泰山松花粉多糖对鸡感染vvIBDV诱导的免疫抑制的免疫调理作用
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摘要
鸡传染性法氏囊超强毒株(vvIBDV)可引起鸡群高死亡率及严重的免疫抑制,给养禽业带来巨大的经济损失。为了探究泰山松花粉多糖(TPPPS)对鸡传染性法氏囊病(IBD)的免疫调理作用,本研究以IBDV GX8/99超强毒株经鼻腔感染19日龄SPF鸡,并分别于感染前、后连续14d口服饲喂TPPPS,同时设置IBD疫苗对照组,病毒对照组和健康对照组,分别对各组试验鸡体质量及法氏囊指数、法氏囊带毒量及抗体变化,以及其他相关免疫学指标进行检测比较。结果显示,连续14d口服TPPPS后,试验鸡体质量及法氏囊抑制程度得到明显改善,法氏囊带毒量减少,法氏囊损伤程度降低,IBD抗体分泌提前、分泌量增加,IL-2、IL-4细胞因子分泌水平提高,T淋巴细胞转化率、CD4+和CD8+T细胞数增加,ND抗体水平提高,其中TPPPS预防组的各项检测指标均高于TPPPS治疗组和IBD疫苗组。结果表明,TPPPS对vvIBDV诱导的鸡群免疫抑制具有一定的免疫调理作用,且以在病毒接种前口服TPPPS发挥的免疫调理作用最显著,TPPPS可以作为免疫增强剂用于防控vvIBDV的早期感染,降低其免疫抑制程度,减少经济损失。
Very virulent infectious bursal disease virus(vvIBDV)can cause high mortality and severe immunosuppression of chickens and bring huge economic losses to poultry industry.To explore the immunomodulation of Taishan Pinus massoniana pollen polysaccharide(TPPPS)on immunosuppressed chickens infected with infectious bursal disease(IBD),19-day-old SPF chickens were infected with very virulent strain of IBDV GX8/99 by nasal cavity,and orally successive TPPPS administration for 14 days before and after infection,IBD vaccine control group,virus control group and healthy control group were set at the same time,then the body weight,bursa index,the dynamic changes of viremia,antibody secretion and immunological parameters were monitored.The results showed that after continuously oral administration of TPPPS for 14 days,as characterized by increase in body weight and bursa index,decline in viremia of bursal,decrease in bursal injury,IBD antibody secretion in advance and enhance,also increase in cytokines(IL-2,IL-4)secretion,lymphocyte transformation rate,CD4+and CD8+T lymphocytes,ND antibody level.Wherein,TPPPS behaved more significant immunomodulation in the TPPPS prevention group.These results indicated TPPPS had immune enhancement to vvIBDV-induced immune suppression of chickens,and the effect of oral administration of TPPPS before inoculation was most significant.Thus,TPPPS can be used as an immunoenhancer to reduce the immune suppression and economic losses for vvIBDV prevention and control in early infection.
Very virulent infectious bursal disease virus(vvIBDV)can cause high mortality and severe immunosuppression of chickens and bring huge economic losses to poultry industry.To explore the immunomodulation of Taishan Pinus massoniana pollen polysaccharide(TPPPS)on immunosuppressed chickens infected with infectious bursal disease(IBD),19-day-old SPF chickens were infected with very virulent strain of IBDV GX8/99 by nasal cavity,and orally successive TPPPS administration for 14 days before and after infection,IBD vaccine control group,virus control group and healthy control group were set at the same time,then the body weight,bursa index,the dynamic changes of viremia,antibody secretion and immunological parameters were monitored.The results showed that after continuously oral administration of TPPPS for 14 days,as characterized by increase in body weight and bursa index,decline in viremia of bursal,decrease in bursal injury,IBD antibody secretion in advance and enhance,also increase in cytokines(IL-2,IL-4)secretion,lymphocyte transformation rate,CD4+and CD8+T lymphocytes,ND antibody level.Wherein,TPPPS behaved more significant immunomodulation in the TPPPS prevention group.These results indicated TPPPS had immune enhancement to vvIBDV-induced immune suppression of chickens,and the effect of oral administration of TPPPS before inoculation was most significant.Thus,TPPPS can be used as an immunoenhancer to reduce the immune suppression and economic losses for vvIBDV prevention and control in early infection.
引文
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[21]王灵娟,高雪丽,隋欣,等.传染性法氏囊病病毒对SPF雏鸡免疫器官T细胞增殖能力及亚型的影响[J].中国家禽,2013,35(13):12-15.
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[23]刘爵,韦莉,姚炜光,等.传染性法氏囊病病毒超强毒感染SPF鸡免疫器官中CD4+和CD8+T淋巴细胞动态分布[J].中国兽医学报,2002,22(1):15-18.
[24]高海,刘开永,李秀岚,等.中药复方连翘对感染法氏囊病毒雏鸡免疫功能的影响[J].畜牧兽医学报,2009,40(1):109-116.
[25]杜继红,朱华,崔小七,等.板蓝根颗粒对鸡传染性法氏囊病的防制效果研究[J].中国畜牧兽医,2014,41(6):236-240.
[26]冯善祥.紫锥菊复合物对雏鸡人工感染新城疫病毒效果观察[J].中国畜牧兽医,2011,38(4):225-228.
[27]冯善祥.复方金银花对雏鸡法氏囊强毒抑制效果观察试验[J].中国动物检疫,2010,27(11):55-57.
[2]MVLLER H,ISLAM M R,RAUE R.Research on infectious bursal disease-the past,the present and the future[J].Vet Microbiol,2003,97(1/2):153-165.
[3]HAFEZ H M,PRUSAS C,RAUE R.Very virulent infectious bursal disease virus(vvIBDV)in vaccinated broiler flock:course of the disease,identification and characterisation of isolated strain[J].BMC Res Nat,2003,3(1):1-6.
[4]朱子洁,尤永君,苏霞,等.鸡传染性法氏囊病疫苗对SPF鸡ND免疫效果的影响[J].动物医学进展,2010,31(s1):132-135.
[5]王如涛,吴绵斌,林建平,等.植物多糖分离提取技术的研究进展[J].中国生物工程杂志,2013,33(7):118-123.
[6]易凤英,刘素纯,李佳莲,等.冠突散囊菌中多糖物质的研究[D].湖南长沙:湖南农业大学,2012.
[7]曲亭和.泰山松花粉多糖微囊对REV免疫抑制鸡的免疫调理作用[D].山东泰安:山东农业大学,2014.
[8]朱丽君,胡莉萍,董雯雯,等.泰山马尾松花粉多糖对ALV-J不同接种途径诱导的鸡群免疫抑制的免疫调理作用[J].中国兽医学报,2018,38(3):549-555,569.
[9]YU C,WEI K,LIU L,et al.Taishan Pinus massoniana pollen polysaccharide inhibits subgroup J avian leucosis virus infection by directly blocking virus infection and improving immunity[J].Sci Rep,2017,7:44353.
[10]YANG S,WEI K,JIA F,et al.Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro[J].PLoS One,2015,10(3):e0115638.
[11]MWANZA M,KAMETLER L,BONAI A,et al.The cytotoxic effect of fumonisin B1and ochratoxin A on human and pig lymphocytes using the methyl thiazol tetrazolium(MTT)assay[J].Mycotoxin Res,2009,25(4):233-238.
[12]陈悦,刘兴友.鸡病毒性免疫抑制病的流行病学研究进展[J].中国畜牧兽医,2010,37(2):192-194.
[13]章振华,李林,景小冬,等.鸡传染性法氏囊病免疫复合物疫苗对SPF雏鸡的免疫效果[J].动物医学进展,2015,36(10):39-43.
[14]CHEN L,RAN M J,SHAN X X,et al.BAFF enhances B-cell-mediated immune response and vaccineprotection against a very virulent IBDV in chickens[J].Vaccine,2009,27(9):1393-1399.
[15]JACKWOOD D J,SOMMER-WAGNER S E.Amino acids contributing to antigenic drift in the infectious bursal disease Birnavirus(IBDV)[J].Virology,2011,409(1):33.
[16]邓治邦,曾元根,杨丽君.传染性法氏囊病康复鸡群的免疫变化[J].动物医学进展,2003,24(1):116-117.
[17]OOI V E,LIU F.Immunomodulation and anti-cancer activity of polysaccharide-protein complexes[J].Curr Med Chem,2000,7(7):715-729.
[18]罗燕,邵永斌,谷新利,等.淫羊藿多糖提取工艺的优化及对小鼠细胞免疫功能的影响[J].黑龙江畜牧兽医,2009,52(9):104-106.
[19]吕小兰,赖小平,郭惠,等.牛膝多糖研究进展[J].实用临床医学,2005,6(2):137-138.
[20]姜国均,马清河,黄军,等.中药"增免散"对免疫抑制鸡免疫功能的影响[J].中国农学通报,2006,22(7):12-15.
[21]王灵娟,高雪丽,隋欣,等.传染性法氏囊病病毒对SPF雏鸡免疫器官T细胞增殖能力及亚型的影响[J].中国家禽,2013,35(13):12-15.
[22]ZINKERNAGEL R M.Immunology taught by viruses[J].Science,1996,271(5246):173-178.
[23]刘爵,韦莉,姚炜光,等.传染性法氏囊病病毒超强毒感染SPF鸡免疫器官中CD4+和CD8+T淋巴细胞动态分布[J].中国兽医学报,2002,22(1):15-18.
[24]高海,刘开永,李秀岚,等.中药复方连翘对感染法氏囊病毒雏鸡免疫功能的影响[J].畜牧兽医学报,2009,40(1):109-116.
[25]杜继红,朱华,崔小七,等.板蓝根颗粒对鸡传染性法氏囊病的防制效果研究[J].中国畜牧兽医,2014,41(6):236-240.
[26]冯善祥.紫锥菊复合物对雏鸡人工感染新城疫病毒效果观察[J].中国畜牧兽医,2011,38(4):225-228.
[27]冯善祥.复方金银花对雏鸡法氏囊强毒抑制效果观察试验[J].中国动物检疫,2010,27(11):55-57.