中药汤剂包材中邻苯二甲酸二辛酯迁移的检测
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摘要
目的建立一种有效的中药汤剂包材中邻苯二甲酸二辛酯迁移量的检测方法。方法采用COSMOSIL5C18-AR-Ⅱ(4.6 ID×150 mm)C18色谱柱分离,流动相为乙腈、甲醇、水(90∶10∶0.5),进样量,对照品20μl样品40μl,流速为1.0 ml/min,柱温为30℃,检测波长224 nm。检测灌装后邻苯二甲酸二辛酯的迁移量。结果质量浓度与其峰面积具有良好的线性关系(r=0.999 49),邻苯二甲酸二辛酯成分在15 min内得到了较好分离。检出限为1.534 566 mg/L,平均加标回收率为101.407 0%,仪器精密度相对标准偏差为0.501%,方法相对标准偏差2.101%。结论本方法灵敏、简便,适用于中药汤剂包材中邻苯二甲酸二辛酯迁移量的检测。
Objective To establish an effective detection method of dioctyl phthalate( DEHP)migration contained in Chinese decoction packing materials. Methods COSM OSIL 5 C18-AR-Ⅱ(4. 6 ID× 150 mm) C18 chromatogram column separation w as performed w ith mobile phase of acetonitrile,methanol and w ater(90 ∶ 10 ∶ 0. 5),sample size of 20 μl( for control) and 40 μl( for sample),flow rate of 1. 0 ml/min,column temperature of 30 ℃ and detection w avelength of 224 nm. The amount of DEHP migration after filling w as detected. Results M ass concentration and peak area from each component had good linear relationship(r = 0. 999 49),and the DEPH component w as separated w ithin15 min. Detection limit w as 1. 534 566 mg/L w ith average recovery of 101. 407 0%. The instrument precision relative standard deviation w as 0. 501%,method relative standard deviation( RSD) w as2. 101%. Conclusion The established method is sensitive and convenient,suitable for detection of dioctyl phthalate migration contained in Chinese decoction packing materials.
Objective To establish an effective detection method of dioctyl phthalate( DEHP)migration contained in Chinese decoction packing materials. Methods COSM OSIL 5 C18-AR-Ⅱ(4. 6 ID× 150 mm) C18 chromatogram column separation w as performed w ith mobile phase of acetonitrile,methanol and w ater(90 ∶ 10 ∶ 0. 5),sample size of 20 μl( for control) and 40 μl( for sample),flow rate of 1. 0 ml/min,column temperature of 30 ℃ and detection w avelength of 224 nm. The amount of DEHP migration after filling w as detected. Results M ass concentration and peak area from each component had good linear relationship(r = 0. 999 49),and the DEPH component w as separated w ithin15 min. Detection limit w as 1. 534 566 mg/L w ith average recovery of 101. 407 0%. The instrument precision relative standard deviation w as 0. 501%,method relative standard deviation( RSD) w as2. 101%. Conclusion The established method is sensitive and convenient,suitable for detection of dioctyl phthalate migration contained in Chinese decoction packing materials.
引文
[1]董瑞娜,戴红.DEHP对雄性生殖系统毒性的研究进展[J].世界最新医学信息文摘,2016,16(90):57-59.
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[6]张双灵,徐仰丽,王世清.食品塑料袋中DEHP气相色谱检测方法的建立[J].食品科学,2007,28(8):341-344.
[7]蔡智鸣,史馨,王枫华,等.食品中酞酸酯类环境污染物的GC-MS测定[J].同济大学学报(医学版),2005,26(3):1-3.
[2]蒋磊,徐维平,芮贝贝,等.邻苯二甲酸二乙基己酯体内代谢和毒性机制研究进展[J].中国药业,2015,24(5):1-3.
[3]刘萌,倪赞,蒋惠娣.HF-LPME-GC测定PVC输液管路中6种邻苯二甲酸酯的迁移[J].药物分析杂志,2014,34(12):2210-2216.
[4]付忻,冯铁男,张露丹,等.京沪穗三城市空气质量变化及其与肺癌发病的关系[J].同济大学学报(医学版),2015,36(5):96-100.
[5]SARATH JOSH M K,PRADEEP S,BALACHANDRAN S,et al.Temperature-and solvent-dependent migrations of di(2-ethylhexyl)phthalate,the hazardous plasticizer from commercial PVC blood storage bag[J].J Polym Res,2012,19(7):9915.
[6]张双灵,徐仰丽,王世清.食品塑料袋中DEHP气相色谱检测方法的建立[J].食品科学,2007,28(8):341-344.
[7]蔡智鸣,史馨,王枫华,等.食品中酞酸酯类环境污染物的GC-MS测定[J].同济大学学报(医学版),2005,26(3):1-3.