龟鹿益神颗粒对慢性疲劳综合征大鼠行为及相关雄性激素的影响
|
摘要
目的:观察龟鹿益神颗粒对慢性疲劳综合征(Chronic fatigue syndrome,CFS)大鼠行为学及血清睾酮(Testosterone,T)、双氢睾酮(Dihydrotestosterone,DHT)、硫酸脱氢表雄酮(Dehydroepiandrosterone sulfate,DHEAS)的影响,探讨龟鹿益神颗粒抗疲劳的作用机制。方法:雄性SD大鼠60只,随机分成正常组、模型组、苁蓉益肾颗粒组、龟鹿益神颗粒低、中、高剂量组,每组10只,采用慢性束缚、力竭游泳复合方法构建CFS大鼠模型,造模成功后,正常组、模型组每天给予10 m L/(kg·d)生理盐水灌服,其他各组给予相应药物灌服,龟鹿益神颗粒低、中、高剂量组,分别按照1.625 g/(kg·d),3.250 g/(kg·d),6.500 g/(kg·d)的剂量灌胃,苁蓉益肾颗粒组给予苁蓉益肾颗粒混悬液417 mg/(kg·d),每天1次,连续14天。造模前、后及灌胃后分别测量大鼠行为学指标,末次灌胃12 h后ELISA法测定各组大鼠血清T、DHT、DHEAS的含量。结果:造模前各组大鼠体质量、矿场跨格运动次数、力竭游泳时间无明显差异。造模后,与正常组比较,各组大鼠体质量、矿场跨格运动次数、力竭游泳时间均明显减少(P <0.05);给药后,与正常组比较,模型组大鼠体质量、矿场跨格运动次数、力竭游泳时间及血清T、DHT、DHEAS的含量明显降低(P <0.05,P <0.01);与模型组比较,龟鹿益神颗粒各剂量组和苁蓉益肾颗粒组大鼠体质重、旷场跨格次数、力竭游泳时间及血清T、DHT、DHEAS均有显著上升(P <0.05,P <0.01);与苁蓉益肾颗粒组相比,龟鹿益神颗粒中、高剂量组大鼠体质量、矿场跨格运动次数、力竭游泳时间及血清T、DHT、DHEAS的含量明显上升(P <0.05,P <0.01)。结论:龟鹿益神颗粒可显著改善CFS大鼠的疲劳状态,升高机体内T、DHT、DHEAS的含量,此可能是其抗疲劳机制之一。
Objective:To observe the effect of Guilu Yishen granules on behavior,testosterone(T),dihydrotestosterone(DHT) and dehydroepiandrosterone sulfate(DHEAS) in serum of rats with chronic fatigue syndrome(CFS), and discuss its functional mechanism of anti-fatigue. Methods:60 male SD rats were divided into the normal group,the model group,the Congrong Yishen granules group and the Guilu Yishen granules groups of low,middle and high dose randomly,10 rats in each group. The model of rats with CFS was established by composite method of chronic restraint and exhaustive swimming.After the establishment,the normal group and the model group received saline in 10 m L/(kg·d) via gavage every day,while the other groups received the corresponding medicine via gavage. The Guilu Yishen granules groups of low,middle and high dose received the suspension of Guilu Yishen granules in 1.625 g/(kg·d), 3.250 g/(kg·d) and 6.500 g/(kg·d) via gavage respectively,and the Congrong Yishen granules group received the suspension of Congrong Yishen granules in 417 mg/(kg·d)via gavage,1 time per day and for 14 days continuously. Measured the behavioral indexes of rats before and after the model establishment and after gavage,and detected the contents of T,DHT and DHEAS in serum of rats in each group after the last gavage for 12 hours via ELISA method. Results:Before the model establishment,comparing the body mass,frequency of crossing squares in open field and time of exhaustive swimming of rats in each group,there were no significant differences being found. After the model establishment, comparing with the normal group, the body mass, frequency of crossing squares in open field and time of exhaustive swimming of rats in the other groups were all significantly decreased(P < 0.05).After gavage, comparing with the normal group, the body mass, frequency of crossing squares in open field, time of exhaustive swimming and contents of T,DHT and DHEAS in serum of rats in the model group were all significantly decreased(P < 0.05,P < 0.01);comparing with the model group,the body mass,frequency of crossing squares in open field,time of exhaustive swimming and contents of T,DHT and DHEAS in serum of rats in the Guilu Yishen granules groups of each dose and the Congrong Yishen granules group were all significantly increased(P < 0.05,P < 0.01);comparing with the Congrong Yishen granules group, the body mass, frequency of crossing squares in open field, time of exhaustive swimming and contents of T, DHT and DHEAS in serum of rats in the Guilu Yishen granules groups of middle and high dose were all significantly increased(P < 0.05,P < 0.01). Conclusion:Guilu Yishen granules can significantly improve the fatigue sate of rats with CFS,and one of its functional mechanism may be promoting the contents of T,DHT and DHEAS in body.
Objective:To observe the effect of Guilu Yishen granules on behavior,testosterone(T),dihydrotestosterone(DHT) and dehydroepiandrosterone sulfate(DHEAS) in serum of rats with chronic fatigue syndrome(CFS), and discuss its functional mechanism of anti-fatigue. Methods:60 male SD rats were divided into the normal group,the model group,the Congrong Yishen granules group and the Guilu Yishen granules groups of low,middle and high dose randomly,10 rats in each group. The model of rats with CFS was established by composite method of chronic restraint and exhaustive swimming.After the establishment,the normal group and the model group received saline in 10 m L/(kg·d) via gavage every day,while the other groups received the corresponding medicine via gavage. The Guilu Yishen granules groups of low,middle and high dose received the suspension of Guilu Yishen granules in 1.625 g/(kg·d), 3.250 g/(kg·d) and 6.500 g/(kg·d) via gavage respectively,and the Congrong Yishen granules group received the suspension of Congrong Yishen granules in 417 mg/(kg·d)via gavage,1 time per day and for 14 days continuously. Measured the behavioral indexes of rats before and after the model establishment and after gavage,and detected the contents of T,DHT and DHEAS in serum of rats in each group after the last gavage for 12 hours via ELISA method. Results:Before the model establishment,comparing the body mass,frequency of crossing squares in open field and time of exhaustive swimming of rats in each group,there were no significant differences being found. After the model establishment, comparing with the normal group, the body mass, frequency of crossing squares in open field and time of exhaustive swimming of rats in the other groups were all significantly decreased(P < 0.05).After gavage, comparing with the normal group, the body mass, frequency of crossing squares in open field, time of exhaustive swimming and contents of T,DHT and DHEAS in serum of rats in the model group were all significantly decreased(P < 0.05,P < 0.01);comparing with the model group,the body mass,frequency of crossing squares in open field,time of exhaustive swimming and contents of T,DHT and DHEAS in serum of rats in the Guilu Yishen granules groups of each dose and the Congrong Yishen granules group were all significantly increased(P < 0.05,P < 0.01);comparing with the Congrong Yishen granules group, the body mass, frequency of crossing squares in open field, time of exhaustive swimming and contents of T, DHT and DHEAS in serum of rats in the Guilu Yishen granules groups of middle and high dose were all significantly increased(P < 0.05,P < 0.01). Conclusion:Guilu Yishen granules can significantly improve the fatigue sate of rats with CFS,and one of its functional mechanism may be promoting the contents of T,DHT and DHEAS in body.
引文
[1]金杰,郭智宽.从肾论治慢性疲劳综合征[J].光明中医,2010,25(11):1984-1985.
[2]郭智宽,金杰.金杰教授治疗慢性疲劳综合征经验[J].中医临床研究,2011,3(1):86-88.
[3]乔玉成,张红娟,卢向阳,等.补充五子壮阳汤对大运动量训练大鼠雄激素水平及运动能力的影响[J].中国运动医学杂志,2004,23(3):307-308.
[4]沈小珩,郑岚,朱伟嵘,等.苁蓉益肾颗粒治疗肾虚型慢性疲劳综合征的临床疗效观察[J].中国药房,2008,19(18):1416-1418.
[5]张惟郁,许建中,沈小珩.左归丸治疗肾虚型慢性疲劳综合征的临床观察[J].中成药, 2009, 31(12):1815-1817.
[6]沈自尹.从肾本质研究到证本质研究的思考与实践[J].上海中医药杂志,2000,34(4):4-7.
[7]孙广仁.中医基础理论[M].北京:中国中医药出版社,2007:122-124.
[8]董美仙.龟鹿二仙膏补肾抗衰的实验研究及“气助阴生阳长”的理论探讨[D].广州:广州中医药大学,2006.
[9]高学敏.中药学[M].北京:中国中医药出版社,2007:481.
[10]段琦梅,梁宗锁,聂小妮,等.黄芪和党参提取物的抗氧化活性研究[J].西北植物学报,2010,30(10):2123-2127.
[11]陈亚丹.党参多糖对铅中毒小鼠记忆障碍及免疫功能低下的影响[D].长春:吉林大学,2009.
[12]宋李亚,石君杰,梅诗雪,等.仙鹤草对抗大鼠运动性疲劳的实验研究[J].现代中西医结合杂志,2011,20(35):4481-4482.
[13]蒋兰,杨毅,江荣高.枸杞的药理作用及其加工现状[J].食品工业科技,2018,39(14):330-334.
[14]叶双双.龟鹿益神配方颗粒对慢性疲劳大鼠行为和骨骼肌线粒体的影响[D].郑州:河南中医学院,2014.
[15]王晨.龟鹿益神颗粒对慢性疲劳大鼠行为学和骨骼肌中PGC-1α的影响[D].郑州:河南中医学院,2014.
[16] Rutherford G,Manning P,Newton JL.Understanding Muscle Dysfunction in Chronic Fatigue Syndrome[J].Journal of Aging Research,2016,2016(4):2497348.
[17] Hollingsworth KG,Jones DE,Taylor R,et al.Impaired cardiovascular response to standing in Chronic Fatigue Syndrome[J].European Journal of Clinical Investigation,2010,40(7):608-615.
[18]郑陆,隋波,潘力平,等.过度训练大鼠骨骼肌总蛋白含量和雄激素受体结合容量的变化[J].中国运动医学杂志,2000,19(2):145-147.
[19] Baulieu EE,Lasnizki I,Robel P.Metabolism of testoesterone and action of metabo-lites on prostate glands grown in organ culture[J].Nature,1968,219(5159):1155-1156.
[20]宛霞,赵晓进.陈氏太极拳运动时血浆胰岛素、睾酮的变化[J].中国应用生理学杂志,1997,13(1):94-95.
[21]刘长征,雷波.针刺对慢性疲劳综合征大鼠血清丙二醛含量及超氧化物歧化酶、谷胱甘肽过氧化物酶活性的影响[J].针刺研究,2012,37(1):38-40,58.
[22]冯勤勤,赵玉杰,金杰,等.升阳益胃颗粒对慢性疲劳大鼠运动及血清SOD、MDA水平的影响[J].中医学报,2017,32(7):1223-1226.
[23]许淑静,高丽,陈国洪,等.36例婴儿痉挛患儿血清孕酮与硫酸脱氢表雄酮表达及药物影响的研究[J].中国小儿急救医学,2014,21(12):799-801.
[24]叶青,刘艳,欧阳凯,等.多囊卵巢综合征患者血清DHEA-S与SHBG检测的临床意义[J].热带医学杂志,2014,14(10):1309-1311.
[25]王雁,饶曼人.脱氢表雄酮硫酸酯对体外大鼠脑皮层神经细胞拟老化反应的影响[J].药学学报,1998,33(6):413-417.
[2]郭智宽,金杰.金杰教授治疗慢性疲劳综合征经验[J].中医临床研究,2011,3(1):86-88.
[3]乔玉成,张红娟,卢向阳,等.补充五子壮阳汤对大运动量训练大鼠雄激素水平及运动能力的影响[J].中国运动医学杂志,2004,23(3):307-308.
[4]沈小珩,郑岚,朱伟嵘,等.苁蓉益肾颗粒治疗肾虚型慢性疲劳综合征的临床疗效观察[J].中国药房,2008,19(18):1416-1418.
[5]张惟郁,许建中,沈小珩.左归丸治疗肾虚型慢性疲劳综合征的临床观察[J].中成药, 2009, 31(12):1815-1817.
[6]沈自尹.从肾本质研究到证本质研究的思考与实践[J].上海中医药杂志,2000,34(4):4-7.
[7]孙广仁.中医基础理论[M].北京:中国中医药出版社,2007:122-124.
[8]董美仙.龟鹿二仙膏补肾抗衰的实验研究及“气助阴生阳长”的理论探讨[D].广州:广州中医药大学,2006.
[9]高学敏.中药学[M].北京:中国中医药出版社,2007:481.
[10]段琦梅,梁宗锁,聂小妮,等.黄芪和党参提取物的抗氧化活性研究[J].西北植物学报,2010,30(10):2123-2127.
[11]陈亚丹.党参多糖对铅中毒小鼠记忆障碍及免疫功能低下的影响[D].长春:吉林大学,2009.
[12]宋李亚,石君杰,梅诗雪,等.仙鹤草对抗大鼠运动性疲劳的实验研究[J].现代中西医结合杂志,2011,20(35):4481-4482.
[13]蒋兰,杨毅,江荣高.枸杞的药理作用及其加工现状[J].食品工业科技,2018,39(14):330-334.
[14]叶双双.龟鹿益神配方颗粒对慢性疲劳大鼠行为和骨骼肌线粒体的影响[D].郑州:河南中医学院,2014.
[15]王晨.龟鹿益神颗粒对慢性疲劳大鼠行为学和骨骼肌中PGC-1α的影响[D].郑州:河南中医学院,2014.
[16] Rutherford G,Manning P,Newton JL.Understanding Muscle Dysfunction in Chronic Fatigue Syndrome[J].Journal of Aging Research,2016,2016(4):2497348.
[17] Hollingsworth KG,Jones DE,Taylor R,et al.Impaired cardiovascular response to standing in Chronic Fatigue Syndrome[J].European Journal of Clinical Investigation,2010,40(7):608-615.
[18]郑陆,隋波,潘力平,等.过度训练大鼠骨骼肌总蛋白含量和雄激素受体结合容量的变化[J].中国运动医学杂志,2000,19(2):145-147.
[19] Baulieu EE,Lasnizki I,Robel P.Metabolism of testoesterone and action of metabo-lites on prostate glands grown in organ culture[J].Nature,1968,219(5159):1155-1156.
[20]宛霞,赵晓进.陈氏太极拳运动时血浆胰岛素、睾酮的变化[J].中国应用生理学杂志,1997,13(1):94-95.
[21]刘长征,雷波.针刺对慢性疲劳综合征大鼠血清丙二醛含量及超氧化物歧化酶、谷胱甘肽过氧化物酶活性的影响[J].针刺研究,2012,37(1):38-40,58.
[22]冯勤勤,赵玉杰,金杰,等.升阳益胃颗粒对慢性疲劳大鼠运动及血清SOD、MDA水平的影响[J].中医学报,2017,32(7):1223-1226.
[23]许淑静,高丽,陈国洪,等.36例婴儿痉挛患儿血清孕酮与硫酸脱氢表雄酮表达及药物影响的研究[J].中国小儿急救医学,2014,21(12):799-801.
[24]叶青,刘艳,欧阳凯,等.多囊卵巢综合征患者血清DHEA-S与SHBG检测的临床意义[J].热带医学杂志,2014,14(10):1309-1311.
[25]王雁,饶曼人.脱氢表雄酮硫酸酯对体外大鼠脑皮层神经细胞拟老化反应的影响[J].药学学报,1998,33(6):413-417.