土荆芥对幽门螺杆菌感染小鼠肠道菌群多样性的影响
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摘要
目的观察幽门螺杆菌(HP)诱导小鼠胃炎模型肠道菌群结构和丰度的变化。获取盲肠内容物进行测序分析,通过分析肠道菌群结构的改变探究长期服用土荆芥提取物对小鼠的影响。方法雄性昆明小鼠30只,随机分为空白组10只、模型组10只,土荆芥组10只。幽门螺杆菌感染成模后给药4周,同一时间取各组小鼠的新鲜直肠粪便。在Illumina HiSeq PE250高通量测序平台上对粪便样本中细菌16S rDNA-V3-V4区进行测序,对肠道菌群结构、丰度进行定量分析。结果在门水平,幽门螺杆菌感染模型组拟杆菌门、变形菌门、疣微菌门、软壁菌门、放线菌门相对丰度降低,厚壁菌门相对丰度升高,与空白组和土荆芥组均有明显差异;在属水平,模型组乳杆菌属、双歧杆菌属相对丰度降低,拟杆菌属、瘤胃球菌属、颤杆菌和理研菌属、肠球菌属、肠单胞菌属等相对丰度升高。土荆芥组肠单胞菌属和理研菌属相对丰度升高,肠球菌属相对丰度降低。结论 HP的定植会显著改变肠道菌群结构,肠道菌群的丰度和多样性降低,HP感染胃炎的发病过程可能与益生菌群减少,有害菌群增加有关。土荆芥干预可以改善HP感染对肠道菌群的影响,增加菌群多样性。
Objective To observe the changes of intestinal flora structure and abundance in mice with gastritis induced by Helicobacter pylori (HP). The cecal contents were obtained for sequencing analysis,and the effects of long-term use of the extract of Chenopodium ambrosioides L. on mice were investigated by analyzing the changes in the structure of the intestinal flora. Methods Thirty male Kunming mice were randomly divided into blank group (n = 10),model group (n = 10) and CAL (Chenopodium ambrosioides L.)group (n = 10). After HP infection was established,they were administered drugs for 4 weeks,and fresh rectal stools of each group of mice were taken at the same time. The 16 S rDNA-V3-V4 region of the fecal samples was sequenced on an Illumina Hi Seq PE250 highthroughput sequencing platform to quantify the structure and abundance of the intestinal flora. Results At the level of the portal,the relative abundance of the Helicobacter pylori infection model group,such as Bacteroides, Proteobacteria, Mycobacterium, Softwalled bacteria,and actinomycetes,were decreased,and the relative abundance of the thick-walled bacteria increased in the model group. There were significant differences with the blank group and the schizonepeta group; at the genus level,the relative abundance of the model group Lactobacillus and Bifidobacterium decreased, Bacteroides, rumen cocci, dystrophic and Phytophthora,Enterococcus,increased. The relative abundance of the genus Monocytogenes was elevated. The relative abundance of Enterobacter and Limerella was increased,and the relative abundance of Enterococcus was decreased. Conclusion This study suggests that colonization of HP significantly could change the structure of the intestinal flora,and the abundance and diversity of the intestinal flora could be reduced. The pathogenesis of HP infection with gastritis may be related to the decrease of probiotics and the increase of harmful flora. Intervention of E. striata can improve the effect of HP infection on intestinal flora and increase the diversity of flora.
Objective To observe the changes of intestinal flora structure and abundance in mice with gastritis induced by Helicobacter pylori (HP). The cecal contents were obtained for sequencing analysis,and the effects of long-term use of the extract of Chenopodium ambrosioides L. on mice were investigated by analyzing the changes in the structure of the intestinal flora. Methods Thirty male Kunming mice were randomly divided into blank group (n = 10),model group (n = 10) and CAL (Chenopodium ambrosioides L.)group (n = 10). After HP infection was established,they were administered drugs for 4 weeks,and fresh rectal stools of each group of mice were taken at the same time. The 16 S rDNA-V3-V4 region of the fecal samples was sequenced on an Illumina Hi Seq PE250 highthroughput sequencing platform to quantify the structure and abundance of the intestinal flora. Results At the level of the portal,the relative abundance of the Helicobacter pylori infection model group,such as Bacteroides, Proteobacteria, Mycobacterium, Softwalled bacteria,and actinomycetes,were decreased,and the relative abundance of the thick-walled bacteria increased in the model group. There were significant differences with the blank group and the schizonepeta group; at the genus level,the relative abundance of the model group Lactobacillus and Bifidobacterium decreased, Bacteroides, rumen cocci, dystrophic and Phytophthora,Enterococcus,increased. The relative abundance of the genus Monocytogenes was elevated. The relative abundance of Enterobacter and Limerella was increased,and the relative abundance of Enterococcus was decreased. Conclusion This study suggests that colonization of HP significantly could change the structure of the intestinal flora,and the abundance and diversity of the intestinal flora could be reduced. The pathogenesis of HP infection with gastritis may be related to the decrease of probiotics and the increase of harmful flora. Intervention of E. striata can improve the effect of HP infection on intestinal flora and increase the diversity of flora.
引文
[1]Shimizu T,Chiba T,Marusawa H.Helicobacter pyloriMediated Genetic Instability and Gastric Carcinogenesis[J].Curr Top Microbiol Immunol,2017,400:305-323.
[2]Alarcon T,Llorca L,Perez-Perez G.Impact of the Microbiota and Gastric Disease Development by Helicobacter pylori[J].Curr Top Microbiol Immunol,2017,400:253-275.
[3]Yang YJ,Sheu BS.Metabolic Interaction of Helicobacter pylori Infection and Gut Microbiota[J].Microorganisms,2016,4(1):247-254.
[4]Liu G,Xie J,Lu ZR,et al.Fifth Chinese national consensus report on the management of Helicobacter pylori infection[J].Zhonghua Nei Ke Za Zhi,2017,56(7):532-545.
[5]Espinoza JL,Matsumoto A,Tanaka H,et al.Gastric microbiota:An emerging player in Helicobacter pyloriinduced gastric malignancies[J].Cancer Lett,2018,414:147-152.
[6]Petra CV,Rus A,Dumitrascu DL.Gastric microbiota:tracing the culprit[J].Clujul Med,2017,90(4):369-376.
[7]叶晖,于靖,张学智.土荆芥挥发油对小鼠体内幽门螺杆菌清除作用及对NF-κB表达的影响[J].中华中医药杂志,2017,32(12):5346-5349.
[8]张月苗,叶晖,王婷婷,等.荆花胃康胶丸联合三联疗法治疗幽门螺杆菌感染的1年随访研究[J].现代中医临床,2015,22(2):8-11.
[9]叶晖,李宁,于靖,等.荆花胃康胶丸对幽门螺杆菌感染小鼠胃黏膜核因子κB p65表达的影响[J].中国中医药信息杂志,2015,22(2):60-63.
[10]史宗明,叶晖,张学智.中成药研究现状的文献可视化分析---以荆花胃康胶丸为例[J].中华中医药学刊,2018,36(3):586-590.
[11]叶晖,李宁,于靖,等.昆明小鼠感染幽门螺杆菌动物模型的建立[J].胃肠病学和肝病学杂志,2015,24(3):284-286.
[12]Jo HJ,Kim J,Kim N,et al.Analysis of Gastric Microbiota by Pyrosequencing:Minor Role of Bacteria Other Than Helicobacter pylori in the Gastric Carcinogenesis[J].Helicobacter,2016,21(5):364-374.
[13]刘伟,张学智,李宁,等.荆花胃康胶丸及其主要成分土荆芥对于不同耐药Hp菌株杀菌作用的实验研究[C].中华中医药学会脾胃病分会第二十三次全国脾胃病学术交流会,2011.
[14]Qin J,Li Y,Cai Z,et al.A metagenome-wide association study of gut microbiota in type 2 diabetes[J].Nature,2012,490(7418):55-60.
[15]Le Chatelier E,Nielsen T,Qin J,et al.Richness of human gut microbiome correlates with metabolic markers[J].Nature,2013,500(7464):541-546.
[16]Feng Q,Liang S,Jia H,et al.Gut microbiome development along the colorectal adenoma-carcinoma sequence[J].Nat Commun,2015,6:6528.
[17]Jakobsson HE,Jernberg C,Andersson AF,et al.ShortTerm Antibiotic Treatment Has Differing Long-Term Impacts on the Human Throat and Gut Microbiome[J].PLo S One,2010,5(3):e9836.
[18]Ge XL,Ding C,Zhao W,et al.Antibiotics-induced depletion of mice microbiota induces changes in host serotonin biosynthesis and intestinal motility[J].Journal Of Translational Medicine,2017,15(1):13.
[19]Yang I,Woltemate S,Piazuelo MB,et al.Different gastric microbiota compositions in two human populations with high and low gastric cancer risk in Colombia[J].Sci Rep,2016,6:18594.
[20]Wroblewski LE,Peek RM,Jr.Helicobacter pylori,Cancer,and the Gastric Microbiota[J].Adv Exp Med Biol,2016,908:393-408.
[21]Brawner KM,Kumar R,Serrano CA,et al.Helicobacter pylori infection is associated with an altered gastric microbiota in children[J].Mucosal Immunol,2017,10(5):1169-1177.
[22]Neut C,Mahieux S,Dubreuil LJ.Antibiotic susceptibility of probiotic strains:Is it reasonable to combine probiotics with antibiotics[J].Med Mal Infect,2017,47(7):477.
[23]Bhatt AP,Redinbo MR,Bultman SJ.The Role of the Microbiome in Cancer Development and Therapy[J].Caa Cancer Journal For Clinicians,2017,67(4):327-344.
[2]Alarcon T,Llorca L,Perez-Perez G.Impact of the Microbiota and Gastric Disease Development by Helicobacter pylori[J].Curr Top Microbiol Immunol,2017,400:253-275.
[3]Yang YJ,Sheu BS.Metabolic Interaction of Helicobacter pylori Infection and Gut Microbiota[J].Microorganisms,2016,4(1):247-254.
[4]Liu G,Xie J,Lu ZR,et al.Fifth Chinese national consensus report on the management of Helicobacter pylori infection[J].Zhonghua Nei Ke Za Zhi,2017,56(7):532-545.
[5]Espinoza JL,Matsumoto A,Tanaka H,et al.Gastric microbiota:An emerging player in Helicobacter pyloriinduced gastric malignancies[J].Cancer Lett,2018,414:147-152.
[6]Petra CV,Rus A,Dumitrascu DL.Gastric microbiota:tracing the culprit[J].Clujul Med,2017,90(4):369-376.
[7]叶晖,于靖,张学智.土荆芥挥发油对小鼠体内幽门螺杆菌清除作用及对NF-κB表达的影响[J].中华中医药杂志,2017,32(12):5346-5349.
[8]张月苗,叶晖,王婷婷,等.荆花胃康胶丸联合三联疗法治疗幽门螺杆菌感染的1年随访研究[J].现代中医临床,2015,22(2):8-11.
[9]叶晖,李宁,于靖,等.荆花胃康胶丸对幽门螺杆菌感染小鼠胃黏膜核因子κB p65表达的影响[J].中国中医药信息杂志,2015,22(2):60-63.
[10]史宗明,叶晖,张学智.中成药研究现状的文献可视化分析---以荆花胃康胶丸为例[J].中华中医药学刊,2018,36(3):586-590.
[11]叶晖,李宁,于靖,等.昆明小鼠感染幽门螺杆菌动物模型的建立[J].胃肠病学和肝病学杂志,2015,24(3):284-286.
[12]Jo HJ,Kim J,Kim N,et al.Analysis of Gastric Microbiota by Pyrosequencing:Minor Role of Bacteria Other Than Helicobacter pylori in the Gastric Carcinogenesis[J].Helicobacter,2016,21(5):364-374.
[13]刘伟,张学智,李宁,等.荆花胃康胶丸及其主要成分土荆芥对于不同耐药Hp菌株杀菌作用的实验研究[C].中华中医药学会脾胃病分会第二十三次全国脾胃病学术交流会,2011.
[14]Qin J,Li Y,Cai Z,et al.A metagenome-wide association study of gut microbiota in type 2 diabetes[J].Nature,2012,490(7418):55-60.
[15]Le Chatelier E,Nielsen T,Qin J,et al.Richness of human gut microbiome correlates with metabolic markers[J].Nature,2013,500(7464):541-546.
[16]Feng Q,Liang S,Jia H,et al.Gut microbiome development along the colorectal adenoma-carcinoma sequence[J].Nat Commun,2015,6:6528.
[17]Jakobsson HE,Jernberg C,Andersson AF,et al.ShortTerm Antibiotic Treatment Has Differing Long-Term Impacts on the Human Throat and Gut Microbiome[J].PLo S One,2010,5(3):e9836.
[18]Ge XL,Ding C,Zhao W,et al.Antibiotics-induced depletion of mice microbiota induces changes in host serotonin biosynthesis and intestinal motility[J].Journal Of Translational Medicine,2017,15(1):13.
[19]Yang I,Woltemate S,Piazuelo MB,et al.Different gastric microbiota compositions in two human populations with high and low gastric cancer risk in Colombia[J].Sci Rep,2016,6:18594.
[20]Wroblewski LE,Peek RM,Jr.Helicobacter pylori,Cancer,and the Gastric Microbiota[J].Adv Exp Med Biol,2016,908:393-408.
[21]Brawner KM,Kumar R,Serrano CA,et al.Helicobacter pylori infection is associated with an altered gastric microbiota in children[J].Mucosal Immunol,2017,10(5):1169-1177.
[22]Neut C,Mahieux S,Dubreuil LJ.Antibiotic susceptibility of probiotic strains:Is it reasonable to combine probiotics with antibiotics[J].Med Mal Infect,2017,47(7):477.
[23]Bhatt AP,Redinbo MR,Bultman SJ.The Role of the Microbiome in Cancer Development and Therapy[J].Caa Cancer Journal For Clinicians,2017,67(4):327-344.