藏鸡FST基因组织表达谱及时序表达谱的研究
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摘要
研究旨在揭示卵泡抑素(FST)基因在藏鸡不同组织及发育阶段中的表达特性。选取1、42、70、120、150日龄藏鸡公母各5只,分别采集垂体、下丘脑、胸肌、腿肌组织样本,利用qRT-PCR技术,对FST基因在所选组织中的表达水平进行测定,构建组织表达谱及时序表达谱并进行分析。结果显示:FST基因主要在藏鸡垂体组织中表达,发育前期公鸡中的表达水平较高,发育后期母鸡中的表达水平高于公鸡;与下丘脑、胸肌以及腿肌时序表达特性不同,FST基因在藏鸡垂体组织中的时序表达特性具有性别差异。研究结果为进一步了解FST基因的功能及物种多样性提供了参考,也为藏鸡的遗传育种提供了一定的理论基础。
The aim of this study was to reveal the expression characteristics of follistatin(FST) gene in different tissues and developmental stages of Tibetan chicken. The pituitary, hypothalamus, breast muscle and leg muscle were obtained from Tibetan chickens of 1, 42, 70, 120 and 150 days of age(5 cocks and 5 hens at each stage), respectively.Using qRT-PCR technology detected the expression level of FST gene, and tissue expression profiles and time series expression profiles were constructed and analyzed. The results showed that FST gene was mainly expressed in the pituitary tissues of Tibetan chickens, the expression level in cocks was higher than that in hens in the pre-developmental,the opposite of late development; The temporal expression characteristics of FST gene in pituitary tissue had male-female differences, which was different from expression in other tissues. This study provided references for further understanding of the function and species diversity of FST gene, and also laid a theoretical foundation for the genetic breeding of Tibetan chicken.
The aim of this study was to reveal the expression characteristics of follistatin(FST) gene in different tissues and developmental stages of Tibetan chicken. The pituitary, hypothalamus, breast muscle and leg muscle were obtained from Tibetan chickens of 1, 42, 70, 120 and 150 days of age(5 cocks and 5 hens at each stage), respectively.Using qRT-PCR technology detected the expression level of FST gene, and tissue expression profiles and time series expression profiles were constructed and analyzed. The results showed that FST gene was mainly expressed in the pituitary tissues of Tibetan chickens, the expression level in cocks was higher than that in hens in the pre-developmental,the opposite of late development; The temporal expression characteristics of FST gene in pituitary tissue had male-female differences, which was different from expression in other tissues. This study provided references for further understanding of the function and species diversity of FST gene, and also laid a theoretical foundation for the genetic breeding of Tibetan chicken.
引文
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[13]ZHANG Y Q,KANZAKI M,SHIBATA H,et al.Regulation of the expression of follistatin in rat hepatocytes[J].Biochimica Et Biophysica Acta Gene Structure&Expression,1997,1354(3):204-210.
[14]MATZUK M M,LU N,VOGEL H,et al.Multiple defects and perinatal death in mice deficient in follistatin[J].Nature,1995,374(6520):360-363.
[15]LEE S J,MCPHERRON A C.Regulation of myostatin activity and muscle growth[J].Proceedings of the National Academy of Sciences of the United States of America,2001,98(16):9306-9311.
[16]胡艳,徐文娟,刘宏祥,等.鸭发育早期下丘脑-垂体生长轴相关基因mRNA的表达特异性分析[J].中国农业科学,2013,46(17):3712-3720.
[17]杨孟伯,杨莉,王鲜忠,等.大足黑山羊卵泡发育与内分泌激素分析[J].中国草食动物科学,2009,29(2):8-11.
[2]PATEL K.Molecules in focus:Follistatin[J].International Journal of Biochemistry&Cell Biology,1998,30(10):1087-1093.
[3]SIDIS Y,MUKHERJEE A,KEUTMANN H,et al.Biological activity of follistatin isoforms and follistatin-like-3 is dependent on differential cell surface binding and specificity for activin,myostatin,and bone morphogenetic proteins[J].Endocrinology,2006,147(7):3586-3597.
[4]PHILLIPS D J,KRETSER D M D.Follistatin:A Multifunctional regulatory protein[J].Frontiers in Neuroendocrinology,1998,19(4):287-322.
[5]SAITO S,SIDIS Y,MUKHERJEE A,et al.Differential biosynthesis and intracellular transport of follistatin isoforms and follistatin-like-3[J].Endocrinology,2005,146(12):5052-5062.
[6]SCHNEYER A L,WANG Q F,Sidis Y,et al.Differential distribution of follistatin isoforms:application of a new FS315-specific immunoassay[J].The Journal of Clinical Endocrinology&Metabolism,2004,89(10):5067-5075.
[7]SINGH R,BRAGA M,PERVIN S.Regulation of brown adipocyte metabolism by myostatin/follistatin signaling[J].Frontiers in Cell and Developmental Biology,2014,2:60.
[8]SINGH R,BRAGA M,REDDY S T,et al.Follistatin targets distinct pathways to promote brown adipocyte characteristics in brown and white adipose tissues[J].Endocrinology,2017,158(5):1217-1230.
[9]ZHENG H,QIAO C,TANG R,et al.Follistatin N terminus differentially regulates muscle size and fat in vivo[J].Experimental&Molecular Medicine,2017,49(9):e377.
[10]LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCRand the 2(-Delta Delta C(T))method[J].Methods,2001,25(4):402-408.
[11]罗艳梅,张超,赵中权,等.大足黑山羊卵泡抑素cDNA的克隆、序列分析及组织表达[J].中国农业科学,2011,44(22):4700-4705.
[12]刘小飞,薛良义.大黄鱼Follistatin基因克隆及表达分析[J].生物学杂志,2012,29(6):5-9.
[13]ZHANG Y Q,KANZAKI M,SHIBATA H,et al.Regulation of the expression of follistatin in rat hepatocytes[J].Biochimica Et Biophysica Acta Gene Structure&Expression,1997,1354(3):204-210.
[14]MATZUK M M,LU N,VOGEL H,et al.Multiple defects and perinatal death in mice deficient in follistatin[J].Nature,1995,374(6520):360-363.
[15]LEE S J,MCPHERRON A C.Regulation of myostatin activity and muscle growth[J].Proceedings of the National Academy of Sciences of the United States of America,2001,98(16):9306-9311.
[16]胡艳,徐文娟,刘宏祥,等.鸭发育早期下丘脑-垂体生长轴相关基因mRNA的表达特异性分析[J].中国农业科学,2013,46(17):3712-3720.
[17]杨孟伯,杨莉,王鲜忠,等.大足黑山羊卵泡发育与内分泌激素分析[J].中国草食动物科学,2009,29(2):8-11.
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