激光拉曼光谱无损分析浙贝母
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摘要
通过采集浙江省5个浙贝母主产区不同生产基地的新鲜样品,获取浙贝母激光拉曼光谱的图谱,并进行特征峰分析和一阶导数分析。结果表明,浙贝母在875、912、965、1 153、1 312、1 410、1 482、1 598、1 641 cm~(-1)等位移处出现9处明显的特征峰,并初步判定了这些特征峰的归属。研究结果表明,采用拉曼光谱进行农产品分析具有无损、快速、简便的特点,研究结果获取的9个特征峰可以作为鉴别浙贝母真伪、产地、溯源的手段。
Fresh samples of Thunberg Fritillary Bulb from 5 main planting areas in Zhejiang Province were collected and the laser Raman spectroscopy was used to analyze and identify the samples. The results were used for the characteristic peak and first-order derivative analysis. The results showed that several characteristic strong peaks could be found at 875,912,965,1 153,1 312,1 410,1 482,1 598,1 641 cm~(-1) shift,and the affiliation of these peaks was identified. The results showed that Raman spectroscopic has the advantages of nondestructive,fast and simple,and the identified 9 peaks would be realized as the measures to distinguish the truth,origin area and traceability of Thunberg Fritillary Bulb.
Fresh samples of Thunberg Fritillary Bulb from 5 main planting areas in Zhejiang Province were collected and the laser Raman spectroscopy was used to analyze and identify the samples. The results were used for the characteristic peak and first-order derivative analysis. The results showed that several characteristic strong peaks could be found at 875,912,965,1 153,1 312,1 410,1 482,1 598,1 641 cm~(-1) shift,and the affiliation of these peaks was identified. The results showed that Raman spectroscopic has the advantages of nondestructive,fast and simple,and the identified 9 peaks would be realized as the measures to distinguish the truth,origin area and traceability of Thunberg Fritillary Bulb.
引文
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[10]郭鹏程,薛靖虹,陈相柏.激光拉曼光谱检测灵芝孢子油[J].光谱学与光谱分析,2018,38(3):1129-1132.GUO P C,XUE J H,CHEN X B.Raman spectroscopy study of Ganoderma spore oil[J].Spectroscopy and Spectral Analysis,2018,38(3):1129-1132.(in Chinese with English abstract)
[11]陈珊,李晓宁,梁逸曾,等.拉曼光谱的荧光背景扣除及其用于药物聚类分析[J].光谱学与光谱分析,2010,30(8):2157-2160.CHEN S,LI X N,LIANG Y Z,et al.Raman spectroscopy fluorescene background correction and its application in clustering analysis of medicines[J].Spectroscopy and Spectral Analysis,2010,30(8):2157-2160.(in Chinese with English abstract)
[12]赵耀东,杜伟锋,王胜波,等.基于近红外光谱的浙贝母中贝母素甲和贝母素乙含量快速检测方法的建立[J].中华中医药学刊,2013,31(4):756-757.ZHAO Y D,DU W F,WANG S B,et al.Rapid detection of peimine and peiminine in Bulbus Fritillariae Thunbergi by near infrared spectroscopy[J].China Archives of Traditional Chinese Medicine,2013,31(4):756-757.(in Chinese with English abstract)
[2]ZHAO J,HUI H,MCLEAN D I,et al.Automated autofluorescence background subtraction algorithm for biomedical Raman spectroscopy[J].Applied Spectroscopy,2007,61(11):1225-1232.
[3]YANG D,YING Y.Applications of Raman spectroscopy in agricultural products and food analysis:a review[J].Applied Spectroscopy Reviews,2011,46(7):539-560.
[4]王文娜,陈地灵,朱梅芳,等.激光拉曼光谱法无损分析鉴别川贝母[J].光谱学与光谱分析,2013,33(8):2109-2111.WANG W N,CHEN D L,ZHU M F,et al.The analysis and identification of Fritillaria cirrhosa by Raman spectra[J].Spectroscopy and Spectral Analysis,2013,33(8):2109-2111.(in Chinese with English abstract)
[5]林文硕,陈荣,李永增,等.山药近红外拉曼光谱分析[J].光谱学与光谱分析,2008,28(5):1095-1097.LIN W S,CHEN R,LI Y Z,et al.Near infrared Raman spectra analysis of Rhizoma dioscoreae[J].Spectroscopy and Spectral Analysis,2008,28(5):1095-1097.(in Chinese with English abstract)
[6]DOLLISH F R,FATELEY W G,BENTLEY F F.有机化合物的特征拉曼频率[M].朱自莹,译.北京:中国化学会,1980:8.
[7]许以明.拉曼光谱及其在结构生物学中的应用[M].北京:化学工业出版社,2005:3.
[8]刘宸,黄文倩,王庆艳,等.拉曼光谱在食品无损检测中的应用[J].食品安全质量检测学报,2015,6(8):2981-2986.LIU C,HUANG W Q,WANG Q Y,et al.Application of Raman spectroscopy technique in food non-destructive determination[J].Journal of Food Safety&Quality,2015,6(8):2981-2986.(in Chinese with English abstract)
[9]王利军,王红,谢乐,等.拉曼光谱快速鉴别花生油掺棕榈油的研究[J].中国油料作物学报,2013,35(5):604-607.WANG L J,WANG H,XIE L,et al.Rapid authentication of palm oil in pure peanut oil by Raman spectroscopy[J].Chinese Journal of Oil Crop Sciences,2013,35(5):604-607.(in Chinese with English abstract)
[10]郭鹏程,薛靖虹,陈相柏.激光拉曼光谱检测灵芝孢子油[J].光谱学与光谱分析,2018,38(3):1129-1132.GUO P C,XUE J H,CHEN X B.Raman spectroscopy study of Ganoderma spore oil[J].Spectroscopy and Spectral Analysis,2018,38(3):1129-1132.(in Chinese with English abstract)
[11]陈珊,李晓宁,梁逸曾,等.拉曼光谱的荧光背景扣除及其用于药物聚类分析[J].光谱学与光谱分析,2010,30(8):2157-2160.CHEN S,LI X N,LIANG Y Z,et al.Raman spectroscopy fluorescene background correction and its application in clustering analysis of medicines[J].Spectroscopy and Spectral Analysis,2010,30(8):2157-2160.(in Chinese with English abstract)
[12]赵耀东,杜伟锋,王胜波,等.基于近红外光谱的浙贝母中贝母素甲和贝母素乙含量快速检测方法的建立[J].中华中医药学刊,2013,31(4):756-757.ZHAO Y D,DU W F,WANG S B,et al.Rapid detection of peimine and peiminine in Bulbus Fritillariae Thunbergi by near infrared spectroscopy[J].China Archives of Traditional Chinese Medicine,2013,31(4):756-757.(in Chinese with English abstract)