摘要
设计并合成了一种可检测Cu~(2+)的新双罗丹明类衍生物荧光增强型分子探针(RG1),并用核磁和高分辨质谱(HRMS)对其结构进行了表征。通过紫外-可见光谱和荧光光谱研究了该荧光分子探针对Cu~(2+)的识别性能,结果表明:在体积比为1∶1的乙腈-水(10mmol/L Tris-HCl,pH=7.2)溶液体系中,探针RG1本身无色且荧光很弱,加入Cu~(2+)后,探针在553nm处出现强的荧光发射峰,溶液颜色从无色变为粉红色,对Cu~(2+)表现出好的选择性。此外,该探针可在较宽的pH范围(5~10)内直接检测Cu~(2+)的浓度。结果表明,Cu~(2+)浓度在3.0×10~(-6)~1.5×10~(-5)mol/L范围内与探针的荧光强度呈良好的线性关系,Cu~(2+)检出限为3.31×10~(-7)mol/L。同时对河水进行加标回收实验,得到了良好的回收率。
A novel birhodamine-based fluorescent enhancement molecular probe(RG1)for the detection of Cu~(2+)was designed and synthesized.Its structure was characterized by ~1H NMR,~(13)C NMR and high resolution mass spectrometer(HRMS).The recognition properties of this fluorescent molecular probe for Cu~(2+)were studied by UV-Vis spectra and fluorescence spectra.The results showed that in acetonitrile-water(1∶1,V/V)solution the probe was colorless and the fluorescence intensity was very weak.Upon the addition of Cu~(2+),a new fluorescence emission peak at 553 nm appeared with increasing intensity evidently.The color of the solution changed from colorless to pink.Probe RG1 showed good selectivity to Cu~(2+).In addition,the probe could detect the concentration of Cu~(2+) directly in a wide range of pH(5-10).The concentration of Cu~(2+)was in good linearity with the fluorescence intensity of the probe RG1 in the range of 3.0×10~(-6)-1.5×10~(-5)mol/L with a detection limit of 3.31×10~(-7)mol/L.At the same time,the standard recovery experiments of river sample were carried out with satisfactory result.
引文
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