番茄SlTCP7转录因子的克隆及表达分析
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摘要
TCP转录因子家族是植物中特有的一类转录因子,具有典型bHLH结构域,本研究从番茄(Solanum lycopersicum)中克隆了SlTCP7基因。生物信息学分析表明,SlTCP7基因开放阅读框长为726 bp,编码241个氨基酸残基,相对分子量为27.34 kD,理论等电点为9.63。qRT-RCR表达模式分析表明,SlTCP7基因在番茄茎组织中表达量最高,其可能参与腋芽发育及茎分枝形态建成;qRT-PCR分析结果表明SlTCP7在IAA、ABA、GA3、CTK处理后表达量下调;非生物胁迫结果表明,高盐和干旱都对SlTCP7基因的表达量有不同程度的诱导。研究结果初步证实SlTCP7参与番茄的茎分枝过程以及非生物逆境胁迫响应,为SlTCP7基因在番茄生长发育过程中的功能提供了科学依据。
TCP transcription factor family is a plant-specific transcription factor, which has a typically bHLH domain. In this study, a gene named SlTCP7 was cloned from Solanum lycopersicum. Bioinformatics analysis showed that the length of SlTCP7 ORF was 726 bp, encoding 241 amino acid residues. The relative molecular weight was27.34 kD and the isoelectric point was 9.63. qRT-PCR expression pattern analysis indicated that the expression of SlTCP7 gene was the highest in tomato stem, indicating that it might be involved in the development of tomato axillary bud development and stem branching morphogenesis. The results of qRT-PCR analysis revealed that the expression of SlTCP7 was down-regulated under the treatments of IAA, ABA, GA3, CTK. In addition, abiotic stress results suggested that the expression of SlTCP7 could be induced by salt stress and drought stress. The results preliminarily confirmed that SlTCP7 participated in stem branching and abiotic stress response of tomato, which provided a scientific basis for the function of SlTCP7 gene in tomato growth and development.
TCP transcription factor family is a plant-specific transcription factor, which has a typically bHLH domain. In this study, a gene named SlTCP7 was cloned from Solanum lycopersicum. Bioinformatics analysis showed that the length of SlTCP7 ORF was 726 bp, encoding 241 amino acid residues. The relative molecular weight was27.34 kD and the isoelectric point was 9.63. qRT-PCR expression pattern analysis indicated that the expression of SlTCP7 gene was the highest in tomato stem, indicating that it might be involved in the development of tomato axillary bud development and stem branching morphogenesis. The results of qRT-PCR analysis revealed that the expression of SlTCP7 was down-regulated under the treatments of IAA, ABA, GA3, CTK. In addition, abiotic stress results suggested that the expression of SlTCP7 could be induced by salt stress and drought stress. The results preliminarily confirmed that SlTCP7 participated in stem branching and abiotic stress response of tomato, which provided a scientific basis for the function of SlTCP7 gene in tomato growth and development.
引文
Aguilarmartínez J.A.,Pozacarrión C.,and Cubas P.,2007,Arabidopsis BRANCHED1 acts as an integrator of branching signals within axillary buds,Plant Cell,19(2):458-472
An J.,Guo Z.,Gou X.,and Li J.,2011,TCP1 positively regulates the expression of DWF4 in Arabidopsis thaliana,Plant Signal Behav.,6(8):1117-1118
Bai F.,and Schmidt R.J.,2012,TCP transcription factor,BRANCH ANGLE DEFECTIVE 1(BAD1),is required for normal tassel branch angle formation in maize,Proc.Natl.Acad.Sci.USA,109(30):12225-12230
Braun N.,De S.G.A.,Pillot J.P.,Boutet-Mercey S.,Dalmais M.,Antoniadi I.,Li X.,Maia-Grondard A.,Le S.C.,Bouteiller N.,Luo D.,Bendahmane A.,Turnbull C.,and Rameau C.,2012,The pea TCP transcription factor PsBRC1 acts downstream of strigolactones to control shoot branching,Plant Physiol.,158(1):225-238
Chapman M.A.,Tang S.,Draeger D.,Nambeesan S.,Shaffer H.,Barb J.G.,Knapp S.J.,and Burke J M.,2012,Genetic analysis of floral symmetry in van gogh's sunflowers reveals independent recruitment of CYCLOIDEA genes in the asteraceae,PLoS Genet.,8(3):e1002628
Citerne H.L.,Mller M.,and Cronk Q.C.B.,2000,Diversity of cycloidea-like genes in gesneriaceae in relation to floral symmetry,Anna.Bot.,86(1):167-176
Cubas P.,Vincent C.,and Coen E.,1999,An epigenetic mutation responsible for natural variation in floral symmetry,Nature,401(6749):157-161
Dai C.,Peng Z.S.,Yang Z.J.,Liao M.L.,Lu L.,Wei S.H.,Zhang L.,Luo Q.,and Tang H.F.,2017,Cloning and expression analysis of glucosyltransferase gene TaUGT73D1 in wheat,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(6):2048-2057(代畅,彭正松,杨在君,廖明莉,路璐,魏淑红,张莉,罗琴,唐海峰,2017,小麦糖基转移酶基因TaUGT73D1的克隆及表达分析,分子植物育种,15(6):2048-2057)
Danisman S.,Wal F.V.D.,Dhondt S.,Waites R.,Folter S.D.,Bimbo A.,Van Dijk A.D.J.,Muino J.M.,Cutri L.,Dornelas M.C.,Angenent G.C.,and Immink R.G.H.,2012,Arabidopsis classⅠand classⅡTCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically,Plant Physiol.,159(4):1511-1523
Doebley J.,Stec A.,and Gustus C.,1995,teosinte branched1 and the origin of maize:evidence for epistasis and the evolution of dominance,Genetics,141(1):333-346
Du L.X.,Hu Z.L.,Zhang J.L.,and Chen G.P.,2014,Cloning,bioinformatics and expression analysis of SlDP1 gene in tomato,Zhiwu Yanjiu(Bulletin of Botanical Research),34(1):68-74(杜利欣,胡宗利,张建苓,陈国平,2014,番茄SlDP1基因的克隆、生物信息学及表达特性分析,植物研究,34(1):68-74)
Finlayson S.A.,2007,Arabidopsis teosinte Branched1-like 1 regulates axillary bud outgrowth and is homologous to monocot Teosinte Branched1,Plant Cell Physiol.,48(5):667-677
Guo Z.X.,Fujioka S.,Blancaflor E.B.,Miao S.,Gou X.P.,and Li J.,2010,TCP1 modulates brassinosteroid biosynthesis by regulating the expression of the key biosynthetic gene DW-ARF4 in Arabidopsis thaliana,Plant Cell,22(4):1161-1173
Ji Z.R.,Gu Y.B.,Dong Q.L.,Chi F.M.,Qiao Z.,Xu C.N.,Zhang J.X.,and Zhou Z.S.,2015,Genome-wide identification and analysis of TCP gene family in grape,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(10):2194-2199(冀志蕊,谷彦冰,董庆龙,迟福梅,乔壮,徐成楠,张俊祥,周宗山,2015,葡萄TCP基因家族全基因组鉴定和分析,基因组学与应用生物学,35(10):2194-2199)
Jibran R.,Hunter D.A.,and Dijkwel P.P.,2013,Hormonal regulation of leaf senescence through integration of developmental and stress signals,Plant Mol.Biol.,82(6):547-561
Kieffer M.,Master V.,Waites R.,and Davies B.,2011,TCP14and TCP15 affect internode length and leaf shape in Arabidopsis,Plant J.,68(1):147-158
Kosugi S.,and Ohashi Y.,1997,PCF1 and PCF2 specifically bind to cis elements in the rice proliferating cell nuclear antigen gene,Plant Cell,9(9):1607-1619
Koyama T.,Furutani M.,Tasaka M.,and Ohme-Takagi M.,2007,TCP transcription factors control the morphology of shoot lateral organs via ne gative regulation of the expression of bound ary-specific genes in Arabidopsis,Plant Cell,19(2):473-484
Liu H.,Wang T.T.,Zhang J.H.,Ouyang B.,and Li H.X.,2015,Cloning and expression analysis of a NAC transcription factor Sl NAC80 in tomato,Huabei Nongxuebao(Acta Agriculturae Boreali-Sinca),30(1):77-83(刘辉,王涛涛,张俊红,欧阳波,李汉霞,2015,番茄NAC转录因子Sl NAC80的克隆及表达分析,华北农学报,30(1):77-83)
Liu W.N.,Han J.M.,Dong J.G.,and Li X.Y.,2010,Cloning and expression analysis of cotton(Gossypium hirsutum L.)transcription factor gene GhTCP1,Mianhua Xuebao(Cotton Science),22(5):409-414(刘伟娜,韩建民,董金皋,李学勇,2010,棉花TCP家族转录因子基因GhTCP1的克隆与表达分析,棉花学报,22(5):409-414)
Liu X.,Hu Z.L.,Zhang Y.J.,Zhu M.K.,Yin W.C.,and Chen G.P.,2014,Cloning,bioinformatics and expression analysis of SlMYBL gene in tomato,Zhiwu Yanjiu(Bulletin of Botanical Research),34(5):664-670(刘霞,胡宗利,张彦杰,朱明库,殷文成,陈国平,2014,番茄SlMYBL基因的表达分析及功能研究,植物研究,34(5):664-670)
Liu Y.H.,Yu L.,Ding J.H.,Wang R.Z.,Huang Z.G.,and Xiao L.T.,2012,Research progress in synergistic regulatory roles of phytohormones in shoot branching,Zhiwu Shengli Xuebao(Plant Physiology Journal),48(10):941-948(刘拥海,俞乐,丁君辉,王若仲,黄志刚,萧浪涛,2012,植物激素对分枝发育的协同调控作用研究进展,植物生理学报,48(10):941-948)
Lópezjuez E.,Dillon E.,Magyar Z.,Khan S.,Hazeldine S.,De Jager S.M.,Murray J.A.,Beemster G.T.,B觟gre L.,and Shanahan H.,2008,Distinct light-initiated gene expression and cell cycle programs in the shoot apex and cotyledons of Arabidopsis,Plant Cell,20(4):947-968
Martíntrillo M.,and Cubas P.,2010,TCP genes:a family snapshot ten years later,Trends Plant Sci.,15(1):31-39
Martíntrillo M.,Grandío E.G.,Serra F.,Marcel F.,Rodríguez-Buey M.L.,Schmitz G.,Theres K.,Bendahmane A.,Dopazo H.,and Cubas P.,2011,Role of tomato BRANCHED1-like genes in the control of shoot branching,Plant J.,67(4):701-714
Niu Y.L.,Jiang X.M.,and Xu X.Y.,2016,Cloning and VIGSvector construction of GRAS transcription factors family gene SIGLD1 from tomato,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(8):2155-2160(牛义岭,姜秀明,许向阳,2016,番茄GRAS转录因子家族基因SIGLD1的克隆及VIGS载体构建,基因组学与应用生物学,35(8):2155-2160)
Pagnussat G.C.,Yu H.J.,Ngo Q.A.,Rajani S.,Mayalagu S.,Johnson C.S.,Capron A.,Xie L.F.,Ye D.,and Sundaresan V.,2005,Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis,Development,132(3):603-614
Parapunova V.,Busscher M.,Busscherlange J.,Lammers M.,Karlova R.,Bovy A.G.,Angenent G.C.,and De Maagd R.A.,2014,Identification,cloning and characterization of the tomato TCP transcription factor family,BMC Plant Biol.,14(1):157
Pozacarrión C.,Aguilarmartínez J.A.,and Cubas P.,2007,Role of TCP gene BRANCHED1 in the control of shoot branching in Arabidopsis,Plant Signaling Behav.,2(6):551-552
Ruuska S.A.,Girke T.,Benning C.,and Ohlrogge J.B.,2002,Contrapuntal networks of gene expression during Arabidopsis seed filling,Plant Cell,14(6):1191-1206
Takeda T.,Amano K.,Ohto M.A.,Nakamura K.,Sato S.,Kato T.,Tabata S.,and Ueguchi C.,2006,RNA interference of the Arabidopsis putative transcription factor TCP16 gene results in abortion of early pollen development,Plant Mol.Biol.,61(1-2):165-177
Wang S.S.,Chen G.P.,Zhang Y.J.,Yin W.C.,Zhu M.K.,Zhang J.L.,Tang Q.L.,and Hu Z.L.,2015,Cloning and expression analysis of a transcription factor SlYABBY5 in Solanum lycopersicum,Zhiwu Yanjiu(Bulletin of Botanical Research),35(2):240-249(王苏苏,陈国平,张彦杰,殷文成,朱明库,张建苓,唐区林,胡宗利,2015,番茄转录因子SlYABBY5的克隆与表达分析,植物研究,35(2):240-249)
An J.,Guo Z.,Gou X.,and Li J.,2011,TCP1 positively regulates the expression of DWF4 in Arabidopsis thaliana,Plant Signal Behav.,6(8):1117-1118
Bai F.,and Schmidt R.J.,2012,TCP transcription factor,BRANCH ANGLE DEFECTIVE 1(BAD1),is required for normal tassel branch angle formation in maize,Proc.Natl.Acad.Sci.USA,109(30):12225-12230
Braun N.,De S.G.A.,Pillot J.P.,Boutet-Mercey S.,Dalmais M.,Antoniadi I.,Li X.,Maia-Grondard A.,Le S.C.,Bouteiller N.,Luo D.,Bendahmane A.,Turnbull C.,and Rameau C.,2012,The pea TCP transcription factor PsBRC1 acts downstream of strigolactones to control shoot branching,Plant Physiol.,158(1):225-238
Chapman M.A.,Tang S.,Draeger D.,Nambeesan S.,Shaffer H.,Barb J.G.,Knapp S.J.,and Burke J M.,2012,Genetic analysis of floral symmetry in van gogh's sunflowers reveals independent recruitment of CYCLOIDEA genes in the asteraceae,PLoS Genet.,8(3):e1002628
Citerne H.L.,Mller M.,and Cronk Q.C.B.,2000,Diversity of cycloidea-like genes in gesneriaceae in relation to floral symmetry,Anna.Bot.,86(1):167-176
Cubas P.,Vincent C.,and Coen E.,1999,An epigenetic mutation responsible for natural variation in floral symmetry,Nature,401(6749):157-161
Dai C.,Peng Z.S.,Yang Z.J.,Liao M.L.,Lu L.,Wei S.H.,Zhang L.,Luo Q.,and Tang H.F.,2017,Cloning and expression analysis of glucosyltransferase gene TaUGT73D1 in wheat,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(6):2048-2057(代畅,彭正松,杨在君,廖明莉,路璐,魏淑红,张莉,罗琴,唐海峰,2017,小麦糖基转移酶基因TaUGT73D1的克隆及表达分析,分子植物育种,15(6):2048-2057)
Danisman S.,Wal F.V.D.,Dhondt S.,Waites R.,Folter S.D.,Bimbo A.,Van Dijk A.D.J.,Muino J.M.,Cutri L.,Dornelas M.C.,Angenent G.C.,and Immink R.G.H.,2012,Arabidopsis classⅠand classⅡTCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically,Plant Physiol.,159(4):1511-1523
Doebley J.,Stec A.,and Gustus C.,1995,teosinte branched1 and the origin of maize:evidence for epistasis and the evolution of dominance,Genetics,141(1):333-346
Du L.X.,Hu Z.L.,Zhang J.L.,and Chen G.P.,2014,Cloning,bioinformatics and expression analysis of SlDP1 gene in tomato,Zhiwu Yanjiu(Bulletin of Botanical Research),34(1):68-74(杜利欣,胡宗利,张建苓,陈国平,2014,番茄SlDP1基因的克隆、生物信息学及表达特性分析,植物研究,34(1):68-74)
Finlayson S.A.,2007,Arabidopsis teosinte Branched1-like 1 regulates axillary bud outgrowth and is homologous to monocot Teosinte Branched1,Plant Cell Physiol.,48(5):667-677
Guo Z.X.,Fujioka S.,Blancaflor E.B.,Miao S.,Gou X.P.,and Li J.,2010,TCP1 modulates brassinosteroid biosynthesis by regulating the expression of the key biosynthetic gene DW-ARF4 in Arabidopsis thaliana,Plant Cell,22(4):1161-1173
Ji Z.R.,Gu Y.B.,Dong Q.L.,Chi F.M.,Qiao Z.,Xu C.N.,Zhang J.X.,and Zhou Z.S.,2015,Genome-wide identification and analysis of TCP gene family in grape,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(10):2194-2199(冀志蕊,谷彦冰,董庆龙,迟福梅,乔壮,徐成楠,张俊祥,周宗山,2015,葡萄TCP基因家族全基因组鉴定和分析,基因组学与应用生物学,35(10):2194-2199)
Jibran R.,Hunter D.A.,and Dijkwel P.P.,2013,Hormonal regulation of leaf senescence through integration of developmental and stress signals,Plant Mol.Biol.,82(6):547-561
Kieffer M.,Master V.,Waites R.,and Davies B.,2011,TCP14and TCP15 affect internode length and leaf shape in Arabidopsis,Plant J.,68(1):147-158
Kosugi S.,and Ohashi Y.,1997,PCF1 and PCF2 specifically bind to cis elements in the rice proliferating cell nuclear antigen gene,Plant Cell,9(9):1607-1619
Koyama T.,Furutani M.,Tasaka M.,and Ohme-Takagi M.,2007,TCP transcription factors control the morphology of shoot lateral organs via ne gative regulation of the expression of bound ary-specific genes in Arabidopsis,Plant Cell,19(2):473-484
Liu H.,Wang T.T.,Zhang J.H.,Ouyang B.,and Li H.X.,2015,Cloning and expression analysis of a NAC transcription factor Sl NAC80 in tomato,Huabei Nongxuebao(Acta Agriculturae Boreali-Sinca),30(1):77-83(刘辉,王涛涛,张俊红,欧阳波,李汉霞,2015,番茄NAC转录因子Sl NAC80的克隆及表达分析,华北农学报,30(1):77-83)
Liu W.N.,Han J.M.,Dong J.G.,and Li X.Y.,2010,Cloning and expression analysis of cotton(Gossypium hirsutum L.)transcription factor gene GhTCP1,Mianhua Xuebao(Cotton Science),22(5):409-414(刘伟娜,韩建民,董金皋,李学勇,2010,棉花TCP家族转录因子基因GhTCP1的克隆与表达分析,棉花学报,22(5):409-414)
Liu X.,Hu Z.L.,Zhang Y.J.,Zhu M.K.,Yin W.C.,and Chen G.P.,2014,Cloning,bioinformatics and expression analysis of SlMYBL gene in tomato,Zhiwu Yanjiu(Bulletin of Botanical Research),34(5):664-670(刘霞,胡宗利,张彦杰,朱明库,殷文成,陈国平,2014,番茄SlMYBL基因的表达分析及功能研究,植物研究,34(5):664-670)
Liu Y.H.,Yu L.,Ding J.H.,Wang R.Z.,Huang Z.G.,and Xiao L.T.,2012,Research progress in synergistic regulatory roles of phytohormones in shoot branching,Zhiwu Shengli Xuebao(Plant Physiology Journal),48(10):941-948(刘拥海,俞乐,丁君辉,王若仲,黄志刚,萧浪涛,2012,植物激素对分枝发育的协同调控作用研究进展,植物生理学报,48(10):941-948)
Lópezjuez E.,Dillon E.,Magyar Z.,Khan S.,Hazeldine S.,De Jager S.M.,Murray J.A.,Beemster G.T.,B觟gre L.,and Shanahan H.,2008,Distinct light-initiated gene expression and cell cycle programs in the shoot apex and cotyledons of Arabidopsis,Plant Cell,20(4):947-968
Martíntrillo M.,and Cubas P.,2010,TCP genes:a family snapshot ten years later,Trends Plant Sci.,15(1):31-39
Martíntrillo M.,Grandío E.G.,Serra F.,Marcel F.,Rodríguez-Buey M.L.,Schmitz G.,Theres K.,Bendahmane A.,Dopazo H.,and Cubas P.,2011,Role of tomato BRANCHED1-like genes in the control of shoot branching,Plant J.,67(4):701-714
Niu Y.L.,Jiang X.M.,and Xu X.Y.,2016,Cloning and VIGSvector construction of GRAS transcription factors family gene SIGLD1 from tomato,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(8):2155-2160(牛义岭,姜秀明,许向阳,2016,番茄GRAS转录因子家族基因SIGLD1的克隆及VIGS载体构建,基因组学与应用生物学,35(8):2155-2160)
Pagnussat G.C.,Yu H.J.,Ngo Q.A.,Rajani S.,Mayalagu S.,Johnson C.S.,Capron A.,Xie L.F.,Ye D.,and Sundaresan V.,2005,Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis,Development,132(3):603-614
Parapunova V.,Busscher M.,Busscherlange J.,Lammers M.,Karlova R.,Bovy A.G.,Angenent G.C.,and De Maagd R.A.,2014,Identification,cloning and characterization of the tomato TCP transcription factor family,BMC Plant Biol.,14(1):157
Pozacarrión C.,Aguilarmartínez J.A.,and Cubas P.,2007,Role of TCP gene BRANCHED1 in the control of shoot branching in Arabidopsis,Plant Signaling Behav.,2(6):551-552
Ruuska S.A.,Girke T.,Benning C.,and Ohlrogge J.B.,2002,Contrapuntal networks of gene expression during Arabidopsis seed filling,Plant Cell,14(6):1191-1206
Takeda T.,Amano K.,Ohto M.A.,Nakamura K.,Sato S.,Kato T.,Tabata S.,and Ueguchi C.,2006,RNA interference of the Arabidopsis putative transcription factor TCP16 gene results in abortion of early pollen development,Plant Mol.Biol.,61(1-2):165-177
Wang S.S.,Chen G.P.,Zhang Y.J.,Yin W.C.,Zhu M.K.,Zhang J.L.,Tang Q.L.,and Hu Z.L.,2015,Cloning and expression analysis of a transcription factor SlYABBY5 in Solanum lycopersicum,Zhiwu Yanjiu(Bulletin of Botanical Research),35(2):240-249(王苏苏,陈国平,张彦杰,殷文成,朱明库,张建苓,唐区林,胡宗利,2015,番茄转录因子SlYABBY5的克隆与表达分析,植物研究,35(2):240-249)
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