茉莉酸甲酯对鹅掌草中三萜皂苷anhuienoside E含量积累的影响
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摘要
目的观察茉莉酸甲酯对鹅掌草根部三萜皂苷含量积累影响。方法分别以5mmol/L,0.5mmol/L,0.05mmol/L 3种浓度茉莉酸甲酯喷施于鹅掌草植株根部,连续4周后,利用液相质谱联用技术,研究茉莉酸甲酯对鹅掌草三蔽皂苷anhuienosideE含量积累影响。结果不同水平茉莉酸甲酯对鹅掌草三蔽皂苷anhuienosideE含量积累均表现为促进作用,其中,以0.5mmol/L为最佳浓度水平。结论外施茉莉酸甲酯能在一定程度上促进鹅掌草三萜皂苷anhuienosideE含量的积累。
Objective To observe the effects of methy jasmonate on the content accumulation of triterpenoid saponin anhuienoside E in the roots of Anemone flaccida. Methods The roots of Anemone flaccida were sprayed with methy jasmonate(MeJA) at concentrations of 5 mmol/L, 0.5 mmol/L, 0.05 mmol/L for 4 weeks. The effect of methyl jasmonate on the accumulation of triterpenoid saponins anhuienoside E was studied by liquid phase mass spectrometry(LC-MS). Results The content of triterpenoid saponin anhuienoside E in Anemone flaccida sprayed with different levels of methyl jasmonate were promoted,among which 0.5 mmol/L was the best concentration.
Objective To observe the effects of methy jasmonate on the content accumulation of triterpenoid saponin anhuienoside E in the roots of Anemone flaccida. Methods The roots of Anemone flaccida were sprayed with methy jasmonate(MeJA) at concentrations of 5 mmol/L, 0.5 mmol/L, 0.05 mmol/L for 4 weeks. The effect of methyl jasmonate on the accumulation of triterpenoid saponins anhuienoside E was studied by liquid phase mass spectrometry(LC-MS). Results The content of triterpenoid saponin anhuienoside E in Anemone flaccida sprayed with different levels of methyl jasmonate were promoted,among which 0.5 mmol/L was the best concentration.
引文
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[16]Wang W,Zhong JJ.Manipulation of Ginsenoside Heterogeneity in Cell Cultures of Panax notoginseng by Addition ofJasmonates[J].Jurnal of Bioscience and Bioengineering,2002,93(1):48-53.
[17]Hu FX,Zhong JJ.Jasmonic acid mediates gene tran-scription of ginsenoside biosynthesis in cell cultures of Panax notoginseng treated with chemically synthesized 2-hydroxyethyl jas-monate[J].Process Biochemistry,2008(43):113-118.
[18]Claudio DO,Agnieszka C,Christopher D.Induction of secondary metabolism in grape cell cultures by jasmonates[J].Functional Plant Biology,2009(36):323-338.
[2]Han L T,Fang Y,Cao Y,et al.Triterpenoid Saponin Flaccidoside II from the Anemone flaccida triggers apoptosis ofNF1-associated malignant peripheral nerve sheath tumors via the MAPK-HO-1 Pathway[J].OncoTargets and Therapy,2016,32(9):1969-1979.
[3]Huang XJ,Tang JQ,Li MM,et al.Triterpenoid saponins from the rhizomes of Anemoneflaccida and their inhibitory activit ies on LPS-induced NO production in macrophageRAW264.7cells[J].J Asian Nat Prod Res,2014,16(9):910-921.
[4]马小月,韩璐,吴佳地,等.银莲花属植物资源概况及其综合利用[J].亚太传统医药,2014,10(19):39-41.
[5]高颖,郭鹏,白淑芳,等.多被银莲花的化学成分与药理作用[J].现代药物与临床,2009,24(2):91-95.
[6]邹忠杰,刘红霞,杨峻山,等.银莲花属植物化学成分及其药理活性[J].中国药学杂志,2004,39(7):493-495.
[7]程晓华,熊玉卿.五环三萜皂苷的药理作用研究进展[J].中草药,2007(5):792-795.
[8]Bonfill M,Mangas S,Moyano E,et al.Production of centellosides andphytosterols in cell suspension cultures of Centella asiatica[J].Plant CellTiss Organ Cult,2011(104):61-67.
[9]Kim OT,Kim MY,Huh SM,et al.Cloning of a c DNA probably en-coding oxidosqualene cyclase associated with asiaticoside biosynthesisfrom Centella asiatica[J].Plant Cell Rep,2005(24):304-311.
[10]朱宏涛,李江,李元,等.茉莉酸甲酯对三七组培苗中总皂苷含量的影响[J].西部林业科学,2014,43(2):72-78.
[11]谢阳姣,何志鹏,林伟.茉莉酸甲酯对苦玄参生长及苦玄参苷积累的影响研究[J].作物杂志,2013(2):80-84.
[12]唐京群.林荫银莲花的三萜皂苷类成分研究[D].广州:暨南大学,2013.
[13]邴飞虹,韩林涛,张国斌,等.蜈蚣三七的化学成分及免疫抑制活性[J].中国药科大学学报学,2008,39(6):496-499.
[14]Han LT,Li J,Huang F,et al.Triterpenoid saponins from Anemone flaccida induce apoptosis activity in He La cells[J].Asian Nat Prod Res,2009,11(2):122-127.
[15]韩林涛,黄芳,周云云,等.鹅掌草根茎主要皂苷成分的含量测定[J].时珍国医国药,2009,20(12):2930-2932.
[16]Wang W,Zhong JJ.Manipulation of Ginsenoside Heterogeneity in Cell Cultures of Panax notoginseng by Addition ofJasmonates[J].Jurnal of Bioscience and Bioengineering,2002,93(1):48-53.
[17]Hu FX,Zhong JJ.Jasmonic acid mediates gene tran-scription of ginsenoside biosynthesis in cell cultures of Panax notoginseng treated with chemically synthesized 2-hydroxyethyl jas-monate[J].Process Biochemistry,2008(43):113-118.
[18]Claudio DO,Agnieszka C,Christopher D.Induction of secondary metabolism in grape cell cultures by jasmonates[J].Functional Plant Biology,2009(36):323-338.