不同光照处理对柞蚕蛹滞育解除及糖代谢的影响
|
摘要
以自然光处理为对照,采用不同光照时长与光照强度的日光灯照射河南一化性柞蚕品种豫大1号和辽宁二化性柞蚕品种沈黄1号的蚕茧,探讨不同光照处理对柞蚕蛹滞育解除的影响以及柞蚕蛹滞育解除过程中体内海藻糖和糖原的代谢变化。结果表明,400 lx、17 h/d的光照组合对豫大1号的滞育解除效果最好,羽化高峰期时间最早,校正羽化率为80.0%;沈黄1号在260 lx、17 h/d的光照条件下达到最高校正羽化率(89.9%),400 lx、17 h/d为羽化高峰期最早的光照组合。日光灯照射下,豫大1号的平均校正羽化率较沈黄1号低9.1百分点,羽化持续时间长19.16 d,但2种柞蚕蛹都在光照处理50 d左右达到羽化高峰期;自然光照射下,豫大1号的校正羽化率较沈黄1号的羽化率高9.4百分点。无论是在17 h/d日光灯照射还是自然光照射处理期间,豫大1号和沈黄1号滞育蛹的海藻糖含量均无明显的变化规律;豫大1号滞育蛹在自然光照射处理期间糖原含量无明显变化规律,在17 h/d日光灯照射处理期间,糖原含量呈先下降后上升的趋势,而沈黄1号滞育蛹的糖原含量在17 h/d日光灯照射和自然光照射下都呈持续下降的趋势。研究结果将为进一步阐明柞蚕蛹滞育解除过程中的糖类物质变化规律提供理论依据。
In contrast to the natural light,lamps with different illumination time and intensity were used to identify the effect of different light treatments on diapause termination of tussah pupae and the metabolic variations of trehalose and glycogen in the process of diapause termination of tussah pupae. Henan univoltine tussah silkworm variety Yuda 1 and Liaoning bivoltine tussah silkworm variety Shenhuang 1 were chosen as materials. Results showed that the effect of light combination of 400 lx and 17 h/d on diapause termination of Yuda 1 was the best with the earliest peak time of eclosion,and the corrected eclosion rate of 80. 0%. In contrast,Shenhuang 1 reached the highest corrected eclosion rate of 89. 9% under the light combination of 260 lx and 17 h/d,and the earliest peak period of eclosion occurred under the light combination of 400 lx and 17 h/d.Under light treatment,the average corrected eclosion rate of Yuda 1 was 9. 1 percentage points lower than that of Shenhuang 1,and the duration of eclosion of Yuda 1 was19. 16 days longer than that of Shenhuang 1. The tussah pupae of two varieties reached eclosion peak after 50 days of illumination. Under natural light treatment,the corrected eclosion rate of Yuda 1 was 9. 4 percentage points higher than that of Shenhuang 1. There was no significant change in trehalose content of Yuda 1 and Shenhuang 1 under 17 h/d lighting and natural light treatment. There was no significant change in glycogen content of Yuda 1 during natural light treatment,while the glycogen content decreased first and then increased during 17 h/d light treatment. The content of glycogen of Shenhuang 1 all decreased continuously under 17 h/d lighting and natural light treatment. These results will provide a theoretical basis for further elucidation of the changes of carbohydrate content during diapause termination of Antheraea pernyi pupa.
In contrast to the natural light,lamps with different illumination time and intensity were used to identify the effect of different light treatments on diapause termination of tussah pupae and the metabolic variations of trehalose and glycogen in the process of diapause termination of tussah pupae. Henan univoltine tussah silkworm variety Yuda 1 and Liaoning bivoltine tussah silkworm variety Shenhuang 1 were chosen as materials. Results showed that the effect of light combination of 400 lx and 17 h/d on diapause termination of Yuda 1 was the best with the earliest peak time of eclosion,and the corrected eclosion rate of 80. 0%. In contrast,Shenhuang 1 reached the highest corrected eclosion rate of 89. 9% under the light combination of 260 lx and 17 h/d,and the earliest peak period of eclosion occurred under the light combination of 400 lx and 17 h/d.Under light treatment,the average corrected eclosion rate of Yuda 1 was 9. 1 percentage points lower than that of Shenhuang 1,and the duration of eclosion of Yuda 1 was19. 16 days longer than that of Shenhuang 1. The tussah pupae of two varieties reached eclosion peak after 50 days of illumination. Under natural light treatment,the corrected eclosion rate of Yuda 1 was 9. 4 percentage points higher than that of Shenhuang 1. There was no significant change in trehalose content of Yuda 1 and Shenhuang 1 under 17 h/d lighting and natural light treatment. There was no significant change in glycogen content of Yuda 1 during natural light treatment,while the glycogen content decreased first and then increased during 17 h/d light treatment. The content of glycogen of Shenhuang 1 all decreased continuously under 17 h/d lighting and natural light treatment. These results will provide a theoretical basis for further elucidation of the changes of carbohydrate content during diapause termination of Antheraea pernyi pupa.
引文
[1] TAUBER M F,TAUBER C A.Insect seasonality:diapauses maintenance,termination,and postdiapause development[J].Annu Rev Entomol,1976,21:81-107
[2] 任小云,齐晓阳,安涛.滞育昆虫营养物质的积累、转化与调控[J].应用昆虫学报,2016,53(4):685-695
[3] 李爱青,薛芳森.温度和光周期对双色泉蝇及其寄生蜂泉蝇茧蜂滞育维持和解除的影响[J].江西农业大学学报,2002,24(4):436-440
[4] 易传辉,陈晓鸣,史军义,等.温度在柑橘凤蝶蛹滞育解除中的作用[J].应用昆虫学报,2009,46(3):453-456
[5] 王瑜,薛瑞德,董言德.保幼激素与光照对三带喙库蚊滞育发生与解除作用的研究[J].中国媒介生物学及控制杂志,1994,5(6):410-412
[6] 秦利.中国柞蚕学[M].北京:中国科学文化出版社,2003:90-92
[7] 王庆忠.不同光照对一化性柞蚕解除滞育的影响[J].蚕学通讯,2008(1):19-20;24
[8] HAYAKAWA Y,CHINO H.Phosphofructokinase as a possible key enzyme regulating glycerol or trehalose accumulation in diapausing insects[J].Insect Biochem,1982,12(6):639-642
[9] KO?TáL V,SULA J,SIMEK P.Physiology of drought tolerance and cold hardiness of the Mediterranean tiger moth Cymbalophora pudica during summer diapause[J].J Insect Physiol,1998,44(2):165-173
[10] 王荫长.昆虫生物化学[M].北京:中国农业出版社,2001:30-38
[11] STOREY K B,BAUST J G,STOREY J M.Intermediary metabolism during low temperature acclimation in the overwintering gall fly larva,Eurosta solidaginis[J].J Comp Physiol B,1981,144(2):183-190
[12] 陆明贤,杨长松,滕秀银,等.柞蚕蛹脂肪体和血淋巴中糖类含量动态的温度和光周期效应[J].昆虫学报,1992,35(1):1-7
[13] 秦加敏,罗术东,绍禹,等.昆虫海藻糖与海藻糖酶的特性及功能研究[J].环境昆虫学报,2015,37(1):163-169
[14] GOTO M,LI Y,KAYABA S,OUTANI S,et al.Cold hardiness in summer and winter diapause and post-diapause pupae of the cabbage armyworm,Mamestra brassicae L. under temperature acclimation[J].J Insect Physiol,2001,47(7):709-714
[15] 陈鹏,肖琳,史小丽,等.胞内海藻糖在CP02菌降解对苯二甲酸中的作用[J].农业环境科学学报,2004,23(5):977-980
[16] 黄伶,孙良振,王勇,等.柞蚕蛹解除滞育过程中海藻糖合成酶基因的表达变化[J].昆虫学报,2016,59(9):938-947
[17] 杨新峰,郭剑.河南省柞蚕区一化二放秋季制种的比较分析[J].北方蚕业,2015,36(2):36-37
[18] 王坤龙,袁颖.河南一化性柞蚕蛹滞育解除研究初报[J].蚕桑茶叶通讯,2015(4):4-6
[19] THOMPSON S N.Trehalose-the insect “blood” sugar[J].Adv Insect Physiol,2003,31(3):205-285
[2] 任小云,齐晓阳,安涛.滞育昆虫营养物质的积累、转化与调控[J].应用昆虫学报,2016,53(4):685-695
[3] 李爱青,薛芳森.温度和光周期对双色泉蝇及其寄生蜂泉蝇茧蜂滞育维持和解除的影响[J].江西农业大学学报,2002,24(4):436-440
[4] 易传辉,陈晓鸣,史军义,等.温度在柑橘凤蝶蛹滞育解除中的作用[J].应用昆虫学报,2009,46(3):453-456
[5] 王瑜,薛瑞德,董言德.保幼激素与光照对三带喙库蚊滞育发生与解除作用的研究[J].中国媒介生物学及控制杂志,1994,5(6):410-412
[6] 秦利.中国柞蚕学[M].北京:中国科学文化出版社,2003:90-92
[7] 王庆忠.不同光照对一化性柞蚕解除滞育的影响[J].蚕学通讯,2008(1):19-20;24
[8] HAYAKAWA Y,CHINO H.Phosphofructokinase as a possible key enzyme regulating glycerol or trehalose accumulation in diapausing insects[J].Insect Biochem,1982,12(6):639-642
[9] KO?TáL V,SULA J,SIMEK P.Physiology of drought tolerance and cold hardiness of the Mediterranean tiger moth Cymbalophora pudica during summer diapause[J].J Insect Physiol,1998,44(2):165-173
[10] 王荫长.昆虫生物化学[M].北京:中国农业出版社,2001:30-38
[11] STOREY K B,BAUST J G,STOREY J M.Intermediary metabolism during low temperature acclimation in the overwintering gall fly larva,Eurosta solidaginis[J].J Comp Physiol B,1981,144(2):183-190
[12] 陆明贤,杨长松,滕秀银,等.柞蚕蛹脂肪体和血淋巴中糖类含量动态的温度和光周期效应[J].昆虫学报,1992,35(1):1-7
[13] 秦加敏,罗术东,绍禹,等.昆虫海藻糖与海藻糖酶的特性及功能研究[J].环境昆虫学报,2015,37(1):163-169
[14] GOTO M,LI Y,KAYABA S,OUTANI S,et al.Cold hardiness in summer and winter diapause and post-diapause pupae of the cabbage armyworm,Mamestra brassicae L. under temperature acclimation[J].J Insect Physiol,2001,47(7):709-714
[15] 陈鹏,肖琳,史小丽,等.胞内海藻糖在CP02菌降解对苯二甲酸中的作用[J].农业环境科学学报,2004,23(5):977-980
[16] 黄伶,孙良振,王勇,等.柞蚕蛹解除滞育过程中海藻糖合成酶基因的表达变化[J].昆虫学报,2016,59(9):938-947
[17] 杨新峰,郭剑.河南省柞蚕区一化二放秋季制种的比较分析[J].北方蚕业,2015,36(2):36-37
[18] 王坤龙,袁颖.河南一化性柞蚕蛹滞育解除研究初报[J].蚕桑茶叶通讯,2015(4):4-6
[19] THOMPSON S N.Trehalose-the insect “blood” sugar[J].Adv Insect Physiol,2003,31(3):205-285