杜氏盐藻(Dunaliella salina)对重金属铜胁迫的生理响应
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摘要
将不同质量浓度的CuCl_2溶液添加到培养基中,研究重金属铜对杜氏盐藻(Dunaliella salina)胁迫的生理响应.分别用不同质量浓度的CuCl_2溶液培养盐藻,检测盐藻的光合色素、可溶性多糖、蛋白质、SOD及MDA质量浓度的变化.结果显示,铜可抑制盐藻细胞的生长,且呈剂量-效应关系,72 h的EC50为9. 55 mg/L.随着铜质量浓度的增加,各质量浓度组盐藻细胞内叶绿素a、b、总叶绿素、类胡萝卜素、多糖和蛋白质质量浓度均显著性降低(P <0. 05或P <0. 01).铜可导致盐藻细胞氧化损伤,随着铜质量浓度的升高,SOD活力先升高后降低,MDA质量浓度升高.表明铜可以抑制盐藻细胞内光合色素、多糖和蛋白质的合成.盐藻对铜胁迫的响应机制可能是通过抗氧化防御系统.
To study the physiological response of heavy metal copper stress on Dunaliella salina. Dunaliella salina was cultured with different concentrations of CuCl_2 solution. The photosynthetic pigments,soluble polysaccharides,protein,SOD and MDA contents were determined. The results showed that copper could inhibit the growth of D. salina cells in a dosedependent manner. The EC50 at 72 h was 9. 55 mg/L. With the increase of copper concentration,the contents of chlorophyll a,b,total chlorophyll,carotenoid,polysaccharides and protein significantly decreased( P < 0. 05 or P < 0. 01). Copper could cause oxidative damage of D. salina cells. In addition,with the increase of copper concentration,SOD activity increased first and then decreased,and MDA content increased. Copper could inhibit the synthesis of photosynthetic pigments,polysaccharides and proteins in D. salina. The mechanism of D. salina in response to copper may be the antioxidant defense system.
To study the physiological response of heavy metal copper stress on Dunaliella salina. Dunaliella salina was cultured with different concentrations of CuCl_2 solution. The photosynthetic pigments,soluble polysaccharides,protein,SOD and MDA contents were determined. The results showed that copper could inhibit the growth of D. salina cells in a dosedependent manner. The EC50 at 72 h was 9. 55 mg/L. With the increase of copper concentration,the contents of chlorophyll a,b,total chlorophyll,carotenoid,polysaccharides and protein significantly decreased( P < 0. 05 or P < 0. 01). Copper could cause oxidative damage of D. salina cells. In addition,with the increase of copper concentration,SOD activity increased first and then decreased,and MDA content increased. Copper could inhibit the synthesis of photosynthetic pigments,polysaccharides and proteins in D. salina. The mechanism of D. salina in response to copper may be the antioxidant defense system.
引文
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[2] TAN S Y,PRAVEENA S M,ABIDIN E Z,et al. A review of heavy metals in indoor dust and its human health-risk implications[J]. Rev Environ Health,2016,31(4):447-456.
[3] PENG C,MA Y,DING Y,et al. Influence of Speciation of Thorium on Toxic Effects to Green Algae Chlorella pyrenoidosa[J]. Int J Mol Sci,2017,18(4). pii:E795
[4] DAO L H,BEARDALL J. Effects of lead on growth,photosynthetic characteristics and production of reactive oxygen species of two freshwater green algae[J]. Chemosphere,2016,147:420-429.
[5] CHEN Z,SONG S,WEN Y,et al. Toxicity of Cu(II)to the green alga Chlorella vulgaris:a perspective of photosynthesis and oxidant stress[J]. Environ Sci Pollut Res Int,2016,23(18):17910-17918.
[6] MARANGONI L F B,MARQUES J A,DUARTE G A S,et al.Copper effects on biomarkers associated with photosynthesis,oxidative status and calcification in the Brazilian coral Mussismilia harttii(Scleractinia,Mussidae)[J]. Mar Environ Res,2017,130:248-257.
[7] WANG H,EBENEZER V,KI J S. Photosynthetic and biochemical responses of the freshwater green algae Closterium ehrenbergii Meneghini(Conjugatophyceae)exposed to the metal coppers and its implication for toxicity testing[J]. J Microbiol,2018,56(6):426-434.
[8] FAWAZ E G,SALAM D A,KAMAREDDINE L. Evaluation of copper toxicity using site specific algae and water chemistry:Field validation of laboratory bioassays[J]. Ecotoxicol Environ Saf,2018,155:59-65.
[9] HOSSEINI T A,SHARIATI M. Dunaliella biotechnology:methods and applications[J]. J Appl Microbiol,2009,107(1):14-35.
[10] LAMERS P P,JANSSEN M,DE VOS R C,et al. Exploring and exploiting carotenoid accumulation in Dunaliella salina for cell-factory applications[J]. Trends Biotechnol,2008,26(11):631-638.
[11] SAHA S K,KAZIPET N,MURRAY P. The Carotenogenic Dunaliella salina CCAP 19/20 Produces Enhanced Levels of Carotenoid under Specific Nutrients Limitation[J]. Biomed Res Int,2018:7532897.
[12] SINGH P,BARANWAL M,REDDY S M. Antioxidant and cytotoxic activity of carotenes produced by Dunaliella salina under stress[J]. Pharm Biol,2016,54(10):2269-2275.
[13] HONG L,LIU J L,MIDOUN S Z,et al. Transcriptome sequencing and annotation of the halophytic microalga Dunaliella salina[J]. J Zhejiang Univ,2017,18(10):833-844.
[14] GONG F,WANG J,LI J. Isolation and characterization of peroxiredoxin 1 gene of Dunaliella salina[J]. Gene,2017,635:39-45.
[15] NIKOOKAR K,MORADSHAHI A,HOSSEINI L. Physiological responses of Dunaliella salina and Dunaliella tertiolecta to copper toxicity[J]. Biomol Eng,2005,22(4):141-146.
[16]杨晓玲,郭金耀.微量元素铜、钼对盐藻生长与物质积累的影响[J].江苏农业科学,2013,41(2):192-194.
[17]凌娜,仲星,李红秀,等.杜氏盐藻对不同价态铁的吸收机制研究[J].中国海洋药物,2015,34(3):29-34.
[18] XU Y,IBRAHIM I M,HARVEY P J. The influence of photoperiod and light intensity on the growth and photosynthesis of Dunaliella salina(chlorophyta)CCAP 19/30[J]. Plant Physiol Biochem,2016,106:305-315.
[19] LING N,SUN Q Y,MAO Y X,et al. Effect of iron on the growth and nutritional quality of Chlorella pyrenoidosa[J].Chin J Mar Drugs,2014,33(4):45-49.
[20]师玥.青岛大扁藻光合作用过程对重金属Cu2+急性毒性胁迫的响应研究[D].青岛:中国海洋大学,2012.
[21] ARUN N,VIDYALAXMI,SINGH D P. Chromium(VI)induced oxidative stress in halotolerant alga Dunaliella salina and D. tertiolecta isolated from sambhar salt lake of Rajasthan(India)[J]. Cell Mol Biol(Noisy-le-grand),2014,60(5):90-96.
[22] QIAN L,QI S,CAO F,et al. Toxic effects of boscalid on the growth,photosynthesis,antioxidant system and metabolism of Chlorella vulgaris[J]. Environ Pollut,2018,242(Pt A):171-181.