优化生活饮用水中微生物检测的处理方法
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摘要
目的优化生活饮用水微生物检测中总大肠菌群、耐热大肠菌群、大肠埃希氏菌滤膜法的稀释及过滤方法,并验证方法可行性。方法待测水样稀释1倍,旋转加入稀释液过滤,再依据国家标准GB/T5750.12-2006《生活饮用水标准检验方法微生物指标》滤膜法的条件,培养、验证;同时按照该方法处理能力验证待测水样,检验并上报结果。结果总大肠菌、耐热大肠菌、大肠埃希氏菌菌落在滤膜上生长均匀,可计数,能力验证结果|Z|均小于2。结论通过能力验证证实本实验稀释方法合理,过滤手段有效,适用于复杂水样总大肠菌群、耐热大肠菌群、大肠埃希氏菌的检测。
Objective To optimize the dilution and filtration methods of total coliform, heat-resistant coliform and Escherichia coli in the detection of microorganisms in drinking water, and verify the feasibility of the method.Methods The water sample to be tested was diluted 1 time, added to the dilution solution by rotation, and then cultured and verified according to the conditions of the national standard GB/T 5750.12-2006 Standard test method for drinking water standards microbiological index. The processing capacity of this method was performed to verify the water sample, and the results were reported. Results Total coliform, heat-resistant coliform and Escherichia coli colonies grew uniformly on the filter membrane and could be counted. The results of capacity verification showed that |Z| were all less than 2. Conclusion Through the verification of capability, it is proved that the dilution method of this experiment is reasonable and the filtration method is effective. It is suitable for the detection of total coliform bacteria, heat-resistant coliforms and Escherichia coli in complex water samples.
Objective To optimize the dilution and filtration methods of total coliform, heat-resistant coliform and Escherichia coli in the detection of microorganisms in drinking water, and verify the feasibility of the method.Methods The water sample to be tested was diluted 1 time, added to the dilution solution by rotation, and then cultured and verified according to the conditions of the national standard GB/T 5750.12-2006 Standard test method for drinking water standards microbiological index. The processing capacity of this method was performed to verify the water sample, and the results were reported. Results Total coliform, heat-resistant coliform and Escherichia coli colonies grew uniformly on the filter membrane and could be counted. The results of capacity verification showed that |Z| were all less than 2. Conclusion Through the verification of capability, it is proved that the dilution method of this experiment is reasonable and the filtration method is effective. It is suitable for the detection of total coliform bacteria, heat-resistant coliforms and Escherichia coli in complex water samples.
引文
[1]彭佳艳,赵永,郑维斌,等.2014~2016年保山市农村饮用水卫生状况监测分析[J].食品安全质量检测学报,2018,9(15):4175-4179.Peng JY,Zhao Y,Zhen WB,et al.Monitoring and analysis of drinking water hygiene status in rural areas of Baoshan city from 2014 to 2016[J].J Food Saf Qual,2018,9(15):4175-4179..
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[10]汪桃花,代真真,王新文,等.能力验证对提升实验室技术水平的影响[J].现代食品,2018,(16):19-22.Wang TH,Dai ZZ,Wang XW,et al.Impact of proficiency testing in technical level of the laboratory[J].Mod Food,2018,(16):19-22.
[11]劳嘉倩.浅谈食品检测实验室质量控制技术[J].广东化工,2018,21(45):56-57.Lao JQ.Technology of quality control in food testing laboratory[J].Guangdong Chem Ind,2018,21(45):56-57.
[12]袁磊,乔荣霞,杨洪,等.2017年食品检验实验室能力验证结果分析[J].食品安全质量检测学报,2018,20(9):5504-5510.Yuan L,Qiao RX,Yang H,et al.Proficiency testing results and analysis of food laboratories in 2017[J].J Food Saf Qual,2018,20(9):5504-5510.
[13]GB 4789.1-2016食品安全国家标准食品微生物学检验总则[S].GB 4789.1-2016 National food safety standard-General rules for food microbiological-Inspection[S].
[2]王舒,李继芳,崔仲明.2017年辽宁省农村饮用水中微生物污染状况调查[J].中国卫生工程学,2018,(6):255-256.Wang S,Li JF,Cui ZM.Investigation on microbial pollution in rural drinking water in Liaoning province in 2017[J].Chin J Public Health Eng,2018,(6):255-256.
[3]覃昆飞,曾肖寒,郑巨瑞.2015年-2017年贵港市覃塘区农村饮用水水质监测结果分析[J].中国卫生检验杂志,2018,28(19):2417-2419.Qin KF,Zeng XH,Zhen JR.Analysis of the monitoring results of rural drinking water quality in Qintang district,Guigang during 2015-2017[J].Chin J Health Leb Technol,2018,28(19):2417-2419.
[4]扎丽帕·热合曼,董宪春.生活饮用水水质微生物检验分析的重要性[J].世界最新医学信息文摘,2017,17(77):175.Zharipa RHM,Dong XC.Importance of microbial testing and analysis of drinking water quality[J].World's Latest Med Inf Abstract,2017,17(77):175.
[5]朱静鸿,龚云伟,赵红.399份生活饮用水微生物指标检测与分析[J].中国卫生产业,2015,23(37):37-39.Zhu JH,Gong YW,Zhao H.Detection and analysis of microbial indicators in 399 samples of drinking water[J].China Health Ind,2015,23(37):37-39.
[6]何国庆,张伟.食品微生物检验技术[M].北京:中国质检出版社,中国标准出版社,2013.He GQ,Zhang W.Food microbiological testing technology[M].Beijing:China Quality Inspection Press,China Standards Publishing House,2013.
[7]GB/T 5750.12-2006生活饮用水标准检验方法微生物指标[S].GB/T 5750.12-2006 Standard examination methods for dringking water--Microbiological parameters[S].
[8]史梦雨.生活饮用水中总大肠菌群的两种方法比较[J].中国城乡企业卫生,2017,10(10):139-140.Shi MY.Comparison of two methods for total coliforms in drinking water[J].Chin J Urban Rural Enter Hyg,2017,10(10):139-140.
[9]冯雪娇.关于水中总大肠菌群检测方法的探讨[J].黑龙江科技信息,2013,(14):32.Feng XJ.Discussion on detection method of total coliforms in water[J].Heilongjiang Sci Technol Inf,2013,(14):32.
[10]汪桃花,代真真,王新文,等.能力验证对提升实验室技术水平的影响[J].现代食品,2018,(16):19-22.Wang TH,Dai ZZ,Wang XW,et al.Impact of proficiency testing in technical level of the laboratory[J].Mod Food,2018,(16):19-22.
[11]劳嘉倩.浅谈食品检测实验室质量控制技术[J].广东化工,2018,21(45):56-57.Lao JQ.Technology of quality control in food testing laboratory[J].Guangdong Chem Ind,2018,21(45):56-57.
[12]袁磊,乔荣霞,杨洪,等.2017年食品检验实验室能力验证结果分析[J].食品安全质量检测学报,2018,20(9):5504-5510.Yuan L,Qiao RX,Yang H,et al.Proficiency testing results and analysis of food laboratories in 2017[J].J Food Saf Qual,2018,20(9):5504-5510.
[13]GB 4789.1-2016食品安全国家标准食品微生物学检验总则[S].GB 4789.1-2016 National food safety standard-General rules for food microbiological-Inspection[S].