脑缺血预处理对大鼠蛛网膜下腔出血后细胞凋亡的影响
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摘要
目的观察脑缺血预处理对蛛网膜出血大鼠神经细胞凋亡的影响,探讨脑缺血预处理对蛛网膜下腔出血后脑损害的保护作用及机制。方法 78只雄性SD大鼠,随机分为5组:A组6只(正常对照组);B组18只(假手术组);C组18只(缺血预处理组);D组18只(SAH组);E组18只(脑缺血预处理3 d后SAH组)。采用改良四血管阻断法制备全脑缺血预处理模型,视交叉池注血法制备SAH模型。各实验组动物分别在l、3、7天进行神经行为学评分,并处死后取脑,行TUNEL染色,显微镜高倍视野下观察各组海马CA1区神经元凋亡状况。结果与A、B、C组相比较,D组神经行为学评分最高,海马CA1区神经元凋亡现象最明显,差异具有统计学意义(P<0.05),造模成功。E组与D组各时间点比较,神经行为学改变评分有所降低,海马CA1区神经元细胞凋亡现象较少,差异有统计学意义(P<0.05)。结论脑缺血预处理可以有效减轻大鼠SAH后的神经功能损害,其机制与避免神经细胞凋亡有关。
Objective To observe the effect of cerebral ischemic preconditioning(CIP)on neuronal apoptosis in rats with subarachnoid hemorrhage,and to explore the protective effect and mechanism of CIP on brain injury after subarachnoid hemorrhage(SAH). Methods Seventy-eight Sprague-Dawley male rats were randomly divided into 5 groups:group A(normal group,n=6),group B(sham operation,n=18),group C(CIP group,n=18),group D(SAH model group,n=18),group E(reperfusion for 3 days and SAH followed). Modified four-vessel occlusion method was used to replicate animal model of global cerebral ischemia and "auto blood injection into prechiasmatic cistern" method was used to replicate animal model of SAH. Neuroethology scores were detected in the 1 st,3 rd and 7 th day. Then the rats were decapitated in time and the brains were taken out. Results Neuron in hippocampal CA1 region of rat were observed by HE and TUNEL staining under the microscope. Neuroeology scores in group E was obviously lower than group D(P<0.05). Neuron apoptosis in hippocampal CA1 region of group E was obviously less than group D with significant difference(P<0.05). Conclusion Cerebral ischemic preconditioning can effectively alleviate the neurological impairment after SAH in rats,and its mechanism is related to the avoidance of neuronal apoptosis.
Objective To observe the effect of cerebral ischemic preconditioning(CIP)on neuronal apoptosis in rats with subarachnoid hemorrhage,and to explore the protective effect and mechanism of CIP on brain injury after subarachnoid hemorrhage(SAH). Methods Seventy-eight Sprague-Dawley male rats were randomly divided into 5 groups:group A(normal group,n=6),group B(sham operation,n=18),group C(CIP group,n=18),group D(SAH model group,n=18),group E(reperfusion for 3 days and SAH followed). Modified four-vessel occlusion method was used to replicate animal model of global cerebral ischemia and "auto blood injection into prechiasmatic cistern" method was used to replicate animal model of SAH. Neuroethology scores were detected in the 1 st,3 rd and 7 th day. Then the rats were decapitated in time and the brains were taken out. Results Neuron in hippocampal CA1 region of rat were observed by HE and TUNEL staining under the microscope. Neuroeology scores in group E was obviously lower than group D(P<0.05). Neuron apoptosis in hippocampal CA1 region of group E was obviously less than group D with significant difference(P<0.05). Conclusion Cerebral ischemic preconditioning can effectively alleviate the neurological impairment after SAH in rats,and its mechanism is related to the avoidance of neuronal apoptosis.
引文
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[2]Longa EZ,Weinstein PR,Carlson S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20:84-91.
[3]杨彬彬,唐晓平,赵龙,等.高压氧对大鼠蛛网膜下腔出血后迟发性脑血管痉挛的影响[J].中华临床医师杂志(电子版),2016,10(7):974-977.
[4]Murry CE,Jenings RS,et al.Preconditioning with ischemia dalay of lethal cell injury in myocardium[J].Circulation,1986,74(5):1124-1127.
[5]Kitagawa K,Matsumoto M,Masafumi Tagaya,et al."Ischemic tolerance" phenomenon found in the brain[J].Brain research,1990,528:21-24.
[6]Valentim LM,Geyer AB,Tavares A,et al.Effects of global cerebral ischemia and preconditioning on heat shock protein 27 immuno content and phosphorylation in rat hippocampus[J].Neuronscience,2001,107:43-44.
[7]李建民,赵雅宁,安超旺,等.脑缺血预处理对沙鼠前脑缺血再灌注后HSP70表达水平及学习记忆能力的影响[J]. 第二军医大学学报,2010,31(1):55-59.
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