谷子花药颜色基因Siac1的精细定位
|
摘要
为从分子水平上揭示谷子花药颜色性状的遗传基础,以‘E1005’为母本,‘品资39号’为父本构建F2分离群体,利用集团分离分析法(BSA)结合隐性群体分析法(RCA),对谷子花药颜色基因Siac1进行精细定位。遗传分析表明,F2植株黄色花药与白色花药分离比例符合3:1的孟德尔分离规律,表明白色花药性状由一对隐性核基因控制。利用SSR和SV标记将Siac1初定位于第VI染色体上标记GSA07025和GSA07037之间约282 kb的区间内。进一步利用根据亲本重测序结果新开发的InDel标记,最终将Siac1精细定位于标记MRI579和MRI583之间约94.7 kb区段内。生物信息学分析表明,该区间共有10个开放阅读框,初步推测Seita.6G227100、Seita.6G227200、Seita.6G227300和Seita.6G227900为花药颜色性状的候选基因。本研究为克隆Siac1基因、解析花药颜色发育机制奠定了一定基础。
To reveal the genetic basis of anther color in foxtail millet at molecular level, an F2 segregating population derived from a crossing between‘E1005'(female parent) and‘Pinzi 39'(male parent) was used as mapping population. With bulked segregation analysis(BSA) and recessive-class analysis(RCA), the Siac1 related to anther color was finely mapped. Genetic analysis showed that the segregation ratio of yellow anther plants to white anther plants was consistent with the Mendelian segregation of 3:1 in the F2 generation, which suggested that the white anther color trait was controlled by a pair of recessive nuclear genes. The Siac1 was preliminary mapped onto an interval of about 282 kb between markers GSA07025 and GSA07037 on chromosome VI by SSR markers and SV markers. Furthermore, fine mapping of Siac1 was conducted using newly developed InDel markers based on the sequence differences between the two parents, and the results indicated that the Siac1 was narrowed down onto a genomic region of around 94.7 kb between markers MRI579 and MRI583. Bioinformatics analysis showed that there were 10 Open Reading Frames(ORFs), and Seita.6 G227100, Seita.6 G227200, Seita.6 G227300 and Seita.6 G227900 could be speculated to be four candidates for Siac1. This research lays a foundation for the cloning of Siac1 and the analysis of developmental mechanism of anther color.
To reveal the genetic basis of anther color in foxtail millet at molecular level, an F2 segregating population derived from a crossing between‘E1005'(female parent) and‘Pinzi 39'(male parent) was used as mapping population. With bulked segregation analysis(BSA) and recessive-class analysis(RCA), the Siac1 related to anther color was finely mapped. Genetic analysis showed that the segregation ratio of yellow anther plants to white anther plants was consistent with the Mendelian segregation of 3:1 in the F2 generation, which suggested that the white anther color trait was controlled by a pair of recessive nuclear genes. The Siac1 was preliminary mapped onto an interval of about 282 kb between markers GSA07025 and GSA07037 on chromosome VI by SSR markers and SV markers. Furthermore, fine mapping of Siac1 was conducted using newly developed InDel markers based on the sequence differences between the two parents, and the results indicated that the Siac1 was narrowed down onto a genomic region of around 94.7 kb between markers MRI579 and MRI583. Bioinformatics analysis showed that there were 10 Open Reading Frames(ORFs), and Seita.6 G227100, Seita.6 G227200, Seita.6 G227300 and Seita.6 G227900 could be speculated to be four candidates for Siac1. This research lays a foundation for the cloning of Siac1 and the analysis of developmental mechanism of anther color.
引文
[1]刁现民.中国谷子产业与产业技术体系[M].北京:中国农业科学技术出版社,2010:20-27.
[2]柴岩,万福世.中国谷子产业发展报告[M].北京:中国农业出版社,2007:32-43.
[3] La?kova L I, Arbuzova V S, Efremova T T, et al. Genetic analysis of anthocyanin of the anthers and culm pigmentation in common wheat[J]. Genetika,2005,41(10):1428-1433.
[4] Gould K S. Nature's Swiss Army Knife:The diverse protective roles of anthocyanins in leaves[J]. J Biomed Biotechnol., 2004,2004(5):314-320.
[5]袁文娟,覃鸿妮,王旭,等.玉米雄穗颜色的QTL分析[J].植物遗传资源学报,2013,14(2):289-297.
[6] Holton T A, Cornish E C. Genetics and biochemistry of anthocyanin biosynthesis[J].Plant Cell,1995,7:1071-1083.
[7]曹珂,王思倩,朱更瑞,等.桃花粉育性与花药颜色的关系及其SSR分子标记[J].植物遗传资源学报,2010,11(6):817-822.
[8] Blanco A, Bellomo M P, Cenci A, et al. A genetic linkage map of durum wheat[J]. Theoretical&Applied Genetics,1998,97(5-6):721-728.
[9] Khlestkina E K, Pshenichnikova T A, R?der M S, et al. Clustering anthocyanin pigmentation genes in wheat group 7 chromosomes[J].Cereal Research Communications,2009,37(3):391-398.
[10] McIntosh R A, Yamazaki Y, Dubcovsky J, et al. Catalogue of gene symbols for wheat.2003.
[11]李楠楠,张倩,邹少奎,等.小麦紫色花药和紫色叶鞘性状的基因定位[J].农业科技通讯,2016(11):66-68.
[12]耿小红,武艳芍,余马.人工合成六倍体小麦SHW-L1紫色花药的遗传定位研究[J].湖北农业科学,2016,55(22):5748-5750.
[13] Ni X, Xia Q, Zhang H, et al. Updated foxtail millet genome assembly and gene mapping of nine key agronomic traits by resequencing a RIL population[J]. Gigascience,2017,6(2):1-8.
[14] Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis:a rapid method to detect markers in specific genomic regions by using segregating populations[J]. Proceedings of the National Academy of Sciences of the United States of America,1991,88(21):9828-9832.
[15] Zhang Q, Shen B Z, Dai X K, et al. Using bulked extremes and recessive class to map genes for photoperiod-sensitive genic male sterility in rice[J]. Proc Natl Acad Sci U S A,1994,91(18):8675-8679.
[16] Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA[J]. Nucleic Acids Research,1980,8(19):4321-4325.
[17] Marklund S, Chaudhary R, Marklund L, et al. Extensive mtDNA diversity in horses revealed by PCR-SSCP analysis[J]. Animal Genetics,1995,26(3):193-196.
[18] Jia X, Zhang Z, Liu Y, et al. Development and genetic mapping of SSR markers in foxtail millet[Setaria italica(L.)P. Beauv.][J].Theoretical&Applied Genetics,2009,118(4):821-829.
[19] Jia X P, Shi Y S, Song Y C, et al. Development of EST-SSR in foxtail millet(Setaria italica)[J]. Genetic Resources&Crop Evolution,2007,54(2):233-236.
[20] Fang X, Dong K, Wang X, et al. A high density genetic map and QTL for agronomic and yield traits in Foxtail millet[Setaria italica(L.)P. Beauv.][J]. Bmc Genomics,2016,17(1):336.
[21] Zhang G, Liu X, Quan Z, et al. Genome sequence of foxtail millet(Setaria italica)provides insights into grass evolution and biofuel potential[J]. Nature Biotechnology, 2012,30(6):549-554.
[22] Kosambi D D. The Estimation of map distances from recombination values[J]. Annals of Human Genetics,2012,12(1):172-175.
[23]刘仁虎,孟金陵. MapDraw,在Excel中绘制遗传连锁图的宏[J].遗传,2003,25(3):317-321.
[24] Liu B, Wang Y, Zhai W, et al. Development of InDel markers for Brassica rapa based on whole-genome re-sequencing[J]. Theoretical&Applied Genetics, 2013,126(1):231-239.
[25] Jander G, Norris S R, Rounsley S D, et al. Arabidopsis map-based cloning in the post-genome era[J]. Plant Physiology,2002,129(2):440-50.
[26]刘玉林.大豆遗传图谱构建及重要农艺性状QTL定位[D].北京:中国农业科学院,2011.
[27]田希辉,于拴仓,苏同兵,等.一个新的白菜苗期TuMV-C4抗性主效QTL定位及连锁分子标记开发[J].华北农学报,2014,29(6):1-5.
[28]王岩,付新民,高冠军,等.分子标记辅助选择改良优质水稻恢复系明恢63的稻米品质[J].分子植物育种,2009,7(4):661-665.
[29]闻可心,刘雪梅.AP2功能基因在植物花发育中的重要作用[J].生物技术通报,2010(2):1-7.
[30]赵奇,王台,魏小弟.AP2基因在高等植物花器官发育中的作用概述[J].热带农业科学,2005,25(3):50-56.
[31]张妍,刘瀛,孙丰宾,等.白桦APETALA2(AP2)转录因子基因的分离及其表达[J].林业科学研究,2012,25(2):254-260.
[32] Jofuku K D, den Boer B G, Van M M, et al. Control of Arabidopsis flower and seed development by the homeotic gene APETALA2[J].Plant Cell,1994, 6(9):1211-1225.
[33] PréM, Atallah M, Champion A, et al. The AP2/ERF domain transcription factor ORA59 integrates jasmonic acid and ethylene signals in plant defense[J]. Plant Physiology,2008,147(3):1347-1357.
[34]秦捷,王武,左开井,等. AP2基因家族的起源和棉花AP2转录因子在抗病中的作用[J].棉花学报,2005,17(6):366-370.
[35]李雅韵,廖文彬,王斌,等.干旱胁迫下木薯AP2转录因子的荧光定量PCR分析[J].广东农业科学,2014,41(10):126-131.
[36]吴慧敏.水稻AP2/EREBP转录因子基因OsASIE1抗逆功能分析[D].北京:中国农业科学院,2011.
[37]陈旭君,郭泽建.增加表达AP2/EREBP类转录因子OPBP1基因提高烟草的抗逆能力[A].中国植物病理学会2004年学术年会论文集[C].2004.
[38] Manzanares C, Barth S, Thorogood D, et al. A gene encoding a DUF247 domain protein co-segregates with the S selfincompatibility locus in perennial ryegrass[J]. Molecular Biology&Evolution,2016,33(4):870-884.
[39]张浩,汤沙,李迎涛,等.谷子窄颖花突变体sins1的表型分析和突变基因定位[J].植物遗传资源学报,2017,18(3):538-545.
[40] Fan X, Tang S, Zhi H, et al. Identification and fine mapping of SiDWARF3(D3), a pleiotropic locus controlling environmentindependent dwarfism in foxtail millet[J]. Crop Science,2017,57(5).
[41] Li W, Tang S, Zhang S, et al. Gene mapping and functional analysis of the novel leaf color gene SiYGL1 in foxtail millet[Setaria italica(L.)P. Beauv][J]. Physiologia Plantarum,2015,157(1):24-37.
[2]柴岩,万福世.中国谷子产业发展报告[M].北京:中国农业出版社,2007:32-43.
[3] La?kova L I, Arbuzova V S, Efremova T T, et al. Genetic analysis of anthocyanin of the anthers and culm pigmentation in common wheat[J]. Genetika,2005,41(10):1428-1433.
[4] Gould K S. Nature's Swiss Army Knife:The diverse protective roles of anthocyanins in leaves[J]. J Biomed Biotechnol., 2004,2004(5):314-320.
[5]袁文娟,覃鸿妮,王旭,等.玉米雄穗颜色的QTL分析[J].植物遗传资源学报,2013,14(2):289-297.
[6] Holton T A, Cornish E C. Genetics and biochemistry of anthocyanin biosynthesis[J].Plant Cell,1995,7:1071-1083.
[7]曹珂,王思倩,朱更瑞,等.桃花粉育性与花药颜色的关系及其SSR分子标记[J].植物遗传资源学报,2010,11(6):817-822.
[8] Blanco A, Bellomo M P, Cenci A, et al. A genetic linkage map of durum wheat[J]. Theoretical&Applied Genetics,1998,97(5-6):721-728.
[9] Khlestkina E K, Pshenichnikova T A, R?der M S, et al. Clustering anthocyanin pigmentation genes in wheat group 7 chromosomes[J].Cereal Research Communications,2009,37(3):391-398.
[10] McIntosh R A, Yamazaki Y, Dubcovsky J, et al. Catalogue of gene symbols for wheat.2003.
[11]李楠楠,张倩,邹少奎,等.小麦紫色花药和紫色叶鞘性状的基因定位[J].农业科技通讯,2016(11):66-68.
[12]耿小红,武艳芍,余马.人工合成六倍体小麦SHW-L1紫色花药的遗传定位研究[J].湖北农业科学,2016,55(22):5748-5750.
[13] Ni X, Xia Q, Zhang H, et al. Updated foxtail millet genome assembly and gene mapping of nine key agronomic traits by resequencing a RIL population[J]. Gigascience,2017,6(2):1-8.
[14] Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis:a rapid method to detect markers in specific genomic regions by using segregating populations[J]. Proceedings of the National Academy of Sciences of the United States of America,1991,88(21):9828-9832.
[15] Zhang Q, Shen B Z, Dai X K, et al. Using bulked extremes and recessive class to map genes for photoperiod-sensitive genic male sterility in rice[J]. Proc Natl Acad Sci U S A,1994,91(18):8675-8679.
[16] Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA[J]. Nucleic Acids Research,1980,8(19):4321-4325.
[17] Marklund S, Chaudhary R, Marklund L, et al. Extensive mtDNA diversity in horses revealed by PCR-SSCP analysis[J]. Animal Genetics,1995,26(3):193-196.
[18] Jia X, Zhang Z, Liu Y, et al. Development and genetic mapping of SSR markers in foxtail millet[Setaria italica(L.)P. Beauv.][J].Theoretical&Applied Genetics,2009,118(4):821-829.
[19] Jia X P, Shi Y S, Song Y C, et al. Development of EST-SSR in foxtail millet(Setaria italica)[J]. Genetic Resources&Crop Evolution,2007,54(2):233-236.
[20] Fang X, Dong K, Wang X, et al. A high density genetic map and QTL for agronomic and yield traits in Foxtail millet[Setaria italica(L.)P. Beauv.][J]. Bmc Genomics,2016,17(1):336.
[21] Zhang G, Liu X, Quan Z, et al. Genome sequence of foxtail millet(Setaria italica)provides insights into grass evolution and biofuel potential[J]. Nature Biotechnology, 2012,30(6):549-554.
[22] Kosambi D D. The Estimation of map distances from recombination values[J]. Annals of Human Genetics,2012,12(1):172-175.
[23]刘仁虎,孟金陵. MapDraw,在Excel中绘制遗传连锁图的宏[J].遗传,2003,25(3):317-321.
[24] Liu B, Wang Y, Zhai W, et al. Development of InDel markers for Brassica rapa based on whole-genome re-sequencing[J]. Theoretical&Applied Genetics, 2013,126(1):231-239.
[25] Jander G, Norris S R, Rounsley S D, et al. Arabidopsis map-based cloning in the post-genome era[J]. Plant Physiology,2002,129(2):440-50.
[26]刘玉林.大豆遗传图谱构建及重要农艺性状QTL定位[D].北京:中国农业科学院,2011.
[27]田希辉,于拴仓,苏同兵,等.一个新的白菜苗期TuMV-C4抗性主效QTL定位及连锁分子标记开发[J].华北农学报,2014,29(6):1-5.
[28]王岩,付新民,高冠军,等.分子标记辅助选择改良优质水稻恢复系明恢63的稻米品质[J].分子植物育种,2009,7(4):661-665.
[29]闻可心,刘雪梅.AP2功能基因在植物花发育中的重要作用[J].生物技术通报,2010(2):1-7.
[30]赵奇,王台,魏小弟.AP2基因在高等植物花器官发育中的作用概述[J].热带农业科学,2005,25(3):50-56.
[31]张妍,刘瀛,孙丰宾,等.白桦APETALA2(AP2)转录因子基因的分离及其表达[J].林业科学研究,2012,25(2):254-260.
[32] Jofuku K D, den Boer B G, Van M M, et al. Control of Arabidopsis flower and seed development by the homeotic gene APETALA2[J].Plant Cell,1994, 6(9):1211-1225.
[33] PréM, Atallah M, Champion A, et al. The AP2/ERF domain transcription factor ORA59 integrates jasmonic acid and ethylene signals in plant defense[J]. Plant Physiology,2008,147(3):1347-1357.
[34]秦捷,王武,左开井,等. AP2基因家族的起源和棉花AP2转录因子在抗病中的作用[J].棉花学报,2005,17(6):366-370.
[35]李雅韵,廖文彬,王斌,等.干旱胁迫下木薯AP2转录因子的荧光定量PCR分析[J].广东农业科学,2014,41(10):126-131.
[36]吴慧敏.水稻AP2/EREBP转录因子基因OsASIE1抗逆功能分析[D].北京:中国农业科学院,2011.
[37]陈旭君,郭泽建.增加表达AP2/EREBP类转录因子OPBP1基因提高烟草的抗逆能力[A].中国植物病理学会2004年学术年会论文集[C].2004.
[38] Manzanares C, Barth S, Thorogood D, et al. A gene encoding a DUF247 domain protein co-segregates with the S selfincompatibility locus in perennial ryegrass[J]. Molecular Biology&Evolution,2016,33(4):870-884.
[39]张浩,汤沙,李迎涛,等.谷子窄颖花突变体sins1的表型分析和突变基因定位[J].植物遗传资源学报,2017,18(3):538-545.
[40] Fan X, Tang S, Zhi H, et al. Identification and fine mapping of SiDWARF3(D3), a pleiotropic locus controlling environmentindependent dwarfism in foxtail millet[J]. Crop Science,2017,57(5).
[41] Li W, Tang S, Zhang S, et al. Gene mapping and functional analysis of the novel leaf color gene SiYGL1 in foxtail millet[Setaria italica(L.)P. Beauv][J]. Physiologia Plantarum,2015,157(1):24-37.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |