背部循经推拿手法对亚健康模型大鼠海马神经元凋亡相关基因Bcl-2、Bax及Casepase-3表达的影响
|
摘要
目的:观察背部循经推拿对亚健康模型大鼠海马神经元凋亡相关基因Bcl-2、Bax及Casepase-3蛋白表达的影响。方法:60只wistar大鼠随机分为正常组、模型组和推拿干预组,后两组予束缚应激,推拿干预组予背部循经推拿。实验结束后,采用免疫组化法检测3组大鼠海马组织Bcl-2、Bax及Casepase-3蛋白表达,实时荧光定量PCR方法检测Casepase-3 mRNA的表达情况。结果:模型组大鼠海马组织Bcl-2蛋白表达下降、Bax与Caspase-3蛋白表达上升,与空白组比较,差异有显著性(P<0.05);推拿干预组海马组织Bcl-2蛋白表达上调、Bax蛋白表达下调、Caspase-3蛋白及Caspase-3 mRNA表达均上升,与模型组比较,差异有显著性(P<0.05)。结论:背部循经推拿手法可以通过抑制Bax与Caspase-3蛋白及Caspase-3 mRNA表达、上调Bcl-2蛋白表达,从而发挥对慢性应激所致亚健康状态的脑神经保护作用。
Objective:To observe the effect of Chinese massaging along the meridians on the back on expressions of apoptosis related genes Bcl-2, Bax and Casepase-3 in hippocampal neurons of sub-health model rats. Methods:Sixty Wister rats were divided into normal feeding group,model group and Chinese Tuina intervention group. The latter two groups were treated with restraint stress. Besides,the Tuina group used Tuina. Expressions of Bcl-2, Bax and Casepase-3 protein in 3 groups of rats were detected by immunohistochemistiy. The expression of Casepase-3 mRNA was detected by real-time quantitative PCR. Results:The expression of Bcl-2 protein in hippocampus of model rats decreased and the expressions of Bax and Caspase-3 protein increased. The difference was significant comparing with control group(P<0.05). In the Tuina intervention group, expressions of Bcl-2 protein, Caspase-3 protein and Caspase-3 mRNA increased and the expression of Bax protein decreased. Compared with the model group, the difference was significant(P<0.05). Conclusion:Chinese massaging along the meridians on the back can inhibit the expressions of Bax,Caspase-3 protein,Caspase-3 mRNA and upregulate the expression of Bcl-2 protein so as to exert the effect on chronic stress induced sub health state.
Objective:To observe the effect of Chinese massaging along the meridians on the back on expressions of apoptosis related genes Bcl-2, Bax and Casepase-3 in hippocampal neurons of sub-health model rats. Methods:Sixty Wister rats were divided into normal feeding group,model group and Chinese Tuina intervention group. The latter two groups were treated with restraint stress. Besides,the Tuina group used Tuina. Expressions of Bcl-2, Bax and Casepase-3 protein in 3 groups of rats were detected by immunohistochemistiy. The expression of Casepase-3 mRNA was detected by real-time quantitative PCR. Results:The expression of Bcl-2 protein in hippocampus of model rats decreased and the expressions of Bax and Caspase-3 protein increased. The difference was significant comparing with control group(P<0.05). In the Tuina intervention group, expressions of Bcl-2 protein, Caspase-3 protein and Caspase-3 mRNA increased and the expression of Bax protein decreased. Compared with the model group, the difference was significant(P<0.05). Conclusion:Chinese massaging along the meridians on the back can inhibit the expressions of Bax,Caspase-3 protein,Caspase-3 mRNA and upregulate the expression of Bcl-2 protein so as to exert the effect on chronic stress induced sub health state.
引文
[1] 于春泉,张伯礼,马寰.亚健康状态主要类型及流行病学调查现状[J].天津中医学院学报,2005,2(24):91-93.
[2] 伦轼芳,庞军,雷龙鸣,等.推拿疗法对亚健康状态人群心身症状的调理作用[J].中国临床康复,2005,9(40):100-101.
[3] 雷龙鸣,曾湘玲,伦轼芳,等.背部循经推拿对39例心理性亚健康患者的调治作用[J].中医杂志,2010(2):142-144.
[4] 雷龙鸣,伦轼芳,黄锦军,等.薄荷精油循经推拿调治社会应激性亚健康抑郁状态93例[J].中华中医药杂志,2011,26(2):411-413.
[5] 王天芳,陈易新,季绍良,等.慢性束缚致慢性疲劳动物模型的研制及其行为学观察[J].中国中医基础医学杂志,1999,5(5):25-29.
[6] 张幸娟,严灿,高敏.中医药与心理应激亚健康状态的调节[J].中医杂志,2002,43(9):647-648.
[7] 陈鹏,张玉春,张延英,等.安全艾灸器艾灸慢性疲劳模型大鼠五脏背俞穴对其慢性疲劳表现以及行为学的影响[J].甘肃中医学院学报,2010,27(3):22-25.
[8] 李晶晶,李晓虹,王少贤,等.逍遥散对肝郁脾虚证实验大鼠的治疗作用[J].吉林中医药,2009,29(10):901-903.
[9] 梁媛,郭晓玲,岳广欣,等.逍遥散调节肝郁脾虚大鼠中枢AMPA受体及相关蛋白机制研究[J].中华中医药杂志,2011,26(1):61-65.
[10] 姜幼明,张汉霆,陈家旭. 逍遥散对慢性束缚应激肝郁脾虚证模型大鼠杏仁核Bax和Bcl-2蛋白表达的影响[J].中华中医药杂志,2012,27(4):882-885.
[11] 岳利峰,丁杰,陈家旭,等. 肝郁脾虚证大鼠模型的建立与评价[J].北京中医药大学学报,2008,31(6):396-400.
[12] 包伍叶,焦爽,卢峻,等. 电针对慢性应激抑郁大鼠学习记忆与海马神经元损伤的影响[J].针刺研究,2014,39(2):136-141.
[13] 武丽,张曼,张丽萍,等.加味温胆汤对抑郁模型大鼠CA3区神经元超微结构的影响[J].山东中医药大学学报,2013,37(5):434-436.
[14] 邵淑红,李尊岭,潘芳,等.急、慢性应激对大鼠海马BDNF表达的影响[J].基础医学与临床,2012,32(4):428-432.
[15] Pavic L,Gregurek R,Rados M.Smaller right hippocampus in war veterans with posttraumatic stress disorder[J].Psychiatry Res,2007,154:191-198.
[16] 陈兴华,杨海涛,唐纯志,等.艾灸五脏背俞穴对慢性疲劳模型大鼠海马神经元形态结构的影响[J].辽宁中医杂志,2009,36(5):822-824.
[17] 李丽萍,毕颖.针灸对慢性应激抑郁模型大鼠脑源性神经营养因子的影响[J].中华中医药学刊,2008,26(10):2287-2290.
[18] 刘瑾彦,娄淑杰,陈佩杰.运动与中枢海马神经元再生研究进展[J].中国运动医学杂志,2008,27(3):401-404.
[19] 马强,王静,刘洪涛,等.体力运动减缓慢性应激对海马的损伤作用[J].生理学报,2002,54(5):427-430.
[20] 屈波,蔡光先,刘柏炎,等.超微六味地黄汤对孕期被动吸烟胎鼠脑Bax、Bcl-2和Casepase-3的影响[J].实用预防医学,2008,15(6):1679-1682.
[21] 李琴,郭云良,李霞,等.胡黄连苷对大鼠脑缺血/再灌注损伤Caspase-3和PARP表达的影响[J].中国药理学通报,2010,26(3):342-345.
[22] Mazumder S, Plesca D, Almasan A. Caspase-3 activation is a critical determinant of genotoxic stress-induced apoptosis [J]. Methods Mol Biol, 2008, 414: 13-21.
[23] 傅锦华,刘勇,王清勇,等.舒肝解郁胶囊对抑郁模型大鼠海马神经元凋亡及脑组织Caspase-3蛋白表达的影响[J].中南大学学报,2012,37(12):1198-1203.
[2] 伦轼芳,庞军,雷龙鸣,等.推拿疗法对亚健康状态人群心身症状的调理作用[J].中国临床康复,2005,9(40):100-101.
[3] 雷龙鸣,曾湘玲,伦轼芳,等.背部循经推拿对39例心理性亚健康患者的调治作用[J].中医杂志,2010(2):142-144.
[4] 雷龙鸣,伦轼芳,黄锦军,等.薄荷精油循经推拿调治社会应激性亚健康抑郁状态93例[J].中华中医药杂志,2011,26(2):411-413.
[5] 王天芳,陈易新,季绍良,等.慢性束缚致慢性疲劳动物模型的研制及其行为学观察[J].中国中医基础医学杂志,1999,5(5):25-29.
[6] 张幸娟,严灿,高敏.中医药与心理应激亚健康状态的调节[J].中医杂志,2002,43(9):647-648.
[7] 陈鹏,张玉春,张延英,等.安全艾灸器艾灸慢性疲劳模型大鼠五脏背俞穴对其慢性疲劳表现以及行为学的影响[J].甘肃中医学院学报,2010,27(3):22-25.
[8] 李晶晶,李晓虹,王少贤,等.逍遥散对肝郁脾虚证实验大鼠的治疗作用[J].吉林中医药,2009,29(10):901-903.
[9] 梁媛,郭晓玲,岳广欣,等.逍遥散调节肝郁脾虚大鼠中枢AMPA受体及相关蛋白机制研究[J].中华中医药杂志,2011,26(1):61-65.
[10] 姜幼明,张汉霆,陈家旭. 逍遥散对慢性束缚应激肝郁脾虚证模型大鼠杏仁核Bax和Bcl-2蛋白表达的影响[J].中华中医药杂志,2012,27(4):882-885.
[11] 岳利峰,丁杰,陈家旭,等. 肝郁脾虚证大鼠模型的建立与评价[J].北京中医药大学学报,2008,31(6):396-400.
[12] 包伍叶,焦爽,卢峻,等. 电针对慢性应激抑郁大鼠学习记忆与海马神经元损伤的影响[J].针刺研究,2014,39(2):136-141.
[13] 武丽,张曼,张丽萍,等.加味温胆汤对抑郁模型大鼠CA3区神经元超微结构的影响[J].山东中医药大学学报,2013,37(5):434-436.
[14] 邵淑红,李尊岭,潘芳,等.急、慢性应激对大鼠海马BDNF表达的影响[J].基础医学与临床,2012,32(4):428-432.
[15] Pavic L,Gregurek R,Rados M.Smaller right hippocampus in war veterans with posttraumatic stress disorder[J].Psychiatry Res,2007,154:191-198.
[16] 陈兴华,杨海涛,唐纯志,等.艾灸五脏背俞穴对慢性疲劳模型大鼠海马神经元形态结构的影响[J].辽宁中医杂志,2009,36(5):822-824.
[17] 李丽萍,毕颖.针灸对慢性应激抑郁模型大鼠脑源性神经营养因子的影响[J].中华中医药学刊,2008,26(10):2287-2290.
[18] 刘瑾彦,娄淑杰,陈佩杰.运动与中枢海马神经元再生研究进展[J].中国运动医学杂志,2008,27(3):401-404.
[19] 马强,王静,刘洪涛,等.体力运动减缓慢性应激对海马的损伤作用[J].生理学报,2002,54(5):427-430.
[20] 屈波,蔡光先,刘柏炎,等.超微六味地黄汤对孕期被动吸烟胎鼠脑Bax、Bcl-2和Casepase-3的影响[J].实用预防医学,2008,15(6):1679-1682.
[21] 李琴,郭云良,李霞,等.胡黄连苷对大鼠脑缺血/再灌注损伤Caspase-3和PARP表达的影响[J].中国药理学通报,2010,26(3):342-345.
[22] Mazumder S, Plesca D, Almasan A. Caspase-3 activation is a critical determinant of genotoxic stress-induced apoptosis [J]. Methods Mol Biol, 2008, 414: 13-21.
[23] 傅锦华,刘勇,王清勇,等.舒肝解郁胶囊对抑郁模型大鼠海马神经元凋亡及脑组织Caspase-3蛋白表达的影响[J].中南大学学报,2012,37(12):1198-1203.