PPAR-γ和P38MAPK蛋白在肝细胞癌中的表达及临床意义
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摘要
目的探讨人肝细胞癌组织中过氧化物酶体增殖物激活受体γ(PPAR-γ)和P38MAPK蛋白的表达及其临床意义。方法应用免疫组织化学方法检测57例肝细胞癌及20例正常肝组织中PPAR-γ和P38MAPK蛋白的表达,分析其与肝细胞癌临床病理特征及预后的关系。结果肝细胞癌组织中PPAR-γ蛋白的阳性表达率为45.6%,显著高于正常肝组织(15.0%,P<0.05);P38MAPK蛋白的高表达率为64.9%,显著高于正常肝组织(14.3%,P<0.05)。PPAR-γ蛋白的表达与肿瘤直径、门静脉癌栓及TNM分期有关(P<0.05);P38MAPK蛋白的表达与肿瘤直径、门静脉癌栓、侵及周围脏器或淋巴结转移及TNM分期无关。PPAR-γ与P38MAPK蛋白表达呈正相关(r=0.378,P=0.004)。P38MAPK蛋白和PPAR-γ蛋白的表达与患者术后生存率无关。结论肝细胞癌组织中PPAR-γ蛋白的表达水平与肝细胞癌的恶性生物学行为密切相关,PPAR-γ与P38MAPK蛋白在肝细胞癌的发生、发展过程中发挥作用。
引文
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8 Keshet Y,Seger R.The MAP kinase signaling cascades:a system of hundreds of components regulates a diverse array of physiological functions〔J〕.Methods Mol Biol,2010;661:3-38.
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10 徐正府,任雪霞,黄介飞,等.MMP-13及P38MAPK在肝细胞癌侵袭和转移中的作用〔J〕.苏州大学学报(医学版),2008;28(6):966-9.
11 Kim EK,Choi EJ.Pathological roles of MAPK signaling pathways in human diseases〔J〕.Biochim Biophys Acta,2010;1802(4):396-405.
12 刘瑞,高维娟.丝裂原活化蛋白激酶信号通路及其在细胞凋亡中的作用〔J〕.中国老年学杂志,2012;32(18):4089-92.
13 刘加军,徐妍,刘文迭,等.环格列酮通过PPARr及P38MAPK信号途径诱导白血病HL-60细胞凋亡〔J〕.中华医学杂志,2010;90(32):2270-4.
2 del Barco Barrantes I,Nebreda AR.Roles of p38 MAPKs in invasion and metastasis〔J〕.Biochem Soc Trans,2012;40(1):79-84.
3 Fujita M,Yagami T,Fujio M,et al.Cytotoxicity of troglitazone through PPARγ-independent pathway and p38 MAPK pathway in renal cell carcinoma〔J〕.Cancer Lett,2011;312(2):219-27.
4 Yao L,Liu F,Sun L,et al.Upregulation of PPARgamma in tissue with gastric carcinoma〔J〕.Hybridoma(Larchmt),2010;29(4):341-3.
5 Lee SD.Immunohistochemical analysis of nuclear factor,p38,and cyclin D1 proteins in premalignant lesions and carcinomas of the colorectal mucosa〔J〕.Korean J Gastroenterol,2008;52(6):359-67.
6 Wang W,Wang R,Zhang Z,et al.Enhanced PPAR-gamma expression may correlate with the development of Barrett's esophagus and esophageal adenocarcinoma〔J〕.Oncol Res,2011;19(3-4):141-7.
7 Giaginis C,Katsamangou E,Tsourouflis G,et al.Peroxisome proliferatoractivated receptor-gamma and retinoid X receptor-alpha expression in pancreatic ductal adenocarcinoma:association with clinicopathological parameters,tumor proliferative capacity,and patients'survival〔J〕.Med Sci Monit,2009;15(5):148-56.
8 Keshet Y,Seger R.The MAP kinase signaling cascades:a system of hundreds of components regulates a diverse array of physiological functions〔J〕.Methods Mol Biol,2010;661:3-38.
9 De la Cruz-Morcillo MA,Valero ML,Callejas-Valera JL,et al.P38MAPK is a major determinant of the balance between apoptosis and autophagy triggered by 5-fluorouracil:implication in resistance〔J〕.Oncogene,2012;31(9):1073-85.
10 徐正府,任雪霞,黄介飞,等.MMP-13及P38MAPK在肝细胞癌侵袭和转移中的作用〔J〕.苏州大学学报(医学版),2008;28(6):966-9.
11 Kim EK,Choi EJ.Pathological roles of MAPK signaling pathways in human diseases〔J〕.Biochim Biophys Acta,2010;1802(4):396-405.
12 刘瑞,高维娟.丝裂原活化蛋白激酶信号通路及其在细胞凋亡中的作用〔J〕.中国老年学杂志,2012;32(18):4089-92.
13 刘加军,徐妍,刘文迭,等.环格列酮通过PPARr及P38MAPK信号途径诱导白血病HL-60细胞凋亡〔J〕.中华医学杂志,2010;90(32):2270-4.