6种中草药对多重耐药菌的抗菌活性及逆转耐药性作用的体外研究
|
摘要
目的筛选抗菌活性较强并可逆转细菌耐药性的中草药,为治疗多重耐药菌感染提供新的思路。方法以赤芍、青黛、五倍子、野菊花、鱼腥草、小檗碱等6种临床常用抗菌中草药为研究对象,煎煮法制备提取液后,采用2倍稀释法观察6种中草药对多重耐药铜绿假单胞菌(MDR-PA)与多重耐药鲍曼不动杆菌(MDRAB)的抑菌效果;采用纸片扩散(K-B)法检测中草药对多重耐药菌及原始菌株的最低抑菌浓度,计算两者抑菌圈差值,评估中草药逆转多重耐药菌耐药性的效果。结果 6种中草药对MDR-AB与MDR-PA有不同程度的抑制作用,抑菌作用由强到弱依次为五倍子、鱼腥草、野菊花、赤芍、青黛、小檗碱,五倍子对MDR-AB与MDRPA抑菌效价为512~1 024,抑菌作用最强;五倍子与小檗碱对MDR-AB与MDR-PA的敏感性在逆转前后的抑菌圈直径均值比较,差异有统计学意义(P<0.05),但逆转前后抑菌圈直径均值的差值小于3mm。结论赤芍、青黛、五倍子、野菊花、鱼腥草、小檗碱对MDR-AB与MDR-PA有不同程度抑制作用,五倍子抑菌作用最强;五倍子与小檗碱对MDR-AB与MDR-PA的耐药性有一定程度逆转作用。
Objective To filter the traditional Chinese medicine with strong antibacterial activity and effects of reversing drug resistance of bacteria for providing new ideas for the treatment of multi-drug resistant bacteria infection.Methods Six kinds of antibacterial Chinese traditional medicines commonly used in clinic treatment as Radix Paeoniae Rubra,Indigo Naturalis,Galla Chinensis,Flos Chrysanthemi Indici,Herba Houttuyniae,and Berberine were used in this study.After preparation of the extract using decocting method,bacteriostatic effects of these six kinds of Chinese medicine on multiple resistant bacteria were detected by double dilution method.To evaluate the effects of Chinese medicines on reversing drug resistance of multi-drug resistant bacteria,minimum inhibitory concentrations for multi-drug resistant bacteria and the original strain were detected by K-B method and calculated the difference of the bacterial inhibition rings.Results The six kinds of traditional Chinese medicines had different degrees of inhibitory effects on MDR-AB and MDR-PA,and the bacteriostatic effect sequence were Galla Chinensis,Herba Houttuyniae,Flos Chrysanthemi Indici,Radix Paeoniae Rubra,Indigo Naturalis,and Berberine.The bacteriostatic titer of Galla Chinensis on MDR-AB and MDR-PA was 512-1 024,whose antibacterial activity was the strongest in the six antibacterial Chinese traditional medicines.Galla chinensis and Berberine had significant effects on reversing drug resistance of MDR-AB MDR-PA(P<0.05),but the difference value of inhibition zone diameters before and after reversing were less than 3 mm.Conclusion Radix Paeoniae Rubra,Indigo Naturalis,Galla Chinensis,Flos Chrysanthemi Indici,Herba Houttuyniae,and Berberine could inhibit MDR-AB and MDR-PA in different degrees,in which Galla Chinensis was the most effective antibacterial Chinese Medicine.Ggalla chinensis and Berberine could reverse drug resistance of MDR-AB and MDR-PA to some extent.
Objective To filter the traditional Chinese medicine with strong antibacterial activity and effects of reversing drug resistance of bacteria for providing new ideas for the treatment of multi-drug resistant bacteria infection.Methods Six kinds of antibacterial Chinese traditional medicines commonly used in clinic treatment as Radix Paeoniae Rubra,Indigo Naturalis,Galla Chinensis,Flos Chrysanthemi Indici,Herba Houttuyniae,and Berberine were used in this study.After preparation of the extract using decocting method,bacteriostatic effects of these six kinds of Chinese medicine on multiple resistant bacteria were detected by double dilution method.To evaluate the effects of Chinese medicines on reversing drug resistance of multi-drug resistant bacteria,minimum inhibitory concentrations for multi-drug resistant bacteria and the original strain were detected by K-B method and calculated the difference of the bacterial inhibition rings.Results The six kinds of traditional Chinese medicines had different degrees of inhibitory effects on MDR-AB and MDR-PA,and the bacteriostatic effect sequence were Galla Chinensis,Herba Houttuyniae,Flos Chrysanthemi Indici,Radix Paeoniae Rubra,Indigo Naturalis,and Berberine.The bacteriostatic titer of Galla Chinensis on MDR-AB and MDR-PA was 512-1 024,whose antibacterial activity was the strongest in the six antibacterial Chinese traditional medicines.Galla chinensis and Berberine had significant effects on reversing drug resistance of MDR-AB MDR-PA(P<0.05),but the difference value of inhibition zone diameters before and after reversing were less than 3 mm.Conclusion Radix Paeoniae Rubra,Indigo Naturalis,Galla Chinensis,Flos Chrysanthemi Indici,Herba Houttuyniae,and Berberine could inhibit MDR-AB and MDR-PA in different degrees,in which Galla Chinensis was the most effective antibacterial Chinese Medicine.Ggalla chinensis and Berberine could reverse drug resistance of MDR-AB and MDR-PA to some extent.
引文
[1]段欣冉,姜志辉,杨相海,等.中药抑制鲍曼不动杆菌研究进展[J].中国感染控制杂志,2017,16(6):577-581.
[2]宓伟,曲巍.对抗绿脓杆菌的中药筛选及联合应用的研究[J].滨州医学院学报,2012,35(5):359-361.
[3]何先元,李坤,喻录容,等.53味清热类中药对绿脓杆菌抑菌作用的聚类分析[J].中国实验方剂学杂志,2014,20(20):222-225.
[4]李春辉.MDR、XDR、PDR多重耐药菌暂行标准定义--国际专家建议[J].中国感染控制杂志,2014,13(1):62-64.
[5]李建志,刘文丽,王亚贤.9种中草药抗菌作用实验研究[J].中医药学报,2012,40(1):45-46.
[6]芦亚君,程宁.3种中药方剂逆转大肠杆菌耐药性的实验观察[J].西北药学杂志,2007,22(6):309-311.
[7]张小江,张辉,窦红涛,等.2012年北京协和医院细菌耐药性监测[J].中国感染与化疗杂志,2014,14(2):104-111.
[8]杨海慧,韩立中,刘怡菁,等.亚胺培南联合多黏菌素B对不同表型铜绿假单胞菌体外抗菌活性的研究[J].检验医学,2013,28(1):1-6.
[9]刘立凡,陈佰义.多黏菌素B与亚胺培南联用对泛耐药鲍曼不动杆菌体外抗菌活性的研究[J].中国医疗前沿,2013,8(2):4-5.
[10]金建玲,徐轲,高培基.中西药联合使用在应对细菌抗药性方面的优势[J].中医药学报,2011,39(4):95-98.
[11]ZHANG G B,GONG S Y,YU D H,et al.Propolis and herba epimedii extracts enhance the non-specific immune response and disease resistance of Chinese sucker,myxocyprinus asiaticus[J].Fish Shellfish Immunol,2009,26(3):467-472.
[12]贾云鹏,程宁.中药对大肠埃希菌抗菌药物耐药性逆转作用的实验研究[J].陕西中医,2009,30(3):366-368.
[13]陈美玲,王剑,周红霞,等.中药饮片对多重抗菌药物耐药细菌的抑菌作用[J].中医药学报,2012,40(2):10-13.
[14]蔡燕,邢艳,谢文光.中药抗菌作用研究进展[J].中国医药导报,2011,8(6):9-10.
[15]KAPP E,WHITELEY C.Protein ligand interactions:isoquinoline alkaloids as inhibitors for lactate and malate dehydrogenase[J].J Enzyme Inhib,1991,4(3):233-243.
[16]袁莉莉,丁百兴,沈震,等.碳青霉烯类抗菌药物耐药铜绿假单胞菌感染的临床研究[J].中国感染与化疗杂志,2017,17(2):121-126.
[17]张岩岩,朱婉,张静萍,等.多重耐药鲍曼不动杆菌血流感染危险因素分析[J].中国感染与化疗杂志,2017,17(2):135-139.
[18]GEORGE T,FRANK R.Mutidrug pump inhibitors uncover remarkable activity of plant antimicrobials[J].Antimicrob Agent Chemother,2002,46(10):3133.
[19]崔煦然,赵京霞,郭玉红,等.细菌耐药背景下中药抗菌作用研究进展[J].世界中医药,2016,11(10):1940-1944.
[20]李玉洋.中西医结合治疗慢性盆腔炎的疗效观察[J].中国医药指南,2017,15(3):174.
[21]潘杰,苗霞,刘启波,等.12种中药配方颗粒体外逆转临床常见细菌耐药性分析[J].检验医学,2016,36(6):503-508.
[22]王洁,朱俊豪,张斌,等.能逆转细菌耐药性的中药的筛选[J].中国药学杂志,2014,49(21):1892-1896.
[23]李娟,李晓东,杨丽霞,等.单味中药体外抑菌活性的研究进展[J].中国实验方剂学杂志,2011,17(11):283-286.
[2]宓伟,曲巍.对抗绿脓杆菌的中药筛选及联合应用的研究[J].滨州医学院学报,2012,35(5):359-361.
[3]何先元,李坤,喻录容,等.53味清热类中药对绿脓杆菌抑菌作用的聚类分析[J].中国实验方剂学杂志,2014,20(20):222-225.
[4]李春辉.MDR、XDR、PDR多重耐药菌暂行标准定义--国际专家建议[J].中国感染控制杂志,2014,13(1):62-64.
[5]李建志,刘文丽,王亚贤.9种中草药抗菌作用实验研究[J].中医药学报,2012,40(1):45-46.
[6]芦亚君,程宁.3种中药方剂逆转大肠杆菌耐药性的实验观察[J].西北药学杂志,2007,22(6):309-311.
[7]张小江,张辉,窦红涛,等.2012年北京协和医院细菌耐药性监测[J].中国感染与化疗杂志,2014,14(2):104-111.
[8]杨海慧,韩立中,刘怡菁,等.亚胺培南联合多黏菌素B对不同表型铜绿假单胞菌体外抗菌活性的研究[J].检验医学,2013,28(1):1-6.
[9]刘立凡,陈佰义.多黏菌素B与亚胺培南联用对泛耐药鲍曼不动杆菌体外抗菌活性的研究[J].中国医疗前沿,2013,8(2):4-5.
[10]金建玲,徐轲,高培基.中西药联合使用在应对细菌抗药性方面的优势[J].中医药学报,2011,39(4):95-98.
[11]ZHANG G B,GONG S Y,YU D H,et al.Propolis and herba epimedii extracts enhance the non-specific immune response and disease resistance of Chinese sucker,myxocyprinus asiaticus[J].Fish Shellfish Immunol,2009,26(3):467-472.
[12]贾云鹏,程宁.中药对大肠埃希菌抗菌药物耐药性逆转作用的实验研究[J].陕西中医,2009,30(3):366-368.
[13]陈美玲,王剑,周红霞,等.中药饮片对多重抗菌药物耐药细菌的抑菌作用[J].中医药学报,2012,40(2):10-13.
[14]蔡燕,邢艳,谢文光.中药抗菌作用研究进展[J].中国医药导报,2011,8(6):9-10.
[15]KAPP E,WHITELEY C.Protein ligand interactions:isoquinoline alkaloids as inhibitors for lactate and malate dehydrogenase[J].J Enzyme Inhib,1991,4(3):233-243.
[16]袁莉莉,丁百兴,沈震,等.碳青霉烯类抗菌药物耐药铜绿假单胞菌感染的临床研究[J].中国感染与化疗杂志,2017,17(2):121-126.
[17]张岩岩,朱婉,张静萍,等.多重耐药鲍曼不动杆菌血流感染危险因素分析[J].中国感染与化疗杂志,2017,17(2):135-139.
[18]GEORGE T,FRANK R.Mutidrug pump inhibitors uncover remarkable activity of plant antimicrobials[J].Antimicrob Agent Chemother,2002,46(10):3133.
[19]崔煦然,赵京霞,郭玉红,等.细菌耐药背景下中药抗菌作用研究进展[J].世界中医药,2016,11(10):1940-1944.
[20]李玉洋.中西医结合治疗慢性盆腔炎的疗效观察[J].中国医药指南,2017,15(3):174.
[21]潘杰,苗霞,刘启波,等.12种中药配方颗粒体外逆转临床常见细菌耐药性分析[J].检验医学,2016,36(6):503-508.
[22]王洁,朱俊豪,张斌,等.能逆转细菌耐药性的中药的筛选[J].中国药学杂志,2014,49(21):1892-1896.
[23]李娟,李晓东,杨丽霞,等.单味中药体外抑菌活性的研究进展[J].中国实验方剂学杂志,2011,17(11):283-286.