响应面法优化菌种Acinetobacter gerneri CLX-6降解苯胺印染废水
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摘要
筛选菌种Acinetobacter gerneri CLX-6降解苯胺印染废水,考察培养温度、培养pH、装液量对苯胺降解率的影响,确定单因素培养条件:培养温度30℃、培养pH=7.0、装液量150 mL,苯胺的降解率高达92.19%。然后利用数学软件SAS系统,通过中心响应面分析法中的旋转中心组合设计法优化了苯胺降解条件,最佳培养条件为培养温度33℃、培养pH=6.0,装液量为180 mL,苯胺降解率可达96.7%。菌种CLX-6对印染废水的应用研究表明,将其用于处理苯胺印染废水前景可观。
Through screening strains Acinetobacter gerneri CLX-6 for the degradation of aniline printing and dyeing wastewater, the effects of temperature, pH values and liquid filling quantity on the degradation rate of aniline, the optimum culture conditions are investigated. The condition is that the temperature is 30 ℃, pH=7.0 and liquid filling quantity is 150 mL, which contributes to the highest degradation rate of aniline(92.19%). With the Statistical Aanalysis Ssystem(SAS) software, the aniline degradation conditions have been optimized through one of the Center Response Surface Analysis, namely, rotating center combinatorial design. The optimum condition is that the culture temperature is 33 ℃, pH=6.0 and liquid filling quantity is 180 mL, which exhibits an aniline degradation rate of 96.7%. This study indicates that CLX-6 has a promising application in degrading aniline printing and dyeing wastewater.
Through screening strains Acinetobacter gerneri CLX-6 for the degradation of aniline printing and dyeing wastewater, the effects of temperature, pH values and liquid filling quantity on the degradation rate of aniline, the optimum culture conditions are investigated. The condition is that the temperature is 30 ℃, pH=7.0 and liquid filling quantity is 150 mL, which contributes to the highest degradation rate of aniline(92.19%). With the Statistical Aanalysis Ssystem(SAS) software, the aniline degradation conditions have been optimized through one of the Center Response Surface Analysis, namely, rotating center combinatorial design. The optimum condition is that the culture temperature is 33 ℃, pH=6.0 and liquid filling quantity is 180 mL, which exhibits an aniline degradation rate of 96.7%. This study indicates that CLX-6 has a promising application in degrading aniline printing and dyeing wastewater.
引文
[1]马详麟,付爱萍.苯胺工业废水处理技术新进展[J].化学工程与装备,2012(5):139-141.
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[4]曾国驱,任随周,许玫英,等.微生物降解苯胺的研究现状[J].中国医学生物技术应用杂志,2003,(4):74-78.
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[7]钟文辉,何国庆,郑平,等.2,4-二氯酚降解细菌菌株对含2,4-二氯酚废水的好氧处理[J].浙江大学学报:农业与生命科学版,2003,29(5):550-555.
[8]FUJII T,TAKEO M,MAEDA.Plasmid-encodedgenes specifying aniline oxidation from Acinetobacter sp.strain YAA[J].Microbiology,1997,143:93-99.
[9]蔡莉.固定化高效苯胺降解菌的制备及处理苯胺印染废水的研究[J].印染助剂,2017,34(8):34-38.
[10]曹月坤,林晓东,李长波,等.反硝化细菌固定化降解亚硝酸盐的研究[J].应用化工,2015(10):1 784-1 787.
[2]吴丽丽,周集体,张爱丽,等.膜萃取处理高浓度含苯胺废水[J].化工进展,2007,26(5):715-719.
[3]刘志培,杨惠芳,周培谨.微生物降解苯胺的特性及其降解代谢途径[J].应用与环境生物学报,1999,5(S1):5-9.
[4]曾国驱,任随周,许玫英,等.微生物降解苯胺的研究现状[J].中国医学生物技术应用杂志,2003,(4):74-78.
[5]王治业,祝英,季彬,等.生物强化技术处理苯胺废水难降解有机物的研究[J].环境工程,2014,32:16-18.
[6]EMTIAZI G,SATARII M,MAZAHERION F.The utili zation of aniline,chlorinated aniline,and aniline blue as the only source of nitrogen by fungi in water[J].Water Research,2001,35(5):1 219-1 224.
[7]钟文辉,何国庆,郑平,等.2,4-二氯酚降解细菌菌株对含2,4-二氯酚废水的好氧处理[J].浙江大学学报:农业与生命科学版,2003,29(5):550-555.
[8]FUJII T,TAKEO M,MAEDA.Plasmid-encodedgenes specifying aniline oxidation from Acinetobacter sp.strain YAA[J].Microbiology,1997,143:93-99.
[9]蔡莉.固定化高效苯胺降解菌的制备及处理苯胺印染废水的研究[J].印染助剂,2017,34(8):34-38.
[10]曹月坤,林晓东,李长波,等.反硝化细菌固定化降解亚硝酸盐的研究[J].应用化工,2015(10):1 784-1 787.