庆马铃薯致病疫霉(phytophthora infestans)的SSR基因型分析·西南大学学报:自然科学版,2014, 36(12):36-41Shi W H, Huang Z L, Yang S Y, Hong F, Li Z L, Ou J L, Zhao Y J. SSR genotypic analysis of Phytophthora infestans on potato plants in Chongqing. Journal of Southwest University:Natural Science Edition, 2014, 36(12):36-41
[34]赵青·川渝地区马铃薯晚疫病菌群体表型和遗传变异的分析·兰州:甘肃农业大学,2015Zhao Q. Analysis on phenotypic and genotypic variations of Phytophthora infestans in Sichuan-Chongqing region. Lanzhou:Ganshu Agricultural University, 2015
[2]金黎平,屈冬玉,谢开云,卞春松,段绍光·我国马铃薯种质资源和育种技术研究进展·种子,2003(5):98-100Jin L P, Qu D Y, Xie K Y, Bian C S, Duan S G. Advances ofpotato germplasm and breeding technology in China. Seed,2003(5):98-100
[3] Tian Y E, Yin J L, Sun J P, Li H P, Ma Y F, Wang Q H, Quan J L, Shan W X. Population genetic analysis of Phytophthora infestans in northwestern China. Plant Pathology, 2016, 65:17-25
[4] Li Y, van der Lee T, Zhu J H, Jin G H, Lan C Z, Zhu S X,Zhang R F, Liu B W, Zhao Z J, Kessel G, Huang S W, Jacobsen E. Population structure of Phytophthora infestans in Chinageographic clusters and presence of the EU genotype Blue 13.Plant Pathology, 2013,62(4):932-942
[5]黄振霖,欧建龙.重庆市马铃薯产业发展与主食化建议.南方农业,2017, 11(28):94-95Huang Z L, Ou J L. Potato industry development and suggestions on staple food in Chongqing. South China Agriculture,2017, 11(28):94-95
[6] Bradshaw J E, Ramsay G. Utilization of the commonwealth potato collection in potato breeding. Euphytica, 2005,146:9-19
[7] Ballvora A, Ercolano M R, Weiss J, Meksem K, Bormann C A, Oberhagemann P, Salamini F, Gebhardt C. The R1 gene for potato resistance to late blight(Phytophthora infestans)belongs to the leucine zipper/NBS/LRR class of plant resistance genes.The Plant Journal, 2002, 30:361-371
[8] Huang S, van der Vossen E A G, Kuang H, Vleeshouwers V G A, Zhang N, Borm T J A, van Eck H J, Baker B, Jacobsen E, Visser R G F. Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato-Plant Journal,2005,42:251-261
[9] Lokossou A A, Park T, van Arkel G, Arens M, Ruyter-Spira C,Morales J, Whisson S C, Birch P R J, Visser R G F, Jacobsen E, van der Vossen E A G-Exploiting knowledge of R/Avr genes to rapidly clone a new LZ-NBS-LRR family of late blight resistance genes from potato linkage groupⅣ. Molecular PlantMicrobe Interactions, 2009,22:630-641
[10] Li G, Huang S, Guo X,Li Y, Yang Y, Guo Z, Kuang H,Rietman H, Bergervoet M, Vleeshouwers V G A A, van der Vossen E,Qu D,Visser R G F,Jacobsen E,Vossen J H. Cloning and characterization of R3b; members of the R3 superfamily of late blight resistance genes show sequence and functional divergence. Molecular Plant-Microbe Interactions, 2011,24(10):1132-1142
[11] Jo K R, Arens M, Kim T Y, Jongsma M A, Visser R G F,Jacobsen E, Vossen J H. Mapping of the S.demissum late blight resistance gene R8 to a new locus on chromosome IX.Theoretical and Applied Genetics, 2011,123(8):1331-1340
[12] Li J, Lindqvist-Kreuze H Tian Z, Liu J, Song B, Landeo J,Portal L, Gastelo M, Frisancho J, Sanchez L, Meijer D, Xie C, Bonierbale M. Conditional QTL underlying resistance to late blight in a diploid potato population. Theoretical and Applied Genetics, 2012, 124:1339-1350
[13] Jiang R, Li J, Tian Z, Du J, Armstrong M, Baker K, Lim T Y, Vossen J, He H, Portal L, Zhou J, Bonierbale M, Hein I,Lindqvist-Kreuze H, Xie C. Potato late blight field resistance from QTL dPI09c is conferred by the NB-LRR.gene R8. Journal of Experimental Botany, 2018,69(7):1545-1555
[14] Song J, Bradeen J M, Naess S K, Raasch J A, Wielgus S M,Haberlach G T,Liu J, Kuang H, Austin-Phillips S, BuellC R, Helgeson J P, Jiang J. Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight. Proceedings of the National Academy of Sciences,USA, 2003, 100:9128-9133
[15]Colton L M,Groza H I,Wielgus S M,Jiang J. Marker-assisted selection for the broad-spectrum potato late blight resistance conferred by gene RB derived from a wild potato species. Crop Science,2006, 46(2):589-594
[16]Tiwari J K,Siddappa S,Singh B P,Kaushik S K,Chakrabarti S K, Bhardwaj V, Chandel P Molecular markers for late blight resistance breeding of potato:an update. Plant Breeding, 2013,132(3):237-245
[17]白磊,郭华春·马铃薯多抗亲本的分子标记辅助筛查·分子植物育种,2017, 14(1):200-212Bai L, Guo H C. The molecular markers assisted screening of multiple resistance potato parents. Molecular Plant Breeding,2017, 14(1):200-212
[18]徐建飞,金黎平·马铃薯遗传育种研究:现状与展望·中国农业科学,2017, 50(6):990-1015Xu J F, Jin L P. Advances and perspectives in research of potato genetics and breeding. Scientia Agricultura Sinica, 2017,50(6):990-1015
[19] Kim H J, Lee H R, Jo K R, Mortazavian S M M, Huigen D J, Evenhuis B, Kessel G, Visser R G F, Jacobsen E, Vossen J H. Broad spectrum late blight resistance in potato differential set plants MaR8 and MaR9 is conferred by multiple stacked R genes. Theoretical and Applied Genetics, 2012, 124:923-935
[20]李文娟,FORBES Gregory A,谢开云·马铃薯晚疫病发病程度田间观察记录标准的探讨·中国马铃薯,2012(4):238-246Li W J, Forbes G A, Xie K Y. Observations on the standardization of field assessment of potato late blight severity. China Potato Journal, 2012(4):238-246
[21] Du J, Verzaux E, Chaparro-Garcia A, Bijsterbosch G, Keizer L C P, Zhou J, Liebrand T W H, Xie C, Govers F, Robatzek S,van der Vossen E A G, Jacobsen E, Visser R G F, Kamoun S,Vleeshouwers V G. Elicitin recognition confers enhanced resistance to Phytophthora infestans in potato. Nature Plants, 2015, 1(4):15034
[22]王金萍,刘永伟,孙果忠,王海波·抗茎腐病分子标记在159份玉米自交系中的验证及实用性评价·植物遗传资源学报,2017, 18(4):754-762Wang J P, Liu Y W, Sun G Z, Wang H B Evaluation and validation of molecular markers associated with stalk rot resistance in 159maize inbred lines.Journal of Plant Genetic Resources, 2017. 18(4):754-762
[23]向小姣,张建,郑天清,徐建龙·应用分子标记技术改良京作1号的稻瘟病扰抗性·植物遗传资源学报,2016, 17(4):773-780Xiang X J, Zhang J, Zheng T Q, Xu J L. Improving blast resistance of Jinggengl using molecular marker technique. Journal of Plant Genetic Resources, 2016, 17(4):773-780
[24] Zhu S, Li Y, Vossen J H, Visser R G, Jacobsen E. Functional stacking of three resistance genes against Phytophthora infestans in potato. Transgenic Research, 2012,21:89-99
[25] Haesaert G, Vossen J H, Custers R, De Loose M, Haverkort A, Heremans B, Hutten R, Kessel G, Landschoot S,Droogenbroeck B, Visser R G F, Gheysen G-Transformation of the potato variety Desiree with single or multiple resistance genes increases resistance to late blight under field conditions.Crop Protection, 2015,77:163-175
[26] Vleeshouwers V G,Rietman H,Krenek P,Champouret N,Young C, Oh S K, Wang M, Bouwmeester K, Vosman B,Visser R G F, Jacobsen E, Govers F, Kamoun S, van der Vossen E. Effector genomics accelerates discovery and functional profiling of potato disease resistance and phytophthora infestans avirulence genes, PLoS One, 2008, 3(8):e2875
[27] Jupe F,Witek K,Verweij W,Sliwka J,Pritchard L,Etherington G J, Maclean D, Cock P J, Leggett R M, Bryan G J, Cardle L, Hein I, Jones J D. Resistance gene enrichment sequencing(RenSeq)enables reannotation of the NB-LRR gene family from sequenced plant genomes and rapid mapping of resistance loci in segregating populations. The Plant Journal,2013,76:530-544
[28] Witek K, Jupe F, Witek A I, Baker D, Clark M D, Jones J D.Accelerated cloning of a potato late blight-resistance gene using RenSeq and SMRT sequencing. Nature Biotechnology, 2016,34(6):656-660
[29] Steuemagel B, Periyannan S K, Hema ndez-Pinzo n I, Witek K,Rouse M N, Yu G, Hatta A, Ayliffe M, Bariana H, Jones J D G,Lagudah E S, Wulff B B H·Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture. Nature Biotechnology, 2016,34:652-655
[30] Chen X, Lewandowska D, Armstrong M R, Baker K, Lim T Y,Bayer M,Harrower B.McLean K,Jupe F,Witek K,Lees A K,Jones J D, Bryan G J, Hein I. Identification and rapid mappingof a gene conferring broad-spectrum late blight resistance in the diploid potato species solanum verrucosum through DNA capture technologies. Theoretical and Applied Genetics, 2018,10:1-11
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