10个高粱品系叶片不同部位SPAD值的比较分析
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摘要
为确定高粱叶片SPAD值的最佳测定位置,利用叶绿素计(SPAD-502Plus)对10个高粱恢复系同一叶片的叶尖部、叶中部、叶基部进行SPAD值测定,分析叶片不同部位SPAD值的分布及稳定性。结果表明,高粱品系HR的SPAD值最高,J9的SPAD值最低;10个高粱品系叶片不同测定位置上的SPAD值表现出一致的变化规律,即叶片中部>叶片尖部>叶片基部;叶片中部SPAD值的变异系数最小,数值相对稳定,可作为这10个高粱品系叶片SPAD值的适宜测定位置。表明叶片中部为较合适的测定部位,其SPAD值能较好地代表整个叶片的总叶绿素含量。
To determine the optimal location of SPAD value of sorghum leaves,SPAD values of leaf tip,leaf middle and leaf bottom on the same leaf were measured using the equipment SPAD-502 Plus for the sorghum cultivars. The results indicated that the SPAD value of HR was the highest,and J9 had the lowest SPAD value. The SPAD value of 10 sorghum lines at different positions showed a consistent change pattern,following the order:the middle leaf>the tip leaf>the base leaf. The coefficient of variation of SPAD value in the middle leaves was the least,and the values were relatively stable,which could be used as a suitable location for the SPAD value of the 10 sorghum lines. Therefore, the middle leaf is suitable measuring position, when using SPAD to evaluate the chlorophyll of sorghum leaf.
To determine the optimal location of SPAD value of sorghum leaves,SPAD values of leaf tip,leaf middle and leaf bottom on the same leaf were measured using the equipment SPAD-502 Plus for the sorghum cultivars. The results indicated that the SPAD value of HR was the highest,and J9 had the lowest SPAD value. The SPAD value of 10 sorghum lines at different positions showed a consistent change pattern,following the order:the middle leaf>the tip leaf>the base leaf. The coefficient of variation of SPAD value in the middle leaves was the least,and the values were relatively stable,which could be used as a suitable location for the SPAD value of the 10 sorghum lines. Therefore, the middle leaf is suitable measuring position, when using SPAD to evaluate the chlorophyll of sorghum leaf.
引文
[1]许大全.光合作用学[M].北京:科学出版社,2013:89-91.
[2]TRACY S,HAWKINS,EMILE S,et al.Modeling the relationship between extractable chlorophyll and SPAD-502 readings for endangered plant species research[J].Journal for Nature Conservation,2009,17:123-127.
[3]鱼欢,祖超,杨建峰,等.应用SPAD叶绿素仪测定不同位置胡椒叶片的SPAD值[J].热带作物学报,2012,33(10):1890-1895.
[4]赵犇,姚霞,田永超,等.基于上部叶片SPAD值估算小麦氮营养指数[J].生态学报,2013,33(3):916-924.
[5]蹇黎,朱利泉,张以忠,等.贵州兰花SPAD值和叶绿素含量测定与分析[J].安徽农业科学,2009,37(35):17462-17464.
[6]张丽丽,王璞,杨海龙,等.氮肥对夏玉米群体构建及光合特性的影响[J].华北农学报,2013,28(Z):332-336.
[7]刘莹,史树德.不同施氮水平下甜菜光合特性比较[J].北方农业学报,2016,44(2):7-12.
[8]陈晓群,张学军,白建忠,等.基于水稻不同生育期叶绿素值推荐追施氮量的研究初报[J].中国农学通报,2010,26(7):147-151.
[9]李旭华,扈强,潘义宏,等.不同成熟度烟叶叶绿素含量及其与SPAD值的相关分析[J].河南农业科学,2014,43(3):47-52,58.
[10]陈琴,陈莉敏,郑群英,等.5种牧草叶片上不同部位的SPAD值比较[J].草业科学,2014,31(7):1318-1322.
[11]柯娴氡,贺立静,苏志尧.南方4种木本植物相对叶绿素指标及其分布[J].中南林业科技大学学报,2010,30(8):82-86.
[12]李志宏,刘宏斌,张福锁.应用叶绿素仪诊断冬小麦氮营养状况的研究[J].植物营养与肥料学报,2003,9(4):401-405.
[13]冯国郡,章建新,李宏琪,等.甜高粱高光效种质的筛选和生理生化指标的比较[J].吉林农业大学学报,2013,25(3):260-268,277.
[14]张日清,何小燕,叶航,等.油茶叶片SPAD值差异的初步研究[J].中南林业科技大学学报,2011,31(3):30-33.
[15]赵士诚,何萍,仇少君,等.相对SPAD值用于不同品种夏玉米氮肥管理的研究[J].植物营养与肥料学报,2011,17(5):1091-1098.
[16]李志宏,张云贵,刘宏斌,等.叶绿素仪在夏玉米氮营养诊断中的应用[J].植物营养与肥料学报,2005,11(6):764-768.
[17]朱新开,盛海君,顾晶,等.应用SPAD值预测小麦叶片叶绿素和氮含量的初步研究[J].麦类作物学报,2005,25(2):46-50.
[18]屈卫群,王绍华,陈兵林,等.棉花主茎叶SPAD值与氮素营养诊断研究[J].作物学报,2007,33(6):1010-1017.
[19]李利,陈俊辉,樊明寿.使用叶绿素仪进行马铃薯氮素营养诊断的样本数确定[J].作物杂志,2015(4):135-137.
[20]PAGOLA M,ORTIZ R,IRIGOYEN I,et al.New method to assess barley nitrogen nutrition status based on image colour analysis comparison with SPAD-502[J].Computers and Electronics in Agriculture,2009,65:213-218.
[21]杨虎,戈长水,应武,等.遮荫对水稻冠层叶片SPAD值及光合形态特性参数的影响[J].植物营养与肥料学报,2014,20(3):580-587.
[22]郭建文.玉米叶片SPAD值在除草剂药害早期诊断中的应用[J].山西农业科学,2013,41(1):86-88,91.
[23]高飞,肖靖,谷运红,等.利用小麦叶片SPAD值预测成熟期籽粒蛋白质含量的研究[J].光谱学与光谱分析,2012,32(5):1350-1354.
[24]SONGSRI P,JOGLOY S,HOLBROOK C,et al.Association of root,specific leaf area and SPAD chlorophyll meter reading to water use efficiency of peanut under different available soil water[J].Agricultural Water Management,2009,96:790-798.
[25]ZHANG D Y,WANG X H,CHEN Y,et al.Determinant of photosynthesis capacity in rice leaves under ambient air conditions[J].Photosynthetica,2005,43:135-137.
[2]TRACY S,HAWKINS,EMILE S,et al.Modeling the relationship between extractable chlorophyll and SPAD-502 readings for endangered plant species research[J].Journal for Nature Conservation,2009,17:123-127.
[3]鱼欢,祖超,杨建峰,等.应用SPAD叶绿素仪测定不同位置胡椒叶片的SPAD值[J].热带作物学报,2012,33(10):1890-1895.
[4]赵犇,姚霞,田永超,等.基于上部叶片SPAD值估算小麦氮营养指数[J].生态学报,2013,33(3):916-924.
[5]蹇黎,朱利泉,张以忠,等.贵州兰花SPAD值和叶绿素含量测定与分析[J].安徽农业科学,2009,37(35):17462-17464.
[6]张丽丽,王璞,杨海龙,等.氮肥对夏玉米群体构建及光合特性的影响[J].华北农学报,2013,28(Z):332-336.
[7]刘莹,史树德.不同施氮水平下甜菜光合特性比较[J].北方农业学报,2016,44(2):7-12.
[8]陈晓群,张学军,白建忠,等.基于水稻不同生育期叶绿素值推荐追施氮量的研究初报[J].中国农学通报,2010,26(7):147-151.
[9]李旭华,扈强,潘义宏,等.不同成熟度烟叶叶绿素含量及其与SPAD值的相关分析[J].河南农业科学,2014,43(3):47-52,58.
[10]陈琴,陈莉敏,郑群英,等.5种牧草叶片上不同部位的SPAD值比较[J].草业科学,2014,31(7):1318-1322.
[11]柯娴氡,贺立静,苏志尧.南方4种木本植物相对叶绿素指标及其分布[J].中南林业科技大学学报,2010,30(8):82-86.
[12]李志宏,刘宏斌,张福锁.应用叶绿素仪诊断冬小麦氮营养状况的研究[J].植物营养与肥料学报,2003,9(4):401-405.
[13]冯国郡,章建新,李宏琪,等.甜高粱高光效种质的筛选和生理生化指标的比较[J].吉林农业大学学报,2013,25(3):260-268,277.
[14]张日清,何小燕,叶航,等.油茶叶片SPAD值差异的初步研究[J].中南林业科技大学学报,2011,31(3):30-33.
[15]赵士诚,何萍,仇少君,等.相对SPAD值用于不同品种夏玉米氮肥管理的研究[J].植物营养与肥料学报,2011,17(5):1091-1098.
[16]李志宏,张云贵,刘宏斌,等.叶绿素仪在夏玉米氮营养诊断中的应用[J].植物营养与肥料学报,2005,11(6):764-768.
[17]朱新开,盛海君,顾晶,等.应用SPAD值预测小麦叶片叶绿素和氮含量的初步研究[J].麦类作物学报,2005,25(2):46-50.
[18]屈卫群,王绍华,陈兵林,等.棉花主茎叶SPAD值与氮素营养诊断研究[J].作物学报,2007,33(6):1010-1017.
[19]李利,陈俊辉,樊明寿.使用叶绿素仪进行马铃薯氮素营养诊断的样本数确定[J].作物杂志,2015(4):135-137.
[20]PAGOLA M,ORTIZ R,IRIGOYEN I,et al.New method to assess barley nitrogen nutrition status based on image colour analysis comparison with SPAD-502[J].Computers and Electronics in Agriculture,2009,65:213-218.
[21]杨虎,戈长水,应武,等.遮荫对水稻冠层叶片SPAD值及光合形态特性参数的影响[J].植物营养与肥料学报,2014,20(3):580-587.
[22]郭建文.玉米叶片SPAD值在除草剂药害早期诊断中的应用[J].山西农业科学,2013,41(1):86-88,91.
[23]高飞,肖靖,谷运红,等.利用小麦叶片SPAD值预测成熟期籽粒蛋白质含量的研究[J].光谱学与光谱分析,2012,32(5):1350-1354.
[24]SONGSRI P,JOGLOY S,HOLBROOK C,et al.Association of root,specific leaf area and SPAD chlorophyll meter reading to water use efficiency of peanut under different available soil water[J].Agricultural Water Management,2009,96:790-798.
[25]ZHANG D Y,WANG X H,CHEN Y,et al.Determinant of photosynthesis capacity in rice leaves under ambient air conditions[J].Photosynthetica,2005,43:135-137.