响应面分析法优化藏红花口服液配方参数及其抗疲劳效果研究
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摘要
以藏红花提取物为原料,通过调配、高压均质搅拌与热压灭菌,得到藏红花口服液,利用响应面分析法考察不同配方参数对其感官评定的影响,同时以雄性小鼠为研究对象,观察其抗疲劳效果。在单因素试验基础上,利用Design-Expert软件,进行回归分析,确定藏红花口服液的最佳配方为:蔗糖含量2.6%、藏红花提取物含量11.4%、黄原胶含量0.38%、羧甲基纤维素钠含量0.15%,感官评分为95.27,接近预测值。与对照组相比,灌药1个月后的试验组小鼠,负重运动时间明显增长(p<0.05)、运动后血乳酸浓度生成量减少(p<0.05)、肝糖原储存量显著增加(p<0.05),表明其具有较好的抗疲劳效果。
Using Crocus sativus extraction as the main raw materials, the Crocus sativus oral liquid was developed through mixing, high pressure homogenizing stir and sterilization, to observe sensory evaluation effect of different formulas, also investigated anti-fatigue effect used mice as research objective. Response surface methodology with design-expert software was used based on the results of single-factor tests. The different contents of main or auxiliary materials were used as investigation factors and the sensory result was employed as the response value with regression analysis to optimize the best formula was as follows: 2.6% saccharose, 11.4% Crocus sativus extraction, 0.38% xanthan gum, 0.15% CMC-Na. The sensory result was up to 95.27, which was close to the theoretical result. After feed one month, the oral liquid could increase glycogen reserve(p<0.05) and inhibit blood lactic acid rice for mice(p<0.05), then prolong it sport time(p<0.05). Therefore, the Crocus sativus oral liquid has anti-fatigue effect.
Using Crocus sativus extraction as the main raw materials, the Crocus sativus oral liquid was developed through mixing, high pressure homogenizing stir and sterilization, to observe sensory evaluation effect of different formulas, also investigated anti-fatigue effect used mice as research objective. Response surface methodology with design-expert software was used based on the results of single-factor tests. The different contents of main or auxiliary materials were used as investigation factors and the sensory result was employed as the response value with regression analysis to optimize the best formula was as follows: 2.6% saccharose, 11.4% Crocus sativus extraction, 0.38% xanthan gum, 0.15% CMC-Na. The sensory result was up to 95.27, which was close to the theoretical result. After feed one month, the oral liquid could increase glycogen reserve(p<0.05) and inhibit blood lactic acid rice for mice(p<0.05), then prolong it sport time(p<0.05). Therefore, the Crocus sativus oral liquid has anti-fatigue effect.
引文
[1]金顺福.复方玉竹抗疲劳保健食品的研制[D].延吉:延边大学,2008
[2]张娜,李林森.藏红花药理作用研究进展[J].药物评价研究,2013,36(5):394-396
[3]李琳琳.藏红花的研究概述[J].中山大学研究生学刊:自然科学、医学版,2008,29(2):46-52
[4]吴频梅,袁丽红,黄晶,等.利用大孔吸附树脂吸附分离藏红花细胞培养液中藏红花素和藏红花苦素[J].食品科学,2011,32(24):1-6
[5]郭志刚,刘瑞芝,刘雪,等.藏红花的叶鞘培养与藏红花素类物质的合成[J].清华大学学报(自然科学版),1999,39(12):4-7
[6]王保强,杨小平,李永红.藏红花药物高能超声提取的工艺参数优化[J].成都信息工程学院学报,2016,31(4):372-376
[7]李俊玲.红薯茎叶功能性成分分析及其饮料的研制[D].杨凌:西北农林科技大学,2008
[8]张红,李红毅,陈斌.运动型乳饮料的抗疲劳作用[J].解放军预防医学杂志,2009,26(6):407-410
[9]邱春红,丘春东.血乳酸检测的临床应用研究[J].检验医学,2013,28(4):337-341
[11]Bird S P,Tarpenning K M,Marino F E.Effects of liquid carbohydrate/essential amino acid ingestion on acute hormonal response during a single bout of resistance exercise in untrained men[J].Nutrition,2006,22(4):367-375
[10]高珊,童英,熊晓燕,等.蒽酮法与试剂盒法测定糖原含量的比较研究[J].首都公共卫生,2011,5(1):38-40
[12]靳欣欣,田英姿.响应面法优化大叶白麻茎中多糖的提取工艺[J].食品研究与开发,2017,38(17)
[13]殷利娟.即食高能抗疲劳食品的研究[D].天津:天津科技大学,2011
[14]何来英,严卫星,楼密密,等.抗疲劳试验中肝糖原测定的意义探讨[J].中国食品卫生杂志,1998,10(6):3-7
[2]张娜,李林森.藏红花药理作用研究进展[J].药物评价研究,2013,36(5):394-396
[3]李琳琳.藏红花的研究概述[J].中山大学研究生学刊:自然科学、医学版,2008,29(2):46-52
[4]吴频梅,袁丽红,黄晶,等.利用大孔吸附树脂吸附分离藏红花细胞培养液中藏红花素和藏红花苦素[J].食品科学,2011,32(24):1-6
[5]郭志刚,刘瑞芝,刘雪,等.藏红花的叶鞘培养与藏红花素类物质的合成[J].清华大学学报(自然科学版),1999,39(12):4-7
[6]王保强,杨小平,李永红.藏红花药物高能超声提取的工艺参数优化[J].成都信息工程学院学报,2016,31(4):372-376
[7]李俊玲.红薯茎叶功能性成分分析及其饮料的研制[D].杨凌:西北农林科技大学,2008
[8]张红,李红毅,陈斌.运动型乳饮料的抗疲劳作用[J].解放军预防医学杂志,2009,26(6):407-410
[9]邱春红,丘春东.血乳酸检测的临床应用研究[J].检验医学,2013,28(4):337-341
[11]Bird S P,Tarpenning K M,Marino F E.Effects of liquid carbohydrate/essential amino acid ingestion on acute hormonal response during a single bout of resistance exercise in untrained men[J].Nutrition,2006,22(4):367-375
[10]高珊,童英,熊晓燕,等.蒽酮法与试剂盒法测定糖原含量的比较研究[J].首都公共卫生,2011,5(1):38-40
[12]靳欣欣,田英姿.响应面法优化大叶白麻茎中多糖的提取工艺[J].食品研究与开发,2017,38(17)
[13]殷利娟.即食高能抗疲劳食品的研究[D].天津:天津科技大学,2011
[14]何来英,严卫星,楼密密,等.抗疲劳试验中肝糖原测定的意义探讨[J].中国食品卫生杂志,1998,10(6):3-7