生物淋滤中硫粉对污泥EPS组分和脱水性的影响
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摘要
为探讨生物淋滤过程中污泥的脱水性能及其与胞外聚合物(EPS)中蛋白质(PN)、多糖(PS)的关系,以硫粉(S~0)作为底物,设计了不同S~0投量(0,2,4,6,8g/L)+10%接种物的实验组进行10d淋滤实验.结果表明:溶解态PN、PS含量和污泥比阻(SRF)在淋滤0～2d降低,之后呈上升趋势,结合态PN、PS含量在淋滤0～8d呈下降趋势,之后略微上升,且S~0投加量越大,上述变化趋势越明显.统计结果发现,SRF与溶解态PN、PS有显著的正相关性(R=0.861、0.875,P<0.01).说明不同S0投加量导致溶解态PN、PS含量变化从而影响了污泥脱水性能,其中溶解态PS的变化对污泥脱水性能作用更大.S~0投量为8g/L、淋滤时间为2d时,污泥脱水效果最佳, S0投量为2g/L、淋滤时间6d效果次之,SRF分别比原污泥降低58%和54%,又由于后者重金属去除率高于前者,推荐S0 2g/L,温度30℃,淋滤时间6d,p H值降至2.66为最适工艺条件.
To investigate the dewaterability of sludge and its relationship with protein(PN), polysaccharide(PS) of extracellular polymers(EPS) in sludge bioleaching, S~0 was added as substrate with different dosage of S~0(0 g/L, 2 g/L, 4 g/L, 6 g/L, 8 g/L) +10% inoculum for 10 days. Results showed that dissolved PS, PN and sludge specific resistance(SRF) reduced at 0～2 days, but increased later, while the bound PN and PS decreased during 0～8 d of bioleaching, and slightly increased later. Moreover, the above trend increased with the increasing loadings of S~0. Statistical results showed that the SRF and dissolved PN, PS had a significantly positive correlation(R=0.861, 0.875, P<0.01), suggesting that the content of dissolved PN and PS were influenced by S~0, thus affecting the dewatering performance of sludge, in which, the dissolved PS had more effect on the dewatering of sludge. The best bioleaching process for sludge dewatering performance was 8 g/L of S~0 for 2 days, followed by 2 g/L of S~0 for 6 days. with the SRF 58% and 54% lower than that of the original sludge, respectively. The heavy metal removal rate in the latter case was higher, thus the latter with 30℃, p H dropped to 2.66 was recommended as the optimization conditions.
To investigate the dewaterability of sludge and its relationship with protein(PN), polysaccharide(PS) of extracellular polymers(EPS) in sludge bioleaching, S~0 was added as substrate with different dosage of S~0(0 g/L, 2 g/L, 4 g/L, 6 g/L, 8 g/L) +10% inoculum for 10 days. Results showed that dissolved PS, PN and sludge specific resistance(SRF) reduced at 0～2 days, but increased later, while the bound PN and PS decreased during 0～8 d of bioleaching, and slightly increased later. Moreover, the above trend increased with the increasing loadings of S~0. Statistical results showed that the SRF and dissolved PN, PS had a significantly positive correlation(R=0.861, 0.875, P<0.01), suggesting that the content of dissolved PN and PS were influenced by S~0, thus affecting the dewatering performance of sludge, in which, the dissolved PS had more effect on the dewatering of sludge. The best bioleaching process for sludge dewatering performance was 8 g/L of S~0 for 2 days, followed by 2 g/L of S~0 for 6 days. with the SRF 58% and 54% lower than that of the original sludge, respectively. The heavy metal removal rate in the latter case was higher, thus the latter with 30℃, p H dropped to 2.66 was recommended as the optimization conditions.
引文
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[14]Wang X M,Wang X,Yang M H,et al.Sludge Conditioning Performance of Polyaluminum,Polyferric,and Titanium Xerogel Coagulants[J].Environmental Science,2018:2274-2282.
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[17]Wingender J,Neu T R,Flemming H C.Microbial Extracellular Polymeric Substances,Characterization,Structure and Function[M].Springer Berlin Heidelberg,1999,88(1):45-53.
[18]王红武,李晓岩,赵庆祥.活性污泥的表面特性与其沉降脱水性能的关系[J].清华大学学报(自然科学版),2004,44(6):766-769.Wang H W,Li X Y,Zhao Q X.Surface properties of activated sludge and their effects on settlleability and dewaterability[J].J Tsinghua Univ(Sci&Tech),2004,44(6):766-769.
[19]华玉妹,陈英旭,张少辉.污泥生物沥滤中硫细菌变化和胞外多聚物作用的研究[J].中国环境科学,2011,31(5):795-802.Hua Y M,Chen Y X,Zhang S H.Variation in sulfur bacteria and extracellular polymeric substances during sewage sludge bioleaching.[J].China Environmental Science,2011,31(5):795-802.
[20]Lowry O H,Rosebrough N J,Farr A L,et al.Protein measurement with the folin phenol reagent[J].Journal of Biological Chemistry,1951,193(1):265-275.
[21]Gaudy A F.Colorimetric determination of protein and carbohydrate[J].Industrial Water Wastes,1962,7(1):17-22.
[22]吴志高.胞外聚合物(EPS)对污泥沉降性能的影响及其在生物除磷中的作用研究[D].重庆:重庆大学,2006.Wu Z G.Study on the Effect of Extracelluar Polymer Substances on the Settleability of the Sludge and its Role in Biological Removal of Phosphorus[D].Chongqing:Chongqing University,2006.
[23]D’Abzac P,Bordas F,Van H E,et al.Effects of extraction procedures on metal binding properties of extracellular polymeric substances(EPS)from anaerobic granular sludges[J].Colloids&Surfaces BBiointerfaces,2010,80(2):161-168.
[24]Chen Y G,Yang H Z,Gu G W.Effect of acid and surfactant treatment on activated sludge dewatering and settling[J].Water Research,2001,35(11):2615-2620.
[25]周立祥.污泥生物沥浸处理技术及其工程应用[J].南京农业大学学报,2012,35(5):154-166.Zhou L X.Bioleaching role in improving sludge in-deep dewatering and removal of sludge-borne metals and its engineering application[J].Journal of Nanjing Agricultural University,2012,35(5):154-166.
[26]黄晓婷,袁海平,周熠鸣,等.基质投加量对生物调理改善污泥脱水性能的影响[J].环境科学学报,2017,37(6):2137-2142.Huang X T,Yuan H P,Zhou Y M,et al.Effect of agent dosage on sludge dewaterability by biological conditioning[J].Acta Scientiae Circumstantiae,2017,37(6):2137-2142.
[27]王子超,高孟春,魏俊峰,等.盐度变化对厌氧污泥胞外聚合物的影响[J].环境科学学报,2016,36(9):3273-3281.Wang Z C,Gao M C,Wei J F,et al.Effect of salinity on extracellular polymeric substances of anaerobic sludge[J].Acta Scientiae Circumstantiae,2016,36(9):3273-3281.
[28]Guan Y,Huang B,Qian C,et al.Improved PVDF membrane performance by doping extracellular polymeric substances of activated sludge.[J].Water Research,2017,113:89-96.
[2]Gu T Y,Rasteger S O,Mousavi S M,et al.Advances in bioleaching for recovery of metals and bioremediation of fuel ash and sewage sludge.[J].Bioresource Technology,2018,261:428-440.
[3]Mehrotra A,Sreekrishnan T R.Heavy metal bioleaching and sludge stabilization in a single-stage reactor using indigenous acidophilic heterotrophs[J].Environmental Technology,2017,38(21):1-16.
[4]Deng X,Chai L,Yang Z,et al.Bioleaching mechanism of heavy metals in the mixture of contaminated soil and slag by using indigenous Penicillium chrysogenum strain F1.[J].Journal of Hazardous Materials,2013,248-249(15):107-114.
[5]甘莉,刘贺琴,王清萍,等.氧化亚铁硫杆菌生物浸出污泥中的重金属离子[J].中国环境科学,2014,34(10):2617-2623.Gan L,Liu H Q,Wang Q P,et al.Bioleaching of heavy metals in sewage sludge using Acidithiobacillus ferrooxidans.[J].China Environmental Science,2014,34(10):2617-2623.
[6]向少云,田永静,夏晶,等.生物沥滤法对剩余污泥中重金属及养分含量的影响[J].中国给水排水,2018,34(1):20-25.Xiang S Y,Tian Y J,Xia J,et al.Influence of bioleaching on heavy metals and nutrient content of municipal sewage sludge[J].China Water&Wastewater,2018,34(1):20-25.
[7]向少云.生物沥滤法对城市污泥中重金属去除及其对污泥脱水性能影响的研究[D].苏州:苏州科技大学,2017.Xiang S Y.The influence of bioleaching on heavy metals and Dehydration Performance of municipal sewage sludge[D].Suzhou:Suzhou University of Science and Technology,2017.
[8]石超宏,朱能武,吴平霄,等.生物沥浸去除污泥重金属及改善脱水性能研究[J].中国环境科学,2013,33(3):474-479.Shi C H,Zhu N W,Wu P X,et al.Heavy metals removal from sewage sludge and dewaterability improvement by bioleaching.[J].China Environmental Science,2013,33(3):474-479.
[9]Liu H,Yang S,Shi J,et al.Towards understanding the dewatering mechanism of sewage sludge improved by bioleaching processing[J].Separation&Purification Technology,2016,165:53-59.
[10]Huo M,Zheng G,Zhou L.Enhancement of the dewaterability of sludge during bioleaching mainly controlled by microbial quantity change and the decrease of slime extracellular polymeric substances content[J].Bioresour Technol,2014,168(3):190-197.
[11]Dai Q,Ma L,Ren N,et al.Investigation on extracellular polymeric substances,sludge flocs morphology,bound water release and dewatering performance of sewage sludge under pretreatment with modified phosphogypsum[J].Water Research,2018,142:337.
[12]周俊,周立祥,黄焕忠,等.污泥胞外聚合物的提取方法及其对污泥脱水性能的影响[J].环境科学,2013,34(7):2752-2757.Zhou J,Zhou L X,Huang H Z,et al.Optimization of Extracellular Polymeric Substance Extraction Method and its Role in the Dewaterability of Sludge[J].Environmental Science,2013,34(7):2752-2757.
[13]Dai Y,Huang S,Liang J,et al.Role of organic compounds from different EPS fractions and their effect on sludge dewaterability by combining anaerobically mesophilic digestion pre-treatment and Fenton’s reagent/lime[J].Chemical Engineering Journal,2017,321:123-138.
[14]Wang X M,Wang X,Yang M H,et al.Sludge Conditioning Performance of Polyaluminum,Polyferric,and Titanium Xerogel Coagulants[J].Environmental Science,2018:2274-2282.
[15]Xiao K,Chen Y,Jiang X,et al.Characterization of key organic compounds affecting sludge dewaterability during ultrasonication and acidification treatments[J].Water Research,2016,105:470-478.
[16]刘昌庚.基于生物淋滤的城市污泥调理技术[D].湖南:湖南大学,2010.Liu C G.Conditioning Technologies on the Basis of Sewage Sludge Bioleaching[D].Hunan:Hunan University,2010.
[17]Wingender J,Neu T R,Flemming H C.Microbial Extracellular Polymeric Substances,Characterization,Structure and Function[M].Springer Berlin Heidelberg,1999,88(1):45-53.
[18]王红武,李晓岩,赵庆祥.活性污泥的表面特性与其沉降脱水性能的关系[J].清华大学学报(自然科学版),2004,44(6):766-769.Wang H W,Li X Y,Zhao Q X.Surface properties of activated sludge and their effects on settlleability and dewaterability[J].J Tsinghua Univ(Sci&Tech),2004,44(6):766-769.
[19]华玉妹,陈英旭,张少辉.污泥生物沥滤中硫细菌变化和胞外多聚物作用的研究[J].中国环境科学,2011,31(5):795-802.Hua Y M,Chen Y X,Zhang S H.Variation in sulfur bacteria and extracellular polymeric substances during sewage sludge bioleaching.[J].China Environmental Science,2011,31(5):795-802.
[20]Lowry O H,Rosebrough N J,Farr A L,et al.Protein measurement with the folin phenol reagent[J].Journal of Biological Chemistry,1951,193(1):265-275.
[21]Gaudy A F.Colorimetric determination of protein and carbohydrate[J].Industrial Water Wastes,1962,7(1):17-22.
[22]吴志高.胞外聚合物(EPS)对污泥沉降性能的影响及其在生物除磷中的作用研究[D].重庆:重庆大学,2006.Wu Z G.Study on the Effect of Extracelluar Polymer Substances on the Settleability of the Sludge and its Role in Biological Removal of Phosphorus[D].Chongqing:Chongqing University,2006.
[23]D’Abzac P,Bordas F,Van H E,et al.Effects of extraction procedures on metal binding properties of extracellular polymeric substances(EPS)from anaerobic granular sludges[J].Colloids&Surfaces BBiointerfaces,2010,80(2):161-168.
[24]Chen Y G,Yang H Z,Gu G W.Effect of acid and surfactant treatment on activated sludge dewatering and settling[J].Water Research,2001,35(11):2615-2620.
[25]周立祥.污泥生物沥浸处理技术及其工程应用[J].南京农业大学学报,2012,35(5):154-166.Zhou L X.Bioleaching role in improving sludge in-deep dewatering and removal of sludge-borne metals and its engineering application[J].Journal of Nanjing Agricultural University,2012,35(5):154-166.
[26]黄晓婷,袁海平,周熠鸣,等.基质投加量对生物调理改善污泥脱水性能的影响[J].环境科学学报,2017,37(6):2137-2142.Huang X T,Yuan H P,Zhou Y M,et al.Effect of agent dosage on sludge dewaterability by biological conditioning[J].Acta Scientiae Circumstantiae,2017,37(6):2137-2142.
[27]王子超,高孟春,魏俊峰,等.盐度变化对厌氧污泥胞外聚合物的影响[J].环境科学学报,2016,36(9):3273-3281.Wang Z C,Gao M C,Wei J F,et al.Effect of salinity on extracellular polymeric substances of anaerobic sludge[J].Acta Scientiae Circumstantiae,2016,36(9):3273-3281.
[28]Guan Y,Huang B,Qian C,et al.Improved PVDF membrane performance by doping extracellular polymeric substances of activated sludge.[J].Water Research,2017,113:89-96.