生物功能化AgInS_2量子点的制备及其生物应用
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摘要
为制备水相、低毒、生物兼容性较好的三元量子点,通过水相合成法制备三元银铟硫(AgInS_2)量子点,并用叶酸(FA)对其进行修饰,利用透射电子显微镜、紫外-可见-近红外分光光度计、荧光分光光度计、傅里叶红外光谱仪等表征手段对纳米复合材料的形貌、光学性质、化学组成、细胞毒性及荧光成像进行了研究。结果表明,水相AgInS_2量子点的尺寸约为5nm~6nm,经叶酸(FA)修饰后,细胞毒性较低,在乳腺癌细胞中成像时红色荧光更明显,说明通过叶酸的修饰可提高量子点的生物兼容性。因此这类具有较好水溶性和生物兼容性的FA-AgInS_2量子点可直接应用于生物成像研究,为乳腺癌的快速体外检测打下了良好的实验基础。
In order to synthesized ternary quantum dots with good water solubility,low toxicity and strong biocompatibility,AgInS_2 quantum dots were synthesized by aqueous phase synthesis and modified with folic acid(FA). Transmission Electron Microscope, UV-Vis-NIR Spectrophotometer, Fluorescence Spectrophotometer, Infrared Spectrometer,Fourier transform infrared spectroscopy and other characterization methods were used to study the morphology,optical properties,chemical composition,cytotoxicity and fluorescence imaging of nanocomposites. The results showed that the size of the AgInS_2 quantum dots in water was about 5~6 nm,and the cytotoxicity was lower after folic acid(FA)modification. The red fluorescence in the breast cancer cells was more obvious,indicating that the biocompatibility of quantum dots can be improved by folic acid modification. Therefore,these FA-AgInS_2 quantum dots with good water solubility and biocompatibility can be directly applied to the research of biological imaging,which lays a good foundation for rapid and in vitro detection of breast cancer.
In order to synthesized ternary quantum dots with good water solubility,low toxicity and strong biocompatibility,AgInS_2 quantum dots were synthesized by aqueous phase synthesis and modified with folic acid(FA). Transmission Electron Microscope, UV-Vis-NIR Spectrophotometer, Fluorescence Spectrophotometer, Infrared Spectrometer,Fourier transform infrared spectroscopy and other characterization methods were used to study the morphology,optical properties,chemical composition,cytotoxicity and fluorescence imaging of nanocomposites. The results showed that the size of the AgInS_2 quantum dots in water was about 5~6 nm,and the cytotoxicity was lower after folic acid(FA)modification. The red fluorescence in the breast cancer cells was more obvious,indicating that the biocompatibility of quantum dots can be improved by folic acid modification. Therefore,these FA-AgInS_2 quantum dots with good water solubility and biocompatibility can be directly applied to the research of biological imaging,which lays a good foundation for rapid and in vitro detection of breast cancer.
引文
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[3]毕克,张铁强,张宇.Zn Cu In S/Zn S量子点荧光粉LED发光特性研究[J].长春理工大学学报:自然科学版,2014,37(4):44-47.
[4]Hamanaka Y,Ogawa T,Tsuzuki M,et al.Photoluminescence properties and its origin of Ag In S2quantum dots with chalcopyrite structure[J].Journal of Physical Chemistry C,2011,115(5):1786-1792.
[5]杨帆.Ag In S2量子点的光物理特性研究[D].长春:吉林大学,2016.
[6]Zhong H,Bai Z,Zou B.Tuning the luminescence properties of colloidal I-III-VI semiconductor nanocrystals for optoelectronics and biotechnology applications[J].Journal of Physical Chemistry Letters,2012,3(21):3167-3175.
[7]Wang Y,Zhang Q,Li Y,et al.Preparation of Ag In S2quantum dot/In2S3co-sensitized photoelectrodes by a facile aqueous-phase synthesis route and their photovoltaic performance[J].Nanoscale,2015,7(14):6185-6192.
[8]谢翠萍,向卫东,骆乐,等.Ag In S_2量子点研究进展[J].功能材料,2014,45(04):4009-4016.
[9]Chang J Y,Wang G Q,Cheng C Y,et al.Strategies for photoluminescence enhancement of Ag In S2quantum dots and their application as bioimaging probes[J].Journal of Materials Chemistry,2012,22(21):10609-10618.
[10]Hong S P,Park H K,Ji H O,et al.Comparisons of the structural and optical properties of o-Ag In S2,t-Ag In S2,and c-Ag In5S8 nanocrystals and their solid-solution nanocrystals with Zn S[J].Journal of Materials Chemistry,2012,22(36):18939-18949.
[11]Liu L,Hu R,Roy I,et al.Synthesis of luminescent near-infrared Ag In S2nanocrystals as optical probes for in vivo applications[J].Theranostics,2013,3(2):109.
[12]Chaudhuri R G,Paria S.Core/Shell nanoparticles:classes,properties,synthesis mechanisms,characterization,and applications[J].Chemical Reviews,2011,112(4):2373-433.
[13]Kang X,Huang L,Yang Y,et al.Scaling up the aqueous synthesis of visible light emitting multinary Ag In S2/Zn S core/shell quantum dots[J].Journal of Physical Chemistry C,2015,119(14):7933-7940.
[14]Wu P J,Ou K L,Chen J K,et al.Methotrexate-conjugated Ag In S2/Zn S quantum dots for optical imaging and drug delivery[J].Materials Letters,2014,128(8):412-416.
[15]Song J,Jiang T,Guo T,et al.Facile synthesis of water-soluble Zn-doped Ag In5S8/Zn S core/shell fluorescent nanocrystals and their biological application[J].Inorganic Chemistry,2015,54(4):1627-1633.
[16]Dai M,Ogawa S,Kameyama T,et al.Tunable photoluminescence from the visible to near-infrared wavelength region of non-stoichiometric Ag In S2nanoparticles[J].Journal of Materials Chemistry,2012,22(25):12851-12858.
[17]吕鉴泉,胡芹芹,丁然,等.核壳结构Ag In S2@Zn S量子点的合成及荧光性能[J].高等学校化学学报,2013,34(11):2478-2482.
[18]卜承飞,刘丽炜,王倩,等.叶酸修饰的Ag In S_2量子点在体外癌细胞成像上的应用[J].发光学报,2015,36(09):989-995.
[19]胡思怡.基于微流控技术的功能型量子点的合成及应用[D].长春:长春理工大学,2015.