金露梅提取物对α-淀粉酶、α-葡萄糖苷酶和醛糖还原酶的抑制作用
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摘要
通过α-淀粉酶抑制剂、α-葡萄糖苷酶抑制剂和醛糖还原酶抑制剂体外筛选模型,研究金露梅水提物及95%甲醇提取石油醚萃取部位、乙酸乙酯萃取部位和水层部位对α-淀粉酶、α-葡萄糖苷酶和醛糖还原酶的抑制活性作用;通过糖耐量实验测定金露梅提取物对小鼠餐后血糖的影响。结果显示,金露梅水提物及95%甲醇提取石油醚萃取部位、乙酸乙酯萃取部位对α-淀粉酶的半数抑制浓度(IC_(50))分别为0.432、1.193、0._(50)7 mg/mL;对α-葡萄糖苷酶的IC_(50)分别为0.164、0.768、0.466 mg/mL;对醛糖还原酶的IC_(50)分别为0.742、2.158、1.098 mg/mL。结果表明,金露梅水提物和金露梅乙酸乙酯萃取部位对α-淀粉酶、α-葡萄糖苷酶和醛糖还原酶的抑制活性较高。同时,金露梅水提物与乙酸乙酯萃取部位均能显著降低小鼠的餐后血糖水平。
This study measured the in vitro inhibitory effects of α-amylase( AM),α-glycosidase( AG) and aldose reductase( AR) extraction from Potentilla fruticosa in three solvents: water extract( WE) and 95% methanol extraction of petroleum ether part( MEP),95% methanol extraction of ethyl acetate part( MEE) and 95% methanol extraction of water part( MEW) through α-amylase inhibitors( AMI),α-glycosidase inhibitors( AGI) and aldose reductase inhibitors( ARI) activity screening models. In vivo effects of different solvents from Potentilla fruticosa on impaired glucose tolerance of mice were also measured. Among them,WE,MEP and MEE exhibited against AMI activity with IC_(50) values of 0. 432,1. 193 and 0. _(50)7 mg/mL,respectively. Three solvents against AGI activity with IC_(50) values of 0. 164,0. 768 and 0. 466 mg/mL,respectively. Three solvents against ARI activity with IC_(50) values of 0. 742, 2. 158 and 1. 098 mg/mL, respectively. The study suggests that Potentilla fruticosa in water extract and 95% methanol extraction of ethyl acetate part demonstrated a stronger inhibitory effect on AM,AG and AR. Meanwhile,Potentilla fruticosa in water extract and 95% methanol extraction of ethyl acetate part can be significantly decreased the postprandial blood glucose in mice.
This study measured the in vitro inhibitory effects of α-amylase( AM),α-glycosidase( AG) and aldose reductase( AR) extraction from Potentilla fruticosa in three solvents: water extract( WE) and 95% methanol extraction of petroleum ether part( MEP),95% methanol extraction of ethyl acetate part( MEE) and 95% methanol extraction of water part( MEW) through α-amylase inhibitors( AMI),α-glycosidase inhibitors( AGI) and aldose reductase inhibitors( ARI) activity screening models. In vivo effects of different solvents from Potentilla fruticosa on impaired glucose tolerance of mice were also measured. Among them,WE,MEP and MEE exhibited against AMI activity with IC_(50) values of 0. 432,1. 193 and 0. _(50)7 mg/mL,respectively. Three solvents against AGI activity with IC_(50) values of 0. 164,0. 768 and 0. 466 mg/mL,respectively. Three solvents against ARI activity with IC_(50) values of 0. 742, 2. 158 and 1. 098 mg/mL, respectively. The study suggests that Potentilla fruticosa in water extract and 95% methanol extraction of ethyl acetate part demonstrated a stronger inhibitory effect on AM,AG and AR. Meanwhile,Potentilla fruticosa in water extract and 95% methanol extraction of ethyl acetate part can be significantly decreased the postprandial blood glucose in mice.
引文
[1]Fu LG,Hong T.Higher Plants of China(中国植物志)[M].Qingdao:Qingdao Pulishing House,2003,6:659.
[2]Qian L,Xiang H,You QD,et al.Outlines of anti-obesity drugs[J].Chin New Drugs J(中国新药杂志),2006,16(6):437-442.
[3]Meng XY,Guo SM,Yang LX.Effect of traditional Chinese medicine polysaccharides in resisting type 2 diabetes insulin[J].Chin J Exp Tradit Med Form(中药实验方剂学杂志),2017,23(8):220-225.
[4]Wu YH,Chen N,Zhang Q,et al.The hypoglycemic action ofα-amylase inhibition on fat rats[J].Chin J Bioprocess Eng(生物加工过程),2006,4(3):73-76.
[5]Wang W,Wang JH,Zhang XK,et al.Mechanism study of alpha glycosidase inhibitors and glycosidase[J].J Zaozhuang Univ(枣庄学院学报),2016,33(5):127-134.
[6]Jia J,Yu JY.Effects of Epalrestat on diabetic peripheral neuropathy:a meta-analysis[J].Chin J Diabetes(中国糖尿病杂志),2013,21(9):782-788.
[7]Sun YX,Zeng Y,Zhang QR,et al.Inhibition of Potentilla parvifolia on alpha-glucosidase and aldose reductase[J].J Beijing Univ Tradit Chin Med(北京中医药大学学报),2016,39(7):586-589.
[8]Wu J,Zhou BH.Optimization of quercetin extracting from Opuntia dillenii(Ker Gawl.)haw by orthogonal experiment[J].J Hubei Coll TCM(湖北中医学院学报),2006,8(3):25-26.
[9]Jia CY,Li WH,Chen X,et al.Extraction and determination of neohesperidin[J].Technol Develop Chem Ind(化工技术与开发),2007,36(12):45-46.
[10]Zhao YL.Improvement of determination method onα-amylase activity in wheat grain[J].Heilongjiang Agric Sic(黑龙江农业科学),2010(4):40-42.
[11]Lin MD,Wang GZ,Ye XY,et al.Screening of marine microorganisms producedα-amylase and its enzymatic properties[J].J Chin Inst Food Sci Technol(中国食品学报),2017,17(2):77-83.
[12]Zhang Q,Du Y,Chen GG,et al.Lipid-regulating and anti-atherosclerosis effects ofα-amylase inhibitor extracted from wheat[J].J China Pharm Univ(中国药科大学学报),2005,36(6):572-576.
[13]Li ZW,Chen HM,Chen C,et al.Efficacy ofα-glucosidase inhibitor in combination with SPP-4 inhibitor on type 2 diabetic mellitus[J].Chin J Diabetes(中国糖尿病杂志),2015,23(12):1070-1072.
[14]Lin K,Yin GS,Lin SD.Aldose reductase inhibitors and chronic diabetic complication[J].Chin J Diabetes(中国糖尿病杂志),2011,19(10):792-795.
[2]Qian L,Xiang H,You QD,et al.Outlines of anti-obesity drugs[J].Chin New Drugs J(中国新药杂志),2006,16(6):437-442.
[3]Meng XY,Guo SM,Yang LX.Effect of traditional Chinese medicine polysaccharides in resisting type 2 diabetes insulin[J].Chin J Exp Tradit Med Form(中药实验方剂学杂志),2017,23(8):220-225.
[4]Wu YH,Chen N,Zhang Q,et al.The hypoglycemic action ofα-amylase inhibition on fat rats[J].Chin J Bioprocess Eng(生物加工过程),2006,4(3):73-76.
[5]Wang W,Wang JH,Zhang XK,et al.Mechanism study of alpha glycosidase inhibitors and glycosidase[J].J Zaozhuang Univ(枣庄学院学报),2016,33(5):127-134.
[6]Jia J,Yu JY.Effects of Epalrestat on diabetic peripheral neuropathy:a meta-analysis[J].Chin J Diabetes(中国糖尿病杂志),2013,21(9):782-788.
[7]Sun YX,Zeng Y,Zhang QR,et al.Inhibition of Potentilla parvifolia on alpha-glucosidase and aldose reductase[J].J Beijing Univ Tradit Chin Med(北京中医药大学学报),2016,39(7):586-589.
[8]Wu J,Zhou BH.Optimization of quercetin extracting from Opuntia dillenii(Ker Gawl.)haw by orthogonal experiment[J].J Hubei Coll TCM(湖北中医学院学报),2006,8(3):25-26.
[9]Jia CY,Li WH,Chen X,et al.Extraction and determination of neohesperidin[J].Technol Develop Chem Ind(化工技术与开发),2007,36(12):45-46.
[10]Zhao YL.Improvement of determination method onα-amylase activity in wheat grain[J].Heilongjiang Agric Sic(黑龙江农业科学),2010(4):40-42.
[11]Lin MD,Wang GZ,Ye XY,et al.Screening of marine microorganisms producedα-amylase and its enzymatic properties[J].J Chin Inst Food Sci Technol(中国食品学报),2017,17(2):77-83.
[12]Zhang Q,Du Y,Chen GG,et al.Lipid-regulating and anti-atherosclerosis effects ofα-amylase inhibitor extracted from wheat[J].J China Pharm Univ(中国药科大学学报),2005,36(6):572-576.
[13]Li ZW,Chen HM,Chen C,et al.Efficacy ofα-glucosidase inhibitor in combination with SPP-4 inhibitor on type 2 diabetic mellitus[J].Chin J Diabetes(中国糖尿病杂志),2015,23(12):1070-1072.
[14]Lin K,Yin GS,Lin SD.Aldose reductase inhibitors and chronic diabetic complication[J].Chin J Diabetes(中国糖尿病杂志),2011,19(10):792-795.