京海黄鸡FSHR基因组织表达谱及表达规律的研究
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摘要
为探讨促卵泡素受体(FSHR)基因mRNA在不同组织中的相对表达量和表达规律,探究其调控机制,根据原鸡FSHR基因的编码序列,跨内含子设计1对荧光定量引物,采用qRT-PCR技术检测,研究组织表达谱及其在性腺轴中的表达规律。结果表明:FSHR基因在卵巢和睾丸中高表达,极显著高于其他组织。FSHR基因的表达呈不断变化的趋势,在母鸡卵巢中出壳时最高,持续下降至16周龄时最低, 20周龄稍有回升;FSHR表达量在公鸡睾丸中也于出壳时最高,持续下降至16周龄时表达量最低。在公母鸡的垂体组织中,FSHR基因表达呈小幅上升,母鸡较公鸡上升幅度稍大,卵巢中的表达量略高于睾丸。这一研究为进一步研究FSHR基因的作用机制和表达调控提供了素材。
In order to explore the expression profile and expression pattern of FSHR gene and its possible mechanism of action, a pair of fluorescent quantitative PCR primers of FSHR was design across introns. qRT-PCR was used to detect the tissue expression profiles and expression patterns of FSHR. The results showed that FSHR gene achieved the highest expression in ovarian and testicular which was significantly higher than that in other tissue(P<0.01). The expression of FSHR gene showed a trend of change, and was the highest in the hen's ovary at birth, the lowest at 16 weeks of age, and slightly increased at 20 weeks of age. The expression level of FSHR was also the highest in the testis of male chicken at birth, the lowest at 16 weeks of age. The expression of FSHR gene increased slightly in the pituitary tissue of male and female chickens, and was slightly higher in the females compared with the males, and the ovarian expression level was slightly higher than the testicle. This study investigated the expression profile and expression pattern of FSHR gene in Jinghai Yellow chicken, and provided materials for further study on the mechanism of action and expression regulation of FSHR gene.
In order to explore the expression profile and expression pattern of FSHR gene and its possible mechanism of action, a pair of fluorescent quantitative PCR primers of FSHR was design across introns. qRT-PCR was used to detect the tissue expression profiles and expression patterns of FSHR. The results showed that FSHR gene achieved the highest expression in ovarian and testicular which was significantly higher than that in other tissue(P<0.01). The expression of FSHR gene showed a trend of change, and was the highest in the hen's ovary at birth, the lowest at 16 weeks of age, and slightly increased at 20 weeks of age. The expression level of FSHR was also the highest in the testis of male chicken at birth, the lowest at 16 weeks of age. The expression of FSHR gene increased slightly in the pituitary tissue of male and female chickens, and was slightly higher in the females compared with the males, and the ovarian expression level was slightly higher than the testicle. This study investigated the expression profile and expression pattern of FSHR gene in Jinghai Yellow chicken, and provided materials for further study on the mechanism of action and expression regulation of FSHR gene.
引文
[1] YOU S,BRIDGHAM J T,FOSTER D N,et al.Characterization of the chicken follicle-stimulating hormone receptor (cFSHR) complementary deoxyribonucleic acid,and expression of cFSHR messenger ribonucleic acid in the ovary [J].Biol Reprod,1996,55(5):1055-1062.
[2] 相德才,王春光,叶绍辉.鸡FSHR基因研究进展 [J].畜牧兽医科技信息,2014(6):4-6.
[3] SIMONI M,GROMOLL J,NIESCHLAG E.The follicle-stimulating hormone receptor:biochemistry,molecular biology,physiology,and pathophysiology [J].Endocrine Reviews,1997,18(6):739-773.
[4] JAMESON J L,BECKER C B,LINDELL C M,et al.Human follicle-stimulating hormone beta-subunit gene encodes multiplemessenger ribonucleic acids [J].Mol Endocrinol,1988,2(9):806-815.
[5] SILVA J M,CHRISTOPHER A P.Effect of follicle-stimulatinghormone on steroid secretion and messenger ribonucleic acidsencoding cytochromes P450 aromatase and cholesterol side-chain cleavage in bovine granulosa cells in vitro [J].Biol Reprod,2000,62:186-191.
[6] GHARIB S D,WIERMAN M E,SHUPNIK M A,et al.Molecularbiology of the pituitary gonadotropins [J].Eedocr Rev,1990,11(1):177-199.
[7] 张跟喜.边鸡遗传多样性及Myostatin基因对生长和繁殖性状的遗传效应研究 [D].扬州:扬州大学,2010.
[8] 倪迎冬,赵茹茜,王亚菊,等.出雏至性成熟绍鸭卵巢FSH-R与LH-R mRNA 表达的变化 [J].南京农业大学学报,2004,27(2):64-67.
[9] 牛金涛.水牛FSHR基因与其表达检测的研究 [D].南宁:广西大学,2007.
[10] 李悦,计红,薛琳琳,等.不同培养时间对鸡卵泡颗粒细胞孕酮、雌激素分泌水平及FSHR、LHR基因表达的影响 [J].中国应用生理学杂志,2017,33(2):174-178.
[11] 陈蓉,应诗家,陈哲,等.AMH及其相关基因在鸭不同等级卵泡中的表达 [J].江苏农业学报,2018,34(1):87-92.
[12] SCARLET D,WALTER I,HLAVATY J,et al.Expression and immunolocalisation of follicle-stimulating hormone receptors in gonads of newborn and adult female horses [J].Reprod Fertil Dev,2016,28(9):1340-1348.
[13] 产舒恒,陈子璇,张戌园,等.FSH和LH及其受体基因在梅山猪公猪HPT轴的表达 [J].江苏农业科学,2018,46(1):23-26.
[14] AKAZOME Y,ABE T,MORI T.Differentiation of chicken gonad as an endocrine organ:expression of LH receptor,FSH receptor,cytochrome P450c17 and aromatase genes [J].Reproduction,2002,123:721-728.
[15] ZHANG C,SHIMADA K,SAITO N,et al.Expression of messenger ribonucleic acids of luteinizing hormone and follicle-stimulating hormone receptors in granulose and theca layers of chicken preovulatory follicles [J].Gen Comp Endocrinol,1997,105(3):402-409.
[16] 张响英,唐现文,王利刚,等.狮头鹅不同繁殖阶段FSHβ及其受体的表达 [J].中国畜牧杂志,2013,49(1):14-17.
[17] HUO S D,CHEN S E,LONG R J,et al.Protein and mRNA expression of follicle-stimulating hormone receptor and luteinizing hormone receptor during the oestrus in the yak (Bos grunniens) [J].Reproduction in Domestic Animals,2017,52(3):477-482.
[18] 李亨.家禽繁殖性状相关基因的遗传效应及组织表达规律研究 [D].扬州:扬州大学,2011.
[19] 陈兰,王亚男,陈福祥,等.FSHR基因多态性对京海黄鸡繁殖性状的遗传效应研究 [J].中国家禽,2018,40(16):19-23.
[2] 相德才,王春光,叶绍辉.鸡FSHR基因研究进展 [J].畜牧兽医科技信息,2014(6):4-6.
[3] SIMONI M,GROMOLL J,NIESCHLAG E.The follicle-stimulating hormone receptor:biochemistry,molecular biology,physiology,and pathophysiology [J].Endocrine Reviews,1997,18(6):739-773.
[4] JAMESON J L,BECKER C B,LINDELL C M,et al.Human follicle-stimulating hormone beta-subunit gene encodes multiplemessenger ribonucleic acids [J].Mol Endocrinol,1988,2(9):806-815.
[5] SILVA J M,CHRISTOPHER A P.Effect of follicle-stimulatinghormone on steroid secretion and messenger ribonucleic acidsencoding cytochromes P450 aromatase and cholesterol side-chain cleavage in bovine granulosa cells in vitro [J].Biol Reprod,2000,62:186-191.
[6] GHARIB S D,WIERMAN M E,SHUPNIK M A,et al.Molecularbiology of the pituitary gonadotropins [J].Eedocr Rev,1990,11(1):177-199.
[7] 张跟喜.边鸡遗传多样性及Myostatin基因对生长和繁殖性状的遗传效应研究 [D].扬州:扬州大学,2010.
[8] 倪迎冬,赵茹茜,王亚菊,等.出雏至性成熟绍鸭卵巢FSH-R与LH-R mRNA 表达的变化 [J].南京农业大学学报,2004,27(2):64-67.
[9] 牛金涛.水牛FSHR基因与其表达检测的研究 [D].南宁:广西大学,2007.
[10] 李悦,计红,薛琳琳,等.不同培养时间对鸡卵泡颗粒细胞孕酮、雌激素分泌水平及FSHR、LHR基因表达的影响 [J].中国应用生理学杂志,2017,33(2):174-178.
[11] 陈蓉,应诗家,陈哲,等.AMH及其相关基因在鸭不同等级卵泡中的表达 [J].江苏农业学报,2018,34(1):87-92.
[12] SCARLET D,WALTER I,HLAVATY J,et al.Expression and immunolocalisation of follicle-stimulating hormone receptors in gonads of newborn and adult female horses [J].Reprod Fertil Dev,2016,28(9):1340-1348.
[13] 产舒恒,陈子璇,张戌园,等.FSH和LH及其受体基因在梅山猪公猪HPT轴的表达 [J].江苏农业科学,2018,46(1):23-26.
[14] AKAZOME Y,ABE T,MORI T.Differentiation of chicken gonad as an endocrine organ:expression of LH receptor,FSH receptor,cytochrome P450c17 and aromatase genes [J].Reproduction,2002,123:721-728.
[15] ZHANG C,SHIMADA K,SAITO N,et al.Expression of messenger ribonucleic acids of luteinizing hormone and follicle-stimulating hormone receptors in granulose and theca layers of chicken preovulatory follicles [J].Gen Comp Endocrinol,1997,105(3):402-409.
[16] 张响英,唐现文,王利刚,等.狮头鹅不同繁殖阶段FSHβ及其受体的表达 [J].中国畜牧杂志,2013,49(1):14-17.
[17] HUO S D,CHEN S E,LONG R J,et al.Protein and mRNA expression of follicle-stimulating hormone receptor and luteinizing hormone receptor during the oestrus in the yak (Bos grunniens) [J].Reproduction in Domestic Animals,2017,52(3):477-482.
[18] 李亨.家禽繁殖性状相关基因的遗传效应及组织表达规律研究 [D].扬州:扬州大学,2011.
[19] 陈兰,王亚男,陈福祥,等.FSHR基因多态性对京海黄鸡繁殖性状的遗传效应研究 [J].中国家禽,2018,40(16):19-23.
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