水产品中嗜水气单胞菌耐药性研究进展
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摘要
嗜水气单胞菌Aeromonas hydrophila具有广泛的致病性,是水生动物最常见的致病菌之一。由于抗生素不合理使用、质粒以及耐药基因的水平转移等因素,来自零售市场、超市和餐厅的即食海鲜产品中,均可分离出大量的嗜水气单胞菌耐药菌株及其耐药基因。因此,探明关键控制点、寻求有效缓解抗生素耐药性的防控策略至关重要。文中介绍了我国嗜水气单胞菌的耐药现状,嗜水气单胞菌的主要侵染及耐药机制,以及目前削减和防控耐药性的主要手段和策略,并对水产品耐药性研究方向和重点作出展望。
As one of the most common pathogens in aquatic animals, Aeromonas hydrophila exhibits a wide range of pathogenicity. Due to factors like unreasonable use of antibiotics and horizontal gene transfer mediated by plasmids, many resistant strains of Aeromonas hydrophila were isolated from ready-to-eat seafood products in retail markets, supermarkets and restaurants. These strains carry many resistance genes. Therefore, it is essential to explore the key control points, and seek for prevention and control strategies so as to effectively alleviate antibiotic resistance. We review here the prevalence of drug resistance of Aeromonas hydrophila in China, and its main infection and resistance mechanisms, and the main means and strategies for reducing and preventing drug resistance. We also address further research directions and focus on drug resistance in Aeromonas hydrophila of the aquatic product.
As one of the most common pathogens in aquatic animals, Aeromonas hydrophila exhibits a wide range of pathogenicity. Due to factors like unreasonable use of antibiotics and horizontal gene transfer mediated by plasmids, many resistant strains of Aeromonas hydrophila were isolated from ready-to-eat seafood products in retail markets, supermarkets and restaurants. These strains carry many resistance genes. Therefore, it is essential to explore the key control points, and seek for prevention and control strategies so as to effectively alleviate antibiotic resistance. We review here the prevalence of drug resistance of Aeromonas hydrophila in China, and its main infection and resistance mechanisms, and the main means and strategies for reducing and preventing drug resistance. We also address further research directions and focus on drug resistance in Aeromonas hydrophila of the aquatic product.
引文
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[6]Jiang X,Qin YX,Lin GF,et al.FlgN plays important roles in the adhesion of Aeromonas hydrophila to host mucus.Genet Mol Res,2015,14(2):6376-6386.
[7]Aronsen GP,Beuerlein MM,Watts DP,et al.Redtail and red colobus monkeys show intersite urinary cortisol concentration variation in Kibale National Park,Uganda.Conserv Physiol,2015,3(1):383-386.
[8]Santos L,Ramos F.Antimicrobial resistance in aquaculture:Current knowledge and alternatives to tackle the problem.Int J Antimicrob Agents,2018,52(2):135-143.
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[11]Ramadan H,Ibrahim N,Samir M,et al.Aeromonas hydrophila from marketed mullet(Mugil cephalus)in Egypt:PCR characterization ofβ-lactam resistance and virulence genes.J Appl Microbiol,2018,124(6):1629-1637.
[12]de Silva BCJ,Hossain S,Dahanayake PS,et al.Aeromonas spp.from marketed Yesso scallop(Patinopecten yessoensis):Molecular characterization,phylogenetic analysis,virulence properties and antimicrobial susceptibility.J Appl Microbiol,2019,126(1):288-299.
[13]Hossain S,de Silva BCJ,Dahanayake PS,et al.Characterization of virulence properties and multi-drug resistance profiles in motile Aeromonas spp.isolated from zebrafish(Danio rerio).Lett Appl Microbiol,2018,67(6):598-605.
[14]Hossain S,de Silva BCJ,Wimalasena SHMP,et al.Distribution of antimicrobial resistance genes and Class1 integron gene cassette arrays in motile Aeromonas spp.isolated from goldfish(Carassius auratus).Microb Drug Resist,2018,24(8):1217-1225.
[15]Zhang M,Yan Q,Mao L,et al.KatG plays an important role in Aeromonas hydrophila survival in fish macrophages and escape for further infection.Gene,2018,672:156-164.
[16]Shi Y,Zhang H,Tian Z,et al.Characteristics of ARG-carrying plasmidome in the cultivable microbial community from wastewater treatment system under high oxytetracycline concentration.Appl Microbiol Biotechnol,2018,102(4):1847-1858.
[17]Fang YF,Pan XY,Lin LY,et al.Molecular mechanisms of quinolone resistance in Aeromonas hydrophilia.Acta Microbiol Sin,2014,54(2):174-182(in Chinese).方一风,潘晓艺,蔺凌云,等.嗜水气单胞菌对喹诺酮类药物耐药的分子机制.微生物学报,2014,54(2):174-182.
[18]Collignon P,Beggs JJ,Walsh TR,et al.Anthropological and socioeconomic factors contributing to global antimicrobial resistance:a univariate and multivariable analysis.Lancet Planet Health,2018,2(9):e398-e405.
[19]Fu J,Yang D,Jin M,et al.Aquatic animals promote antibiotic resistance gene dissemination in water via conjugation:role of different regions within the zebra fish intestinal tract,and impact on fish intestinal microbiota.Mol Ecol,2017,26(19):5318-5333.
[20]Alcaide E,Blasco MD,Esteve C.Mechanisms of quinolone resistance in Aeromonas species isolated from humans,water and eels.Res Microbiol,2010,161(1):40-45.
[21]Mathieu H,Séverine G,Michel F,et al.Role of the AheABC efflux pump in Aeromonas hydrophila intrinsic multidrug resistance.Antimicrob Agents Chemother,2008,52(4):1559-1563.
[22]Chenia HY,Jacobs A.Antimicrobial resistance,heavy metal resistance and integron content in bacteria isolated from a South African tilapia aquaculture system.Dis Aquat Organ,2017,126(3):199-209.
[23]Pu Y,Zhao Z,Li Y,et al.Enhanced efflux activity facilitates drug tolerance in dormant bacterial cells.Mol Cell,2016,62(2):284-294.
[24]Dwyer DJ,Belenky PA,Yang JH,et al.Antibiotics induce redox-related physiological alterations as part of their lethality.Proc Natl Acad Sci USA,2014,111(20):2100-2109.
[25]Lobritz MA,Peter B,Porter CBM,et al.Antibiotic efficacy is linked to bacterial cellular respiration.Proc Natl Acad Sci USA,2015,112(27):8173-8180.
[26]Miller SI.Antibiotic resistance and regulation of the gram-negative bacterial outer membrane barrier by host innate immune molecules.Mbio,2016,7(5):e01541-16.
[27]Heijden JVD,Reynolds LA,Deng W,et al.Salmonella rapidly regulates membrane permeability to survive oxidative stress.Mbio,2016,7(4):e1216-e1238.
[28]Vila-Costa M,Gioia R,Ace?a J,et al.Degradation of sulfonamides as a microbial resistance mechanism.Water Res,2017,115:309-317.
[29]Giri SS,Sukumaran V,Oviya M.Potential probiotic Lactobacillus plantarum VSG3 improves the growth,immunity,and disease resistance of tropical freshwater fish,Labeo rohita.Fish Shellfish Immunol,2013,34(2):660-666.
[30]Lefurgy ST,Malashkevich VN,Aguilan JT,et al.Analysis of the structure and function of FOX-4Cephamycinase.Antimicrob Agents Chemother,2016,60(2):717-728.
[31]Jia X,Zhang J,Sun W,et al.Riboswitch control of aminoglycoside antibiotic resistance.Cell,2013,152(1/2):68-81.
[32]Yi Y,Zhang Z,Zhao F,et al.Probiotic potential of Bacillus velezensis JW:Antimicrobial activity against fish pathogenic bacteria and immune enhancement effects on Carassius auratus.Fish Shellfish Immunol,2018,78:322-330.
[33]Bustillo ME,Fischer AL,Labouyer MA,et al.Modular analysis of hipposin,a histone-derived antimicrobial peptide consisting of membrane translocating and membrane permeabilizing fragments.Biochim Biophys Acta,2014,1838(9):2228-2233.
[34]Le TS,Nguyen TH,Vo HP,et al.Protective effects of bacteriophages against Aeromonas hydrophila species causing motile aeromonas septicemia(MAS)in striped catfish.Antibiotics,2018,7(1):16.
[35]Dong J,Ding H,Liu Y,et al.Magnolol protects channel catfish from Aeromonas hydrophila infection via inhibiting the expression of aerolysin.Vet Microbiol,2017,211:119-123.
[36]Syahidah A,Saad CR,Hassan MD,et al.Phytochemical analysis,identification and quantification of antibacterial active compounds in betel leaves,piper betle methanolic extract.Pak J Biol Sci,2017,20(2):70-81.
[37]Jung-Schroers V,Adamek M,Jung A,et al.Feeding ofβ-1,3/1,6-glucan increases the diversity of the intestinal microflora of carp(Cyprinus carpio).Aquacult Nutr,2016,22(5):1026-1039.
[38]Yildirim-Aksoy M,Beck BH.Antimicrobial activity of chitosan and a chitosan oligomer against bacterial pathogens of warmwater fish.J Appl Microbiol,2017,122(6):1570-1578.
[39]Lejla I,Sommer MOA.Use of collateral sensitivity networks to design drug cycling protocols that avoid resistance development.Sci Transl Med,2013,5(204):132r-204r.
[40]Lázár V,Pal SG,Spohn R,et al.Bacterial evolution of antibiotic hypersensitivity.Mol Syst Biol,2013,9(1):700.
[41]Suzuki S,Horinouchi T,Furusawa C.Suppression of antibiotic resistance acquisition by combined use of antibiotics.J Biosci Bioeng,2015,120(4):467-469.
[42]Suzuki S,Horinouchi T,Furusawa C.Prediction of antibiotic resistance by gene expression profiles.Nat Commun,2014,5(5):5792.
[43]Bandeira JG,Sutili FJ,Gressler LT,et al.Antibacterial potential of phytochemicals alone or in combination with antimicrobials against fish pathogenic bacteria.JAppl Microbiol,2018,125(3):655-665.
[44]Lu J,Wang ZN,Chen R,et al.In vitro antibacterial activity of several Chinese medicine monomers and antibiotics on Aeromonas hydrophila and Aeromonas sobria.J Aquatic Biol,2013,37(6):1128-1132(in Chinese).卢静,王振宁,陈锐,等.几种中药单体和抗生素对嗜水气单胞菌及温和气单胞菌的体外抑菌活性研究.水生生物学报,2013,37(6):1128-1132.
[45]Borgia VJF,Thatheyus AJ,Murugesan AG,et al.Effects of effluent from electoplating industry on the immune response in the freshwater fish,Cyprinus carpio.Fish Shellfish Immunol,2018,79:86-92.
[46]Ying C,Chang MJ,Hu CH,et al.The effects of marine farm-scale sequentially integrated multi-trophic aquaculture systems on microbial community composition,prevalence of sulfonamide-resistant bacteria and sulfonamide resistance gene sul1.Sci Total Environ,2018,643:681-691.
[2]Yingxue Q,Guifang L,Wenbo C,et al.Flagellar motility contributes to the invasion and survival of Aeromonas hydrophila in Anguilla japonica macrophages.Fish Shellfish Immunol,2014,39(2):273-279.
[3]Qin Y,Lin G,Chen W,et al.Flagellar motility is necessary for Aeromonas hydrophila adhesion.Microb Pathogenesis,2016,98:160-166.
[4]Ghatak S,Blom J,Das S,et al.Pan-genome analysis of Aeromonas hydrophila,Aeromonas veronii and Aeromonas caviae indicates phylogenomic diversity and greater pathogenic potential for Aeromonas hydrophila.Anton Leeuw Int JG,2016,109(7):945-956.
[5]Wei G,Qian H,Zhiyong W,et al.Influence of antimicrobial consumption on Gram-negative bacteria in inpatients receiving antimicrobial resistance therapy from 2008-2013 at a tertiary hospital in Shanghai,China.Am J Infect Control,2015,43(4):358-364.
[6]Jiang X,Qin YX,Lin GF,et al.FlgN plays important roles in the adhesion of Aeromonas hydrophila to host mucus.Genet Mol Res,2015,14(2):6376-6386.
[7]Aronsen GP,Beuerlein MM,Watts DP,et al.Redtail and red colobus monkeys show intersite urinary cortisol concentration variation in Kibale National Park,Uganda.Conserv Physiol,2015,3(1):383-386.
[8]Santos L,Ramos F.Antimicrobial resistance in aquaculture:Current knowledge and alternatives to tackle the problem.Int J Antimicrob Agents,2018,52(2):135-143.
[9]Collignon P,Beggs JJ,Walsh TR,et al.Anthropological and socioeconomic factors contributing to global antimicrobial resistance:a univariate and multivariable analysis.Lancet Planet Health,2018,2(9):e398-e405.
[10]Khor WC,Puah SM,Koh TH,et al.Comparison of clinical isolates of Aeromonas from Singapore and Malaysia with regard to molecular identification,virulence,and antimicrobial profiles.Microb Drug Resist,2018,24(4):469-478.
[11]Ramadan H,Ibrahim N,Samir M,et al.Aeromonas hydrophila from marketed mullet(Mugil cephalus)in Egypt:PCR characterization ofβ-lactam resistance and virulence genes.J Appl Microbiol,2018,124(6):1629-1637.
[12]de Silva BCJ,Hossain S,Dahanayake PS,et al.Aeromonas spp.from marketed Yesso scallop(Patinopecten yessoensis):Molecular characterization,phylogenetic analysis,virulence properties and antimicrobial susceptibility.J Appl Microbiol,2019,126(1):288-299.
[13]Hossain S,de Silva BCJ,Dahanayake PS,et al.Characterization of virulence properties and multi-drug resistance profiles in motile Aeromonas spp.isolated from zebrafish(Danio rerio).Lett Appl Microbiol,2018,67(6):598-605.
[14]Hossain S,de Silva BCJ,Wimalasena SHMP,et al.Distribution of antimicrobial resistance genes and Class1 integron gene cassette arrays in motile Aeromonas spp.isolated from goldfish(Carassius auratus).Microb Drug Resist,2018,24(8):1217-1225.
[15]Zhang M,Yan Q,Mao L,et al.KatG plays an important role in Aeromonas hydrophila survival in fish macrophages and escape for further infection.Gene,2018,672:156-164.
[16]Shi Y,Zhang H,Tian Z,et al.Characteristics of ARG-carrying plasmidome in the cultivable microbial community from wastewater treatment system under high oxytetracycline concentration.Appl Microbiol Biotechnol,2018,102(4):1847-1858.
[17]Fang YF,Pan XY,Lin LY,et al.Molecular mechanisms of quinolone resistance in Aeromonas hydrophilia.Acta Microbiol Sin,2014,54(2):174-182(in Chinese).方一风,潘晓艺,蔺凌云,等.嗜水气单胞菌对喹诺酮类药物耐药的分子机制.微生物学报,2014,54(2):174-182.
[18]Collignon P,Beggs JJ,Walsh TR,et al.Anthropological and socioeconomic factors contributing to global antimicrobial resistance:a univariate and multivariable analysis.Lancet Planet Health,2018,2(9):e398-e405.
[19]Fu J,Yang D,Jin M,et al.Aquatic animals promote antibiotic resistance gene dissemination in water via conjugation:role of different regions within the zebra fish intestinal tract,and impact on fish intestinal microbiota.Mol Ecol,2017,26(19):5318-5333.
[20]Alcaide E,Blasco MD,Esteve C.Mechanisms of quinolone resistance in Aeromonas species isolated from humans,water and eels.Res Microbiol,2010,161(1):40-45.
[21]Mathieu H,Séverine G,Michel F,et al.Role of the AheABC efflux pump in Aeromonas hydrophila intrinsic multidrug resistance.Antimicrob Agents Chemother,2008,52(4):1559-1563.
[22]Chenia HY,Jacobs A.Antimicrobial resistance,heavy metal resistance and integron content in bacteria isolated from a South African tilapia aquaculture system.Dis Aquat Organ,2017,126(3):199-209.
[23]Pu Y,Zhao Z,Li Y,et al.Enhanced efflux activity facilitates drug tolerance in dormant bacterial cells.Mol Cell,2016,62(2):284-294.
[24]Dwyer DJ,Belenky PA,Yang JH,et al.Antibiotics induce redox-related physiological alterations as part of their lethality.Proc Natl Acad Sci USA,2014,111(20):2100-2109.
[25]Lobritz MA,Peter B,Porter CBM,et al.Antibiotic efficacy is linked to bacterial cellular respiration.Proc Natl Acad Sci USA,2015,112(27):8173-8180.
[26]Miller SI.Antibiotic resistance and regulation of the gram-negative bacterial outer membrane barrier by host innate immune molecules.Mbio,2016,7(5):e01541-16.
[27]Heijden JVD,Reynolds LA,Deng W,et al.Salmonella rapidly regulates membrane permeability to survive oxidative stress.Mbio,2016,7(4):e1216-e1238.
[28]Vila-Costa M,Gioia R,Ace?a J,et al.Degradation of sulfonamides as a microbial resistance mechanism.Water Res,2017,115:309-317.
[29]Giri SS,Sukumaran V,Oviya M.Potential probiotic Lactobacillus plantarum VSG3 improves the growth,immunity,and disease resistance of tropical freshwater fish,Labeo rohita.Fish Shellfish Immunol,2013,34(2):660-666.
[30]Lefurgy ST,Malashkevich VN,Aguilan JT,et al.Analysis of the structure and function of FOX-4Cephamycinase.Antimicrob Agents Chemother,2016,60(2):717-728.
[31]Jia X,Zhang J,Sun W,et al.Riboswitch control of aminoglycoside antibiotic resistance.Cell,2013,152(1/2):68-81.
[32]Yi Y,Zhang Z,Zhao F,et al.Probiotic potential of Bacillus velezensis JW:Antimicrobial activity against fish pathogenic bacteria and immune enhancement effects on Carassius auratus.Fish Shellfish Immunol,2018,78:322-330.
[33]Bustillo ME,Fischer AL,Labouyer MA,et al.Modular analysis of hipposin,a histone-derived antimicrobial peptide consisting of membrane translocating and membrane permeabilizing fragments.Biochim Biophys Acta,2014,1838(9):2228-2233.
[34]Le TS,Nguyen TH,Vo HP,et al.Protective effects of bacteriophages against Aeromonas hydrophila species causing motile aeromonas septicemia(MAS)in striped catfish.Antibiotics,2018,7(1):16.
[35]Dong J,Ding H,Liu Y,et al.Magnolol protects channel catfish from Aeromonas hydrophila infection via inhibiting the expression of aerolysin.Vet Microbiol,2017,211:119-123.
[36]Syahidah A,Saad CR,Hassan MD,et al.Phytochemical analysis,identification and quantification of antibacterial active compounds in betel leaves,piper betle methanolic extract.Pak J Biol Sci,2017,20(2):70-81.
[37]Jung-Schroers V,Adamek M,Jung A,et al.Feeding ofβ-1,3/1,6-glucan increases the diversity of the intestinal microflora of carp(Cyprinus carpio).Aquacult Nutr,2016,22(5):1026-1039.
[38]Yildirim-Aksoy M,Beck BH.Antimicrobial activity of chitosan and a chitosan oligomer against bacterial pathogens of warmwater fish.J Appl Microbiol,2017,122(6):1570-1578.
[39]Lejla I,Sommer MOA.Use of collateral sensitivity networks to design drug cycling protocols that avoid resistance development.Sci Transl Med,2013,5(204):132r-204r.
[40]Lázár V,Pal SG,Spohn R,et al.Bacterial evolution of antibiotic hypersensitivity.Mol Syst Biol,2013,9(1):700.
[41]Suzuki S,Horinouchi T,Furusawa C.Suppression of antibiotic resistance acquisition by combined use of antibiotics.J Biosci Bioeng,2015,120(4):467-469.
[42]Suzuki S,Horinouchi T,Furusawa C.Prediction of antibiotic resistance by gene expression profiles.Nat Commun,2014,5(5):5792.
[43]Bandeira JG,Sutili FJ,Gressler LT,et al.Antibacterial potential of phytochemicals alone or in combination with antimicrobials against fish pathogenic bacteria.JAppl Microbiol,2018,125(3):655-665.
[44]Lu J,Wang ZN,Chen R,et al.In vitro antibacterial activity of several Chinese medicine monomers and antibiotics on Aeromonas hydrophila and Aeromonas sobria.J Aquatic Biol,2013,37(6):1128-1132(in Chinese).卢静,王振宁,陈锐,等.几种中药单体和抗生素对嗜水气单胞菌及温和气单胞菌的体外抑菌活性研究.水生生物学报,2013,37(6):1128-1132.
[45]Borgia VJF,Thatheyus AJ,Murugesan AG,et al.Effects of effluent from electoplating industry on the immune response in the freshwater fish,Cyprinus carpio.Fish Shellfish Immunol,2018,79:86-92.
[46]Ying C,Chang MJ,Hu CH,et al.The effects of marine farm-scale sequentially integrated multi-trophic aquaculture systems on microbial community composition,prevalence of sulfonamide-resistant bacteria and sulfonamide resistance gene sul1.Sci Total Environ,2018,643:681-691.