共代谢策略强化微生物燃料电池降解甲硝唑的研究
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摘要
本实验以醋酸钠(2mM)作为共代谢基质,以甲硝唑为目标污染物,运行四个产电周期。实验结果表明:燃料电池体系分别加入35mg/L、15 mg/L、5 mg/L甲硝唑后运行至第四个周期,燃料电池峰值电压分别下降了88%、80%、7%;功率密度分别下降了94%、71%、3%。当共基质醋酸钠比例较低时,循环伏安特性曲线氧化还原峰消失,甲硝唑去除效率降低,细胞破损较为严重,细胞质溶出发生明显的质壁分离现象;而当共基质醋酸钠浓度充足时,氧化还原峰并没有显著的变化,甲硝唑去除效率升高,细胞的完整性较好,处于健康状态。实验结果表明醋酸钠作为共代谢基质促进了细胞色素对电子的传递作用,加快了体系的电能转化,削弱了甲硝唑对微生物的抑制作用,使燃料电池运行更加稳定。
We employed metronidazole as the target refractory pollutant and acetate as the co-substrate in this work. As a result, MFC system was run to the futher fourth cycle after adding 35 mg/L, 15 mg/L and 5 mg/L metronidazole respectively.The peak voltage decreased by 88 %, 80 % and 7 % respectively, and the power density decreased by 94 %, 71 % and 3 % respectively. When the proportion of sodium acetate was insufficiency, the redox peak of CV curve was disappear, and the removal efficiency of metronidazole was reduced, the cell was damaged seriously, and obvious plasmolysis occurred. However, When the proportion of sodium acetate was sufficiency, CV curve was no significant change,and metronidazole removal efficiency increased, cell structure was integrated and in a healthy state. These results show that sodium acetate as a co-substrate promotes the electron transfer of cytochrome, accelerates the energy conversion of the MFC system, weakens the inhibitory effect of metronidazole on microorganisms, and makes the system more stable.
We employed metronidazole as the target refractory pollutant and acetate as the co-substrate in this work. As a result, MFC system was run to the futher fourth cycle after adding 35 mg/L, 15 mg/L and 5 mg/L metronidazole respectively.The peak voltage decreased by 88 %, 80 % and 7 % respectively, and the power density decreased by 94 %, 71 % and 3 % respectively. When the proportion of sodium acetate was insufficiency, the redox peak of CV curve was disappear, and the removal efficiency of metronidazole was reduced, the cell was damaged seriously, and obvious plasmolysis occurred. However, When the proportion of sodium acetate was sufficiency, CV curve was no significant change,and metronidazole removal efficiency increased, cell structure was integrated and in a healthy state. These results show that sodium acetate as a co-substrate promotes the electron transfer of cytochrome, accelerates the energy conversion of the MFC system, weakens the inhibitory effect of metronidazole on microorganisms, and makes the system more stable.
引文
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[15]Luo W,Zhao Y,Ding H,et al.Co-metabolic degradation of bensulfuron-methyl in laboratory conditions[J].Journal of Hazardous Materials,2008,158(1):208-214.
[2]Benotti M J,Brownawell B J.Microbial degradation of pharmaceuticals in estuarine and coastal seawater[J].Environmental Pollution,2009,157(3):994-1002.
[3]Uwe Schr?der.Anodic electron transfer mechanisms in microbial fuel cells and their energy efficiency[J].Physical Chemistry Chemical Physics Pccp,2007,9(21):2619-2629.
[4]Jang J K,Kan J,Bretschger O,et al.Electricity Generation by Microbial Fuel Cell Using Microorganisms as Catalyst in Cathode[J].Journal of Microbiology and Biotechnology,2013,23(12):1765.
[5]Ingerslev F,Halling-S?rensen B.Biodegradability of metronidazole,olaquindox,and tylosin and formation of tylosin degradation products in aerobic soil-manure slurries[J].Ecotoxicology&Environmental Safety,2001,48(3):311-320.
[6]Logan B B.Production of Electricity from Acetate or Butyrate Using a Single-Chamber Microbial Fuel Cell[J].Environmental science&technology,2005,39(2):658-662.
[7]Min B,Logan B E.Continuous electricity generation from domestic wastewater and organic substrates in a flat plate microbial fuel cell[J].Environmental Science&Technology,2004,38(21):5809-5814.
[8]Santos T C,Silva M A,Morgado L,et al.Diving into the redox properties of Geobacter sulfurreducens cytochromes:a model for extracellular electron transfer.Dalton transactions(Cambridge,England:2003,44(20):9335-9344.
[9]Liang S,Rosso K M,Clarke T A,et al.Molecular Underpinnings of Fe(III)Oxide Reduction by Shewanella Oneidensis MR-1[J].Frontiers in Microbiology,2012,3(50):50.
[10]Song H,Guo W,Liu M,et al.Performance of microbial fuel cells on removal of metronidazole.[J].Water Science&Technology A Journal of the International Association on Water Pollution Research,2013,68(12):2599.
[11]Xiong H,Dong S,Zhang J,et al.Roles of an easily biodegradable co-substrate in enhancing tetracycline treatment in an intimately coupled photocatalytic-biological reactor[J].Water Research,2018,136:75.
[12]Kaihovaara P,H??knikanne J,Uusioukari M,et al.Flavodoxin-dependent pyruvate oxidation,acetate production and metronidazole reduction by Helicobacter pylori[J].Journal of Antimicrobial Chemotherapy,1998,41(2):171-7.
[13]Duan L,Jiang W,Song Y,et al.The characteristics of extracellular polymeric substances and soluble microbial products in moving bed biofilm reactor-membrane bioreactor[J].Bioresource Technology,2013,148(Complete):436-442.
[14]Kim S,Hwang J,Chung J,et al.Enhancing trichloroethylene degradation using non-aromatic compounds as growth substrates[J].Journal of Hazardous Materials,2014,275(2):99-106.
[15]Luo W,Zhao Y,Ding H,et al.Co-metabolic degradation of bensulfuron-methyl in laboratory conditions[J].Journal of Hazardous Materials,2008,158(1):208-214.