基于HPLC-LTQ/Orbitrap MS法的四味脾胃舒颗粒化学成分研究
|
摘要
[目的]应用高效液相色谱-线性离子阱/静电场轨道阱高分辨质谱(HPLC-LTQ/Orbitrap MS)技术鉴定四味脾胃舒颗粒中的化学成分,为四味脾胃舒颗粒的深入研究开发提供更多科学依据。[方法]通过查阅文献,收集整理四味脾胃舒颗粒处方中各味药材(茯苓、鸡屎藤、山楂、陈皮)及其同科属植物各类化学成分数据。采用HPLC-LTQ/Or-bitrap MS法采集数据后,对各色谱峰的质谱图高分辨一级和二级质谱数据进行解析,与文献数据进行对比,对各色谱峰进行结构推测和确认。[结果]从四味脾胃舒颗粒样品中共分析出30个成分,鉴定了26个化合物,分别为黄酮类成分(橙皮素、柚皮素、刺芒柄花素)、苷类成分(Cyanidin 3-galactoside、野樱苷、芹菜苷)、酸类成分(去氢土莫酸、没食子酸)、生物碱类成分(刺桐碱、3-formylindole)。[结论]实验所建立的HPLC-LTQ/Orbitrap MS法适合于鉴定中药复方制剂四味脾胃舒颗粒主要化学成分,在一定程度上揭示了四味脾胃舒颗粒的药效物质物质基础。
[Objective] Analysis and identification chemical constituents in Siwei Piweishu Granule by HPLC-LTQ/Orbitrap MS. To provides scientific basis for further research and development of Siwei Piweishu Granule. [Methods] Through literature review,to collect the data of all kinds of chemical constituents in the prescription of Siwei Piweishu Granule. Data is collected by HPLC-LTQ/Orbitrap MS,High resolution MS and MS2 spectra of mass spectrogram of chromatographic peaks are analysised. Compare with the literature database.The structure of each chromatographic peak was calculated and confirmed. [Results] Twenty-six were identified from Siwei Piweishu Granule. The main constituents are Flavonoids(hesperetin,naringenin,formononetin);glycosides(cyanidin 3-galactoside,prunasin,apiin);acids(dehydrotumulosic acid,gallic acid);Alkaloids(hypaphorine,3-formylindole). [Conclusion] In this paper,HPLC-LTQ/Orbitrap MS was suitable to determine and analyze the mainly chemical composition of Siwei Piweishu Granule. To a certain extent,the pharmacodynamic material basis of Siwei Piweishu Granule was revealed by this research.
[Objective] Analysis and identification chemical constituents in Siwei Piweishu Granule by HPLC-LTQ/Orbitrap MS. To provides scientific basis for further research and development of Siwei Piweishu Granule. [Methods] Through literature review,to collect the data of all kinds of chemical constituents in the prescription of Siwei Piweishu Granule. Data is collected by HPLC-LTQ/Orbitrap MS,High resolution MS and MS2 spectra of mass spectrogram of chromatographic peaks are analysised. Compare with the literature database.The structure of each chromatographic peak was calculated and confirmed. [Results] Twenty-six were identified from Siwei Piweishu Granule. The main constituents are Flavonoids(hesperetin,naringenin,formononetin);glycosides(cyanidin 3-galactoside,prunasin,apiin);acids(dehydrotumulosic acid,gallic acid);Alkaloids(hypaphorine,3-formylindole). [Conclusion] In this paper,HPLC-LTQ/Orbitrap MS was suitable to determine and analyze the mainly chemical composition of Siwei Piweishu Granule. To a certain extent,the pharmacodynamic material basis of Siwei Piweishu Granule was revealed by this research.
引文
[1]张栋健,李薇,何庆文,等.UHPLC-Q-TOF-MS分析枳壳炮制前后成分变化[J].中国中药杂志,2016,41(11):2070-2080.
[2]程巧鸳,王笑笑.UPLC-MS/MS法分析3种蜂蜜及其蜜源花中的特征黄酮类组分[J].药物分析杂志,2017(6):994-1000.
[3]刘亚丽,魏韶锋,宋永贵,等.UPLC/Q-TOF-MS/MS法分析丰城鸡血藤中刺芒柄花素在大鼠肠道菌群中的代谢[J].中国新药杂志,2015(23):2715-2723.
[4]刘贤青,张红艳.HPLC-Q-TOF/MS分析脐橙果实中的类黄酮[J].植物科学学报,2014,32(6):638-644.
[5]丁健桦,王兴祥,张慧,等.芹菜素的电喷雾萃取电离串联质谱[J].高等学校化学学报,2011,32(8):1714-1719.
[6]Li DN,Meng XJ,Li B.Profiling of anthocyanins from blueberries produced in China using HPLC-DAD-MS and exploratory analysis by principal component analysis[J].Journal of Food Composition and Analysis,2016,47(6):1-7.
[7]于澎,张虹.HPLC-ESI-MS法同时测定虎杖提取物中的17种成分[J].沈阳药科大学学报,2011,191(12):963-968.
[8]胡杨,刘春明,胡蕴梅,等.川芎化学成分的高效液相色谱-电喷雾质谱研究[J].时珍国医国药,2012,23(8):1868-1869.
[9]尹权微,李家春,秦建平,等.UPLC/Q-TOF-MS快速分析桂枝茯苓胶囊的入血成分[J].中国实验方剂学杂志,2016,22(21):83-86.
[10]Marius BP,Simona D1,Floricua R,et al.Comparative HPLC-DAD-ESI(+)MS Fingerprint and Quantification ofPhenolic and Flavonoid Composition of Aqueous Leaf Extracts of Cornusmas and Crataegus monogyna,in Relation to Their Cardiotonic Potential[J].2014,42(1):9-18.
[11]刘亚男,郭锦明,王跃飞,等.波长切换高效液相色谱法测定山茱萸中马钱苷和没食子酸[J].天津中医药,2009,26(4):152-154.
[12]李健康,张敏,刘校妃,等.UPLC-MS/MS快速测定茯苓中去氢土莫酸和茯苓酸的含量[J].中国实验方剂学杂志,2017,23(4):85-88.
[13]Hai JZ.Profiling Analysis of the Seeds of Vaccaria segetalis(Necr.)Gracke by HPLC-ESI-MS[J].Advanced Materials Research,2012,396-398(5):96-98.
[14]袁帅,姚胜军,耿昱,等.HPLC-ESI-MS/MS识别蓝莓提取物中的花青素和黄酮醇[J].化学学报,2009,67(4):318-322.
[15]Qiao X,Zhang YT,Ye M,et al.Analysis of chemical constituents and taxonomic similarity of Salvia species in China using LC/MS[J].Planta Medica,2009,75(15):1613-1617.
[16]黄爱华,迟玉广,曾元儿,等.枳实黄酮对功能性消化不良大鼠胃肠动力的影响[J].中药新药与临床药理,2012,23(6):612-615.
[17]朱瑞超,逯彬,罗蓉,等.桑叶7种黄酮类成分同时测定及其抑制α-糖苷酶活性的研究[J].天津中医药,2018,35(4):302-306.
[18]李晶,孔维松,刘欣,等.山楂中一个具有抗菌性的黄酮类新化合物[J].天然产物研究与开发,2018,31(6):964-967.
[2]程巧鸳,王笑笑.UPLC-MS/MS法分析3种蜂蜜及其蜜源花中的特征黄酮类组分[J].药物分析杂志,2017(6):994-1000.
[3]刘亚丽,魏韶锋,宋永贵,等.UPLC/Q-TOF-MS/MS法分析丰城鸡血藤中刺芒柄花素在大鼠肠道菌群中的代谢[J].中国新药杂志,2015(23):2715-2723.
[4]刘贤青,张红艳.HPLC-Q-TOF/MS分析脐橙果实中的类黄酮[J].植物科学学报,2014,32(6):638-644.
[5]丁健桦,王兴祥,张慧,等.芹菜素的电喷雾萃取电离串联质谱[J].高等学校化学学报,2011,32(8):1714-1719.
[6]Li DN,Meng XJ,Li B.Profiling of anthocyanins from blueberries produced in China using HPLC-DAD-MS and exploratory analysis by principal component analysis[J].Journal of Food Composition and Analysis,2016,47(6):1-7.
[7]于澎,张虹.HPLC-ESI-MS法同时测定虎杖提取物中的17种成分[J].沈阳药科大学学报,2011,191(12):963-968.
[8]胡杨,刘春明,胡蕴梅,等.川芎化学成分的高效液相色谱-电喷雾质谱研究[J].时珍国医国药,2012,23(8):1868-1869.
[9]尹权微,李家春,秦建平,等.UPLC/Q-TOF-MS快速分析桂枝茯苓胶囊的入血成分[J].中国实验方剂学杂志,2016,22(21):83-86.
[10]Marius BP,Simona D1,Floricua R,et al.Comparative HPLC-DAD-ESI(+)MS Fingerprint and Quantification ofPhenolic and Flavonoid Composition of Aqueous Leaf Extracts of Cornusmas and Crataegus monogyna,in Relation to Their Cardiotonic Potential[J].2014,42(1):9-18.
[11]刘亚男,郭锦明,王跃飞,等.波长切换高效液相色谱法测定山茱萸中马钱苷和没食子酸[J].天津中医药,2009,26(4):152-154.
[12]李健康,张敏,刘校妃,等.UPLC-MS/MS快速测定茯苓中去氢土莫酸和茯苓酸的含量[J].中国实验方剂学杂志,2017,23(4):85-88.
[13]Hai JZ.Profiling Analysis of the Seeds of Vaccaria segetalis(Necr.)Gracke by HPLC-ESI-MS[J].Advanced Materials Research,2012,396-398(5):96-98.
[14]袁帅,姚胜军,耿昱,等.HPLC-ESI-MS/MS识别蓝莓提取物中的花青素和黄酮醇[J].化学学报,2009,67(4):318-322.
[15]Qiao X,Zhang YT,Ye M,et al.Analysis of chemical constituents and taxonomic similarity of Salvia species in China using LC/MS[J].Planta Medica,2009,75(15):1613-1617.
[16]黄爱华,迟玉广,曾元儿,等.枳实黄酮对功能性消化不良大鼠胃肠动力的影响[J].中药新药与临床药理,2012,23(6):612-615.
[17]朱瑞超,逯彬,罗蓉,等.桑叶7种黄酮类成分同时测定及其抑制α-糖苷酶活性的研究[J].天津中医药,2018,35(4):302-306.
[18]李晶,孔维松,刘欣,等.山楂中一个具有抗菌性的黄酮类新化合物[J].天然产物研究与开发,2018,31(6):964-967.