脊髓损伤大鼠模型造模方式选择的网状Meta分析
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摘要
背景:研究脊髓损伤的病理生理、解剖结构等的改变,其前提条件是必须建立一个安全有效且临床相似度高的脊髓损伤大鼠模型。随着研究的进展,基于不同目的各种相关的造模方式层出不穷,对后来的研究者在造模方式的选择难免造成些许干扰。目的:运用网状Meta分析的方法,比较脊髓损伤挫伤型、压迫型、缺血损伤型、横断型各造模方式的有效性和死亡率的排序,为脊髓损伤大鼠模型的造模方式提供有力证据。方法:通过检索截止时间为2018-03-11包括Pub Med、中国知网、重庆维普,万方4个数据库所有关于脊髓损伤造模方式的文献,对文献进行筛选,提取最终纳入文献数据,运用Stata、Winbug软件进行网状分析,得出排序结果。结果与结论:最终17篇文献纳入研究,共506只SpragueDawley(SD)大鼠,脊髓损伤造模方式有效性排序:横断型>挫伤型>压迫型>缺血损伤型;造模方式死亡率从高到低的排序:横断型>挫伤型>压迫型>缺血损伤型。
BACKGROUND: A safe and effective spinal cord injury rat model with high clinical similarity is important for the studies on changes in the pathophysiology and anatomy of spinal cord injury. There are various modeling methods, so choosing which one still remains controversial. OBJECTIVE: To compare the efficacy and mortality of various models of spinal cord injury contusion type, compression type, ischemic injury type, and transverse type using a meta-analysis so as to provide evidence fo establishing rat models of spinal cord injury.METHODS: Databases of PubMed, CNKI, VIP and WanFang were searched for the literature concerning spinal cord injury modeling methods published before March 11, 2018. The literature screening and data extraction were conducted. Mesh analysis was performed on Stata and Winbug software to obtain the efficacy results. RESULTS AND CONCLUSION: A total of 17 eligible articles were included, involving 506 Sprague-Dawley rats. The order of the effectiveness of spinal cord injury model was as follows: transverse > contusion > compression > ischemic injury. The order of mortality was as follows: transverse > contusion > compression > ischemic injury.
BACKGROUND: A safe and effective spinal cord injury rat model with high clinical similarity is important for the studies on changes in the pathophysiology and anatomy of spinal cord injury. There are various modeling methods, so choosing which one still remains controversial. OBJECTIVE: To compare the efficacy and mortality of various models of spinal cord injury contusion type, compression type, ischemic injury type, and transverse type using a meta-analysis so as to provide evidence fo establishing rat models of spinal cord injury.METHODS: Databases of PubMed, CNKI, VIP and WanFang were searched for the literature concerning spinal cord injury modeling methods published before March 11, 2018. The literature screening and data extraction were conducted. Mesh analysis was performed on Stata and Winbug software to obtain the efficacy results. RESULTS AND CONCLUSION: A total of 17 eligible articles were included, involving 506 Sprague-Dawley rats. The order of the effectiveness of spinal cord injury model was as follows: transverse > contusion > compression > ischemic injury. The order of mortality was as follows: transverse > contusion > compression > ischemic injury.
引文
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[3]牟广韬.脊髓损伤模型建立的研究进展[J].中国中西医结合外科杂志,2014,20(2):223-225.
[4]张军,陈晓,张荣佳,等.脊髓损伤动物模型的研究进展[J].中华全科医学,2017,15(3):501-504.
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[9]焦焦焦,蒋建农,都林,等.银杏叶提取物EGb761对大鼠脊髓损伤后神经损伤恢复的影响[J].实用临床医药杂志,2010,14(15):5-8.
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[14]李经李,黄李,吴海鹰,等.钳夹型急性大鼠脊髓损伤模型的建立与评价[J].昆明理工大学学报(自然科学版),2012,37(6):67-71+75.
[15]秦秦秦,王文孟,葛杜鹃,等.改良钳夹型脊髓损伤大鼠模型的建立及神经功能评价[J].西南军医,2014,16(01):1-4.
[16]孟孟孟.锌促进骨髓间充质干细胞修复大鼠脊髓缺血再灌注损伤的研究[D].长孟:吉林大学,2011.
[17]赵赵赵.人参皂甙Rb_1治疗大鼠脊髓缺血再灌注损伤的实验研究[D].长孟:吉林大学,2014.
[18]任小波,王桂华,路坦,等.脂氧素受体激动剂BML-111对脊髓缺血再灌注损伤模型大鼠的神经保护作用[J].中国组织工程研究,2016,20(18):2642-2647.
[19]林林林,刘文革.大鼠T_10脊髓半横切损伤模型的建立及饲养护理[J].福建医药杂志,2010,32(1):82-86.
[20]孟孟孟,巴迎孟,宋士娜,等.脊髓全横断大鼠模型的构建[J].中国组织工程研究与临床康复,2011,15(07):1215-1218.
[21]赵富生,武庚,武郭,等.大鼠脊髓楔型半切空洞模型的建立及评估[J].实验动物科学,2013,30(4):13-17.
[22]蔚洪恩,王孟芳,李鹏飞,等.大鼠脊髓T11段全横断损伤模型的建立及护理[J].山西医科大学学报,2007,38(6):484-486+577.
[23]张俐,安国尧,陈凯,等.夹闭腹主动脉和腰动脉的脊髓缺血再灌注损伤模型比较研究[J].医学研究生学报,2014,27(2):124-128.
[24]ALLEN A R.surgery of experimental lesion of spinal cord equivalent to crush injury of fracture dislocation.preliminary report.JAMA.1911;57:878-880.
[25]Ramer LM,Ramer MS,Bradbury EJ.Restoring function after spinal cord injury:towards clinical translation of experimental strategies.Lancet Neurol.2014;13(12):1241-1256.
[26]Tabakow P,Raisman G,Fortuna W,et al.Functional regeneration of supraspinal connections in a patient with transected spinal cord following transplantation of bulbar olfactory ensheathing cells with peripheral nerve bridging.Cell Transplant.2014;23(12):1631-1655.
[27]刘志恒.脊髓缺血再灌注损伤的实验研究[D].西安:第四军医大学,2010.
[28]李波霖,周宾宾,金昕,等.脊髓挫裂伤模型大鼠造模方式的选择:网状Meta分析[J].中国组织工程研究,2016,20(18):2731-2736.
[29]Bramer WM,Giustini D,Kramer BM.Comparing the coverage,recall,and precision of searches for 120 systematic reviews in Embase,MEDLINE,and Google Scholar:a prospective study.Syst Rev.2016;5:39.
[30]Halladay CW,Trikalinos TA,Schmid IT,et al.Using data sources beyond PubMed has a modest impact on the results of systematic reviews of therapeutic interventions.J Clin Epidemiol.2015;68(9):1076-1084.
[2]Furlan JC,Sakakibara BM,Miller WC,et al.Global incidence and prevalence of traumatic spinal cord injury.Can J Neurol Sci.2013;40(4):456-464.
[3]牟广韬.脊髓损伤模型建立的研究进展[J].中国中西医结合外科杂志,2014,20(2):223-225.
[4]张军,陈晓,张荣佳,等.脊髓损伤动物模型的研究进展[J].中华全科医学,2017,15(3):501-504.
[5]Lundh A,G?tzsche PC.Recommendations by Cochrane Review Groups for assessment of the risk of bias in studies.BMC Med Res Methodol.2008;8:22.
[6]郑郑郑,郭海龙,盛伟林,等.简易大鼠脊髓完全横断伤模型的建立[J].郑疆医科大学学报,2009,32(3):323-326.
[7]Long Y,Liang F,Gao C,et al.Hyperbaric oxygen therapy reduces apoptosis after spinal cord injury in rats.Int J Clin Exp Med.2014;7(11):4073-4081.
[8]郭郭,马勇,潘娅岚,等.脊髓康对大鼠脊髓损伤后神经功能恢复及脑源性神经营养因子表达的影响[J].中国康复理论与实践,2014,20(8):701-708.
[9]焦焦焦,蒋建农,都林,等.银杏叶提取物EGb761对大鼠脊髓损伤后神经损伤恢复的影响[J].实用临床医药杂志,2010,14(15):5-8.
[10]顾兵,金建波,李华南,王烁宇.大鼠创伤性脊髓损伤模型的建立[J].中国临床药理学与治疗学,2011,16(07):721-728.
[11]吕静.大鼠脊髓损伤模型的建立及行为学评分[D].长沙:中南大学,2011.
[12]祖波,尹宗生,张李.急性脊髓挫伤性模型的制备及观察[J].第四军医大学学报,2007,28(14):1264-1267.
[13]刘小康,徐建广,连小郑,等.大鼠钳夹式急性脊髓损伤模型的制备与评价[J].中国矫形外科杂志,2012,20(14):1318-1322.
[14]李经李,黄李,吴海鹰,等.钳夹型急性大鼠脊髓损伤模型的建立与评价[J].昆明理工大学学报(自然科学版),2012,37(6):67-71+75.
[15]秦秦秦,王文孟,葛杜鹃,等.改良钳夹型脊髓损伤大鼠模型的建立及神经功能评价[J].西南军医,2014,16(01):1-4.
[16]孟孟孟.锌促进骨髓间充质干细胞修复大鼠脊髓缺血再灌注损伤的研究[D].长孟:吉林大学,2011.
[17]赵赵赵.人参皂甙Rb_1治疗大鼠脊髓缺血再灌注损伤的实验研究[D].长孟:吉林大学,2014.
[18]任小波,王桂华,路坦,等.脂氧素受体激动剂BML-111对脊髓缺血再灌注损伤模型大鼠的神经保护作用[J].中国组织工程研究,2016,20(18):2642-2647.
[19]林林林,刘文革.大鼠T_10脊髓半横切损伤模型的建立及饲养护理[J].福建医药杂志,2010,32(1):82-86.
[20]孟孟孟,巴迎孟,宋士娜,等.脊髓全横断大鼠模型的构建[J].中国组织工程研究与临床康复,2011,15(07):1215-1218.
[21]赵富生,武庚,武郭,等.大鼠脊髓楔型半切空洞模型的建立及评估[J].实验动物科学,2013,30(4):13-17.
[22]蔚洪恩,王孟芳,李鹏飞,等.大鼠脊髓T11段全横断损伤模型的建立及护理[J].山西医科大学学报,2007,38(6):484-486+577.
[23]张俐,安国尧,陈凯,等.夹闭腹主动脉和腰动脉的脊髓缺血再灌注损伤模型比较研究[J].医学研究生学报,2014,27(2):124-128.
[24]ALLEN A R.surgery of experimental lesion of spinal cord equivalent to crush injury of fracture dislocation.preliminary report.JAMA.1911;57:878-880.
[25]Ramer LM,Ramer MS,Bradbury EJ.Restoring function after spinal cord injury:towards clinical translation of experimental strategies.Lancet Neurol.2014;13(12):1241-1256.
[26]Tabakow P,Raisman G,Fortuna W,et al.Functional regeneration of supraspinal connections in a patient with transected spinal cord following transplantation of bulbar olfactory ensheathing cells with peripheral nerve bridging.Cell Transplant.2014;23(12):1631-1655.
[27]刘志恒.脊髓缺血再灌注损伤的实验研究[D].西安:第四军医大学,2010.
[28]李波霖,周宾宾,金昕,等.脊髓挫裂伤模型大鼠造模方式的选择:网状Meta分析[J].中国组织工程研究,2016,20(18):2731-2736.
[29]Bramer WM,Giustini D,Kramer BM.Comparing the coverage,recall,and precision of searches for 120 systematic reviews in Embase,MEDLINE,and Google Scholar:a prospective study.Syst Rev.2016;5:39.
[30]Halladay CW,Trikalinos TA,Schmid IT,et al.Using data sources beyond PubMed has a modest impact on the results of systematic reviews of therapeutic interventions.J Clin Epidemiol.2015;68(9):1076-1084.