云南蔓生型普通菜豆资源形态学遗传多样性分析
|
摘要
文章对129份云南地方蔓生型普通菜豆种质资源进行了农艺性状的遗传多样性分析。结果表明,在云南地区的普通菜豆种质资源在形态学上具有丰富的多样性。10个农艺性状的平均变异系数为0.24,其中以单株荚数的变异度最大,达到0.61。平均多样性指数为2.04,各性状间数值差异不大。通过因子分析中的主成分法将10个变量降维获得4个公共因子,含义清晰且解释度较好。利用公共因子得分进行聚类分析,129份菜豆资源可分为2大类5个亚群。其特征是:亚群Ⅰ表现为分枝及结荚多,单荚粒数多,百粒重较小而单株产量高;亚群Ⅱ表现晚熟,荚小,百粒重和单株产量低;亚群Ⅲ表现长荚;亚群Ⅳ资源数量最多,表现分枝少,较早熟;亚群Ⅴ表现为早熟,结荚少,荚型短宽且单荚粒数少,百粒重大。
129 accessions were analyzed for genetic diversity,and 10 morphology characteristics were analyzed. The results showed that the germplasm resources of common bean in Yunnan area had rich diversity in morphology. The results of analysis included Coefficient of Variation(c. v.),Diversity Index(DI),Principal Composition(PC) and Classification. As the result,the average c. v. of 10 morphology characteristics was 0. 24,the c. v. of the Pods per Plant(PP) was 0. 61,which was obvious c. v. value in all morphology characteristics. The DI value didn't appear obviously different on all 10,the average DI was 2. 04. Using the results in PC to do classification analysis,129 materials were divided into 5 groups,and the group1 showed higher PP,smaller seeds but high Single Plant(SP) yield; The group 2 showed late maturity,smaller pods and lower SP yield; The group 3 showed long pod; The group 4 had the most of germplasms,which showed less branches but early maturity; The group 5 showed early maturity,less PP,but higher Hundred Seeds Weight(HSW).
129 accessions were analyzed for genetic diversity,and 10 morphology characteristics were analyzed. The results showed that the germplasm resources of common bean in Yunnan area had rich diversity in morphology. The results of analysis included Coefficient of Variation(c. v.),Diversity Index(DI),Principal Composition(PC) and Classification. As the result,the average c. v. of 10 morphology characteristics was 0. 24,the c. v. of the Pods per Plant(PP) was 0. 61,which was obvious c. v. value in all morphology characteristics. The DI value didn't appear obviously different on all 10,the average DI was 2. 04. Using the results in PC to do classification analysis,129 materials were divided into 5 groups,and the group1 showed higher PP,smaller seeds but high Single Plant(SP) yield; The group 2 showed late maturity,smaller pods and lower SP yield; The group 3 showed long pod; The group 4 had the most of germplasms,which showed less branches but early maturity; The group 5 showed early maturity,less PP,but higher Hundred Seeds Weight(HSW).
引文
[1]Blair M W,Giraldo M C,Buendia H F,et al.Microsatellite marker diversity in common bean(Phaseolus vulgaris L.)[J].Theor Appl Genet,2006,113:100-109.
[2]Kwak M,Gepts P.Structure of genetic diversity in the two major gene pools of common bean(Phaseolus vulgaris L.,Fabaceae)[J].Theor Appl Genet,2009,118:979-992.
[3]Blair M W,González L F,Kimani P M,et al.Genetic diversity,inter-gene pool introgression and nutritional quality of common beans(Phaseolus vulgaris L.)from Central Africa[J].Theor Appl Genet,2010,121:237-248.
[4]Zhang X,Blair M W,Wang S.Genetic diversity of Chinese common bean(Phaseolus vulgaris L.)landraces assessed with simple sequence repeat markers[J].Theor Appl Genet,2008,117:629-640.
[5]Bitocchi E,Bellucci E,Giardini A,et al.Molecular analysis of the parallel domestication of the common bean(Phaseolus vulgaris L.)in Mesoamerica and the Andes[J].New Phytol,2013,197:300-313.
[6]De Ron A M,Papa R,Bitocchi E,et al.Common Bean[M].Grain Legumes,Springer New York,2015:1-36.
[7]Pickersgill B,Debouck D G.Domestication patterns in common bean(Phaseolus vulgaris L.)and the origin of the Mesoamerican and Andean cultivated races[J].Theor Appl Genet,2005,110:432-444.
[8]张晓艳.中国普通菜豆种质资源基因源分析与遗传多样性研究[D].北京:中国农业科学院,2007.
[9]程须珍,王述民.中国食用豆类品种志[M].北京:中国农业科学技术出版社,2009:269-270.
[10]张赤红.普通菜豆种质资源遗传多样性与分类研究[D].北京:中国农业科学院硕士学位论文,2004.
[11]Meza N,Rosas J C,Martín J P,et al.Biodiversity of common bean(Phaseolus vulgaris L.)in Honduras,evidenced by morphological characterization[J].Genetic Resources and Crop Evolution,2013,60:1329-1336.
[12]王述民,张亚芝,魏淑红,等.普通菜豆种质资源描述规范和数据标准[M].北京:中国农业出版社,2006:9-26.
[13]Thompson M D,Brick M A,Mc Ginley J N,et al.Chemical composition and mammary cancer inhibitory activity of dry bean[J].Crop Sci,2009,49:179-186.
[14]王述民,张亚芝,王石宝,等.普通菜豆优异种质联合鉴定与评价[J].作物品种资源,1996(2):5-7.
[15]朱吉风,武晶,王兰芬,等.菜豆种质资源抗普通细菌性疫病鉴定[J].植物遗传资源学报,2015,16(3):467-471.
[16]李龙,王兰芬,武晶,等.普通菜豆品种苗期抗旱性鉴定[J].作物学报,2015,41(6):963-971.
[17]栾非时,崔成焕,王金陵.菜豆种质资源形态标记的研究[J].东北农业大学学报,2001,32:134-138.
[18]Yang Y,Tian K,Hao J,et al.Biodiversity and biodiversity conservation in Yunnan,China[J].Biodiversity&Conservation,2004,13:813-826.
[2]Kwak M,Gepts P.Structure of genetic diversity in the two major gene pools of common bean(Phaseolus vulgaris L.,Fabaceae)[J].Theor Appl Genet,2009,118:979-992.
[3]Blair M W,González L F,Kimani P M,et al.Genetic diversity,inter-gene pool introgression and nutritional quality of common beans(Phaseolus vulgaris L.)from Central Africa[J].Theor Appl Genet,2010,121:237-248.
[4]Zhang X,Blair M W,Wang S.Genetic diversity of Chinese common bean(Phaseolus vulgaris L.)landraces assessed with simple sequence repeat markers[J].Theor Appl Genet,2008,117:629-640.
[5]Bitocchi E,Bellucci E,Giardini A,et al.Molecular analysis of the parallel domestication of the common bean(Phaseolus vulgaris L.)in Mesoamerica and the Andes[J].New Phytol,2013,197:300-313.
[6]De Ron A M,Papa R,Bitocchi E,et al.Common Bean[M].Grain Legumes,Springer New York,2015:1-36.
[7]Pickersgill B,Debouck D G.Domestication patterns in common bean(Phaseolus vulgaris L.)and the origin of the Mesoamerican and Andean cultivated races[J].Theor Appl Genet,2005,110:432-444.
[8]张晓艳.中国普通菜豆种质资源基因源分析与遗传多样性研究[D].北京:中国农业科学院,2007.
[9]程须珍,王述民.中国食用豆类品种志[M].北京:中国农业科学技术出版社,2009:269-270.
[10]张赤红.普通菜豆种质资源遗传多样性与分类研究[D].北京:中国农业科学院硕士学位论文,2004.
[11]Meza N,Rosas J C,Martín J P,et al.Biodiversity of common bean(Phaseolus vulgaris L.)in Honduras,evidenced by morphological characterization[J].Genetic Resources and Crop Evolution,2013,60:1329-1336.
[12]王述民,张亚芝,魏淑红,等.普通菜豆种质资源描述规范和数据标准[M].北京:中国农业出版社,2006:9-26.
[13]Thompson M D,Brick M A,Mc Ginley J N,et al.Chemical composition and mammary cancer inhibitory activity of dry bean[J].Crop Sci,2009,49:179-186.
[14]王述民,张亚芝,王石宝,等.普通菜豆优异种质联合鉴定与评价[J].作物品种资源,1996(2):5-7.
[15]朱吉风,武晶,王兰芬,等.菜豆种质资源抗普通细菌性疫病鉴定[J].植物遗传资源学报,2015,16(3):467-471.
[16]李龙,王兰芬,武晶,等.普通菜豆品种苗期抗旱性鉴定[J].作物学报,2015,41(6):963-971.
[17]栾非时,崔成焕,王金陵.菜豆种质资源形态标记的研究[J].东北农业大学学报,2001,32:134-138.
[18]Yang Y,Tian K,Hao J,et al.Biodiversity and biodiversity conservation in Yunnan,China[J].Biodiversity&Conservation,2004,13:813-826.