基于HPLC-UV-CL联用分析技术评价不同产地山麦冬清除过氧化氢活性
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摘要
采用过氧化氢(H2O2)生成体系,以高效液相色谱-紫外-可见分光光度法-化学发光(HPLC-UV-CL)联用技术在线分析不同产地的山麦冬清除H2O2的作用,明确不同产地山麦冬乙醚部位直接清除H2O2的抗氧化活性特点。利用2种检测方法对不同产地的山麦冬的指纹图谱进行相似度评价分析。结果发现短葶山麦冬和湖北麦冬的乙醚部位均具有较好的清除过氧化氢活性,不同种之间的总活性差异较大,而同种样品间具有相似的活性,短葶山麦冬总活性较湖北麦冬高。2种检测方法对不同产地的山麦冬的指纹相似度比较分析,化学指纹与活性指纹具有不同的特征,各指纹对于总峰面积和总活性的贡献也不尽相同,2种不同指纹图谱聚类结果存在显著差异。
The hydrogen peroxide generation system was used to analyze the scavenging activity of hydrogen peroxide by Liropes Radix from different origins by HPLC-UV-CL. The UV-CL fingerprints of Liropes Radix from different origins were evaluated,and the HPLC-UV and LC-CL fingerprints were systematically analyzed and evaluated. The results showed that the ether fractions of Liriope spicata var. prolifera and L. muscari had good scavenging activity of hydrogen peroxide,and the total activity of different origins varied greatly,while the similar samples had similar activities. The total antioxidant activity of L. muscari is higher than that of L. spicata var.prolifera. The similarity analysis of the two fingerprints was carried out by two different analytical methods. The chemical fingerprints and the active fingerprints have different characteristics. The contribution of each fingerprint to the total peak area and total activity is also different. There are significant differences between the two different fingerprint clustering results.
The hydrogen peroxide generation system was used to analyze the scavenging activity of hydrogen peroxide by Liropes Radix from different origins by HPLC-UV-CL. The UV-CL fingerprints of Liropes Radix from different origins were evaluated,and the HPLC-UV and LC-CL fingerprints were systematically analyzed and evaluated. The results showed that the ether fractions of Liriope spicata var. prolifera and L. muscari had good scavenging activity of hydrogen peroxide,and the total activity of different origins varied greatly,while the similar samples had similar activities. The total antioxidant activity of L. muscari is higher than that of L. spicata var.prolifera. The similarity analysis of the two fingerprints was carried out by two different analytical methods. The chemical fingerprints and the active fingerprints have different characteristics. The contribution of each fingerprint to the total peak area and total activity is also different. There are significant differences between the two different fingerprint clustering results.
引文
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