基于双启动寡聚核苷酸引物的PCR技术及其研究应用
|
摘要
引物的设计是决定PCR反应能否特异性及高效地扩增的要素之一。双启动寡聚核苷酸(dual priming oligonucleotide,DPO)引物,是一种包含两个独立的特异性引物区域和寡聚次黄嘌呤(poly I)碱基桥连结构的设计方法。近年来,基于双启动寡聚核苷酸引物的PCR技术被广泛应用于临床医学检验、食源性病原微生物检验及动植物病毒检验检疫等领域,检验结果符合检验检疫行业标准。与常规PCR引物相比较,双启动寡核苷酸引物具有特异性更强,实用性良好等优点。本综述系统地综述了双启动寡聚核苷酸引物的设计原理及DPO-PCR技术在检验检疫方面的研究进展并探讨其应用价值。
Design of primers is one of the factors that determine whether PCR can be amplified efficiently and specifically. Dual priming oligonucleotide primer is a design method, which contains two separate specific primer domains and poly I bases joined by the bridge construction. The PCR technique with DPO primers is widely applied in the fields of clinical medical examination, food borne pathogenic microorganism inspection and inspection and quarantine of plant and animal viruses in recent years, and the test results meet the standards for inspection and quarantine. Compared with the conventional PCR primers, the specificity of the dual priming oligonucleotide primers are stronger and more practical. In this paper, the design principle of dual priming oligonucleotide primers and the research progress of DPO-PCR on inspection and quarantine were systematically reviewed, as well as its application value.
Design of primers is one of the factors that determine whether PCR can be amplified efficiently and specifically. Dual priming oligonucleotide primer is a design method, which contains two separate specific primer domains and poly I bases joined by the bridge construction. The PCR technique with DPO primers is widely applied in the fields of clinical medical examination, food borne pathogenic microorganism inspection and inspection and quarantine of plant and animal viruses in recent years, and the test results meet the standards for inspection and quarantine. Compared with the conventional PCR primers, the specificity of the dual priming oligonucleotide primers are stronger and more practical. In this paper, the design principle of dual priming oligonucleotide primers and the research progress of DPO-PCR on inspection and quarantine were systematically reviewed, as well as its application value.
引文
Bibby D.F.,Mcelarney I.,Breuer J.,and Clark D.A.,2011,Comparative evaluation of the seegene seeplex RV15 and real-time PCR for respiratory virus detection,Journal of Medical Virology,83(8):1469-1475
Carrara L.,Navarro F.,Turbau M.,Seres M.,Morán I.,Quintana I.,Martino R.,González Y.,Brell A.,Cordon O.,Diestra K.,Mata C.,Mirelis B.,and Coll P.,2013,Molecular diagnosis of bloodstream infections with a new dual-priming oligonucleotide-based multiplex PCR assay,Journal of Medical Microbiology,62:1673-1679
Chamberlain J.S.,Gibbs R.A.,Ranier J.E.,Nguyen P.N.,and Caskey C.T.,1988,Deletion screening of the duchenne muscular dystrophy locus via multiplex DNA amplification,Nucleic Acids Research,16(23):11141-11156
Chun J.Y.,Kim K.J.,Hwang I.T.,Kim Y.J.,Lee D.H.,Lee I.K.,an d Kim J.K.,2007,Dual priming oligonucleotide system for the multiplex detection of respiratory viruses and snp genotyping of CYP2C19 gene,Nucleic Acids Research,35(6):e40
Chung W.C.,Jung S.H.,Oh J.H.,Kim T.H.,Cheung D.Y.,Kim B.W.,Kim S.S.,Kim J.I.,and Sin E.Y.,2014,Dual-priming oligonucleotide-based multiplex PCR using tissue samples in rapid urease test in the detection of helicobacter pylori infection,World Journal of Gastroenterology,20(21):6547-6553
Chung W.C,Jeon E.J.,Oh J.H.,Park J.M.,Kim T.H.,Cheung D.Y.,Kim B.W.,Kim S.S.,and Kim J.I.,2016,Dual-priming oligonucleotide-based multiplex PCR using tissue samples from the rapid urease test kit for the detection of Helicobacter pylori in bleedingpeptic ulcers,Digestive and Liver Disease,48(8):899-903
Heid C.A.,Stevens J.,Livak K.J.,and Williams P.M.,1996,Real time quantitative PCR,Genome Research,6(10):986-994
Higuchi R.,Fockler C.,Dollinger G.,and Watson R.,1993,Kinetic pcr analysis:Real-time monitoring of DNA amplification reactions,Nature Biotechnolgy,11(9):1026-1030
Higuchi R.,Dollinger G.,Walsh P.S.,a nd Griffi th R.,1992,Simultaneous amplification and detection of specific DNA sequences,Bio/technolgy,10(4):413-417
Holland P.M.,Abramson R.D.,Watson R.,and Gelfand D.H.,1991,Detection ofspecific polymerase chain reaction product by utilizing the 5'→3'exonuclease activity of thermus aquaticus DNA polymerase,Proceedings of the National A-cademy of Sciences,88(16):7276-7280
Horii T.,Ohtsuka H.,Osaki M.,and Ohkuni H.,2009,Use of a dual priming oligonucleotide system to detect multiple sexually transmitted pathogens in clinical specimens,Letters in Applied Microbiology,49(1):46-52
Hu J.Q.,Lei J.T.,Jing J.Z.,Sun X.C.,Gao H.,Geng Y.,Zhang Y.L.,Dong C.W.,and Jiang C.P.,2016,Advance in PCR detection technologies for foodborne pathogenic bacteria,Qinggong Xuebao(Journal of Light Industry),31(3):49-56(胡金强,雷俊婷,景建洲,孙新城,高辉,耿尧,章银良,董彩文,姜春鹏,2016,食源性致病菌PCR检测技术研究进展,轻工学报,31(3):49-56)
Kim S.R.,Ki C.S.,and Lee N.Y.,2009,Rapid detection and identification of 12 respiratory viruses using a dual priming oligonucleotide system-based multiplex PCR assay,Journal of Virological Methods,156(1-2):111-116
Kommedal O.,Simmon K.,Karaca D.,Langeland N.,and Wiker H.G.,2012,Dual priming oligonucleotides for broad-range amplification of the bacterial 16S r RNA gene directly from human clinical specimens,Journal of Clinical Microbiology,50(4):1289-1294
Lee C.S.,Kang B.K.,Lee D.H.,Lyou S.H.,Park B.K.,Ann S.K.,Jung K.,and Song D.S.,2008,One-step multiplex RT-PCRfor detection and subtyping of swine influenza H1,H3,N1,N2 viruses in clinical samples using a dual priming oligonucleotide(DPO)system,Journal of Virological Methods,151(1):30-34
Lee H.R.,Kim S.Y.,Chang H.E.,Song S.H.,Lee H.S.,Park K.U.,Song J.H.,and Kim E.C.,2010,Novel multiplex PCR using dual-priming oligonucleotides for detection and discrimination of the mycobacterium tuberculosis complex and m.bovis bcg,Journal of Clinical Microbiology,48(12):4612-4614
Li D.D.,Xu Y.G.,Chen W.H.,Qiu S.P.,Gao S.Y.,and Li Y.J.,2015,Development of a dual-priming oligonucleotide-based PCR assay for the rapid detection of enteropathogenic Escherichia coli,Zhongguo Yufang Shouyi Xuebao(Chinese Journal of Preventive Veterinary Medicine),37(7):541-544(李丹丹,徐义刚,陈文慧,邱索平,高慎阳,李一经,2015,肠致病性大肠杆菌DPO-PCR快速检测方法的建立,中国预防兽医学报,37(7):541-544)
Li D.D.,Xu Y.G.,Qiu S.P.,Wang Y.,Gao H.J.,and Gao S.Y.,2015,Development and application of DPO-PCR method for the detection of enteroinvasive Escherichia coli,Zhongguo Renshou Gonghuanbing Xuebao(Chinese Journal of Zoonoses),31(9):826-830(李丹丹,徐义刚,邱索平,王昱,高会江,高慎阳,2015,肠侵袭性大肠杆菌DPO-PCR检测方法的建立与应用,中国人兽共患病学报,31(9):826-830)
Li D.D.,Xu Y.G.,Qiu S.P.,Wang Y.,Gao H.J.,and Gao S.Y.,2016,A DPO-PCR method for the rapid detection of Staphylococcus aureus,Shiping Yu Shengwu Jishu Xuebao(Journal of Food Science and Biotechnology),35(4):419-423(李丹丹,徐义刚,邱索平,王昱,高会江,高慎阳,2016,金黄色葡萄球菌DPO-PCR快速检测方法的建立与应用,食品与生物技术学报,35(4):419-423)
Li D.D.,Xu Y.G.,Wang Y.,Gao H.J.,and Gao S.Y.,2015,Development of DPO-PCR method for the detection of Vibrio vulnificus,Shiping Keji(Food Science and Technology),40(11):287-291(李丹丹,徐义刚,王昱,高会江,高慎阳,2015,创伤弧菌DPO-PCR检测方法的建立,食品科技,40(11):287-291)
Li D.D.,Xu Y.G.,Wang Y.,Qiu S.P.,Gao H.J.,and Gao S.Y.,2015,Development of DPO-PCR method for the detection of Vibrio parahaemolyticus,Shiping Yu Fajiao Gongye(Food and Fermentation Industries),41(11):133-136(李丹丹,徐义刚,王昱,邱索平,高会江,高慎阳,2015,副溶血弧菌DPO-PCR检测方法的建立,食品与发酵工业,41(11):133-136)
Liu C.Z.,Wang Y.L.,and Jing J.Z.,2011,Multiplex PCR for detection of Listeria monocytogenes with DPO primers,Hubei Nongye Xuebao(Hubei Agricultural Sciences),50(15):3197-3200(刘纯真,王云龙,景建洲,2011,基于DPO引物的多重PCR在检测单增李斯特菌中的应用,湖北农业科学,50(15):3197-3200)
Liu M.,Huang X.,Ma Z.H.,Chen H.J.,and Li M.F.,2009,Multiplex RT-PCR for detection of potato viruses with a dual priming oligonucleotide(DPO)system,Zhiwu Bingli Xuebao(Acta Phytopathologica Sinica),39(4):431-434(刘梅,黄新,马占鸿,陈洪俊,李明福,2009,应用DPO引物检测马铃薯病毒的多重RT-PCR技术研究,植物病理学报,39(4):431-434)
Lu F.S.,Wang Y.L.,and Jing J.Z.,2012,Multiplex-PCR for detection of foodborne pathogens with a dual priming oligonucleotide(DPO)system,Shiping Gongye Keji(Science and Technology of Food Industry),33(2):199-202(卢富山,王云龙,景建洲,2012,应用DPO技术检测食源性致病菌的多重PCR的研究,食品工业科技,33(2):199-202)
Moon H.J.,Park S.J.,Kim H.K.,Ann S.K.,Rho S.,Keum H.O.,and Park B.K.,2010,Simultaneous detection and subtyping of porcine endogenous re troviruses proviral dna using the dual priming oligonucleotide system,Journal of Veterinary Science,11(3):269-271
Mullis K.B.,1990,The unusual origin of the polymerase chain reaction,Scientific American,262(4):64-65
Saiki R.K.,Gelfand D.H.,Stoffel S.,Scharf S.J.,Higuchi R.,Horn G.T.,Mullis K.B.,and Erlich H.A.,1988,Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase,Science,239(4839):487-491
Shen B.,Wang Z.F.,Hu X.J.,and Yang S.J.,2015,Study on multiplex-PCR for detecting 6 kinds of common pathogenic bacteria with DPO system,Zhongguo Weisheng Jianyan Zazhi(Chinese Journal of Health Laboratory Technology),25(22):3855-3857(沈飚,王忠发,胡兴娟,杨赛军,2015,基于DPO引物构建的六重PCR检测食品中常见致病菌的方法研究,中国卫生检验杂志,25(22):3855-3857)
Su Y.,Li R.,and Chen J.M.,2015,Expression of gfp Gene in Transgenic gfp Barley(Hordeum vulgare L.)by Semi-quantitative RT-PCR Analysis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(9):804-810(苏琰,李融,陈建民,2015,转gfp基因大麦中gfp基因表达的半定量RT-PCR方法分析,基因组学与应用生物学,34(9):1934-1938)
Wang Y.,Ning H.H.,Liang Y.,Wang B.B.,Li B.,and Shen L.X.,2015,The Origin of resistance genes and evolution of antibiotic resistance,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(4):855-861(王严,宁唤唤,梁鹰,王波波,李博,沈立新,2015,细菌耐药性的起源与演变,基因组学与应用生物学,34(4):855-861)
Woo H.Y.,Park D.L.,Park H.,Kim M.K.,Kim D.H.,Kim I.S.,and Kim Y.J.,2009,Dual-priming oligonucleotide-based multiplex PCR for the detection of helicobacter pylori and determination of clarithromycin resistance with gastric biopsy specimens,Helicobacter,14(1):22-28
Woo H.Y.,Park H.,Kim B.I.,Jeon W.K.,and Kim Y.J.,2008,E-valuation of dual priming oligonucleotide(DPO)-based multiplex PCR for detection of HBV YMDD mutants,Archives of Virology,153(11):2019-2025
Xu Y.G.,Li D.D.,Cui L.C.,Liu Z.M.,and Li S.L.,2014,Detection of enterohemorrhagic Escherichia coli O157:H7 by DPO-PCR method,Shiping Kexue(Food Science),35(8):160-164(徐义刚,李丹丹,崔丽春,刘忠梅,李苏龙,2014,应用DPO-PCR技术检测肠出血性大肠杆菌O157:H7,食品科学,35(8):160-164)
Xu Y.G.,Li D.D.,Liu Z.M.,Wu Y.,and Li S.L.,2014,DPO-based PCR method for specific detection of Yersinia enterocolitica,Zhongguo Renshou Gonghuanbing Xuebao(Chinese Journal of Zoonoses),30(5):507-510(徐义刚,李丹丹,刘忠梅,吴岩,李苏龙,2014,基于DPO引物特异性检测小肠结肠炎耶尔森氏菌的PCR方法,中国人兽共患病学报,30(5):507-510)
Xu Y.G.,Li D.D.,Liu Z.M.,Wu Y.,Li S.L.,and Wei D.X.,2014,Specific detection of Shigella using DPO-PCR method,Zhongguo Xumu Shouyi(China Animal Husbandry and Veterinary Medicine),41(3):28-32(徐义刚,李丹丹,刘忠梅,吴岩,李苏龙,魏冬旭,2014,应用DPO-PCR方法特异性检测志贺氏菌,中国畜牧兽医,41(3):28-32)
Xu Y.G.,Li D.D.,Liu Z.M.,Wu Y.,Wei D.X.,and Li S.L.,2014,Development and preliminary application of DPO-based PCR method for detection of Campylobacter jejuni,Zhongguo Yufang Shouyi Xuebao(Chinese Journal of Preventive Veterinary Medicine),36(4):293-296(徐义刚,李丹丹,刘忠梅,吴岩,魏冬旭,李苏龙,2014,基于DPO引物的空肠弯曲菌PCR检测方法的建立与初步应用,中国预防兽医学报,36(4):293-296)
Xu Y.G.,Li D.D.,Wu Y.,Liu Z.M.,Wei D.X.,and Li S.L.,2014,Develoment and application of DPO-PCR method for specific detection of Vibrio cholerae,Zhongguo Shouyi Xuebao(Chinese Journal of Veterinary Science),34(8):1284-1288(徐义刚,李丹丹,吴岩,刘忠梅,魏冬旭,李苏龙,2014,霍乱弧菌DPO-PCR特异性检测方法的建立与应用,中国兽医学报,34(8):1284-1288)
Yoo S.J.,Kuak E.Y.,and Shin B.M.,2007,Detection of 12 respiratory viruses with two-set multiplex reverse transcriptase-pcr assay using a dual priming oligonucleotide system,Korean Journal Laboratory Medicine,27(6):420-427
Zhang N.,Qian Y.K.,Wei S.,Liang Q.L.,Yin J.P.,Liu Z.Y.,and Chen W.M.,2015,Multiplex DPO-PCR detection of two quarantine fungal diseases of sunflower,Zhiwu Jianyi(Plant Quarantine),29(6):35-38(张娜,乾义柯,魏霜,梁巧玲,鄞杰平,刘中勇,陈卫民,2015,两种向日葵检疫性真菌病害的多重DPO-PCR检测方法,植物检疫,29(6):35-38)
Carrara L.,Navarro F.,Turbau M.,Seres M.,Morán I.,Quintana I.,Martino R.,González Y.,Brell A.,Cordon O.,Diestra K.,Mata C.,Mirelis B.,and Coll P.,2013,Molecular diagnosis of bloodstream infections with a new dual-priming oligonucleotide-based multiplex PCR assay,Journal of Medical Microbiology,62:1673-1679
Chamberlain J.S.,Gibbs R.A.,Ranier J.E.,Nguyen P.N.,and Caskey C.T.,1988,Deletion screening of the duchenne muscular dystrophy locus via multiplex DNA amplification,Nucleic Acids Research,16(23):11141-11156
Chun J.Y.,Kim K.J.,Hwang I.T.,Kim Y.J.,Lee D.H.,Lee I.K.,an d Kim J.K.,2007,Dual priming oligonucleotide system for the multiplex detection of respiratory viruses and snp genotyping of CYP2C19 gene,Nucleic Acids Research,35(6):e40
Chung W.C.,Jung S.H.,Oh J.H.,Kim T.H.,Cheung D.Y.,Kim B.W.,Kim S.S.,Kim J.I.,and Sin E.Y.,2014,Dual-priming oligonucleotide-based multiplex PCR using tissue samples in rapid urease test in the detection of helicobacter pylori infection,World Journal of Gastroenterology,20(21):6547-6553
Chung W.C,Jeon E.J.,Oh J.H.,Park J.M.,Kim T.H.,Cheung D.Y.,Kim B.W.,Kim S.S.,and Kim J.I.,2016,Dual-priming oligonucleotide-based multiplex PCR using tissue samples from the rapid urease test kit for the detection of Helicobacter pylori in bleedingpeptic ulcers,Digestive and Liver Disease,48(8):899-903
Heid C.A.,Stevens J.,Livak K.J.,and Williams P.M.,1996,Real time quantitative PCR,Genome Research,6(10):986-994
Higuchi R.,Fockler C.,Dollinger G.,and Watson R.,1993,Kinetic pcr analysis:Real-time monitoring of DNA amplification reactions,Nature Biotechnolgy,11(9):1026-1030
Higuchi R.,Dollinger G.,Walsh P.S.,a nd Griffi th R.,1992,Simultaneous amplification and detection of specific DNA sequences,Bio/technolgy,10(4):413-417
Holland P.M.,Abramson R.D.,Watson R.,and Gelfand D.H.,1991,Detection ofspecific polymerase chain reaction product by utilizing the 5'→3'exonuclease activity of thermus aquaticus DNA polymerase,Proceedings of the National A-cademy of Sciences,88(16):7276-7280
Horii T.,Ohtsuka H.,Osaki M.,and Ohkuni H.,2009,Use of a dual priming oligonucleotide system to detect multiple sexually transmitted pathogens in clinical specimens,Letters in Applied Microbiology,49(1):46-52
Hu J.Q.,Lei J.T.,Jing J.Z.,Sun X.C.,Gao H.,Geng Y.,Zhang Y.L.,Dong C.W.,and Jiang C.P.,2016,Advance in PCR detection technologies for foodborne pathogenic bacteria,Qinggong Xuebao(Journal of Light Industry),31(3):49-56(胡金强,雷俊婷,景建洲,孙新城,高辉,耿尧,章银良,董彩文,姜春鹏,2016,食源性致病菌PCR检测技术研究进展,轻工学报,31(3):49-56)
Kim S.R.,Ki C.S.,and Lee N.Y.,2009,Rapid detection and identification of 12 respiratory viruses using a dual priming oligonucleotide system-based multiplex PCR assay,Journal of Virological Methods,156(1-2):111-116
Kommedal O.,Simmon K.,Karaca D.,Langeland N.,and Wiker H.G.,2012,Dual priming oligonucleotides for broad-range amplification of the bacterial 16S r RNA gene directly from human clinical specimens,Journal of Clinical Microbiology,50(4):1289-1294
Lee C.S.,Kang B.K.,Lee D.H.,Lyou S.H.,Park B.K.,Ann S.K.,Jung K.,and Song D.S.,2008,One-step multiplex RT-PCRfor detection and subtyping of swine influenza H1,H3,N1,N2 viruses in clinical samples using a dual priming oligonucleotide(DPO)system,Journal of Virological Methods,151(1):30-34
Lee H.R.,Kim S.Y.,Chang H.E.,Song S.H.,Lee H.S.,Park K.U.,Song J.H.,and Kim E.C.,2010,Novel multiplex PCR using dual-priming oligonucleotides for detection and discrimination of the mycobacterium tuberculosis complex and m.bovis bcg,Journal of Clinical Microbiology,48(12):4612-4614
Li D.D.,Xu Y.G.,Chen W.H.,Qiu S.P.,Gao S.Y.,and Li Y.J.,2015,Development of a dual-priming oligonucleotide-based PCR assay for the rapid detection of enteropathogenic Escherichia coli,Zhongguo Yufang Shouyi Xuebao(Chinese Journal of Preventive Veterinary Medicine),37(7):541-544(李丹丹,徐义刚,陈文慧,邱索平,高慎阳,李一经,2015,肠致病性大肠杆菌DPO-PCR快速检测方法的建立,中国预防兽医学报,37(7):541-544)
Li D.D.,Xu Y.G.,Qiu S.P.,Wang Y.,Gao H.J.,and Gao S.Y.,2015,Development and application of DPO-PCR method for the detection of enteroinvasive Escherichia coli,Zhongguo Renshou Gonghuanbing Xuebao(Chinese Journal of Zoonoses),31(9):826-830(李丹丹,徐义刚,邱索平,王昱,高会江,高慎阳,2015,肠侵袭性大肠杆菌DPO-PCR检测方法的建立与应用,中国人兽共患病学报,31(9):826-830)
Li D.D.,Xu Y.G.,Qiu S.P.,Wang Y.,Gao H.J.,and Gao S.Y.,2016,A DPO-PCR method for the rapid detection of Staphylococcus aureus,Shiping Yu Shengwu Jishu Xuebao(Journal of Food Science and Biotechnology),35(4):419-423(李丹丹,徐义刚,邱索平,王昱,高会江,高慎阳,2016,金黄色葡萄球菌DPO-PCR快速检测方法的建立与应用,食品与生物技术学报,35(4):419-423)
Li D.D.,Xu Y.G.,Wang Y.,Gao H.J.,and Gao S.Y.,2015,Development of DPO-PCR method for the detection of Vibrio vulnificus,Shiping Keji(Food Science and Technology),40(11):287-291(李丹丹,徐义刚,王昱,高会江,高慎阳,2015,创伤弧菌DPO-PCR检测方法的建立,食品科技,40(11):287-291)
Li D.D.,Xu Y.G.,Wang Y.,Qiu S.P.,Gao H.J.,and Gao S.Y.,2015,Development of DPO-PCR method for the detection of Vibrio parahaemolyticus,Shiping Yu Fajiao Gongye(Food and Fermentation Industries),41(11):133-136(李丹丹,徐义刚,王昱,邱索平,高会江,高慎阳,2015,副溶血弧菌DPO-PCR检测方法的建立,食品与发酵工业,41(11):133-136)
Liu C.Z.,Wang Y.L.,and Jing J.Z.,2011,Multiplex PCR for detection of Listeria monocytogenes with DPO primers,Hubei Nongye Xuebao(Hubei Agricultural Sciences),50(15):3197-3200(刘纯真,王云龙,景建洲,2011,基于DPO引物的多重PCR在检测单增李斯特菌中的应用,湖北农业科学,50(15):3197-3200)
Liu M.,Huang X.,Ma Z.H.,Chen H.J.,and Li M.F.,2009,Multiplex RT-PCR for detection of potato viruses with a dual priming oligonucleotide(DPO)system,Zhiwu Bingli Xuebao(Acta Phytopathologica Sinica),39(4):431-434(刘梅,黄新,马占鸿,陈洪俊,李明福,2009,应用DPO引物检测马铃薯病毒的多重RT-PCR技术研究,植物病理学报,39(4):431-434)
Lu F.S.,Wang Y.L.,and Jing J.Z.,2012,Multiplex-PCR for detection of foodborne pathogens with a dual priming oligonucleotide(DPO)system,Shiping Gongye Keji(Science and Technology of Food Industry),33(2):199-202(卢富山,王云龙,景建洲,2012,应用DPO技术检测食源性致病菌的多重PCR的研究,食品工业科技,33(2):199-202)
Moon H.J.,Park S.J.,Kim H.K.,Ann S.K.,Rho S.,Keum H.O.,and Park B.K.,2010,Simultaneous detection and subtyping of porcine endogenous re troviruses proviral dna using the dual priming oligonucleotide system,Journal of Veterinary Science,11(3):269-271
Mullis K.B.,1990,The unusual origin of the polymerase chain reaction,Scientific American,262(4):64-65
Saiki R.K.,Gelfand D.H.,Stoffel S.,Scharf S.J.,Higuchi R.,Horn G.T.,Mullis K.B.,and Erlich H.A.,1988,Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase,Science,239(4839):487-491
Shen B.,Wang Z.F.,Hu X.J.,and Yang S.J.,2015,Study on multiplex-PCR for detecting 6 kinds of common pathogenic bacteria with DPO system,Zhongguo Weisheng Jianyan Zazhi(Chinese Journal of Health Laboratory Technology),25(22):3855-3857(沈飚,王忠发,胡兴娟,杨赛军,2015,基于DPO引物构建的六重PCR检测食品中常见致病菌的方法研究,中国卫生检验杂志,25(22):3855-3857)
Su Y.,Li R.,and Chen J.M.,2015,Expression of gfp Gene in Transgenic gfp Barley(Hordeum vulgare L.)by Semi-quantitative RT-PCR Analysis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(9):804-810(苏琰,李融,陈建民,2015,转gfp基因大麦中gfp基因表达的半定量RT-PCR方法分析,基因组学与应用生物学,34(9):1934-1938)
Wang Y.,Ning H.H.,Liang Y.,Wang B.B.,Li B.,and Shen L.X.,2015,The Origin of resistance genes and evolution of antibiotic resistance,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(4):855-861(王严,宁唤唤,梁鹰,王波波,李博,沈立新,2015,细菌耐药性的起源与演变,基因组学与应用生物学,34(4):855-861)
Woo H.Y.,Park D.L.,Park H.,Kim M.K.,Kim D.H.,Kim I.S.,and Kim Y.J.,2009,Dual-priming oligonucleotide-based multiplex PCR for the detection of helicobacter pylori and determination of clarithromycin resistance with gastric biopsy specimens,Helicobacter,14(1):22-28
Woo H.Y.,Park H.,Kim B.I.,Jeon W.K.,and Kim Y.J.,2008,E-valuation of dual priming oligonucleotide(DPO)-based multiplex PCR for detection of HBV YMDD mutants,Archives of Virology,153(11):2019-2025
Xu Y.G.,Li D.D.,Cui L.C.,Liu Z.M.,and Li S.L.,2014,Detection of enterohemorrhagic Escherichia coli O157:H7 by DPO-PCR method,Shiping Kexue(Food Science),35(8):160-164(徐义刚,李丹丹,崔丽春,刘忠梅,李苏龙,2014,应用DPO-PCR技术检测肠出血性大肠杆菌O157:H7,食品科学,35(8):160-164)
Xu Y.G.,Li D.D.,Liu Z.M.,Wu Y.,and Li S.L.,2014,DPO-based PCR method for specific detection of Yersinia enterocolitica,Zhongguo Renshou Gonghuanbing Xuebao(Chinese Journal of Zoonoses),30(5):507-510(徐义刚,李丹丹,刘忠梅,吴岩,李苏龙,2014,基于DPO引物特异性检测小肠结肠炎耶尔森氏菌的PCR方法,中国人兽共患病学报,30(5):507-510)
Xu Y.G.,Li D.D.,Liu Z.M.,Wu Y.,Li S.L.,and Wei D.X.,2014,Specific detection of Shigella using DPO-PCR method,Zhongguo Xumu Shouyi(China Animal Husbandry and Veterinary Medicine),41(3):28-32(徐义刚,李丹丹,刘忠梅,吴岩,李苏龙,魏冬旭,2014,应用DPO-PCR方法特异性检测志贺氏菌,中国畜牧兽医,41(3):28-32)
Xu Y.G.,Li D.D.,Liu Z.M.,Wu Y.,Wei D.X.,and Li S.L.,2014,Development and preliminary application of DPO-based PCR method for detection of Campylobacter jejuni,Zhongguo Yufang Shouyi Xuebao(Chinese Journal of Preventive Veterinary Medicine),36(4):293-296(徐义刚,李丹丹,刘忠梅,吴岩,魏冬旭,李苏龙,2014,基于DPO引物的空肠弯曲菌PCR检测方法的建立与初步应用,中国预防兽医学报,36(4):293-296)
Xu Y.G.,Li D.D.,Wu Y.,Liu Z.M.,Wei D.X.,and Li S.L.,2014,Develoment and application of DPO-PCR method for specific detection of Vibrio cholerae,Zhongguo Shouyi Xuebao(Chinese Journal of Veterinary Science),34(8):1284-1288(徐义刚,李丹丹,吴岩,刘忠梅,魏冬旭,李苏龙,2014,霍乱弧菌DPO-PCR特异性检测方法的建立与应用,中国兽医学报,34(8):1284-1288)
Yoo S.J.,Kuak E.Y.,and Shin B.M.,2007,Detection of 12 respiratory viruses with two-set multiplex reverse transcriptase-pcr assay using a dual priming oligonucleotide system,Korean Journal Laboratory Medicine,27(6):420-427
Zhang N.,Qian Y.K.,Wei S.,Liang Q.L.,Yin J.P.,Liu Z.Y.,and Chen W.M.,2015,Multiplex DPO-PCR detection of two quarantine fungal diseases of sunflower,Zhiwu Jianyi(Plant Quarantine),29(6):35-38(张娜,乾义柯,魏霜,梁巧玲,鄞杰平,刘中勇,陈卫民,2015,两种向日葵检疫性真菌病害的多重DPO-PCR检测方法,植物检疫,29(6):35-38)