金属离子对微生物蛋白酶活性的影响及机理
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摘要
微生物蛋白酶广泛应用于人们的生产生活中,无论是工业上蛋白酶制剂的生产,还是食品发酵产业中,提高微生物蛋白酶活性一直属于热门话题。研究表明添加一定量的金属离子对于蛋白酶活性的提高具有显著作用。本文主要介绍了金属离子对微生物蛋白酶活性的影响,并对国内、外关于金属离子影响微生物蛋白酶活性的机理研究进行论述,旨在为工业生产及食品发酵相关产业提供一定参考依据。
Microbial protease is widely applied in people's life, both in industrial production of zymin and food fermentation industry. Improving microbial protease activity has always been a hot topic. The study showed metal ions played a significant role in improving the protease activity. In this paper, the effect and mechanism of metal ions on the activity of microbial protease were summarized, aiming to provide references for industrial production and food fermentation industry.
Microbial protease is widely applied in people's life, both in industrial production of zymin and food fermentation industry. Improving microbial protease activity has always been a hot topic. The study showed metal ions played a significant role in improving the protease activity. In this paper, the effect and mechanism of metal ions on the activity of microbial protease were summarized, aiming to provide references for industrial production and food fermentation industry.
引文
[1]BEG K B,SAHAI V,GUPTA R.Statistical media optimization and alkaline protease production from Bacillus mojavensis in a biore-actor[J].Process Biochem,2003,39(2):203-209.
[2]SABOTI C J,KOS J.Microbial and fungal protease inhibitors current and potential applications[J].Appl Microbiol Biotechnol,2012,93(4):1351-1375.
[3]YUN-HAE L,MIHOKO T,RISA H,et al.Aspergillus oryzae strains with a large deletion of the aflatoxin biosynthetic homologous gene cluster differentiated by chromosomal breakage[J].Appl Microbiol Biotechnol,2006,72(2):339-345.
[4]BIESEBEKE R,BIEZEN N,RECORD E,et al.Branching mutants of Aspergillus oryzae with improved amylase and protease production on solid substrates[J].Appl Microbiol Biotechnol,2005,69(1):44-50.
[5]MACHIDA M,ASAI K,SANO M.Genome sequencing and analysis of Aspergillus oryzae[J].Nature,2005,438(7071):1157-1161.
[6]WANG J,XU A,WAN Y,et al.Purification and characterization of a new metallo-neutral protease for beer brewing from Bacillus amyloliquefaciens,SYB-001[J].Applied Biochemistry&Biotechnology,2013,170(8):2021-2033.
[7]OKUDA M,SUMITOMO N,TAKIMURA Y,et al.A new subtilisin family:nucleotide and deduced amino acid sequences of new high-molecular-mass alkaline proteases from Bacillus spp.[J].Extremophiles,2004,8(3):229-235.
[8]陈鸿图.高产中性蛋白酶菌株选育及产酶特性研究[D].广州:华南理工大学,2012.
[9]LI C,XU D,ZHAO M,et al.Production optimization,purification,and characterization of a novel acid protease from a fusant by Aspergillus oryzae,and Aspergillus niger[J].European Food Research&Technology,2014,238(6):905-917.
[10]NILANTHA-LAKSHMAN P,TOYOKAWA Y,TOYAMA H,et al.Purification and characterisation of two extracellular acid proteinases from Monascus pilosus[J].Food Chem,2010,121(4):1216-1224.
[11]YIN L J,HSU T H,JIANG S T.Characterization of acidic protease from Aspergillus niger BCRC32720[J].Agric Food Chem,2013,61(3):662-666.
[12]方春玉,周健,邓静,等.泸型大曲黑曲霉产酸性蛋白酶条件的优化及其酶学性质的研究[J].食品与发酵科技,2011,47(2):13-17.
[13]张秀江,胡虹,范国歌,等.酸性蛋白酶高产菌株选育及酶学性质的研究[J].河南科学,2012,30(3):327-332.
[14]LEE S K,HWANG J Y,CHOI S H,et al.Purification and characterization of Aspergillus oryzae LK-101 salt-tolerant acid protease isolated from soybean paste[J].Food Science&Biotechnology,2010,19(2):327-334.
[15]ALAM S I,DUBE S,REDDY G S N,et al.Purification and characterisation of extracellular protease produced by Clostridium sp.from Schirmacher oasis,Antarctica[J].Enzyme&Microbial Technology,2005,36(6):824-831.
[16]IDA N,TOKUSHIGE M.Assignment of catalytically essential cysteine residues in aspartase by selective chemical modification with N-(7-dimethylamino-4-methylcoumarynyl)maleimide[J].Journal of Biochemistry,1985,98(3):793-797.
[17]KARSTEN W E,GATES R B,VIOLA R E.Kinetic studies of Laspartase from Escherichia coli:substrate activation[J].Biochemistry,1986,25(6):1299-1303.
[18]王习文,詹怀宇,何为.金属离子对漆酶活性的影响[J].中华纸业,2003,24(6):33-35.
[19]SAWADA J.Studies on the acid-protease of Paecilomyces varioti bainier TPR-220[J].Agric Biol Chem,1964,28(12):348-355.
[20]曹治云.黑曲霉产酸性蛋白酶催化机制和稳定剂研究[D].福州:福建师范大学,2005.
[21]竺礼斌,曲道峰,施春伟,等.高产蛋白酶枯草芽孢杆菌JNB001产酶条件的优化[J].中国畜牧兽医,2015,42(2):414-419.
[22]刘源,李诗雯,彭铭烨,等.不同生化因子对米曲霉蛋白酶系活力的影响[J].中国酿造,2015,34(12):55-59.
[23]WANG J,XU A,WAN Y,et al.Purification and characterization of a new metallo-neutral protease for beer brewing from Bacillus amyloliquefaciens,SYB-001[J].Applied Biochemistry&Biotechnology,2013,170(8):2021-2033.
[24]MA X,LIU Y,LI Q,et al.Expression,purification and identification of a thermolysin-like protease,neutral protease I,from Aspergillus oryzae,with the Pichia pastoris,expression system[J].Protein Expression&Purification,2016,128:52-59.
[25]PRIHANTO A A,JAZIRI A A,PERWIRA I YPurification and characterization of neutral protease from Bacillus Substilis Ubt7 isolated from Terasi,Indonesian fermented fish[J].Biosciences Biotechnology Research Asia,2016,13(3):1409-1413.
[26]ROSSMANN M G,BLOW D M.The detection of sub-units within the crystallographic asymmetric unit[J].Acta Cryst,1962,15(1):24-31.
[27]YASUNOBU K T,MCCONN J.Bacillus subtilis,neutral protease[J].Methods in Enzymology,1970,19:569-575.
[28]郑穗平,郭勇,潘力.酶学[M].北京:科学出版社,2009:61-62.
[29]IFTIKHAR T M,NIAZ M A,ZIA M,et al.Production potential of locally isolated strain of Fusarium solani(MBL 24)for extracellular lipases[J].Pak J Bot,2012,44(1):393-397.
[30]HOLMQUIST B,VALLEE B L.Metal substitutions and inhibition of thermolysin:Spectra of the cobalt enzyme[J].Journal of Biological Chemistry,1974,249(14):4601-4607.
[31]LARSEN K S,AULD D S.Characterization of an inhibitory metal binding site in carboxypeptidase A[J].Biochemistry,1991,30(10):2613-2618
[32]胡皆汉,郑学仿.枯草杆菌中性蛋白酶与无机金属化合物相互作用的核磁共振谱[J].科学通报,2000,45(9):932-935.
[33]李红,王惠,尼秀媚,等.普鲁兰短梗霉N2-3菌株碱性蛋白酶的纯化及性质研究[J].安徽农业科学,2012,40(32):15588-15592.
[34]高瑞昌,刘向东,陆文婷,等.嗜盐古生菌Halogranum rubrum RO2-11胞外蛋白酶酶学特性研究[J].现代食品科技,2015,31(2):32-36.
[35]徐亚男,史学伟,童军茂,等.产蛋白酶酵母菌的选育及酶学特性[J].现代食品科技,2015,31(10):146-150.
[36]JAOUADI B,ABDELMALEK B,FODIL D,et al Purification and characterization of a thermostable keratinolytic serine alkaline proteinase from Streptomyces sp.strain AB1 with high stability in organic solvents[J].Bioresource Technology,2010,101(21):8361-8369.
[37]TOUIOUI S B,JAOUADI N Z,BOUDJELLA H,et al.Purification and biochemical characterization of two detergent-stable serine alkaline proteases from Streptomyces sp.strain AH4[J].World Journal of Microbiology&Biotechnology,2015,31(7):1079-1092.
[38]ANJUM S,MUKHTAR H,NAWAZ A,et al.Stability and activity profile of alkaline protease produced from Bacillus subtilis[J].Pakistan Journal of Botany,2015,47(2):771-777.
[39]波莱纳,麦凯布.工业酶:结构、功能与应用[M].北京:科学出版社,2010:160-165.
[40]ADINARAYNA K,ELLAIAH P,PRASAD D SPurification and characterization of thermostable serine alkaline protease from a newly isolated Bacillus subtilis PE-11[J].AAPS Pharmsci Tech,2003,4(4):1-9.
[41]NASCIMENTO W C A D,MARTINS M L L.Production and properties of an extracellular protease from thermophilic Bacillus sp.[J].Brazilian Journal of Microbiology,2004,35(35):91-96.
[42]ANANDAN D,MARMER W N,DUDLEY R LIsolation,characterization and optimization of culture parameters for production of an alkaline protease isolated from Aspergillus tamarii[J].Journal of Industrial Microbiology&Biotechnology,2007,34(5):339-347.
[43]吕明生,王淑军,李华钟,等.海洋细菌Pseudoalteromonas flavipulchra HH407产低温碱性蛋白酶的发酵条件和酶学性质研究[J].食品科学,2010,31(21):298-303.
[44]YANG J,SHIH II,TZENG Y,et al.Production and purification of protease from a Bacillus subtilis that can deproteinize crustacean wastes[J].Enzyme Microb Technol,2000,26(5/6):406-413.
[45]MICHELS P C,CLARK D S.Pressure-enhanced activity and stability of a hyperthermophilic protease from a Deep-Sea methanogen[J].Appl Environ Microbiol,1997,63(21):3985-3991.
[46]LEE S,JANG D J.Progressive rearrangement of subtilisin Carlsberg into orderly and inflexible conformation with Ca2+binding[J].Biophys J,2001,81(5):2972-2988.
[47]WANG W.Lyophilization and development of solid protein pharmaceuticals[J].Intl Jour Pharm,2000,203(1/2):1-60.
[48]BECKER N T,BOTT R R,BOWDEN S L,et al Stabilization of enzymes:EP,US7157516[P].2007.
[49]KUMAR C G,TIWARI M P,JANY K D.Novel alkaline serine proteases from alkalophilic Bacillus spp.:Purification and some properties[J].Proc Biochem,1999,34(5):441-449.
[2]SABOTI C J,KOS J.Microbial and fungal protease inhibitors current and potential applications[J].Appl Microbiol Biotechnol,2012,93(4):1351-1375.
[3]YUN-HAE L,MIHOKO T,RISA H,et al.Aspergillus oryzae strains with a large deletion of the aflatoxin biosynthetic homologous gene cluster differentiated by chromosomal breakage[J].Appl Microbiol Biotechnol,2006,72(2):339-345.
[4]BIESEBEKE R,BIEZEN N,RECORD E,et al.Branching mutants of Aspergillus oryzae with improved amylase and protease production on solid substrates[J].Appl Microbiol Biotechnol,2005,69(1):44-50.
[5]MACHIDA M,ASAI K,SANO M.Genome sequencing and analysis of Aspergillus oryzae[J].Nature,2005,438(7071):1157-1161.
[6]WANG J,XU A,WAN Y,et al.Purification and characterization of a new metallo-neutral protease for beer brewing from Bacillus amyloliquefaciens,SYB-001[J].Applied Biochemistry&Biotechnology,2013,170(8):2021-2033.
[7]OKUDA M,SUMITOMO N,TAKIMURA Y,et al.A new subtilisin family:nucleotide and deduced amino acid sequences of new high-molecular-mass alkaline proteases from Bacillus spp.[J].Extremophiles,2004,8(3):229-235.
[8]陈鸿图.高产中性蛋白酶菌株选育及产酶特性研究[D].广州:华南理工大学,2012.
[9]LI C,XU D,ZHAO M,et al.Production optimization,purification,and characterization of a novel acid protease from a fusant by Aspergillus oryzae,and Aspergillus niger[J].European Food Research&Technology,2014,238(6):905-917.
[10]NILANTHA-LAKSHMAN P,TOYOKAWA Y,TOYAMA H,et al.Purification and characterisation of two extracellular acid proteinases from Monascus pilosus[J].Food Chem,2010,121(4):1216-1224.
[11]YIN L J,HSU T H,JIANG S T.Characterization of acidic protease from Aspergillus niger BCRC32720[J].Agric Food Chem,2013,61(3):662-666.
[12]方春玉,周健,邓静,等.泸型大曲黑曲霉产酸性蛋白酶条件的优化及其酶学性质的研究[J].食品与发酵科技,2011,47(2):13-17.
[13]张秀江,胡虹,范国歌,等.酸性蛋白酶高产菌株选育及酶学性质的研究[J].河南科学,2012,30(3):327-332.
[14]LEE S K,HWANG J Y,CHOI S H,et al.Purification and characterization of Aspergillus oryzae LK-101 salt-tolerant acid protease isolated from soybean paste[J].Food Science&Biotechnology,2010,19(2):327-334.
[15]ALAM S I,DUBE S,REDDY G S N,et al.Purification and characterisation of extracellular protease produced by Clostridium sp.from Schirmacher oasis,Antarctica[J].Enzyme&Microbial Technology,2005,36(6):824-831.
[16]IDA N,TOKUSHIGE M.Assignment of catalytically essential cysteine residues in aspartase by selective chemical modification with N-(7-dimethylamino-4-methylcoumarynyl)maleimide[J].Journal of Biochemistry,1985,98(3):793-797.
[17]KARSTEN W E,GATES R B,VIOLA R E.Kinetic studies of Laspartase from Escherichia coli:substrate activation[J].Biochemistry,1986,25(6):1299-1303.
[18]王习文,詹怀宇,何为.金属离子对漆酶活性的影响[J].中华纸业,2003,24(6):33-35.
[19]SAWADA J.Studies on the acid-protease of Paecilomyces varioti bainier TPR-220[J].Agric Biol Chem,1964,28(12):348-355.
[20]曹治云.黑曲霉产酸性蛋白酶催化机制和稳定剂研究[D].福州:福建师范大学,2005.
[21]竺礼斌,曲道峰,施春伟,等.高产蛋白酶枯草芽孢杆菌JNB001产酶条件的优化[J].中国畜牧兽医,2015,42(2):414-419.
[22]刘源,李诗雯,彭铭烨,等.不同生化因子对米曲霉蛋白酶系活力的影响[J].中国酿造,2015,34(12):55-59.
[23]WANG J,XU A,WAN Y,et al.Purification and characterization of a new metallo-neutral protease for beer brewing from Bacillus amyloliquefaciens,SYB-001[J].Applied Biochemistry&Biotechnology,2013,170(8):2021-2033.
[24]MA X,LIU Y,LI Q,et al.Expression,purification and identification of a thermolysin-like protease,neutral protease I,from Aspergillus oryzae,with the Pichia pastoris,expression system[J].Protein Expression&Purification,2016,128:52-59.
[25]PRIHANTO A A,JAZIRI A A,PERWIRA I YPurification and characterization of neutral protease from Bacillus Substilis Ubt7 isolated from Terasi,Indonesian fermented fish[J].Biosciences Biotechnology Research Asia,2016,13(3):1409-1413.
[26]ROSSMANN M G,BLOW D M.The detection of sub-units within the crystallographic asymmetric unit[J].Acta Cryst,1962,15(1):24-31.
[27]YASUNOBU K T,MCCONN J.Bacillus subtilis,neutral protease[J].Methods in Enzymology,1970,19:569-575.
[28]郑穗平,郭勇,潘力.酶学[M].北京:科学出版社,2009:61-62.
[29]IFTIKHAR T M,NIAZ M A,ZIA M,et al.Production potential of locally isolated strain of Fusarium solani(MBL 24)for extracellular lipases[J].Pak J Bot,2012,44(1):393-397.
[30]HOLMQUIST B,VALLEE B L.Metal substitutions and inhibition of thermolysin:Spectra of the cobalt enzyme[J].Journal of Biological Chemistry,1974,249(14):4601-4607.
[31]LARSEN K S,AULD D S.Characterization of an inhibitory metal binding site in carboxypeptidase A[J].Biochemistry,1991,30(10):2613-2618
[32]胡皆汉,郑学仿.枯草杆菌中性蛋白酶与无机金属化合物相互作用的核磁共振谱[J].科学通报,2000,45(9):932-935.
[33]李红,王惠,尼秀媚,等.普鲁兰短梗霉N2-3菌株碱性蛋白酶的纯化及性质研究[J].安徽农业科学,2012,40(32):15588-15592.
[34]高瑞昌,刘向东,陆文婷,等.嗜盐古生菌Halogranum rubrum RO2-11胞外蛋白酶酶学特性研究[J].现代食品科技,2015,31(2):32-36.
[35]徐亚男,史学伟,童军茂,等.产蛋白酶酵母菌的选育及酶学特性[J].现代食品科技,2015,31(10):146-150.
[36]JAOUADI B,ABDELMALEK B,FODIL D,et al Purification and characterization of a thermostable keratinolytic serine alkaline proteinase from Streptomyces sp.strain AB1 with high stability in organic solvents[J].Bioresource Technology,2010,101(21):8361-8369.
[37]TOUIOUI S B,JAOUADI N Z,BOUDJELLA H,et al.Purification and biochemical characterization of two detergent-stable serine alkaline proteases from Streptomyces sp.strain AH4[J].World Journal of Microbiology&Biotechnology,2015,31(7):1079-1092.
[38]ANJUM S,MUKHTAR H,NAWAZ A,et al.Stability and activity profile of alkaline protease produced from Bacillus subtilis[J].Pakistan Journal of Botany,2015,47(2):771-777.
[39]波莱纳,麦凯布.工业酶:结构、功能与应用[M].北京:科学出版社,2010:160-165.
[40]ADINARAYNA K,ELLAIAH P,PRASAD D SPurification and characterization of thermostable serine alkaline protease from a newly isolated Bacillus subtilis PE-11[J].AAPS Pharmsci Tech,2003,4(4):1-9.
[41]NASCIMENTO W C A D,MARTINS M L L.Production and properties of an extracellular protease from thermophilic Bacillus sp.[J].Brazilian Journal of Microbiology,2004,35(35):91-96.
[42]ANANDAN D,MARMER W N,DUDLEY R LIsolation,characterization and optimization of culture parameters for production of an alkaline protease isolated from Aspergillus tamarii[J].Journal of Industrial Microbiology&Biotechnology,2007,34(5):339-347.
[43]吕明生,王淑军,李华钟,等.海洋细菌Pseudoalteromonas flavipulchra HH407产低温碱性蛋白酶的发酵条件和酶学性质研究[J].食品科学,2010,31(21):298-303.
[44]YANG J,SHIH II,TZENG Y,et al.Production and purification of protease from a Bacillus subtilis that can deproteinize crustacean wastes[J].Enzyme Microb Technol,2000,26(5/6):406-413.
[45]MICHELS P C,CLARK D S.Pressure-enhanced activity and stability of a hyperthermophilic protease from a Deep-Sea methanogen[J].Appl Environ Microbiol,1997,63(21):3985-3991.
[46]LEE S,JANG D J.Progressive rearrangement of subtilisin Carlsberg into orderly and inflexible conformation with Ca2+binding[J].Biophys J,2001,81(5):2972-2988.
[47]WANG W.Lyophilization and development of solid protein pharmaceuticals[J].Intl Jour Pharm,2000,203(1/2):1-60.
[48]BECKER N T,BOTT R R,BOWDEN S L,et al Stabilization of enzymes:EP,US7157516[P].2007.
[49]KUMAR C G,TIWARI M P,JANY K D.Novel alkaline serine proteases from alkalophilic Bacillus spp.:Purification and some properties[J].Proc Biochem,1999,34(5):441-449.
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