15个饲用燕麦品种的遗传变异及亲缘关系分析
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摘要
采用酸性聚丙烯酰胺凝胶电泳(A-PAGE)对15个燕麦品种种子醇溶蛋白进行遗传变异分析。结果表明供试的15个品种共扩增出18条带,多态率位点率为100%,条带主要分布于α和β区域。供试品种的遗传相似系数变化为0.143～1.000,平均值为0.527,表明供试的品种间遗传变异较大。除青海444和青燕1号外,醇溶蛋白电泳图谱能把不同的品种进行分离。聚类等分析结果将供试品种分为6大类,砂燕麦、阿坝、青引1号与其他品种关系较远,其中青海甜燕麦、青燕1号和青海444的关系较近。
The acid polyacrylamide gel electrophoresis(A-PAGE)was used to detect the gliadin genetic polymorphism among 15 oat varieties.A total of 18 bands were detected in the 15 oat varieties,100% were polymorphic bands,which located inαandβzone.The DICE genetic similarity coefficient of the tested varieties ranged from 0.143-1.000,and the average coefficient was 0.527,which showed that there was rich genetic polymorphism among the tested oat varieties.All the test varieties except Qinghai 444 and Qingyan No.1 could be separated by the gliadin map.Based on the UPGMA cluster analysis,the 15 varieties were clustered into six groups on dendrogram.The Strigosa,A'ba and Qingyin No.1 oat showed the specificity in their genetic background and showed a rather far relationship among others.There was a rather close relationship among Qinghai 444,Qingyan No.1 and Qinghai sweet oat.The result in the study revealed the genetic variation and kinship amongthetestedvarietiesI.twouldprovide the useful information for the oat varieties utilization and germplasm creation in the further study.
The acid polyacrylamide gel electrophoresis(A-PAGE)was used to detect the gliadin genetic polymorphism among 15 oat varieties.A total of 18 bands were detected in the 15 oat varieties,100% were polymorphic bands,which located inαandβzone.The DICE genetic similarity coefficient of the tested varieties ranged from 0.143-1.000,and the average coefficient was 0.527,which showed that there was rich genetic polymorphism among the tested oat varieties.All the test varieties except Qinghai 444 and Qingyan No.1 could be separated by the gliadin map.Based on the UPGMA cluster analysis,the 15 varieties were clustered into six groups on dendrogram.The Strigosa,A'ba and Qingyin No.1 oat showed the specificity in their genetic background and showed a rather far relationship among others.There was a rather close relationship among Qinghai 444,Qingyan No.1 and Qinghai sweet oat.The result in the study revealed the genetic variation and kinship amongthetestedvarietiesI.twouldprovide the useful information for the oat varieties utilization and germplasm creation in the further study.
引文
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[14]罗桂花,马继雄,花立民.青海燕麦农家品种的醇溶蛋白电泳分析(简报)[J].草业学报,2004,13(2):112-114.
[15]刘敏轩,王赟文,韩建国,等.饲用燕麦(Avena sativa L.)品种的种子醇溶蛋白超薄层等电聚焦电泳快速鉴定[J].种子,2006,25(9):4-8.
[16]彭远英,颜红海,郭来春,等.燕麦属不同倍性种质资源抗旱性评价及筛选[J].生态学报,2011,31(9):2478-2491.
[2]任长忠,胡跃高.中国燕麦学[M].北京:中国农业出版社,2013,11.
[3]周青平.高原燕麦的栽培与管理[M].南京:江苏凤凰科学技术出版社,2014,11.
[4]刘欢,张新全,马啸,等.基于荧光检测技术的多花黑麦草EST-SSR指纹图谱构建[J].中国农业科学,2017,50(3):437-450.
[5]蒋林峰,张新全,黄琳凯,等.中国鸭茅主栽品种DNA指纹图谱构建[J].植物遗传资源学报,2014,15(3):604-614.
[6]郎明林,卢少源,张荣芝.中国北方冬麦区主栽品种醇溶蛋白指纹图谱数据库的建立[J].中国农业科学,2002,35(3):238-244.
[7]孙正文,匡猛,马峙英,等.利用Cotton SNP 63K芯片构建棉花品种的指纹图谱[J].中国农业科学,2017,50(24):4692-4704.
[8]齐冰洁,刘景辉,高聚林,等.燕麦种质资源醇溶蛋白遗传多样性研究[J].麦类作物学报,2010,30(3):427-430.
[9]刘欢,幕平,赵桂琴.基于AFLP的燕麦遗传多样性研究[J].草业学报,2008,17(6):121-127.
[10]张曼,田娟,刘宏魁,等.燕麦光照不敏感基因SSR分子标记的引物筛选[J].分子植物育种,2017,14(3):974-978.
[11]Draper S R.ISTA variety committee report of the working group for biochemical tests for cultivar identification 1983-1986[J].Seed Science and Technology,1987,15:431-434.
[12]Rohlf F J.NTSYS-pc:Numerical Taxonomy and Multivariate Analysis System,Version 2.0[M].New York:Exter Software,1994.
[13]张学勇,杨欣明,董玉琛.醇溶蛋白电泳在小麦种质资源遗传分析中的应用[J].中国农业科学,1995,28(4):25-32.
[14]罗桂花,马继雄,花立民.青海燕麦农家品种的醇溶蛋白电泳分析(简报)[J].草业学报,2004,13(2):112-114.
[15]刘敏轩,王赟文,韩建国,等.饲用燕麦(Avena sativa L.)品种的种子醇溶蛋白超薄层等电聚焦电泳快速鉴定[J].种子,2006,25(9):4-8.
[16]彭远英,颜红海,郭来春,等.燕麦属不同倍性种质资源抗旱性评价及筛选[J].生态学报,2011,31(9):2478-2491.