正交优化知母黄酮超声提取工艺及抗氧化研究
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摘要
[目的]研究知母根茎总黄酮的超声波提取工艺并对体外抗氧化性进行测定.[方法]考察以乙醇为溶剂时液料比、超声温度、超声功率、超声时间对提取率的影响,并用正交法筛选最佳工艺.[结果]实验表明:各因素对知母总黄酮萃取得率的影响由大到小依次为液料比、超声功率、超声温度、超声时间;最佳提取工艺条件为:液料比40∶1(mL/g)、超声温度60℃、超声时间45min、超声功率150 W,此条件下总黄酮提取率高达1.53%.[结论]由自由基清除试验可知,知母总黄酮抗氧化能力与其浓度呈正向量效关系,对·OH和DPPH·的清除效率分别可达60.2%,76.5%,显示其具较强的体外抗氧化能力.
[Objective]The ultrasonic extraction technology and antioxidant activity of total flavonoids from Anemarrhena were optimized.[Methods]The root and stem of Anemarrhena was used as raw material,ethanol as extractant,the ratio of material to liquid,ultrasonic time,ultrasonic power,ultrasonic temperature as the investigation factor,the extraction rate of total flavonoids as the index,screen the optimal conditions by single factor experiments and orthogonal test.[Results]The factors affect the extraction rate were:the ratio of material to liquid> power>temperature>ultrasonic time,and the optimum extraction conditions of total flavonoids are as follows:the best ratio ofliquid l to materia is 40:1(mL/g),the best ultrasonic time is 45 min,the best ultrasonic power is 150 W,the best ultrasonic temperature is 60℃.Under these conditions,the extraction rate of total flavonoids from Anemarrhenareached 1.53%.[Conclusion]The antioxidant activity was verified by free radical scavenging experiments.It was found that the antioxidant capacity of total flavonoids from Anemarrhena Rhizome was positively correlated with its concentration,and the scavenge rate of free radical such as DPPH·and· OH can achieve 76.5% and 60.2%,so it has strong antioxidant properties.
[Objective]The ultrasonic extraction technology and antioxidant activity of total flavonoids from Anemarrhena were optimized.[Methods]The root and stem of Anemarrhena was used as raw material,ethanol as extractant,the ratio of material to liquid,ultrasonic time,ultrasonic power,ultrasonic temperature as the investigation factor,the extraction rate of total flavonoids as the index,screen the optimal conditions by single factor experiments and orthogonal test.[Results]The factors affect the extraction rate were:the ratio of material to liquid> power>temperature>ultrasonic time,and the optimum extraction conditions of total flavonoids are as follows:the best ratio ofliquid l to materia is 40:1(mL/g),the best ultrasonic time is 45 min,the best ultrasonic power is 150 W,the best ultrasonic temperature is 60℃.Under these conditions,the extraction rate of total flavonoids from Anemarrhenareached 1.53%.[Conclusion]The antioxidant activity was verified by free radical scavenging experiments.It was found that the antioxidant capacity of total flavonoids from Anemarrhena Rhizome was positively correlated with its concentration,and the scavenge rate of free radical such as DPPH·and· OH can achieve 76.5% and 60.2%,so it has strong antioxidant properties.
引文
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[13]杨喆,万山,张乔会,等.响应面法优化山杏核壳总黄酮提取工艺及其抗氧化性的研究[J].食品工业科技,2015,36(6):279-284
[14]孟永海,怀雪,翟春梅,王知斌,等.刺玫果提取物总黄酮含量以及体外抗氧化活性的测定[J].化学工程师,2018,32(11):26-28
[15]曹剑锋,刘其高,蒋小飞,等.阴地蕨总黄酮的提取及抗氧化活性研究[J].江苏农业科学,2018,46(21):228-231
[2]倪梁红,秦民坚.知母资源化学及药理研究进展[J].中国野生植物资源,2005,24(4):16
[3]徐冬英,谢崇源,韦衮政.知母的研究进展[J].广西中医学院学报,1999,16(4):93
[4]张颖,陈朝,陈宇霞,等.知母及含知母复方的抗抑郁研究现状[J].环球中医药,2016,9(3):377-380
[5]方菊.竹叶黄酮的提取分离有抑菌效果研究[D].合肥:合肥工业大学,2012
[6]陈卫东,张友胜,肖更生,等.金柚皮活性成分的提取与蜜饯产品的加工[J].广东农业科学,2004,(6):76-77
[7]杨晓泉,张海德,李琳.柚皮黄酮类抗氧化物质的纯化及其降血脂作用的研究[J].营养学报,2004,(5):378-381
[8]李春美,杜靖,谢笔钧.柚皮提取物的抑菌作用[J].食品与发酵工业,2004,30(1):38-41
[9]WANG A M,ZHANG F K,HUANG L F,et al.New progress in biocatalysis and biotransformation of flavonoids[J].Journal of Medicinal Plants Research,2010,4(10):847-856
[10]禚同友.超声波法提取凤尾竹叶黄酮工艺研究[J].食品研究与开发,2015,36(19):59-61
[11]秦明有.竹叶黄酮的超声提取工艺研究[J].江西饲料,2017(6):1-4
[12]周宁,赵晓璐,谢庆武.超声波辅助提取木薯叶总黄酮及抗氧化性研究[J].粮食与油脂,2016,29(10):21-24
[13]杨喆,万山,张乔会,等.响应面法优化山杏核壳总黄酮提取工艺及其抗氧化性的研究[J].食品工业科技,2015,36(6):279-284
[14]孟永海,怀雪,翟春梅,王知斌,等.刺玫果提取物总黄酮含量以及体外抗氧化活性的测定[J].化学工程师,2018,32(11):26-28
[15]曹剑锋,刘其高,蒋小飞,等.阴地蕨总黄酮的提取及抗氧化活性研究[J].江苏农业科学,2018,46(21):228-231
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