TY病毒病与番茄叶片金属元素含量的关系研究
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摘要
为明确番茄黄化曲叶病毒病(TY病毒病)与番茄叶片中各金属元素含量的关系,利用原子吸收分光光度计对不同品种及不同定植时间的番茄叶片中Mg、Ca、Fe、Mn、Cu、Zn共6种金属元素的含量进行了测定。研究结果表明:不同抗性水平的番茄品种的各金属元素含量存在显著性差异,番茄的病情指数与Mg、Mn和Zn 3种元素的含量呈线性负相关关系,且随着定植时间的增加,感病品种叶片中Mn和Zn的含量呈显著性降低。因此,在番茄生长过程中,为了抑制番茄黄化曲叶病毒病的发生,可对植株施加适量的Mn肥和Zn肥。
To clarify the relationship between tomato yellow leaf curl virus(TYLCV) and metal element contents in tomato leaves, atomic absorption spectrophotometer was used to determine the contents of Mg, Ca,Fe, Mn, Cu and Zn in leaves of different tomato varieties and different planting time. The results suggested that there were significant differences in the contents of various metal elements of tomato varieties with different resistance levels, and a negative linear correlation between the disease index and the contents of Mg, Mn and Zn. The contents of Mn and Zn in the leaves of affected varieties reduced significantly with the prolongation of planting time. Accordingly, to inhibit the development of TYLCV during tomatoes growth, appropriate amounts of Mn and Zn fertilizers could be applied to the plants.
To clarify the relationship between tomato yellow leaf curl virus(TYLCV) and metal element contents in tomato leaves, atomic absorption spectrophotometer was used to determine the contents of Mg, Ca,Fe, Mn, Cu and Zn in leaves of different tomato varieties and different planting time. The results suggested that there were significant differences in the contents of various metal elements of tomato varieties with different resistance levels, and a negative linear correlation between the disease index and the contents of Mg, Mn and Zn. The contents of Mn and Zn in the leaves of affected varieties reduced significantly with the prolongation of planting time. Accordingly, to inhibit the development of TYLCV during tomatoes growth, appropriate amounts of Mn and Zn fertilizers could be applied to the plants.
引文
[1]蔡健和,王苏燕,王小凤,等.番茄曲叶病及其血清学和PCR测定[J].微生物学报,1995,35(5):394-396.
[2]李延刚,李长松.番茄黄化曲叶病毒病研究进展[J].中国农学通报,2011,27(2):90-94.
[3]张锋,刘晨,杨苗苗,等.关中地区温室越冬茬番茄黄化曲叶病毒病分子鉴定及流行规律[J].植物保护学报,2017,44(2):246-252.
[4]Zrachya A,Glick E,Levy Y,et al.Suppressor of RNA silencing encoded by tomato yellow leaf curl virus-Israel[J].Virology,2007,358(1):159-165.
[5]Navot N,Pichersky E,Zeidan M,et al.Tomato yellow leaf curl virus:A whitefly-transmitted geminivirus with a single genomic component[J].Virology,1991,185(1):151-161.
[6]Zhang J,Dong J,Xu Y,et al.V2 protein encoded by tomato yellow leaf curl China virus is an RNA silencing suppressor[J].Virus Res,2012,163(1):51-58.
[7]Lefeuvre P,Hoareau M,Delatte H,et al.A multiplex PCR method discriminating between the TYLCV and TYLCV-Mld clades of tomato yellow leaf curl virus[J].Journal of Virological Methods,2003,112:35-40.
[8]Zhang Y P,Zhu W M,Cui H M.Molecular identification and the complete nucleotide sequence of TYLCV isolate from Shanghai of China[J].Virus Genes,2008,36:547-551.
[9]张翔,俞风娟,王继涛,等.日光温室春夏茬番茄抗黄化曲叶病毒病品种筛选试验[J].宁夏农林科技,2017,58(03):23-25.
[10]李英梅,陈志杰,张锋,等.不同番茄品种对TY病毒的抗性评价[J].西北农业学报,2015,24(8):139-144.
[11]毛亮,李海峰,潘卫萍,等.秋季温室不同番茄品种抗黄化曲叶病毒病抗性评价[J].北方园艺,2016,(03):109-112.
[12]苗相伟.番茄黄化曲叶病毒病的研究进展[J].中国果菜,2017,37(3):31-35.
[13]尹贤贵.间作对番茄黄化卷叶病毒病发生的影响[J].西南农业学报,2002,15(2):54-58.
[14]何顺.番茄黄化曲叶病毒病的化学防治研究[D].北京:中国农业大学,2014.
[15]Ji Y F,Schuster D J,Scott J W.Ty-3,a begomovirus resistance locus near the tomato yellow leaf curl virus resistance locus Ty-1on chromosome 6 of tomato[J].Molecular Breeding,2007,20:271-284.
[16]Ji Y F,Scott J W,Maxwell D P.Ty-4,a tomato yellow leaf curl virus resistance gene on chromosome 3 of tomato[J].Tomato Genet Coop Rep,2008,58:29-31.
[17]Garcia B E,Graham E,Jensen K S.Codominant SCAR marker for detection of the Begomovirus-resistance Ty-2 locus derived from Solanum habrochaitesin[J].Tomato Germplasm,2008(12):28.
[18]张前荣,朱海生,刘建汀,等.番茄种质抗黄化曲叶病毒病Ty-1基因的分子标记分析与田间抗病评价[J].福建农业学报,2016,31(01):36-39.
[19]Lapidot M,Friedmann M.Breeding for resistance to whiteflytransmitted geminiviruses[J].Annals of Applied Biology,2001,140:109-127.
[20]郭衍银,徐坤,王秀峰,等.矿质营养与植物病害机理研究进展[J].甘肃农业大学学报,2003,38(4):385-393.
[21]田永强,黄丽萍,张正.矿质元素缺失或不平衡与植物病害发生关系研究进展[J].中国农学通报,2016,32(21):174-176.
[22]杨文渊,谢红江,陶炼,等.苹果树腐烂病发生与土壤养分关系的研究[J].安徽农业科学,2015,43(33):51-53.
[23]胡增丽,聂磊云,王媛.微量元素含量与苹果树病害的关系分析[J].农业工程技术,2017,37(02):26.
[24]于忠范,杜兴政,崔丽静,等.苹果小叶病的发生原因与防治措施[J].烟台果树,2011,(01):45-46.
[25]Wei J,He YZ,Guo Q,et al.Vector development and vitellogenin determine the transovarial transmission of begomoviruses[J].PNAS,2017,114(26):6746-6751.
[26]宋亚茜.Cd与Zn长期共同胁迫对麦长管蚜种群生态系统影响的研究[D].杨凌:西北农林科技大学,2016.
[27]尹素真.Cu、Zn对白星花金龟幼虫的毒性效应[D].泰安:山东农业大学,2016.
[28]夏嫱,舒迎花,胡新军,等.斜纹夜蛾幼虫不同组织对Zn2+积累作用的比较研究[J].中山大学学报:自然科学版,2008,(03):89-93.
[2]李延刚,李长松.番茄黄化曲叶病毒病研究进展[J].中国农学通报,2011,27(2):90-94.
[3]张锋,刘晨,杨苗苗,等.关中地区温室越冬茬番茄黄化曲叶病毒病分子鉴定及流行规律[J].植物保护学报,2017,44(2):246-252.
[4]Zrachya A,Glick E,Levy Y,et al.Suppressor of RNA silencing encoded by tomato yellow leaf curl virus-Israel[J].Virology,2007,358(1):159-165.
[5]Navot N,Pichersky E,Zeidan M,et al.Tomato yellow leaf curl virus:A whitefly-transmitted geminivirus with a single genomic component[J].Virology,1991,185(1):151-161.
[6]Zhang J,Dong J,Xu Y,et al.V2 protein encoded by tomato yellow leaf curl China virus is an RNA silencing suppressor[J].Virus Res,2012,163(1):51-58.
[7]Lefeuvre P,Hoareau M,Delatte H,et al.A multiplex PCR method discriminating between the TYLCV and TYLCV-Mld clades of tomato yellow leaf curl virus[J].Journal of Virological Methods,2003,112:35-40.
[8]Zhang Y P,Zhu W M,Cui H M.Molecular identification and the complete nucleotide sequence of TYLCV isolate from Shanghai of China[J].Virus Genes,2008,36:547-551.
[9]张翔,俞风娟,王继涛,等.日光温室春夏茬番茄抗黄化曲叶病毒病品种筛选试验[J].宁夏农林科技,2017,58(03):23-25.
[10]李英梅,陈志杰,张锋,等.不同番茄品种对TY病毒的抗性评价[J].西北农业学报,2015,24(8):139-144.
[11]毛亮,李海峰,潘卫萍,等.秋季温室不同番茄品种抗黄化曲叶病毒病抗性评价[J].北方园艺,2016,(03):109-112.
[12]苗相伟.番茄黄化曲叶病毒病的研究进展[J].中国果菜,2017,37(3):31-35.
[13]尹贤贵.间作对番茄黄化卷叶病毒病发生的影响[J].西南农业学报,2002,15(2):54-58.
[14]何顺.番茄黄化曲叶病毒病的化学防治研究[D].北京:中国农业大学,2014.
[15]Ji Y F,Schuster D J,Scott J W.Ty-3,a begomovirus resistance locus near the tomato yellow leaf curl virus resistance locus Ty-1on chromosome 6 of tomato[J].Molecular Breeding,2007,20:271-284.
[16]Ji Y F,Scott J W,Maxwell D P.Ty-4,a tomato yellow leaf curl virus resistance gene on chromosome 3 of tomato[J].Tomato Genet Coop Rep,2008,58:29-31.
[17]Garcia B E,Graham E,Jensen K S.Codominant SCAR marker for detection of the Begomovirus-resistance Ty-2 locus derived from Solanum habrochaitesin[J].Tomato Germplasm,2008(12):28.
[18]张前荣,朱海生,刘建汀,等.番茄种质抗黄化曲叶病毒病Ty-1基因的分子标记分析与田间抗病评价[J].福建农业学报,2016,31(01):36-39.
[19]Lapidot M,Friedmann M.Breeding for resistance to whiteflytransmitted geminiviruses[J].Annals of Applied Biology,2001,140:109-127.
[20]郭衍银,徐坤,王秀峰,等.矿质营养与植物病害机理研究进展[J].甘肃农业大学学报,2003,38(4):385-393.
[21]田永强,黄丽萍,张正.矿质元素缺失或不平衡与植物病害发生关系研究进展[J].中国农学通报,2016,32(21):174-176.
[22]杨文渊,谢红江,陶炼,等.苹果树腐烂病发生与土壤养分关系的研究[J].安徽农业科学,2015,43(33):51-53.
[23]胡增丽,聂磊云,王媛.微量元素含量与苹果树病害的关系分析[J].农业工程技术,2017,37(02):26.
[24]于忠范,杜兴政,崔丽静,等.苹果小叶病的发生原因与防治措施[J].烟台果树,2011,(01):45-46.
[25]Wei J,He YZ,Guo Q,et al.Vector development and vitellogenin determine the transovarial transmission of begomoviruses[J].PNAS,2017,114(26):6746-6751.
[26]宋亚茜.Cd与Zn长期共同胁迫对麦长管蚜种群生态系统影响的研究[D].杨凌:西北农林科技大学,2016.
[27]尹素真.Cu、Zn对白星花金龟幼虫的毒性效应[D].泰安:山东农业大学,2016.
[28]夏嫱,舒迎花,胡新军,等.斜纹夜蛾幼虫不同组织对Zn2+积累作用的比较研究[J].中山大学学报:自然科学版,2008,(03):89-93.