黄体期短期优饲对道寒杂交羊卵泡发育的影响
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摘要
为研究母羊黄体期短期优饲对其卵泡发育的影响。选择60只道寒杂交羊随机分为试验组和对照组,每组30只。试验羊均先饲喂基础日粮(DE 11.72 MJ/d,DP 79.71 g/d),并对试验羊进行同期发情处理(肌注PG 0.1 mg,3 d后阴道埋置CIDR 12 d,撤栓再次肌注PG 0.1 mg),同期发情处理埋栓2 d后试验组羊饲喂试验日粮(DE 18.75 MJ/d,DP 108.44 g/d),饲喂期10 d,并于饲喂开始第1,2,4,6,8,10天分别采集试验组和对照组羊颈静脉血,测定血浆中葡萄糖,胰岛素,瘦素等含量。在撤栓注射PG后,埋栓第13,14,15天每组随机选择6只羊进行屠宰,采集卵巢,按小卵泡(≤3.0 mm)、中等卵泡(3.0～5.0 mm)和大卵泡(≥5.0 mm)进行卵泡数量统计及卵泡液、颗粒细胞收集。采用实时定量PCR技术,分析不同大小卵泡颗粒细胞中OB-R和IGF-1基因mRNA表达水平。结果显示,与对照组相比,试验组小卵泡数量显著下降(P<0.01),中等卵泡和大卵泡数量显著升高(P<0.01),同时血浆和大卵泡中葡萄糖(P<0.01)、胰岛素(P<0.01)和瘦素(P<0.05)的平均浓度均显著升高,中等卵泡和大卵泡颗粒细胞中OB-R和IGF-1基因的表达显著上升(P<0.05)。结果表明,母羊黄体期短期优饲可提高血浆中葡萄糖、胰岛素、瘦素浓度和颗粒细胞中OB-R和IGF-1基因表达量,促进卵泡期的卵泡优势化数量。
The study was to evaluate the effects of short-term nutritional supplementation on follicular development during luteal phase in ewes.60 Dorset×Han crossbred ewes were divided randomly into two groups of control(n=30) and treatment(n=30).All ewes were fed on basal diet(DE 11.72 MJ/d;DP 79.71 g/d) originally.Then ewes was treated to oestrus synchronization(3 d after intramuscularly injection of PG 0.1 mg,CIDR for 12 d,then PG injection again for the same dose on the day of removal of CIDR).From day 2 of CIDR embedment,a high-energy supplement diet(DE18.75 MJ/d;DP 108.44 g/d) was fed to the treatment group for 10 d.Plasma metabolites(glucose,insulin,leptin)of control and treatment group were measured on day 1,2,4,6,8,10 during supplementation.The numbers of the follicles were counted in categories of small follicle(≤3.0 mm),medium follicle(3.0-5.0 mm) and large follicles(≥5.0 mm) and the follicular fluid and granulose cells were collected respectively,the expression of mRNA of OB-R and IGF-1 were detected in follicular granulose cells by real-time PCR on day 13,14,and 15 of six ewes respectively.Compared with control,the numbers of medium follicles and large follicles significantly increased (P<0.01),whereas the numbers of small follicles significantly decreased(P<0.01)in treatment group.Meanwhile,the mean concentrations of glucose(P<0.01),insulin(P<0.01)and leptin(P<0.05)in plasma and large follicles increased significantly.The expression level of OB-R and IGF-1 increased significantly in granulose cells of medium and large follicles.The short-term nutritional supplementation during luteal phase improves the numbers and dominance of large follicles through increasing the levels of glucose,insulin,leptin in plasma and the expression of OB-Rand IGF-1 mRNA.
The study was to evaluate the effects of short-term nutritional supplementation on follicular development during luteal phase in ewes.60 Dorset×Han crossbred ewes were divided randomly into two groups of control(n=30) and treatment(n=30).All ewes were fed on basal diet(DE 11.72 MJ/d;DP 79.71 g/d) originally.Then ewes was treated to oestrus synchronization(3 d after intramuscularly injection of PG 0.1 mg,CIDR for 12 d,then PG injection again for the same dose on the day of removal of CIDR).From day 2 of CIDR embedment,a high-energy supplement diet(DE18.75 MJ/d;DP 108.44 g/d) was fed to the treatment group for 10 d.Plasma metabolites(glucose,insulin,leptin)of control and treatment group were measured on day 1,2,4,6,8,10 during supplementation.The numbers of the follicles were counted in categories of small follicle(≤3.0 mm),medium follicle(3.0-5.0 mm) and large follicles(≥5.0 mm) and the follicular fluid and granulose cells were collected respectively,the expression of mRNA of OB-R and IGF-1 were detected in follicular granulose cells by real-time PCR on day 13,14,and 15 of six ewes respectively.Compared with control,the numbers of medium follicles and large follicles significantly increased (P<0.01),whereas the numbers of small follicles significantly decreased(P<0.01)in treatment group.Meanwhile,the mean concentrations of glucose(P<0.01),insulin(P<0.01)and leptin(P<0.05)in plasma and large follicles increased significantly.The expression level of OB-R and IGF-1 increased significantly in granulose cells of medium and large follicles.The short-term nutritional supplementation during luteal phase improves the numbers and dominance of large follicles through increasing the levels of glucose,insulin,leptin in plasma and the expression of OB-Rand IGF-1 mRNA.
引文
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[28] ROBERT J,BARNEVELDA V.Understanding the nutritional chemistry of lupin (Lupinus spp.) seed to improve livestock production efficiency[J].Nutr Res Rev,1999,12(2):203-230.
[29] HASHEM N M,El-ZARKOUNY S Z.Effect of short-term supplementation with rumen-protected fat during the late luteal phase on reproduction and metabolism of ewes[J].J Anim Physiol Anim Nutr (Berl),2014,98(1):65-71.
[30] BERLINGUER F,GONZALEZ-BULNES A,CONTRERAS-SOLIS I,et al.Glucogenic supply increases oocyte developmental competence in sheep[J].Reprod Fertil Dev,2012,24(8):1055-1062.
[31] PORCU C,PASCIU V,SUCCU S,et al.Glucogenic treatment creates an optimal metabolic milieu for the conception period in ewes[J].Domest Anim Endocrinol,2017,59:105-115.
[32] WILLIAMS S A,BLACHE D,MARTIN G B,et al.Effect of nutritional supplementation on quantities of glucose transporters 1 and 4 in sheep granulosa and theca cells[J].Reproduction,2001,122(6):947-956.
[33] SCARAMUZZI R,CAMPBELL B,DOWNING J,et al.A review of the effects of supplementary nutrition in the ewe on the concentrations of reproductive and metabolic hormones and the mechanisms that regulate folliculogenesis and ovulation rate[J].Reprod Nutr Dev,2006,46(4):339-354.
[34] BROGAN R S,MACGIBENY M,MIX S,et al.Dynamics of intra-follicular glucose during luteinization of macaque ovarian follicles[J].Mol Cell Endocrinol,2011,332(1/2):189-195.
[35] 应诗家.黄体期不同饲喂量对湖羊卵泡发育、血液理化指标和卵泡内微环境的影响[D].南京:南京农业大学,2012.
[36] SMOLINSKA N,KAMINSKI T,SIAWRYS G,et al.Expression of leptin and its receptor genes in the ovarian follicles of cycling and early pregnant pigs[J].Animal,2013,7:109-117.
[37] DEL B B,FRANCI C R.Estrogen-dependent post-translational change in the nitric oxide system May mediate the leptin action on LH and prolactin secretion[J].Brain Res,2015,1604:62-73.
[38] BILBAO M G,DI YORIO M P,FALETTI A G.Different levels of leptin regulate different target enzymes involved in progesterone synthesis[J].Fertil Steril,2013,99(5):1460-1466.
[39] DAYI A,BEDIZ C S,MUSAL B,et al.Comparison of leptin levels in serum and follicular fluid during the oestrous cycle in cows[J].Acta Vet Hung,2005,53(4):457-467.
[40] SARKAR M,SCHILFFARTH S,SCHAMS D,et al.The expression of leptin and its receptor during different physiological stages in the bovine ovary[J].Mol Reprod Dev,2010,77(2):174-181.
[41] SEYYED J T,ABBAS P.Sheep oocyte expresses leptin and functional leptin receptor mRNA[J].Asian Pacific J Reprod,2016,5(5):395-399.
[42] BATISTA A M,SILVA D M,REGO M J,et al.The expression and localization of leptin and its receptor in goat ovarian follicles[J].Anim Reprod Sci,2013,141(3/4):142-147.
[43] ZIEBA D A,SZCZESNA M,KLOCEKGORAKA B,et al.Leptin as a nutritional signal regulation appetite and reproductive processes in seasonally-breeding ruminants[J].J Physiol Pharmacol,2008,59(9):7-18.
[44] D?SOUZA A M,NEUMANN U H,GLAVAS M M,et al.The glucoregulatory action of leptin[J].Mol Metab,2017,6(9):1052-1065.
[45] SOMCHIT-ASSAVACHEEP A,CAMPBELL B K,KHALID M, et al.The effect of short-term nutritional supplementation of ewes with lupin grain (Lupinus luteus) on folliculogenesis,the concentrations of hormones and glucose in plasma and follicular fluid and the follicular levels of P450 aromatase and IRS-1,-2 and -4[J].Reproduction,2013,145(4):319-333.
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[2] BUTLER W R.Nutritional interactions with reproductive performance in dairy cattle[J].Anim Reprod Sci,2000,60 (4):449-459.
[3] KOSIOR-KORZECKA U,BOBOWIEC R,LIPECKA C.Fasting-induced changes in ovulation rate,plasma leptin,gonadotropins,GH,IGF-I and insulin concentrations during oestrus in ewes[J].J Vet Med,2006,53(1):5-11.
[4] 罗霏菲.黄体期高低饲喂水平影响湖羊卵泡发育的表达谱分析[D].南京:南京农业大学,2014.
[5] BOLAND M P,LONERGAN P,O'CALLAGHAN D.Effect of nutrition on endocrine parameters,ovarian physiology,and oocyte and embryo development[J].Theriogenology,2001,55(6):1323-1340.
[6] ROBINSON R S,PUSHPAKUMARA P G,CHENG Z,et al.Effects of dietary polyunsaturated fatty acids on ovarian and uterine function in lactating dairy cows[J].Reproduction,2002,124(1):119-131.
[7] NOGUEIRA D M,CAVALIERI J,FITZPATRICK L A,et al.Effect of hormonal synchronisation and/or short-term supplementation with maize on follicular dynamics and hormone profiles in goats during the non-breeding season[J].Anim Reprod Sci,2016,171:87-97.
[8] ZHANG S,BLACHE D,BLACKBERRY M A,et al.Dynamics of the responses in secretion of luteinising hormone,leptin and insulin following an acute increase in nutrition in mature male sheep[J].Reproduction,2004,16(8):823-829.
[9] VINOLES C,FORSBERG M,BANCHERO G,et al.Ovarian follicular dynamics and endocrine profiles in Polwarth ewes with high and low bodycondition[J].Anim Sci,2002,74(3):535-545.
[10] SCARAMUZZI R J,CAMPBELL B K,SOUZA C J H,et al.Glucose uptake and lactate production by the auto transplanted ovary of the ewe during the luteal and follicular phases of the oestrous cycle[J].Theriogenology,2010,73(8):1061-1067.
[11] AHRENS K,MUMFORD S L,SCHLIEP K C,et al.Serum leptin levels and reproductive function during the menstrual cycle[J].Am J Obstet Gynecol,2014,210(3):1-9.
[12] VINOLES C,FORSBERG M,MARTIN G B,et al.Short-term nutritional supplementation of ewes in low body condition affects follicle development due to an increase in glucose and metabolic hormones[J].Reproduction,2005,129(3):299-309.
[13] KOSIOR-KORZECKA U,BOBOWIEC R.Changes in the level of endogenous leptin,fsh,17β-oestradiol and metabolites during lupin-induced increase in ovulation rate in ewes[J].J Vet Med A Physiol Pathol Clin Med,2003,50(7):343-349.
[14] ROLAND A V,MOENTER S M.Regulation of gonadotropin-releasing hormone neurons by glucose[J].Trends Endocrinol Metab,2011,22(11):443-449.
[15] PORETSKY L,CATADO N A,ROSENWAKS Z,et al.The insulin-related ovarian regulatory system in health and disease[J].Endocr Rev,1999,20(4):535-582.
[16] BURCELIN R,THORENS B,GLAUSER M,et al.Gonadotropin-releasing hormone secretion form hypothalamic neurons:stimulation by insulin and potentiation by leptin[J].Endocrinology,2003,144(10):4484-4491.
[17] O?CALLAGHAN D,BOLAND M P.Nutritional effects on ovulation,embryo development and the establishment of pregnancy in ruminants[J].Anim Sci,1999,68(2):299-314.
[18] SULLIVAN R R,FARIS B R,EBORN D,et al.Follicular expression of follicle stimulating hormone receptor variants in the ewe[J].Reprod Biol Endocrinol,2013,11(1):113-120.
[19] NOGUEIRA D M,ESHTAEBA A,CAVALIERI J,et al.Short-term supplementation with maize increases ovulation rate in goats when dietary metabolizable energy provides requirements for both maintenance and 1.5 times maintenance[J].Theriogenology,2017,89:97-105.
[20] 谷振慧.生绒期和品种对山羊绒毛生长相关激素分泌及基因表达的影响[D].河北保定:河北农业大学,2015.
[21] VLCKOVA R,POSIVAK J,VALOCKY I,et al.The effect of short-term lupin (Lupinus angustifolius) feed supplementation on serum steroid hormones,insulin-like growth factor I,and ovarian follicular development and atresia in Merino ewes[J].Turkish J Vet Anim Sci,2014,38(6):686-692.
[22] HABIBIZAD J,RIASI A,KOHRAM H,et al.Effect of long-term or short-term supplementation of high energy or high energy-protein diets on ovarian follicles and blood metabolites and hormones in ewes[J].Small Ruminant Res,2015,132:37-43.
[23] GALLET C,DUPONT J,BRUCE K,et al.The infusion of glucose in ewes during the luteal phase increases the number of follicles but reduces oestradiol production and some correlates of metabolic function in the large follicles[J].Anim Reprod Sci,2011,127(3/4):154-163.
[24] LETELIER C,GONZALEZBULNES A,HERVE M,et al.Enhancement of ovulatory follicle development in Maiden sheep by short-term supplementation with steam-flaked corn[J].Reprod Domest Anim,2008,43(2):222-227.
[25] ZABULI J,TANAKA T,LU W,et al.Intermittent nutritional stimulus by short-term treatment of high-energy diet promotes ovarian performance together with increases in blood levels of glucose and insulin in cycling goats[J].Anim Reprod Sci,2010,122(3/4):288-293.
[26] SOMCHIT A,CAMPBELL B K,KHALID M,et al.The effect of short-term nutritional supplementation of ewes with lupin grain (Lupinus luteus),during the luteal phase of the estrous cycle on the number of ovarian follicles and the concentrations of hormones and glucose in plasma and follicular fluid[J].Theriogenology,2007,68(7):1037-1046.
[27] NOGUEIRA D M,CAVALIERI J,FITZPATRICK L A,et al.Effect of hormonal synchronisation and/or short-term supplementa- tion with maize on follicular dynamics and hormone profiles in goats during the non-breeding season[J].Anim Reprod Sci,2016,171:87-97.
[28] ROBERT J,BARNEVELDA V.Understanding the nutritional chemistry of lupin (Lupinus spp.) seed to improve livestock production efficiency[J].Nutr Res Rev,1999,12(2):203-230.
[29] HASHEM N M,El-ZARKOUNY S Z.Effect of short-term supplementation with rumen-protected fat during the late luteal phase on reproduction and metabolism of ewes[J].J Anim Physiol Anim Nutr (Berl),2014,98(1):65-71.
[30] BERLINGUER F,GONZALEZ-BULNES A,CONTRERAS-SOLIS I,et al.Glucogenic supply increases oocyte developmental competence in sheep[J].Reprod Fertil Dev,2012,24(8):1055-1062.
[31] PORCU C,PASCIU V,SUCCU S,et al.Glucogenic treatment creates an optimal metabolic milieu for the conception period in ewes[J].Domest Anim Endocrinol,2017,59:105-115.
[32] WILLIAMS S A,BLACHE D,MARTIN G B,et al.Effect of nutritional supplementation on quantities of glucose transporters 1 and 4 in sheep granulosa and theca cells[J].Reproduction,2001,122(6):947-956.
[33] SCARAMUZZI R,CAMPBELL B,DOWNING J,et al.A review of the effects of supplementary nutrition in the ewe on the concentrations of reproductive and metabolic hormones and the mechanisms that regulate folliculogenesis and ovulation rate[J].Reprod Nutr Dev,2006,46(4):339-354.
[34] BROGAN R S,MACGIBENY M,MIX S,et al.Dynamics of intra-follicular glucose during luteinization of macaque ovarian follicles[J].Mol Cell Endocrinol,2011,332(1/2):189-195.
[35] 应诗家.黄体期不同饲喂量对湖羊卵泡发育、血液理化指标和卵泡内微环境的影响[D].南京:南京农业大学,2012.
[36] SMOLINSKA N,KAMINSKI T,SIAWRYS G,et al.Expression of leptin and its receptor genes in the ovarian follicles of cycling and early pregnant pigs[J].Animal,2013,7:109-117.
[37] DEL B B,FRANCI C R.Estrogen-dependent post-translational change in the nitric oxide system May mediate the leptin action on LH and prolactin secretion[J].Brain Res,2015,1604:62-73.
[38] BILBAO M G,DI YORIO M P,FALETTI A G.Different levels of leptin regulate different target enzymes involved in progesterone synthesis[J].Fertil Steril,2013,99(5):1460-1466.
[39] DAYI A,BEDIZ C S,MUSAL B,et al.Comparison of leptin levels in serum and follicular fluid during the oestrous cycle in cows[J].Acta Vet Hung,2005,53(4):457-467.
[40] SARKAR M,SCHILFFARTH S,SCHAMS D,et al.The expression of leptin and its receptor during different physiological stages in the bovine ovary[J].Mol Reprod Dev,2010,77(2):174-181.
[41] SEYYED J T,ABBAS P.Sheep oocyte expresses leptin and functional leptin receptor mRNA[J].Asian Pacific J Reprod,2016,5(5):395-399.
[42] BATISTA A M,SILVA D M,REGO M J,et al.The expression and localization of leptin and its receptor in goat ovarian follicles[J].Anim Reprod Sci,2013,141(3/4):142-147.
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