基于纳米探针和石墨烯电极技术的ATP生物发光检测系统
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摘要
由细菌引起的食品安全问题越来越受到人们的重视,使得快速细菌检测技术成为一个重要课题.设计了一种基于纳米探针技术、石墨烯透明电极(GTE)技术和三磷酸腺苷(ATP)发光技术的生物检测系统,能够快速准确地检测食品中的细菌.实验结果表明该方法与传统检测方法具有很好的相关性,线性相关度可达0.972,检测时间控制在20min内.检测范围在102-106 CFU/mL.此外通过检测不同污染物测得该系统具有令人满意的性能.可以满足现场快速检测的要求.
Food safety issues caused by the bacteria have drawn people′s attention increasingly,fast and accurate detection technology becomes an urgent research topic.The biological detection system based on nano-probe technology,graphene transparent electrode(GTE)technology and adenosine triphosphate(ATP)luminescence technology is designed in this paper,it can detect the bacteria of food automatically and rapidly.The results indicate that there is a good correlation between the rapid detecting method and the other conventional approaches.the linear coefficient can be up to 0.972,and the detecting time can be controlled within twenty minutes.The detectable bacteria concentration range reaches 102-106 CFU/mL.Moreover,a series of curves obtained by measurements for different polluted foods show that the system has a satisfactory performance.It is sufficient to meet the requirements of on-site rapid detection.
Food safety issues caused by the bacteria have drawn people′s attention increasingly,fast and accurate detection technology becomes an urgent research topic.The biological detection system based on nano-probe technology,graphene transparent electrode(GTE)technology and adenosine triphosphate(ATP)luminescence technology is designed in this paper,it can detect the bacteria of food automatically and rapidly.The results indicate that there is a good correlation between the rapid detecting method and the other conventional approaches.the linear coefficient can be up to 0.972,and the detecting time can be controlled within twenty minutes.The detectable bacteria concentration range reaches 102-106 CFU/mL.Moreover,a series of curves obtained by measurements for different polluted foods show that the system has a satisfactory performance.It is sufficient to meet the requirements of on-site rapid detection.
引文
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[2]Fisher M,Atiya-Nasagi Y,Simon I,et al.A combined immunomagnetic separation and lateral flow method for a sensitive on-site detection of Bacillus anthracis,spores–assessment in water and dairy products[J].Letters in Applied Microbiology,2010,48(4):413-418.
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[4]Novoselov K S,Geim A K,Morozov S V,et al.Electric field effect in atomically thin carbon films[J].science,2004,306(5696):666-669.
[5]Li X,Cai W,An J,et al.Large-area synthesis of high-quality and uniform graphene films on copper foils[J].Science,2009,324(5932):1312-1314.
[6]Hawronskyj J M,Holah J.ATP:A universal hygiene monitor[J].Trends in Food Science&Technology,1997,8(3):79-84.
[7]Zhang Z,Wang C,Zhang L,etal.FastdetectionofE.coliin food using nanoprobe and ATP bioluminescence technology[J].Analytical Methods,2017,9(36):5378-5387.
[8]Li L,Yin D,Xu K,et al.A sandwich immunoassay for brucellosis diagnosis based on immune magnetic beads and quantum dots.[J].Journal of Pharmaceutical&Biomedical Analysis,2017(141):79.
[9]Vila A,Martins V C,Chicharo A,et al.Customized Design of Magnetic Beads for Dynamic Magnetoresistive Cytometry[J].Magnetics IEEE Transactions on,2014,50(11):1-4.
[10]Liu J,Jiang J,An Z,et al.Preparation of a monoclonal antibody based sandwich enzyme-linked immunosorbent assay for Salmonella[C].//IEEE,International Conference on Communication Software and Networks.IEEE,2011:492-496.
[11]Jo G,Choe M,Lee S,et al.The application of graphene as electrodes in electrical and optical devices[J].Nanotechnology,2012,23(11):112001.
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[13]Jung S,Lim K,Kang J W,et al.Electromechanical properties of indium-tin-oxide/poly(3,4-ethylenedioxythiophene):Poly(styrenesulfonate)hybrid electrodes for flexible transparent electrodes[J].Thin Solid Films,2014,550(1):435-443.
[14]Blake P,Brimicombe P D,Nair R R,et al.Graphene-based liquid crystal device[J].Nano letters,2008,8(6):1704-1708.
[15]Li W,Tan C,Lowe M A,et al.Electrochemistry of individual monolayer graphene sheets.[J].Acs Nano,2011,5(3):2264.
[16]Huang X,Zeng Z,Fan Z,et al.Graphene-based electrodes.[J].Advanced Materials,2012,24(45):5979-6004.