低温胁迫水稻幼芽生长及生理响应
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摘要
【目的】为了解不同水稻材料和杂交后代的抗寒生理特性,筛选较为抗寒的水稻品种。【方法】选用12个杂交组合及材料,将萌发幼芽在6、9和12℃低温胁迫处理5 d,25℃作为对照。低温处理后恢复生长7 d测其成活率和芽长,测定幼芽丙二醛(MDA)、过氧化氢(H_2O_2)、过氧化物(O~-_2)、抗坏血酸(AsA)、脯氨酸(Pro)和可溶性糖(SS)含量。【结果】在低温处理下,各生理指标皆存在不同程度的上升。在6、9、12和25℃处理,MDA和AsA平均含量依次降低;12℃时,Pro、O~-_2和SS平均含量低于对照;H_2O_2平均含量在9℃达到最高。Pro变化幅度最大,O~-_2最小,在同一温度下,杂交组合及材料间存在较大差异。低温处理下杂交组合及材料成活率降低且幼芽生长缓慢,12、9和6℃时,成活率分别降低7.72%、30.40%和50.44%;芽长分别降低40.81%、61.98%和56.71%。【结论】糯稻89-1的抗寒性最好,优良恢复系品种为父本杂交产生的后代也具有较好的抗寒性状,可进行进一步筛选。
【Objective】The present paper aimed to understand the cold resistance physiological characteristics of different rice materials and hybridized combinations, and filter has good cold resistant varieties of rice. 【Method】Sprouting shoots of rice from 12 hybridized combinations and materials were treated under low temperature of 6, 9 and 12 ℃, respectively. The treatment at 25 ℃ was used as a control. After 5 days of three different temperature treatments, two agronomic traits(i.e. survival rate and shoot length) and five physiological indexes(i.e. the content of malondialdehyde(MDA), hydrogen peroxide(H_2O_2), peroxidase(O~-_2), ascorbic acid(AsA), proline(Pro) and soluble sugar(SS)) were measured.【Result】Under low temperature treatment, all physiological indexes increased to different degrees. The average content of MDA and AsA decreased as the increases of temperature treatments. The average content of Pro>O~-_2 and SS was lower than control's at 12 ℃. The average content of H_2O_(2 )reached the maximum under the treatment of 9 ℃. Of these, the reduced amplitude of Pro was maximum. On the contrary, the reduced amplitude of O~-_2was minimum. There was big difference between the hybridized combinations and materials under the same temperature treatment. The survival rate and shoot length of hybridized combinations and materials under low temperature stress were low compared to those of control, the survival rate of 12, 9 and 6 ℃ were reduced by 7.72 %, 30.40 % and 50.44 % compared with 25 ℃,the shoot length of 12, 9 and 6 ℃ were reduced by 40.81 %, 61.98 % and 56.71 % compared with 25 ℃. 【Conclusion】Glutinous rice 89-1 has the best cold resistance, and the hybridized progeny that had a male parent with superior restorer line would have a better cold resistance character, which could be further filter.
【Objective】The present paper aimed to understand the cold resistance physiological characteristics of different rice materials and hybridized combinations, and filter has good cold resistant varieties of rice. 【Method】Sprouting shoots of rice from 12 hybridized combinations and materials were treated under low temperature of 6, 9 and 12 ℃, respectively. The treatment at 25 ℃ was used as a control. After 5 days of three different temperature treatments, two agronomic traits(i.e. survival rate and shoot length) and five physiological indexes(i.e. the content of malondialdehyde(MDA), hydrogen peroxide(H_2O_2), peroxidase(O~-_2), ascorbic acid(AsA), proline(Pro) and soluble sugar(SS)) were measured.【Result】Under low temperature treatment, all physiological indexes increased to different degrees. The average content of MDA and AsA decreased as the increases of temperature treatments. The average content of Pro>O~-_2 and SS was lower than control's at 12 ℃. The average content of H_2O_(2 )reached the maximum under the treatment of 9 ℃. Of these, the reduced amplitude of Pro was maximum. On the contrary, the reduced amplitude of O~-_2was minimum. There was big difference between the hybridized combinations and materials under the same temperature treatment. The survival rate and shoot length of hybridized combinations and materials under low temperature stress were low compared to those of control, the survival rate of 12, 9 and 6 ℃ were reduced by 7.72 %, 30.40 % and 50.44 % compared with 25 ℃,the shoot length of 12, 9 and 6 ℃ were reduced by 40.81 %, 61.98 % and 56.71 % compared with 25 ℃. 【Conclusion】Glutinous rice 89-1 has the best cold resistance, and the hybridized progeny that had a male parent with superior restorer line would have a better cold resistance character, which could be further filter.
引文
[1]杨川航,王开,杨航,等.水稻耐寒育种研究进展[J].中国农学通报,2009,25(6):113-116.
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[9]王颖,王晓云,江志红,等.中国低温雨雪冰冻灾害危险性评估与区划[J].气象,2013,39(5):585-591.
[10]王晓煜,杨晓光,吕 ,等.全球气候变暖对中国种植制度可能影响Ⅻ·气候变暖对黑龙江寒地水稻安全种植区域和冷害风险的影响[J].中国农业科学,2016,49(10):1859-1871.
[11]赵正武,李仕贵,雷树凡.糯稻89-1越冬性遗传研究[J].中国农业科学,2006,39(12):2399-2405.
[12]李合生,孙群,赵世杰,等.植物生理生化试验原理和技术[M].北京:北京高等教育出版,2000:182-261.
[13]张宪政.作物生理研究法[M].北京:北京农业出版社,1992,197-212.
[14]Gillespie K M,Ainsworth E A.Measurement of reduced,oxidized and total ascorbate content in plants[J].Nature Protocols,2007,2(4):871-874.
[15]Patterson B D,Macrae E A,Ferguson I B.Estimation of hydrogen peroxide in plant extracts using titanium(IV)[J].Analytical Biochemistry,1984,139(2):487-492.
[16]王爱国,罗广华.植物的超氧物自由基与羟胺反应的定量关系[J].植物生理学通讯,1990(6):55-57.
[17]Los D A,Murata N.Membrane fluidity and its roles in the perception of environmental signals[J].Biochim Biophys Acta,2004,1666(1):142-157.
[18]王国骄,王嘉宇,苗微,等.强耐冷性水稻新品系J07-23 抗氧化系统对长期冷水胁迫的响应[J].作物学报,2013,39(4):753-759.
[19]熊俊兰,谭瑞,孔海燕,等.植物ABA-H2O2介导的气孔关闭[J].植物生理学报,2012,48(8):739-746.
[20]Gill S S,Tuteja N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J].Plant Physiol Biochem,2010,48(12):909-930.
[21]Pamplona R.Advanced lipoxidation end-products[J].Chemico-Biological Interactions,2011,192(1):14-20.
[22]邓仁菊,范建新,王永清,等.火龙果幼苗对低温胁迫的生理响应及其抗寒性综合评价[J].植物生理学报,2014,50(10):1529-1534.
[23]Blokhina O,Vriolainem E,Fagerstedt K V.Antioxidants,oxidative damage and oxygen deprivation stress:a review[J].Annals of Botany,2003,91(2):179-194.
[24]Smirnoff F N.Antioxidants and Reactive Oxygen Species in Plants[M].UK:Blackwell Publishing Ltd.,2005:53-86.
[25]Gallie D R.The role of L-ascorbic acid recycling in responding to environmental stress and in promoting plant growth[J].Journal of Experimental Botany,2013,64(2):433-443.
[26]范美华,张义鑫,石戈,等.外源抗坏血酸对油菜种子在海水胁迫下萌发生长的影响[J].中国油料作物学报,2009,31(1):34-38.
[27]阿地力·衣克木,巴哈尔古丽·阿尤甫,哈尔肯·叶尔江,等.不同北美海棠品种对低温胁迫的生理响应[J].西南农业学报,2018(4):687-692.
[28]Koehler G,Wilson R C,Goodpaster J V,et al.Proteomic study of low-temperature responses in strawberry cultivars(Fragaria×ananassa) that differ in cold tolerance[J].Plant Physiol,2012,159(4):1787-1805.
[29]Morsy M R,Jouve L,Hausman J F,et al.Alteration of oxidative and carbohydrate metabolism under abiotic stress in two rice(Oryza sativa L.) genotypes contrasting in chilling tolerance[J].Journal of Plant Physiology,2007,164(2):157-167.
[30]Kim S I,Tai T H.Evaluation of seedling cold tolerance in rice cultivars:a comparison of visual ratings and quantitative indicators of physiological changes[J].Euphytica,2011,178(3):437-447.
[2]宋涛,高艳,张明坤,等.水稻低温冷害研究现状探讨[J].现代农业科技,2016(14):56-57.
[3]武琦,邹德堂,赵宏伟,等.不同生育时期低温胁迫下水稻耐冷指标变化的研究[J].作物杂志,2012 (6):95-101.
[4]李霞,戴传超,程睿,等.不同生育期水稻耐冷性的鉴定及耐冷性差异的生理机制[J].作物学报,2006,32(1):732–739.
[5]Zhang Q,Chen Q,Wang S,et al.Rice and cold stress:methods for its evaluation and summary of cold tolerance-related quantitative trait loci[J].Rice,2014,7(1):24-35.
[6]王国莉,郭振飞.低温对水稻不同耐冷品种幼苗光合速率和叶绿素荧光参数的影响[J].中国水稻科学,2005,19 (4) :381-383.
[7]李健陵,霍治国,吴丽姬,等.孕穗期低温对水稻产量的影响及其生理机制[J].中国水稻科学,2014,28(3):277-278.
[8]周曙东,朱红根.气候变化对中国南方水稻产量的经济影响及其适应策略[J].中国人口·资源与境,2010,122(10):152-157.
[9]王颖,王晓云,江志红,等.中国低温雨雪冰冻灾害危险性评估与区划[J].气象,2013,39(5):585-591.
[10]王晓煜,杨晓光,吕 ,等.全球气候变暖对中国种植制度可能影响Ⅻ·气候变暖对黑龙江寒地水稻安全种植区域和冷害风险的影响[J].中国农业科学,2016,49(10):1859-1871.
[11]赵正武,李仕贵,雷树凡.糯稻89-1越冬性遗传研究[J].中国农业科学,2006,39(12):2399-2405.
[12]李合生,孙群,赵世杰,等.植物生理生化试验原理和技术[M].北京:北京高等教育出版,2000:182-261.
[13]张宪政.作物生理研究法[M].北京:北京农业出版社,1992,197-212.
[14]Gillespie K M,Ainsworth E A.Measurement of reduced,oxidized and total ascorbate content in plants[J].Nature Protocols,2007,2(4):871-874.
[15]Patterson B D,Macrae E A,Ferguson I B.Estimation of hydrogen peroxide in plant extracts using titanium(IV)[J].Analytical Biochemistry,1984,139(2):487-492.
[16]王爱国,罗广华.植物的超氧物自由基与羟胺反应的定量关系[J].植物生理学通讯,1990(6):55-57.
[17]Los D A,Murata N.Membrane fluidity and its roles in the perception of environmental signals[J].Biochim Biophys Acta,2004,1666(1):142-157.
[18]王国骄,王嘉宇,苗微,等.强耐冷性水稻新品系J07-23 抗氧化系统对长期冷水胁迫的响应[J].作物学报,2013,39(4):753-759.
[19]熊俊兰,谭瑞,孔海燕,等.植物ABA-H2O2介导的气孔关闭[J].植物生理学报,2012,48(8):739-746.
[20]Gill S S,Tuteja N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J].Plant Physiol Biochem,2010,48(12):909-930.
[21]Pamplona R.Advanced lipoxidation end-products[J].Chemico-Biological Interactions,2011,192(1):14-20.
[22]邓仁菊,范建新,王永清,等.火龙果幼苗对低温胁迫的生理响应及其抗寒性综合评价[J].植物生理学报,2014,50(10):1529-1534.
[23]Blokhina O,Vriolainem E,Fagerstedt K V.Antioxidants,oxidative damage and oxygen deprivation stress:a review[J].Annals of Botany,2003,91(2):179-194.
[24]Smirnoff F N.Antioxidants and Reactive Oxygen Species in Plants[M].UK:Blackwell Publishing Ltd.,2005:53-86.
[25]Gallie D R.The role of L-ascorbic acid recycling in responding to environmental stress and in promoting plant growth[J].Journal of Experimental Botany,2013,64(2):433-443.
[26]范美华,张义鑫,石戈,等.外源抗坏血酸对油菜种子在海水胁迫下萌发生长的影响[J].中国油料作物学报,2009,31(1):34-38.
[27]阿地力·衣克木,巴哈尔古丽·阿尤甫,哈尔肯·叶尔江,等.不同北美海棠品种对低温胁迫的生理响应[J].西南农业学报,2018(4):687-692.
[28]Koehler G,Wilson R C,Goodpaster J V,et al.Proteomic study of low-temperature responses in strawberry cultivars(Fragaria×ananassa) that differ in cold tolerance[J].Plant Physiol,2012,159(4):1787-1805.
[29]Morsy M R,Jouve L,Hausman J F,et al.Alteration of oxidative and carbohydrate metabolism under abiotic stress in two rice(Oryza sativa L.) genotypes contrasting in chilling tolerance[J].Journal of Plant Physiology,2007,164(2):157-167.
[30]Kim S I,Tai T H.Evaluation of seedling cold tolerance in rice cultivars:a comparison of visual ratings and quantitative indicators of physiological changes[J].Euphytica,2011,178(3):437-447.