多指标评价有机相中纳滤分离丹酚酸B的适用性
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摘要
目的探索纳滤技术分离有机相中丹酚酸B的适用性。方法以截留率、传质系数、膜通量、膜耐用性和完整性为指标,综合评价大孔树脂洗脱制备的有机相中纳滤分离丹酚酸B的适用性。结果丹酚酸B截留率随着膜截留相对分子质量增加而下降;随着溶液中乙醇比例增加,膜通量衰减明显且丹酚酸B传质系数下降。通过膜耐用性和完整性评价发现,随着分离时间的增加,NFG纳滤膜的截留性能稳定;在膜污染、添加剂溶解脱落等多种效应下,膜通量出现先下降进而升高并维持稳定的现象,同时膜分离层荷电特征稳定,但有效分离孔径增加。结论 NFG纳滤膜可以满足低浓度乙醇溶液中丹酚酸B的精制分离,具有较好的适用性。以丹酚酸B原料精制为例综合分析有机相中纳滤分离的适用性,为有机相中酚酸类成分的精制富集提供理论和技术支撑。
Objective To explore the applicability of nanofiltration separation of salvianolic acid B in organic phase. Methods The rejection rate, mass transfer coefficient, membrane flux, membrane durability and membrane integrity were selected as indexes to evaluate the applicability of nanofiltration for the separation of salvianolic acid B raw material in organic phase from the eluate of macroporous resin. Results The experiment showed that there was a negative relationship between rejection rate and membrane molecular weight cut-off. Meanwhile, the mass transfer coefficient of salvianolic acid B was decreased and the membrane flux was obviously decayed with the increase of ethanol concentration. Through the membrane durability and integrity evaluation, the rejection performance of NFG nanofiltration membrane was stable with the increasing of separation time. Also, the membrane flux decreased first and then increased and maintained stable. At the same time, the charge characteristic of the membrane separation layer was stable,but the effective separation aperture was increased by the combined effects of membrane fouling, additives shedding and so on.Conclusion Based on the comprehensive analysis, NFG nanofiltration membrane could meet the requirements of the separation of low concentration ethanol solution, and had good applicability. The applicability of nanofiltration separation in organic phase was analyzed comprehensively with salvianolic acid B as example, providing theoretical and technical support for nanofiltration separation of phenolic acids in organic phase.
Objective To explore the applicability of nanofiltration separation of salvianolic acid B in organic phase. Methods The rejection rate, mass transfer coefficient, membrane flux, membrane durability and membrane integrity were selected as indexes to evaluate the applicability of nanofiltration for the separation of salvianolic acid B raw material in organic phase from the eluate of macroporous resin. Results The experiment showed that there was a negative relationship between rejection rate and membrane molecular weight cut-off. Meanwhile, the mass transfer coefficient of salvianolic acid B was decreased and the membrane flux was obviously decayed with the increase of ethanol concentration. Through the membrane durability and integrity evaluation, the rejection performance of NFG nanofiltration membrane was stable with the increasing of separation time. Also, the membrane flux decreased first and then increased and maintained stable. At the same time, the charge characteristic of the membrane separation layer was stable,but the effective separation aperture was increased by the combined effects of membrane fouling, additives shedding and so on.Conclusion Based on the comprehensive analysis, NFG nanofiltration membrane could meet the requirements of the separation of low concentration ethanol solution, and had good applicability. The applicability of nanofiltration separation in organic phase was analyzed comprehensively with salvianolic acid B as example, providing theoretical and technical support for nanofiltration separation of phenolic acids in organic phase.
引文
[1]伍利华,黄英,刘婷,等.陶瓷膜分离技术应用于中药口服液的研究进展[J].药物评价研究,2014,37(2):184-187.
[2]Lively R P,Sholl D S.From water to organics in membrane separations[J].Nat Mater,2017,16(3):276-279.
[3]鞠爱春,耿诗涵,杨欣鹏,等.丹酚酸B鼻腔给药对脑缺血损伤大鼠学习记忆能力及神经再生的影响[J].中草药,2017,48(12):2481-2485.
[4]夏杨,张惠军,聂亚莉.丹酚酸B预处理对心肌缺血/再灌注损伤能量代谢的影响[J].药物评价研究,2018,41(12):2210-2213.
[5]陶善珺,任尤楠,赵梦秋,等.丹酚酸B对糖尿病血糖波动模型大鼠胰岛细胞的保护作用[J].中草药,2016,47(17):3058-3063.
[6]周丹,田天,舒庆,等.丹酚酸B对大鼠缺血心肌血管再生的促进作用[J].中草药,2018,49(21):5166-5169.
[7]Bódalo A,Gómez E,Hidalgo A M,et al.Nanofiltration membranes to reduce phenol concentration in waste water[J].Desalination,2009,245(1):680-686.
[8]Alexandru M A,Pauline M,Cindi B,et al.Concentrations of polyphenols from blueberry pomace extract using nanofiltration[J].Food Bioprod Process,2017,106:91-101.
[9]李存玉,马赟,刘莉成,等.紫苏叶中咖啡酸存在状态与其纳滤传质过程的相关性[J].中草药,2017,48(19):3986-3991.
[10]张建成,袁其朋,赵彦彦.有机溶剂透过纳滤膜的通量模型研究[J].北京化工大学学报:自然科学版,2006,33(6):93-95.
[11]李存玉,马赟,刘莉成,等.中药枳实中辛弗林存在状态与其纳滤传质过程的相关性[J].中国中药杂志,2017,42(23):4598-4603.
[12]张启伟,张颖,李计萍,等.高效液相色谱法测定丹参中丹酚酸B[J].中国中药杂志,2001,26(12):197-199.
[13]中国药典[S].一部.2015.
[14]李存玉,陈琪,刘乃榕,等.有机溶液环境中阿魏酸的纳滤“强化”分离行为研究[J].中草药,2018,49(21):5070-5075.
[15]Murthy Z V P,Gupta S K.Estimation of mass transfer coefficient using a combined nonlinear membrane transport and film theory model[J].Desalination,1997,109(1):39-49.
[16]赵彦彦,袁其朋.有机相纳滤分离过程中浓度、电荷、溶剂对溶质截留行为的影响[J].膜科学与技术,2006,26(5):31-36.
[17]李存玉,刘莉成,金立阳,等.基于道南效应和溶解-扩散效应分析低浓度乙醇中绿原酸的纳滤分离规律[J].中国中药杂志,2017,42(14):2670-2675.
[18]张泉,郭曦,董文艺,等.预处理方式对纳滤工艺性能及膜污染影响研究[J].膜科学与技术,2014,34(1):82-86.
[19]Zhou M Y,Fang L F,Sun C C,et al.Pore size tailoring from ultrafiltration to nanofiltration with PVC-g-PDMAvia rapid immersion thermal annealing[J].J Membr Sci,2018,572:401-409.
[20]蔡铭,吕雨晴,侯文忠,等.苹果汁纳滤过程的膜污染机制及多酚截留特性[J].中国食品学报,2018,18(1):104-112.
[21]Foureaux A F S,Reis E O,Lebron Y,et al.Rejection of pharmaceutical compounds from surface water by nanofiltration and reverse osmosis[J].Sep Purif Technol,2019,212:171-179.
[22]李存玉,马赟,龚柔佳,等.响应面分析法耦合调节Donnan效应优化苦参提取液的纳滤浓缩工艺[J].中草药,2016,47(19):3395-3400.
[23]金万勤,徐南平.限域传质分离膜[J].化工学报,2018,69(1):50-56.
[2]Lively R P,Sholl D S.From water to organics in membrane separations[J].Nat Mater,2017,16(3):276-279.
[3]鞠爱春,耿诗涵,杨欣鹏,等.丹酚酸B鼻腔给药对脑缺血损伤大鼠学习记忆能力及神经再生的影响[J].中草药,2017,48(12):2481-2485.
[4]夏杨,张惠军,聂亚莉.丹酚酸B预处理对心肌缺血/再灌注损伤能量代谢的影响[J].药物评价研究,2018,41(12):2210-2213.
[5]陶善珺,任尤楠,赵梦秋,等.丹酚酸B对糖尿病血糖波动模型大鼠胰岛细胞的保护作用[J].中草药,2016,47(17):3058-3063.
[6]周丹,田天,舒庆,等.丹酚酸B对大鼠缺血心肌血管再生的促进作用[J].中草药,2018,49(21):5166-5169.
[7]Bódalo A,Gómez E,Hidalgo A M,et al.Nanofiltration membranes to reduce phenol concentration in waste water[J].Desalination,2009,245(1):680-686.
[8]Alexandru M A,Pauline M,Cindi B,et al.Concentrations of polyphenols from blueberry pomace extract using nanofiltration[J].Food Bioprod Process,2017,106:91-101.
[9]李存玉,马赟,刘莉成,等.紫苏叶中咖啡酸存在状态与其纳滤传质过程的相关性[J].中草药,2017,48(19):3986-3991.
[10]张建成,袁其朋,赵彦彦.有机溶剂透过纳滤膜的通量模型研究[J].北京化工大学学报:自然科学版,2006,33(6):93-95.
[11]李存玉,马赟,刘莉成,等.中药枳实中辛弗林存在状态与其纳滤传质过程的相关性[J].中国中药杂志,2017,42(23):4598-4603.
[12]张启伟,张颖,李计萍,等.高效液相色谱法测定丹参中丹酚酸B[J].中国中药杂志,2001,26(12):197-199.
[13]中国药典[S].一部.2015.
[14]李存玉,陈琪,刘乃榕,等.有机溶液环境中阿魏酸的纳滤“强化”分离行为研究[J].中草药,2018,49(21):5070-5075.
[15]Murthy Z V P,Gupta S K.Estimation of mass transfer coefficient using a combined nonlinear membrane transport and film theory model[J].Desalination,1997,109(1):39-49.
[16]赵彦彦,袁其朋.有机相纳滤分离过程中浓度、电荷、溶剂对溶质截留行为的影响[J].膜科学与技术,2006,26(5):31-36.
[17]李存玉,刘莉成,金立阳,等.基于道南效应和溶解-扩散效应分析低浓度乙醇中绿原酸的纳滤分离规律[J].中国中药杂志,2017,42(14):2670-2675.
[18]张泉,郭曦,董文艺,等.预处理方式对纳滤工艺性能及膜污染影响研究[J].膜科学与技术,2014,34(1):82-86.
[19]Zhou M Y,Fang L F,Sun C C,et al.Pore size tailoring from ultrafiltration to nanofiltration with PVC-g-PDMAvia rapid immersion thermal annealing[J].J Membr Sci,2018,572:401-409.
[20]蔡铭,吕雨晴,侯文忠,等.苹果汁纳滤过程的膜污染机制及多酚截留特性[J].中国食品学报,2018,18(1):104-112.
[21]Foureaux A F S,Reis E O,Lebron Y,et al.Rejection of pharmaceutical compounds from surface water by nanofiltration and reverse osmosis[J].Sep Purif Technol,2019,212:171-179.
[22]李存玉,马赟,龚柔佳,等.响应面分析法耦合调节Donnan效应优化苦参提取液的纳滤浓缩工艺[J].中草药,2016,47(19):3395-3400.
[23]金万勤,徐南平.限域传质分离膜[J].化工学报,2018,69(1):50-56.
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