初治肺结核患者外周血Th22和Th17细胞及其细胞因子水平变化
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摘要
目的探讨Th22细胞和Th17细胞及其细胞因子水平在初治肺结核患者中的变化。方法选取80例初治肺结核患者,根据有无空洞分为有空洞组(n=33)和无空洞组(n=47),选取健康体检者76例为对照组,对所有入选者进行Th17细胞和Th22细胞数量及血浆IL-22和IL-17A浓度等测定。结果与对照组相比,初治肺结核患者外周血Th22细胞、Th17细胞数量、血浆IL-22和IL-17A水平均降低(P<0.05);有空洞组外周血Th22细胞、Th17细胞数量、血浆IL-22和IL-17A水平均低于无空洞组(P<0.05);Pearson相关提示在初治肺结核患者中,Th22细胞数量与Th17细胞数量呈正相关(r=0.286,P=0.006),血浆IL-22水平与Th22细胞数量、Th17细胞数量均呈正相关(r值分别为0.295、0.193,P<0.05)。结论外周血Th22和Th17细胞及其细胞因子水平与肺结核的发病和严重程度密切相关,对结核免疫反应起重要调节作用。
Objective To explore the changes of T helper(Th) 22 and Th17 cells and their cytokines in the pathogenesis of initial treatment pulmonary tuberculosis. Methods A total of 80 pulmonary tuberculosis patients with initial treatment were enrolled in the study. They were divided into cavitations group(n=33) and non cavitations group(n=47). 76 healthy people were selected as control group. Their frequencies of Th22 and Th17 cells in peripheral whole blood and interleukin(IL)-22 and IL-17 A in plasma were determined. Results The percentage of Th22 and Th17 cells, plasma IL-22 level and plasma IL-17 A level in pulmonary tuberculosis patients with initial treatment were all lower than the control group(P<0.05). The percentage of Th22 and Th17 cells, plasma IL-22 level and plasma IL-17 A level in cavitations group were all lower than that in non cavitations group(P<0.05). There was a positive correlation between circulating Th22 and Th17 cells in pulmonary tuberculosis patients with initial treatment(r=0.286, P=0.006). Plasma IL-22 level was positively correlated with Th22 cells and Th17 cells(r=0.295, r=0.193, P<0.05). Conclusion Th22 and Th17 cells and their cytokines are correlated with pathogenesis and progression of pulmonary tuberculosis. They can play important roles in immune function of patients with pulmonary tuberculosis.
Objective To explore the changes of T helper(Th) 22 and Th17 cells and their cytokines in the pathogenesis of initial treatment pulmonary tuberculosis. Methods A total of 80 pulmonary tuberculosis patients with initial treatment were enrolled in the study. They were divided into cavitations group(n=33) and non cavitations group(n=47). 76 healthy people were selected as control group. Their frequencies of Th22 and Th17 cells in peripheral whole blood and interleukin(IL)-22 and IL-17 A in plasma were determined. Results The percentage of Th22 and Th17 cells, plasma IL-22 level and plasma IL-17 A level in pulmonary tuberculosis patients with initial treatment were all lower than the control group(P<0.05). The percentage of Th22 and Th17 cells, plasma IL-22 level and plasma IL-17 A level in cavitations group were all lower than that in non cavitations group(P<0.05). There was a positive correlation between circulating Th22 and Th17 cells in pulmonary tuberculosis patients with initial treatment(r=0.286, P=0.006). Plasma IL-22 level was positively correlated with Th22 cells and Th17 cells(r=0.295, r=0.193, P<0.05). Conclusion Th22 and Th17 cells and their cytokines are correlated with pathogenesis and progression of pulmonary tuberculosis. They can play important roles in immune function of patients with pulmonary tuberculosis.
引文
[1] Amelio P, Portevin D, Reither K, et al. Mixed Th1 and Th2 Mycobacterium tuberculosis-specific CD4 T cell responses in patients with active pulmonary tuberculosis from Tanzania[J]. PLoS Negl Trop Dis, 2017, 11(7): e0005817.
[2] da Silva MV, Tiburcio MG, Machado JR, et al. Complexity and controversies over the cytokine profiles of T helper cell subpopulations in tuberculosis[J]. J Immunol Res, 2015, 2015: 639107.
[3] Arj A, Razavizadeh M, Mohammadi H, et al. The correlation between the numerical status of Th22 cells and serum level of IL-22 with severity of ulcerative colitis[J]. Iran J Allergy Asthma Immunol, 2018, 17(1): 78-84.
[4] 高孟秋.《WS 288—2017肺结核诊断》新标准中关于临床诊断患者判定的解析[J]. 中国防痨杂志, 2018, 40(3): 243-246.
[5] 张展, 刘慧, 石瑛, 等. 辅助性T22细胞和辅助性T17细胞在重度子痫前期发病中的作用[J]. 中华围产医学杂志, 2017, 20(1): 10-14.
[6] da Silva MV, Massaro Junior VJ, et al. Expression pattern of transcription factors and intracellular cytokines reveals that clinically cured tuberculosis is accompanied by an increase in Mycobacterium-specific Th1, Th2, and Th17 cells[J]. Biomed Res Int, 2015, 2015: 591237.
[7] 程涛, 伍伟玲, 黄河. 肺结核患者Thl/Th2/Treg/Th17免疫应答的临床研究[J].中国医药导报, 2017, 14(26): 109-112.
[8] Shen H, Chen ZW. The crucial roles of Th17-related cytokines/signal pathways in M. tuberculosis infection[J]. Cell Mol Immunol, 2018, 15(3): 216-225.
[9] Shu CC, Wu MF, Wang JY, et al. Decreased T helper 17 cells in tuberculosis is associated with increased percentages of programmed death ligand 1, T helper 2 and regulatory T cells[J]. Respir Res, 2017, 18(1): 128.
[10] 武忠长. 抗结核治疗对肺结核患者 Th17/Treg的影响[J]. 临床肺科杂志, 2016, 21(11): 2101-2104.
[11] Heidarnezhad F, Asnaashari A, Rezaee SA, et al. Evaluation of Interleukin17and Interleukin 23 expression in patients with active and latent tuberculosis infection[J]. Iran J Basic Med Sci, 2016, 19(8): 844-850.
[12] Shabgah AG, Navashenaq JG, Shabgah OG, et al. Interleukin-22 in human inflammatory diseases and viral infections[J]. Autoimmun Rev, 2017, 16(12): 1209-1218.
[13] 武忠长. 肺结核患者治疗前后外周血中IL-10、IL-22、IL-23水平变化及其临床意义[J]. 国际医药卫生导报, 2016, 22(10): 1457-1459.
[14] 梁华, 孙国宏, 余艺萍, 等. 肺结核病人血清中IL-22和IL-17的表达水平及临床意义分析[J]. 实验与检验医学, 2016, 34(5): 553-556.
[15] González-Amaro R, Marazuela M. Pathogenic Th17 and Th22 cells are increased in patients with autoimmune thyroid disorders[J]. Endocrine, 2017, 57(3): 409-417.
[2] da Silva MV, Tiburcio MG, Machado JR, et al. Complexity and controversies over the cytokine profiles of T helper cell subpopulations in tuberculosis[J]. J Immunol Res, 2015, 2015: 639107.
[3] Arj A, Razavizadeh M, Mohammadi H, et al. The correlation between the numerical status of Th22 cells and serum level of IL-22 with severity of ulcerative colitis[J]. Iran J Allergy Asthma Immunol, 2018, 17(1): 78-84.
[4] 高孟秋.《WS 288—2017肺结核诊断》新标准中关于临床诊断患者判定的解析[J]. 中国防痨杂志, 2018, 40(3): 243-246.
[5] 张展, 刘慧, 石瑛, 等. 辅助性T22细胞和辅助性T17细胞在重度子痫前期发病中的作用[J]. 中华围产医学杂志, 2017, 20(1): 10-14.
[6] da Silva MV, Massaro Junior VJ, et al. Expression pattern of transcription factors and intracellular cytokines reveals that clinically cured tuberculosis is accompanied by an increase in Mycobacterium-specific Th1, Th2, and Th17 cells[J]. Biomed Res Int, 2015, 2015: 591237.
[7] 程涛, 伍伟玲, 黄河. 肺结核患者Thl/Th2/Treg/Th17免疫应答的临床研究[J].中国医药导报, 2017, 14(26): 109-112.
[8] Shen H, Chen ZW. The crucial roles of Th17-related cytokines/signal pathways in M. tuberculosis infection[J]. Cell Mol Immunol, 2018, 15(3): 216-225.
[9] Shu CC, Wu MF, Wang JY, et al. Decreased T helper 17 cells in tuberculosis is associated with increased percentages of programmed death ligand 1, T helper 2 and regulatory T cells[J]. Respir Res, 2017, 18(1): 128.
[10] 武忠长. 抗结核治疗对肺结核患者 Th17/Treg的影响[J]. 临床肺科杂志, 2016, 21(11): 2101-2104.
[11] Heidarnezhad F, Asnaashari A, Rezaee SA, et al. Evaluation of Interleukin17and Interleukin 23 expression in patients with active and latent tuberculosis infection[J]. Iran J Basic Med Sci, 2016, 19(8): 844-850.
[12] Shabgah AG, Navashenaq JG, Shabgah OG, et al. Interleukin-22 in human inflammatory diseases and viral infections[J]. Autoimmun Rev, 2017, 16(12): 1209-1218.
[13] 武忠长. 肺结核患者治疗前后外周血中IL-10、IL-22、IL-23水平变化及其临床意义[J]. 国际医药卫生导报, 2016, 22(10): 1457-1459.
[14] 梁华, 孙国宏, 余艺萍, 等. 肺结核病人血清中IL-22和IL-17的表达水平及临床意义分析[J]. 实验与检验医学, 2016, 34(5): 553-556.
[15] González-Amaro R, Marazuela M. Pathogenic Th17 and Th22 cells are increased in patients with autoimmune thyroid disorders[J]. Endocrine, 2017, 57(3): 409-417.