欧洲冬青对低温胁迫的生理响应及抗寒性评价
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摘要
以欧洲冬青(Ilex aquifolium)原种,2个欧洲冬青园艺杂交种[‘长叶阿尔塔’(Ilex×altaclerensis‘Belgica Aurea’]、[‘金心阿尔塔’(Ilex×altaclerensis ‘Lawsoniana’)]和2个欧洲冬青品种[‘布利奥特夫人’(Ilex aquifolium.‘Madame Briot’]、[‘小宝石’(Ilex aquifolium‘Little Gem’)]的2年生扦插苗为试验材料,进行低温胁迫处理,观测形态特征,测定不同低温处理下的相对电导率(REC)、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、可溶性糖含量和叶绿素总含量等生理指标,并用主成分分析法和隶属函数法对5种植物的抗寒性进行综合评价。结果表明,5种植物的抗寒性大小依次为:‘长叶阿尔塔’>‘小宝石’>欧洲冬青原种>‘布利奥特夫人’>‘金心阿尔塔’。
In this paper,two years old seedlings of Ilex aquifolium,two hybrids of Ilex aquifolium( Ilex × altaclerensis‘Belgica Aurea',Ilex × altaclerensis‘Lawsoniana') and two varieties of Ilex aquifolium( Ilex aquifolium.‘Madame Briot',Ilex aquifolium‘Little Gem') were placed in different low temperature conditions,the morphological characteristics,relative conductivity,the activity of superoxide( SOD),the level of malondialdehyde( MDA),the contents of soluble sugar and chlorophyll were studied. Comprehensive evaluation of cold resistance on five kinds of plants was conducted by principal component analysis and subordinate function analysis.The results revealed that cold resistance of five kinds of plants from strong to weak was Ilex × altaclerensis ‘Belgica Aurea'> Ilex aquifolium‘Little Gem'> Ilex aquifolium > Ilex aquifolium. ‘Madame Briot'> Ilex × altaclerensis‘Lawsoniana'.
In this paper,two years old seedlings of Ilex aquifolium,two hybrids of Ilex aquifolium( Ilex × altaclerensis‘Belgica Aurea',Ilex × altaclerensis‘Lawsoniana') and two varieties of Ilex aquifolium( Ilex aquifolium.‘Madame Briot',Ilex aquifolium‘Little Gem') were placed in different low temperature conditions,the morphological characteristics,relative conductivity,the activity of superoxide( SOD),the level of malondialdehyde( MDA),the contents of soluble sugar and chlorophyll were studied. Comprehensive evaluation of cold resistance on five kinds of plants was conducted by principal component analysis and subordinate function analysis.The results revealed that cold resistance of five kinds of plants from strong to weak was Ilex × altaclerensis ‘Belgica Aurea'> Ilex aquifolium‘Little Gem'> Ilex aquifolium > Ilex aquifolium. ‘Madame Briot'> Ilex × altaclerensis‘Lawsoniana'.
引文
[1]ARANDA I,ROBSON T M,RODRíGUEZ-CALCERRADA J,et al.Limited capacity to cope with excessive light in the open and with seasonal drought in the shade in Mediterranean Ilex aquifolium populations[J]. Trees-Structure and Function,2008,22(3):375-384.
[2]曾雯,金晓玲,邢文,等. 9个常绿杂交冬青的抗寒能力比较[J].植物生理学报,2016,52(1):55-61.
[3]王学奎,黄见良.植物生理生化实验原理和技术(第3版)[M].北京:高等教育出版社,2015:34-202.
[4]朱根海,刘祖祺,朱培仁.应用Logistic方程确定植物组织低温半致死温度研究[J].南京农业大学学报,1986(3):11-16.
[5]杨克彬,孟凡志,郭先锋.七个切花红掌品种对低温胁迫的生理响应及耐低温能力评价[J].植物生理学报,2017,53(9):1609-1618.
[6]孙碧徽,李荣全,段小春.多年生黑麦草叶片形态与低温胁迫的生理响应[J].草业科学,2016,33(3):450-458.
[7]LYONS J M. Chilling injury in plants[J]. Annual Review of Plant Physiology,1973,24(1):445-466.
[8]PENG Y L,WANG Y S,FEI J,et al. Ecophysiological differences between three mangrove seedlings(Kandelia obovata,Aegiceras corniculatum,and Avicennia marina)exposed to chilling stress[J]. Ecotoxicology,2015,24(7/8):1722-1732.
[9]王运涛,于林清,李景柱,等.低温胁迫对5份苜蓿品种根系形态特征的影响[J].草地学报,2016,24(1):101-106.
[10]刘锦,王挺,黎念林,等.电导法配合Logistic方程测定樱花抗寒性研究[J].江苏林业科技,2016,43(5):25-27,31.
[11]张富玮,张东亚,李建贵.低温胁迫对蓝靛果忍冬抗寒性的影响[J].经济林业研究,2017,35(4):222-225,247.
[12]孙锐,孙蕾,赵彤,等.低温胁迫对我国北方地区引种的无花果抗寒性的影响[J].经济林研究,2017,35(3):55-62.
[13]曲彦婷,熊燕,韩辉,等.不同福禄考品种对低温胁迫的生理响应及抗寒性综合评价[J].植物生理学报,2016,52(4):487-496.
[14]李春燕,徐霞,刘立伟,等.低温条件下拔节期小麦叶片内源激素含量和抗氧化酶活性的变化[J].应用生态学报,2015,26(7):2015-2022.
[15]叶艳然,王文莉,郑成淑,等.四种野生苔草属植物的耐寒性评价[J].应用生态学报,2017,28(1):89-95.
[16]WANG N A,WANG K L,LIU Q H,et al. Study on cold resistance of three evergreen broad-leaved plants[J]. Acta Ecologica Sinica,2017,32(4):47-51.
[17]AMAGAI Y,KANEKO M,KUDO G. Habitat-specific responses of shoot growth and distribution of alpine dwarf-pine(Pinus pumila)to climate variation[J]. Ecological Research,2015,30(6):27-31.
[18]赵明明,周余华,彭方仁,等.低温胁迫下冬青叶片细胞内Ca2+水平及可溶性糖含量的变化[J].南京林业大学学报(自然科学版),2013,37(5):1-5.
[19]李桂荣,朱自果,马俊伟,等.低温胁迫对几种无核葡萄品种抗寒生理指标的影响[J].西北林学院学报,2015,30(5):75-78,291.
[20]董万鹏,罗充,龙秀琴,等.低温胁迫对西番莲抗寒生理指标的影响[J].植物生理学报,2015,51(5):771-777.
[21]邵怡若,许建新,薛立,等.低温胁迫时间对4种幼苗生理生化及光合特性的影响[J].生态学报,2013,33(14):4237-4247.
[22]李文明,魏一粟,钱艳萍,等. 5种酢浆草植物对低温胁迫的生理响应及抗寒性评价[J].东北林业大学学报,2017,45(7):28-33.
[23]杨再强,韩冬,王学林,等.寒潮过程中4个茶树品种光合特性和保护酶活性变化及品种间差异[J].生态学报,2016,36(3):629-641.
[24]黄浩,周祥斌,欧阳昆唏,等.黄粱木种源抗寒性综合评价[J].华南农业大学学报,2016,37(1):82-88.
[25]陆畅,李斌,刘儒,等.不同种源鹅掌楸抗寒性综合评价[J].浙江农林大学学报,2016,33(3):455-461.
[26]李瑞雪,金晓玲,胡希军,等. 6种含笑属植物抗寒性分析与综合评价[J].应用生态学报,2017,28(5):1464-1472.
[27]刘贝贝,陈利娜,牛娟,等. 6个石榴品种抗寒性评价及方法筛选[J].果树学报,2018,35(1):66-73.
[28]胡良.基于最优分割的南京季节划分法[J].科学技术创新,2017(27):11-13.
[2]曾雯,金晓玲,邢文,等. 9个常绿杂交冬青的抗寒能力比较[J].植物生理学报,2016,52(1):55-61.
[3]王学奎,黄见良.植物生理生化实验原理和技术(第3版)[M].北京:高等教育出版社,2015:34-202.
[4]朱根海,刘祖祺,朱培仁.应用Logistic方程确定植物组织低温半致死温度研究[J].南京农业大学学报,1986(3):11-16.
[5]杨克彬,孟凡志,郭先锋.七个切花红掌品种对低温胁迫的生理响应及耐低温能力评价[J].植物生理学报,2017,53(9):1609-1618.
[6]孙碧徽,李荣全,段小春.多年生黑麦草叶片形态与低温胁迫的生理响应[J].草业科学,2016,33(3):450-458.
[7]LYONS J M. Chilling injury in plants[J]. Annual Review of Plant Physiology,1973,24(1):445-466.
[8]PENG Y L,WANG Y S,FEI J,et al. Ecophysiological differences between three mangrove seedlings(Kandelia obovata,Aegiceras corniculatum,and Avicennia marina)exposed to chilling stress[J]. Ecotoxicology,2015,24(7/8):1722-1732.
[9]王运涛,于林清,李景柱,等.低温胁迫对5份苜蓿品种根系形态特征的影响[J].草地学报,2016,24(1):101-106.
[10]刘锦,王挺,黎念林,等.电导法配合Logistic方程测定樱花抗寒性研究[J].江苏林业科技,2016,43(5):25-27,31.
[11]张富玮,张东亚,李建贵.低温胁迫对蓝靛果忍冬抗寒性的影响[J].经济林业研究,2017,35(4):222-225,247.
[12]孙锐,孙蕾,赵彤,等.低温胁迫对我国北方地区引种的无花果抗寒性的影响[J].经济林研究,2017,35(3):55-62.
[13]曲彦婷,熊燕,韩辉,等.不同福禄考品种对低温胁迫的生理响应及抗寒性综合评价[J].植物生理学报,2016,52(4):487-496.
[14]李春燕,徐霞,刘立伟,等.低温条件下拔节期小麦叶片内源激素含量和抗氧化酶活性的变化[J].应用生态学报,2015,26(7):2015-2022.
[15]叶艳然,王文莉,郑成淑,等.四种野生苔草属植物的耐寒性评价[J].应用生态学报,2017,28(1):89-95.
[16]WANG N A,WANG K L,LIU Q H,et al. Study on cold resistance of three evergreen broad-leaved plants[J]. Acta Ecologica Sinica,2017,32(4):47-51.
[17]AMAGAI Y,KANEKO M,KUDO G. Habitat-specific responses of shoot growth and distribution of alpine dwarf-pine(Pinus pumila)to climate variation[J]. Ecological Research,2015,30(6):27-31.
[18]赵明明,周余华,彭方仁,等.低温胁迫下冬青叶片细胞内Ca2+水平及可溶性糖含量的变化[J].南京林业大学学报(自然科学版),2013,37(5):1-5.
[19]李桂荣,朱自果,马俊伟,等.低温胁迫对几种无核葡萄品种抗寒生理指标的影响[J].西北林学院学报,2015,30(5):75-78,291.
[20]董万鹏,罗充,龙秀琴,等.低温胁迫对西番莲抗寒生理指标的影响[J].植物生理学报,2015,51(5):771-777.
[21]邵怡若,许建新,薛立,等.低温胁迫时间对4种幼苗生理生化及光合特性的影响[J].生态学报,2013,33(14):4237-4247.
[22]李文明,魏一粟,钱艳萍,等. 5种酢浆草植物对低温胁迫的生理响应及抗寒性评价[J].东北林业大学学报,2017,45(7):28-33.
[23]杨再强,韩冬,王学林,等.寒潮过程中4个茶树品种光合特性和保护酶活性变化及品种间差异[J].生态学报,2016,36(3):629-641.
[24]黄浩,周祥斌,欧阳昆唏,等.黄粱木种源抗寒性综合评价[J].华南农业大学学报,2016,37(1):82-88.
[25]陆畅,李斌,刘儒,等.不同种源鹅掌楸抗寒性综合评价[J].浙江农林大学学报,2016,33(3):455-461.
[26]李瑞雪,金晓玲,胡希军,等. 6种含笑属植物抗寒性分析与综合评价[J].应用生态学报,2017,28(5):1464-1472.
[27]刘贝贝,陈利娜,牛娟,等. 6个石榴品种抗寒性评价及方法筛选[J].果树学报,2018,35(1):66-73.
[28]胡良.基于最优分割的南京季节划分法[J].科学技术创新,2017(27):11-13.