基于UPLC-Q-Exactive和HPLC对蒲公英总黄酮部位成分分析及主成分的含量测定
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摘要
目的对蒲公英总黄酮部位的有效物质进行分析及部分成分的含量测定。方法采用四级杆-静电场轨道阱超高分辨质谱仪(UPLC-Q-Exactive)在负离子模式下对有效物质进行分析。采用高效液相色谱法(HPLC)对部分成分进行含量的测定。结果蒲公英总黄酮部位共鉴定出14个化合物,其中3个酚酸类,11个黄酮类成分。建立了咖啡酸、绿原酸、槲皮素、木犀草素和山奈酚的回归方程,线性关系均良好(r>0.999 8),并检测了5种成分的含量。结论 UPLC-Q-Exactive技术与HPLC技术可用于蒲公英总黄酮部位的有效物质定性与定量分析,是一种快速、准确、有效、可靠的方法。
Objective To analyze the effective substances in the total flavonoids of dandelion and determine thecontents of some components. Methods UPLC-Q-Exactive at negative ion mode was used to analyze the activesubstances. The contents of some components were determined by HPLC. Results A total of 14 compounds wereidentified from the total flavonoids of dandelion, including 3 phenolic acids and 11 flavonoids. The regressionequations of caffeic acid, chlorogenic acid, quercetin, luteolin and kaempferol were established. The linearrelationships were good(r > 0.999 8) and the contents of five components were determined. Conclusion UPLC-Q-Exactive technology and HPLC technology can be used for qualitative and quantitative analysis of effective flavonoidsin dandelion. It is a fast,accurate,effective and reliable method.
Objective To analyze the effective substances in the total flavonoids of dandelion and determine thecontents of some components. Methods UPLC-Q-Exactive at negative ion mode was used to analyze the activesubstances. The contents of some components were determined by HPLC. Results A total of 14 compounds wereidentified from the total flavonoids of dandelion, including 3 phenolic acids and 11 flavonoids. The regressionequations of caffeic acid, chlorogenic acid, quercetin, luteolin and kaempferol were established. The linearrelationships were good(r > 0.999 8) and the contents of five components were determined. Conclusion UPLC-Q-Exactive technology and HPLC technology can be used for qualitative and quantitative analysis of effective flavonoidsin dandelion. It is a fast,accurate,effective and reliable method.
引文
[1]国家药典委员会.中华人民共和国药典(2015年版):一部[M].北京:中国医药科技出版社,2015:352.
[2]肖玫,杨进,曹玉华.蒲公英的营养价值及其开发利用[J].中国食物与营养,2005(4):47-48.
[3]杜伟锋,贾永强,姜东京,等.基于HPLC-ESI-MS法续断发汗前后的成分分析[J].中草药,2014,45(22):3251-3255.
[4]张令文,计红芳,孙科祥,等.蒲公英总黄酮的提取工艺及定性分析[J].中国食品添加剂,2010(5):226-230.
[5]李明珠,宋平顺,赵建邦.HPLC测定烈香杜鹃中6种黄酮成分的含量及主成分分析[J].中国实验方剂学杂志,2015,21(8):81-84.
[6]邵军,陈伟康,马双成,等.UPLC法同时测定裸花紫珠中5种类黄酮类成分[J].中草药,2014,45(10):1473-1476.
[7]许兰波,张峰,丁艳霞,等.HPLC法同时测定杜仲叶中6个黄酮类成分的含量[J].药物分析杂志,2014,26(8):1422-1425.
[8]凌云,张雅林,蔡少青,等.蒲公英黄酮类和甾醇类成分的研究[J].中国药物化学杂志,1998,8(1):46-48.
[9]赵金娟,管仁伟,路俊仙,等.HPLC测定不同品种金银花及叶中木犀草苷含量[J].中国实验方剂学杂志,2014,20(6):103-106.
[10]夏伯候,周亚敏,皮胜玲,等.UPLC测定桑叶中抗氧化活性成分异槲皮苷、芦丁和紫云英苷的含量[J].中药材,2016,39(3):586-589.
[11]杜蕾蕾,陈雏.不同沙棘叶中槲皮素、山柰酚和异鼠李素的含量比较[J].成都医学院学报,2015,10(1):28-30,34.
[12]李元元,江振作,张蕾,等.UPLC-Q-TOF/MS法鉴定大鼠体内甘草素的代谢产物[J].天津中医药,2015,32(12):757-762.
[13]林彤,段金廒,钱大玮,等.HPLC-MS/MS联用技术分析鉴定苏薄荷中的黄酮类成分[J].中国天然药物,2006,4(2):111-115.
[14]许先猛,董文宾,卢军,等.蒲公英的化学成分和功能特性的研究进展[J].食品安全质量检测学报,2018,9(7):1623-1627.
[15]赵惠茹,郭婷.反相高效液相色谱法同时测定蒲公英中槲皮素和木犀草素的含量[J].中国药师,2014,17(7):1106-1108.
[2]肖玫,杨进,曹玉华.蒲公英的营养价值及其开发利用[J].中国食物与营养,2005(4):47-48.
[3]杜伟锋,贾永强,姜东京,等.基于HPLC-ESI-MS法续断发汗前后的成分分析[J].中草药,2014,45(22):3251-3255.
[4]张令文,计红芳,孙科祥,等.蒲公英总黄酮的提取工艺及定性分析[J].中国食品添加剂,2010(5):226-230.
[5]李明珠,宋平顺,赵建邦.HPLC测定烈香杜鹃中6种黄酮成分的含量及主成分分析[J].中国实验方剂学杂志,2015,21(8):81-84.
[6]邵军,陈伟康,马双成,等.UPLC法同时测定裸花紫珠中5种类黄酮类成分[J].中草药,2014,45(10):1473-1476.
[7]许兰波,张峰,丁艳霞,等.HPLC法同时测定杜仲叶中6个黄酮类成分的含量[J].药物分析杂志,2014,26(8):1422-1425.
[8]凌云,张雅林,蔡少青,等.蒲公英黄酮类和甾醇类成分的研究[J].中国药物化学杂志,1998,8(1):46-48.
[9]赵金娟,管仁伟,路俊仙,等.HPLC测定不同品种金银花及叶中木犀草苷含量[J].中国实验方剂学杂志,2014,20(6):103-106.
[10]夏伯候,周亚敏,皮胜玲,等.UPLC测定桑叶中抗氧化活性成分异槲皮苷、芦丁和紫云英苷的含量[J].中药材,2016,39(3):586-589.
[11]杜蕾蕾,陈雏.不同沙棘叶中槲皮素、山柰酚和异鼠李素的含量比较[J].成都医学院学报,2015,10(1):28-30,34.
[12]李元元,江振作,张蕾,等.UPLC-Q-TOF/MS法鉴定大鼠体内甘草素的代谢产物[J].天津中医药,2015,32(12):757-762.
[13]林彤,段金廒,钱大玮,等.HPLC-MS/MS联用技术分析鉴定苏薄荷中的黄酮类成分[J].中国天然药物,2006,4(2):111-115.
[14]许先猛,董文宾,卢军,等.蒲公英的化学成分和功能特性的研究进展[J].食品安全质量检测学报,2018,9(7):1623-1627.
[15]赵惠茹,郭婷.反相高效液相色谱法同时测定蒲公英中槲皮素和木犀草素的含量[J].中国药师,2014,17(7):1106-1108.