中药补肾解毒方对小鼠辐射后机体免疫功能的影响
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摘要
目的观察中药补肾解毒方对小鼠辐射后机体免疫功能的影响。方法 90只无特定病原体级健康雄性小鼠随机分为空白对照组、辐射模型组、氨磷汀组、中药预防组、中药防治结合组,每组18只。除空白对照组外,其他4组小鼠接受一次性60Coγ-射线全身照射,吸收剂量为2 Gy。中药预防组和防治结合组照射前均采用补肾解毒方灌胃,中药防治结合组照射后继续采用补肾解毒方灌胃。均于照射后第1、7、14天取材,每组每次6只,眼球取血检测血清白细胞介素12(IL-12)、γ-干扰素(γ-IFN)含量。结果辐射后第1天,氨磷汀组、中药防治结合组IL-12水平明显低于辐射模型组、中药预防组[(65±16)、(66±34) ng/L比(94±42)、(96±21) ng/L](均P <0. 05)。辐射后第7天,5组间IL-12水平差异均无统计学意义(均P> 0. 05)。辐射后第14天,中药预防组IL-12水平明显低于空白对照组[(67±31) ng/L比(95±38) ng/L],中药防治结合组IL-12水平[(94±15) ng/L]明显高于中药预防组(均P <0. 05)。辐射后第1天,辐射模型组、氨磷汀组、中药预防组、中药防治结合组γ-IFN水平均明显低于空白对照组[(67±13)、(51±8)、(77±14)、(80±13) g/L比(106±32) g/L],中药预防组、中药防治结合组明显高于氨磷汀组(均P <0. 05)。辐射后第7天,空白对照组γ-IFN水平明显低于其他4组[(66±33) g/L比(101±13)、(98±14)、(108±9)、(122±26) g/L](均P <0. 05)。辐射后第14天,5组间γ-IFN水平比较差异均无统计学意义(均P> 0. 05)。结论补肾解毒方防治结合的给药方式较单纯辐射前预防给药效应明显持久,且可使机体IL-12、γ-IFN含量达到甚至略高于健康机体内含量水平,有效、持久、安全地增强了机体自身的免疫调节功能。
Objective To analyze the effect of Chinese herbs tonifying-kidney and detoxification prescription on immunologic function in mice after radiation. Methods Ninety specific pathogen free healthy male mice were randomly divided into blank control group,model control group,amifostine group,pretreatment with traditional Chinese medicine group( TCM prevention group),combination of prevention and cure with traditional Chinese medicine group( TCM combination group),with 18 mice in each group. The mice were treated with 2 Gy60 Coγ-radiation except in blank control group. TCM prevention group and TCM combination group had gastric administration of tonifying-kidney and detoxification prescription before γ-radiation,and TCM combination group continued the intervention after γ-radiation. Eyeball blood was extracted in respectively 6 mice in each group on the 1,7,14 days after radiation to test levels of serum interleukin-12( IL-12) and γ-interferon( γ-IFN). Results On the 1 st day after radiation,IL-12 levels in amifostine group,TCM combination group were significantly lower than those in model control group and TCM prevention group[( 65 ± 16),( 66 ± 34) ng/L vs( 94 ± 42),( 96 ± 21) ng/L]( all P < 0. 05). On the 7 th day,there was no significant difference of IL-12 level among the 5 groups( P > 0. 05). On the 14 th day,IL-12 level in TCM prevention group was significantly lower than that in blank control group[( 67 ±31) ng/L vs( 95 ± 38) ng/L]and it in TCM combination group[( 94 ± 15) ng/L]was significantly higher than that in TCM prevention group( both P < 0. 05). On the 1 st day after radiation,γ-IFN levels in model control group,amifostine group,TCM prevention group and TCM combination group were significantly lower than those in blank control group[( 67 ± 13),( 51 ± 8),( 77 ± 14),( 80 ± 13) g/L vs( 106 ± 32) g/L]; the levels in TCM prevention group and TCM combination group were significantly higher than that in amifostine group( P < 0. 05). On the 7 th day,γ-IFN level in blank model control group was significantly lower than that in the other 4 groups[( 66 ± 33) g/L vs( 101 ± 13),( 98 ± 14),( 108 ± 9),( 122 ± 26) g/L]( P < 0. 05). On the 14 th day,there was no significant difference of γ-IFN level among 5 groups( P > 0. 05). Conclusion Combination of preventive and curative therapy with Chinese medicine tonifying-kidney and detoxification prescription shows an effective and safe regulatory function in protecting the immunity of mice treated with radiation; serum levels of IL-12 and γ-IFN can be persistently improved and recover to normal levels.
Objective To analyze the effect of Chinese herbs tonifying-kidney and detoxification prescription on immunologic function in mice after radiation. Methods Ninety specific pathogen free healthy male mice were randomly divided into blank control group,model control group,amifostine group,pretreatment with traditional Chinese medicine group( TCM prevention group),combination of prevention and cure with traditional Chinese medicine group( TCM combination group),with 18 mice in each group. The mice were treated with 2 Gy60 Coγ-radiation except in blank control group. TCM prevention group and TCM combination group had gastric administration of tonifying-kidney and detoxification prescription before γ-radiation,and TCM combination group continued the intervention after γ-radiation. Eyeball blood was extracted in respectively 6 mice in each group on the 1,7,14 days after radiation to test levels of serum interleukin-12( IL-12) and γ-interferon( γ-IFN). Results On the 1 st day after radiation,IL-12 levels in amifostine group,TCM combination group were significantly lower than those in model control group and TCM prevention group[( 65 ± 16),( 66 ± 34) ng/L vs( 94 ± 42),( 96 ± 21) ng/L]( all P < 0. 05). On the 7 th day,there was no significant difference of IL-12 level among the 5 groups( P > 0. 05). On the 14 th day,IL-12 level in TCM prevention group was significantly lower than that in blank control group[( 67 ±31) ng/L vs( 95 ± 38) ng/L]and it in TCM combination group[( 94 ± 15) ng/L]was significantly higher than that in TCM prevention group( both P < 0. 05). On the 1 st day after radiation,γ-IFN levels in model control group,amifostine group,TCM prevention group and TCM combination group were significantly lower than those in blank control group[( 67 ± 13),( 51 ± 8),( 77 ± 14),( 80 ± 13) g/L vs( 106 ± 32) g/L]; the levels in TCM prevention group and TCM combination group were significantly higher than that in amifostine group( P < 0. 05). On the 7 th day,γ-IFN level in blank model control group was significantly lower than that in the other 4 groups[( 66 ± 33) g/L vs( 101 ± 13),( 98 ± 14),( 108 ± 9),( 122 ± 26) g/L]( P < 0. 05). On the 14 th day,there was no significant difference of γ-IFN level among 5 groups( P > 0. 05). Conclusion Combination of preventive and curative therapy with Chinese medicine tonifying-kidney and detoxification prescription shows an effective and safe regulatory function in protecting the immunity of mice treated with radiation; serum levels of IL-12 and γ-IFN can be persistently improved and recover to normal levels.
引文
[1]李延晖,张蓉,杨云霜,等.补肾解毒方影响辐射小鼠TLR4信号通路下游炎性因子表达的机制研究[J].陕西中医药,2016,37(9):1256-1258. DOI:10. 3969/j. issn. 1000-7369. 2016.09. 071.Li YH,Zhang R,Yang YS,et al. Mechanism of Bushen Jiedu recipe on expression of inflammatory factors in lower reaches of TLR4 signaling pathway in radiation mice[J]. Shaanxi Traditional Chinese Medicine,2016,37(9):1256-1258. DOI:10. 3969/j.issn. 1000-7369. 2016. 09. 071.
[2] Taijima G,Delisle AJ,Hoang K,et al. Immune system phenotyping of radiation and radiation combined injury inoutberd mice[J]. Radiat Res,2013,179(1):101-112. DOI:10. 1667/RR3120. 1.
[3] Riehl TE,Foster L,Stenson WF. Hyaluronic acid is radioprotective in the intestine through a TLR4 and COX-2 mediated mechanism[J].Am J Physiol Gastrointest Liver Physiol,2012,302(3):G309-316. DOI:10. 1152/ajpgi. 00248. 2011.
[4] Tong F,Zhang J,Liu L,et al. Corilagin attenuates radiationinduced brain injury in mice[J]. Mol Neurobiol,2016,53(10):6982-6996. DOI:10. 1007/s12035-015-9591-6.
[5] Li Q,Xu X,Zhong W,et al. IL-17 induces radiation resistance of B lymphoma cells by suppressing p53 expression and thereby inhibiting irradiation-triggered apoptosis[J]. Cell Mol Immunol,2015,12(3):366-372. DOI:10. 1038/cmi. 2014. 122.
[6] Wang H,Sun RT,Li Y,et al. HGF gene modification in mesenchymal stem cells reduces adiation-induced intestinal injury by modulating immunnity[J]. PLo S One,2015,10(5):e0124420.DOI:10. 1371/journal. pone. 0124420.
[7] Ha CT,Li XH,Fu D,et al. Circulating interleukin-18 as a biomarker of total-body radiation exposure in mice,minipigs,and nonhuman primates(NHP)[J]. PLo S One,2014,9(10):e109249. DOI:10. 1371/journal. pone. 0109249.
[8]薛姝婧,刘法东,聂黎虹,等.高温高湿环境对自发性高血压大鼠IL-2、IL-12及淋巴细胞增殖的影响[J].宁夏医科大学学报,2017,39(4):408-411,424. DOI:10. 16050/j. cnki. issn1674-6309. 2017. 04. 010.Xue SJ,Liu FD,Nie LH,et al. Effect of high temperature and humidity environment in spontaneously hypertensive rats on the lymphocyte proliferation and the contents of IL-2 and IL-12[J].Journal of Ningxia Medical University,2017,39(4):408-411,424. DOI:10. 16050/j. cnki. issn1674-6309. 2017. 04. 010.
[9]徐小倩,朱谊,王晓华.六味地黄丸(汤)研究进展[J].哈尔滨医药,2016,36(4):484-486.Xu XQ,Zhu Y,Wang XH. Research progress of Liuwei Dihuang pills(decoction)[J]. Harbin Medicine,2016,36(4):484-486.
[10]翟春涛,田岳凤.六味地黄汤调节机体免疫功能的作用分析[J].世界中西医结合杂志,2017,12(5):606-610. DOI:10.13935/j. cnki. sizx. 170504.Zhai CT,Tian YF. Analysis of Liuwei Dihuang decoction in the regulation of body immune functions[J]. World Journal of Integrated Traditional Chinese and Western Medicine, 2017,12(5):606-610. DOI:10. 13935/j. cnki. sizx. 170504.
[11]严明,闫兆,周林福,等.冬虫夏草药用特点及其临床应用现状[J].首都食品与医药,2017,24(6):68. DOI:10. 3969/j.issn. 1005-8257. 2017. 06. 041.Yan M,Yan Z,Zhou LF,et al. Medicinal characteristics and clinical application of cordyceps[J]. Capital Medicine,2017,24(6):68. DOI:10. 3969/j. issn. 1005-8257. 2017. 06. 041.
[12]王露瑶,梁凌玲,莫小强,等.白花蛇舌草注射液抗肿瘤和免疫调节作用实验分析[J].世界最新医学信息文摘,2017,17(21):1-3. DOI:10. 3969/j. issn. 1671-3141. 2017. 21. 001.Wang LY,Liang LL,Mo XQ,et al. Experimental analysis of antitumor and immunomodulatory effects of hedyotis diffusa injection[J]. World Latest Medicine Information,2017,17(21):1-3. DOI:10. 3969/j. issn. 1671-3141. 2017. 21. 001.
[2] Taijima G,Delisle AJ,Hoang K,et al. Immune system phenotyping of radiation and radiation combined injury inoutberd mice[J]. Radiat Res,2013,179(1):101-112. DOI:10. 1667/RR3120. 1.
[3] Riehl TE,Foster L,Stenson WF. Hyaluronic acid is radioprotective in the intestine through a TLR4 and COX-2 mediated mechanism[J].Am J Physiol Gastrointest Liver Physiol,2012,302(3):G309-316. DOI:10. 1152/ajpgi. 00248. 2011.
[4] Tong F,Zhang J,Liu L,et al. Corilagin attenuates radiationinduced brain injury in mice[J]. Mol Neurobiol,2016,53(10):6982-6996. DOI:10. 1007/s12035-015-9591-6.
[5] Li Q,Xu X,Zhong W,et al. IL-17 induces radiation resistance of B lymphoma cells by suppressing p53 expression and thereby inhibiting irradiation-triggered apoptosis[J]. Cell Mol Immunol,2015,12(3):366-372. DOI:10. 1038/cmi. 2014. 122.
[6] Wang H,Sun RT,Li Y,et al. HGF gene modification in mesenchymal stem cells reduces adiation-induced intestinal injury by modulating immunnity[J]. PLo S One,2015,10(5):e0124420.DOI:10. 1371/journal. pone. 0124420.
[7] Ha CT,Li XH,Fu D,et al. Circulating interleukin-18 as a biomarker of total-body radiation exposure in mice,minipigs,and nonhuman primates(NHP)[J]. PLo S One,2014,9(10):e109249. DOI:10. 1371/journal. pone. 0109249.
[8]薛姝婧,刘法东,聂黎虹,等.高温高湿环境对自发性高血压大鼠IL-2、IL-12及淋巴细胞增殖的影响[J].宁夏医科大学学报,2017,39(4):408-411,424. DOI:10. 16050/j. cnki. issn1674-6309. 2017. 04. 010.Xue SJ,Liu FD,Nie LH,et al. Effect of high temperature and humidity environment in spontaneously hypertensive rats on the lymphocyte proliferation and the contents of IL-2 and IL-12[J].Journal of Ningxia Medical University,2017,39(4):408-411,424. DOI:10. 16050/j. cnki. issn1674-6309. 2017. 04. 010.
[9]徐小倩,朱谊,王晓华.六味地黄丸(汤)研究进展[J].哈尔滨医药,2016,36(4):484-486.Xu XQ,Zhu Y,Wang XH. Research progress of Liuwei Dihuang pills(decoction)[J]. Harbin Medicine,2016,36(4):484-486.
[10]翟春涛,田岳凤.六味地黄汤调节机体免疫功能的作用分析[J].世界中西医结合杂志,2017,12(5):606-610. DOI:10.13935/j. cnki. sizx. 170504.Zhai CT,Tian YF. Analysis of Liuwei Dihuang decoction in the regulation of body immune functions[J]. World Journal of Integrated Traditional Chinese and Western Medicine, 2017,12(5):606-610. DOI:10. 13935/j. cnki. sizx. 170504.
[11]严明,闫兆,周林福,等.冬虫夏草药用特点及其临床应用现状[J].首都食品与医药,2017,24(6):68. DOI:10. 3969/j.issn. 1005-8257. 2017. 06. 041.Yan M,Yan Z,Zhou LF,et al. Medicinal characteristics and clinical application of cordyceps[J]. Capital Medicine,2017,24(6):68. DOI:10. 3969/j. issn. 1005-8257. 2017. 06. 041.
[12]王露瑶,梁凌玲,莫小强,等.白花蛇舌草注射液抗肿瘤和免疫调节作用实验分析[J].世界最新医学信息文摘,2017,17(21):1-3. DOI:10. 3969/j. issn. 1671-3141. 2017. 21. 001.Wang LY,Liang LL,Mo XQ,et al. Experimental analysis of antitumor and immunomodulatory effects of hedyotis diffusa injection[J]. World Latest Medicine Information,2017,17(21):1-3. DOI:10. 3969/j. issn. 1671-3141. 2017. 21. 001.